################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AccII AccIII AclI AgeI Alw44I Aor51HI ApaLI AscI AspI BamHI BbeI BbrPI BlpI Bpu1102I BsaBI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BsrGI BssHII BssSI BstBI BstUI CelII ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoRI FseI HaeII HpaI KasI KspI MamI MfeI MluI MroI MunI MvnI NarI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 4453 GACGTC GACGT^C Acc65I 1784 GGTACC G^GTACC AflIII 4882 ACATGT A^C[GA][CT]GT AhdI 2425 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1579 CCTGAGG CC^T[ACGT]AGG ApaI 432 GGGCCC GGGCC^C Asp700I 1666 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1784 GGTACC G^GTACC AspEI 2425 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 4354 TGCGCA TGC^GCA AvrII 3918 CCTAGG C^CTAGG BalI 1653 TGGCCA TGG^CCA BbvCI 491 CCTCAGC CCTCAGC(-5/-2) BcgI 392 CGACCAAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 4633 GGATAC GTATCC(6/5) BclI 144 TGATCA T^GATCA BcnI 2079 CCGGG CC^[GC]GG BlnI 3918 CCTAGG C^CTAGG BsaAI 317 TACGTG [CT]AC^GT[GA] BspLU11I 4882 ACATGT A^CATGT BsrBI 480 CCGCTC CCGCTC(-3/-3) BsrFI 4434 GCCGGC [GA]^CCGG[CT] Bst1107I 5294 GTATAC GTA^TAC BstZ17I 5294 GTATAC GTA^TAC Bsu36I 1579 CCTGAGG CC^T[ACGT]AGG Cfr10I 4434 GCCGGC [GA]^CCGG[CT] DraIII 1036 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 3579 CCATGG C^C[GA][CT]GG Eam1105I 2425 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1579 CCTGAGG CC^T[ACGT]AGG EcoRV 2563 GATATC GAT^ATC FbaI 144 TGATCA T^GATCA FspI 4354 TGCGCA TGC^GCA HgaI 2418 GCGTC GACGC(5/10) HindIII 3826 AAGCTT A^AGCTT KpnI 1784 GGTACC GGTAC^C MluNI 1653 TGGCCA TGG^CCA MscI 1653 TGGCCA TGG^CCA MslI 3262 CACTGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 4434 GCCGGC GCC^GGC NciI 2079 CCGGG CC^[GC]GG NcoI 3579 CCATGG C^CATGG NdeI 4138 CATATG CA^TATG NgoMIV 4434 GCCGGC G^CCGGC PflMI 3151 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SalI 851 GTCGAC G^TCGAC SexAI 1569 ACCTGGT A^CC[AT]GGT SpeI 2747 ACTAGT A^CTAGT StuI 760 AGGCCT AGG^CCT Van91I 3151 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1666 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcyI 2417 GGCGTC G[GA]^CG[CT]C 4453 GACGTC AlwNI 4022 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG 4076 CAGATGCTG AseI 3163 ATTAAT AT^TAAT 4318 ATTAAT AsnI 3163 ATTAAT AT^TAAT 4318 ATTAAT BglI 2409 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3992 GCCTCTCTGGC BglII 3769 AGATCT A^GATCT 4232 AGATCT BsaHI 2417 GGCGTC G[GA]^CG[CT]C 4453 GACGTC BsiEI 98 CGGTCG CG[GA][CT]^CG 1135 CGACCG BstEII 2122 GGTGACC G^GT[ACGT]ACC 3602 GGTGACC BstPI 2122 GGTGACC G^GT[ACGT]ACC 3602 GGTGACC EaeI 1653 TGGCCA [CT]^GGCC[GA] 2073 TGGCCG EcoNI 4249 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5089 CCTCTGCTAGG EcoO65I 2122 GGTGACC G^GT[ACGT]ACC 3602 GGTGACC EcoT22I 4666 ATGCAT ATGCA^T 5210 ATGCAT Hin1I 2417 GGCGTC G[GA]^CG[CT]C 4453 GACGTC NsiI 4666 ATGCAT ATGCA^T 5210 ATGCAT PshBI 3163 ATTAAT AT^TAAT 4318 ATTAAT PstI 400 CTGCAG CTGCA^G 2591 CTGCAG ScaI 3903 AGTACT AGT^ACT 4997 AGTACT ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AluI AlwI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BfaI BfrI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NheI NlaIII NlaIV NspI PleI PpuMI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII