##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AccII AccIII AclI AgeI Alw44I
Aor51HI ApaLI AscI AspI BamHI
BbeI BbrPI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BsrGI BssHII BssSI BstBI
BstUI CelII ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoRI
FseI HaeII HpaI KasI KspI
MamI MfeI MluI MroI MunI
MvnI NarI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 4453 GACGTC GACGT^C
Acc65I 1784 GGTACC G^GTACC
AflIII 4882 ACATGT A^C[GA][CT]GT
AhdI 2425 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1579 CCTGAGG CC^T[ACGT]AGG
ApaI 432 GGGCCC GGGCC^C
Asp700I 1666 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1784 GGTACC G^GTACC
AspEI 2425 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4354 TGCGCA TGC^GCA
AvrII 3918 CCTAGG C^CTAGG
BalI 1653 TGGCCA TGG^CCA
BbvCI 491 CCTCAGC CCTCAGC(-5/-2)
BcgI 392 CGACCAAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4633 GGATAC GTATCC(6/5)
BclI 144 TGATCA T^GATCA
BcnI 2079 CCGGG CC^[GC]GG
BlnI 3918 CCTAGG C^CTAGG
BsaAI 317 TACGTG [CT]AC^GT[GA]
BspLU11I 4882 ACATGT A^CATGT
BsrBI 480 CCGCTC CCGCTC(-3/-3)
BsrFI 4434 GCCGGC [GA]^CCGG[CT]
Bst1107I 5294 GTATAC GTA^TAC
BstZ17I 5294 GTATAC GTA^TAC
Bsu36I 1579 CCTGAGG CC^T[ACGT]AGG
Cfr10I 4434 GCCGGC [GA]^CCGG[CT]
DraIII 1036 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3579 CCATGG C^C[GA][CT]GG
Eam1105I 2425 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1579 CCTGAGG CC^T[ACGT]AGG
EcoRV 2563 GATATC GAT^ATC
FbaI 144 TGATCA T^GATCA
FspI 4354 TGCGCA TGC^GCA
HgaI 2418 GCGTC GACGC(5/10)
HindIII 3826 AAGCTT A^AGCTT
KpnI 1784 GGTACC GGTAC^C
MluNI 1653 TGGCCA TGG^CCA
MscI 1653 TGGCCA TGG^CCA
MslI 3262 CACTGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 4434 GCCGGC GCC^GGC
NciI 2079 CCGGG CC^[GC]GG
NcoI 3579 CCATGG C^CATGG
NdeI 4138 CATATG CA^TATG
NgoMIV 4434 GCCGGC G^CCGGC
PflMI 3151 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI 851 GTCGAC G^TCGAC
SexAI 1569 ACCTGGT A^CC[AT]GGT
SpeI 2747 ACTAGT A^CTAGT
StuI 760 AGGCCT AGG^CCT
Van91I 3151 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1666 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 2417 GGCGTC G[GA]^CG[CT]C
4453 GACGTC
AlwNI 4022 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
4076 CAGATGCTG
AseI 3163 ATTAAT AT^TAAT
4318 ATTAAT
AsnI 3163 ATTAAT AT^TAAT
4318 ATTAAT
BglI 2409 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3992 GCCTCTCTGGC
BglII 3769 AGATCT A^GATCT
4232 AGATCT
BsaHI 2417 GGCGTC G[GA]^CG[CT]C
4453 GACGTC
BsiEI 98 CGGTCG CG[GA][CT]^CG
1135 CGACCG
BstEII 2122 GGTGACC G^GT[ACGT]ACC
3602 GGTGACC
BstPI 2122 GGTGACC G^GT[ACGT]ACC
3602 GGTGACC
EaeI 1653 TGGCCA [CT]^GGCC[GA]
2073 TGGCCG
EcoNI 4249 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5089 CCTCTGCTAGG
EcoO65I 2122 GGTGACC G^GT[ACGT]ACC
3602 GGTGACC
EcoT22I 4666 ATGCAT ATGCA^T
5210 ATGCAT
Hin1I 2417 GGCGTC G[GA]^CG[CT]C
4453 GACGTC
NsiI 4666 ATGCAT ATGCA^T
5210 ATGCAT
PshBI 3163 ATTAAT AT^TAAT
4318 ATTAAT
PstI 400 CTGCAG CTGCA^G
2591 CTGCAG
ScaI 3903 AGTACT AGT^ACT
4997 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BfaI BfrI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NheI NlaIII NlaIV NspI
PleI PpuMI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII