##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AscI AseI AsnI Asp718I
BbeI BclI BfrI BseAI BsiWI
Bsp1407I BspEI BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I CpoI
DrdI EagI EclXI Eco52I EcoRI
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI NarI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1389 GACGTC GACGT^C
AcyI 1389 GACGTC G[GA]^CG[CT]C
AhdI 3986 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 4007 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3986 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2923 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2756 TGCGCA TGC^GCA
AvrII 113 CCTAGG C^CTAGG
BbrPI 4505 CACGTG CAC^GTG
BglII 2885 AGATCT A^GATCT
BlnI 113 CCTAGG C^CTAGG
BsaHI 1389 GACGTC G[GA]^CG[CT]C
BsaWI 870 ACCGGA [AT]^CCGG[AT]
BsiEI 402 CGACCG CG[GA][CT]^CG
BsmBI 1126 CGTCTC CGTCTC(1/5)
BspDI 2560 ATCGAT AT^CGAT
BspLU11I 3069 ACATGT A^CATGT
BsrDI 590 CATTGC GCAATG(2/0)
BstAPI 1509 GCACAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 2560 ATCGAT AT^CGAT
DraI 3651 TTTAAA TTT^AAA
Eam1105I 3986 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 515 CCTCGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3480 GATATC GAT^ATC
FspI 2756 TGCGCA TGC^GCA
Hin1I 1389 GACGTC G[GA]^CG[CT]C
NdeI 3403 CATATG CA^TATG
PaeR7I 2745 CTCGAG C^TCGAG
PflFI 2923 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4505 CACGTG CAC^GTG
PmlI 4505 CACGTG CAC^GTG
PstI 416 CTGCAG CTGCA^G
SphI 2645 GCATGC GCATG^C
StuI 298 AGGCCT AGG^CCT
Tth111I 2923 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 40 TCTAGA T^CTAGA
XhoI 2745 CTCGAG C^TCGAG
XmnI 4007 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 579 AAATTT [GA]^AATT[CT]
3823 AAATTC
AflIII 3069 ACATGT A^C[GA][CT]GT
3085 ACGTGT
Aor51HI 965 AGCGCT AGC^GCT
3880 AGCGCT
ApaI 1330 GGGCCC GGGCC^C
1529 GGGCCC
ApoI 579 AAATTT [GA]^AATT[CT]
3823 AAATTC
BbvCI 2426 CCTCAGC CCTCAGC(-5/-2)
3860 CCTCAGC
BcgI 2747 CGAGAGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2552 GCAGCAGCATCG
BglI 1987 GCCTAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3668 GCCAATAGGGC
BsaAI 3018 CACGTA [CT]AC^GT[GA]
4505 CACGTG
BsaBI 546 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
715 GATAACCATC
BsmI 373 GAATGC GAATGC(1/-1)
328 GCATTC
BspHI 2220 TCATGA T^CATGA
2794 TCATGA
BstEII 465 GGTGACC G^GT[ACGT]ACC
2991 GGTGACC
BstPI 465 GGTGACC G^GT[ACGT]ACC
2991 GGTGACC
DraIII 1510 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
4505 CACGTGGTG
Eco47III 965 AGCGCT AGC^GCT
3880 AGCGCT
EcoO65I 465 GGTGACC G^GT[ACGT]ACC
2991 GGTGACC
MamI 546 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
715 GATAACCATC
NaeI 2630 GCCGGC GCC^GGC
2668 GCCGGC
NcoI 1316 CCATGG C^CATGG
1903 CCATGG
NgoMIV 2630 GCCGGC G^CCGGC
2668 GCCGGC
NspI 2645 GCATGC [GA]CATG^[CT]
3069 ACATGT
RcaI 2220 TCATGA T^CATGA
2794 TCATGA
SapI 348 GCTCTTC GCTCTTC(1/4)
1545 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwI AlwNI AocI ApaLI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvI BciVI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI