##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AgeI AscI AseI AsnI
Asp700I Asp718I AspI BbrPI BclI
BsaAI BsaBI BsiWI BspDI BspHI
BssHII BstBI BstEII BstPI ClaI
CpoI DraI DraIII EcoO65I EcoRI
EcoRV FbaI HpaI KpnI MamI
MfeI MluI MunI NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RcaI RsrII SacI SalI
SfiI SfuI SgrAI SnaBI SspI
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 2900 GACGTC GACGT^C
AccIII 2971 TCCGGA T^CCGGA
AclI 5427 AACGTT AA^CGTT
AflII 5630 CTTAAG C^TTAAG
AhdI 1501 GACCCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1530 GTGCAC G^TGCAC
ApaLI 1530 GTGCAC G^TGCAC
AspEI 1501 GACCCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4139 TGCGCA TGC^GCA
AvrII 4661 CCTAGG C^CTAGG
BciVI 4454 GTATCC GTATCC(6/5)
BfrI 5630 CTTAAG C^TTAAG
BglII 651 AGATCT A^GATCT
BlnI 4661 CCTAGG C^CTAGG
BseAI 2971 TCCGGA T^CCGGA
BsiEI 3190 CGGCCG CG[GA][CT]^CG
BspEI 2971 TCCGGA T^CCGGA
BspLU11I 524 ACATGT A^CATGT
Bst1107I 5502 GTATAC GTA^TAC
BstZ17I 5502 GTATAC GTA^TAC
DrdI 801 GACTTCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3190 CGGCCG C^GGCCG
Eam1105I 1501 GACCCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3190 CGGCCG C^GGCCG
Eco52I 3190 CGGCCG C^GGCCG
FseI 3331 GGCCGGCC GGCCGG^CC
FspI 4139 TGCGCA TGC^GCA
HgaI 5246 GCGTC GACGC(5/10)
HindIII 2473 AAGCTT A^AGCTT
MroI 2971 TCCGGA T^CCGGA
PaeR7I 3997 CTCGAG C^TCGAG
Psp1406I 5427 AACGTT AA^CGTT
SapI 4585 GAAGAGC GCTCTTC(1/4)
SbfI 1494 CCTGCAGG CCTGCA^GG
SpeI 5553 ACTAGT A^CTAGT
Sse8387I 1494 CCTGCAGG CCTGCA^GG
XhoI 3997 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 2721 GTCTAC GT^[AC][GT]AC
5502 GTATAC
AcsI 592 AAATTT [GA]^AATT[CT]
4512 GAATTT
AflIII 524 ACATGT A^C[GA][CT]GT
5459 ACGTGT
Aor51HI 144 AGCGCT AGC^GCT
2258 AGCGCT
ApoI 592 AAATTT [GA]^AATT[CT]
4512 GAATTT
BamHI 2336 GGATCC G^GATCC
3498 GGATCC
BbeI 775 GGCGCC GGCGC^C
4965 GGCGCC
BsaWI 2273 TCCGGT [AT]^CCGG[AT]
2971 TCCGGA
BsmBI 2582 GAGACG CGTCTC(1/5)
2898 GAGACG
BsmI 4249 GAATGC GAATGC(1/-1)
5661 GCATTC
Bsp1407I 5159 TGTACA T^GTACA
5434 TGTACA
BsrBI 2007 CCGCTC CCGCTC(-3/-3)
607 GAGCGG
BsrGI 5159 TGTACA T^GTACA
5434 TGTACA
Eco47III 144 AGCGCT AGC^GCT
2258 AGCGCT
EcoT22I 2709 ATGCAT ATGCA^T
3799 ATGCAT
HincII 460 GTCAAC GT[CT]^[GA]AC
3634 GTCAAC
HindII 460 GTCAAC GT[CT]^[GA]AC
3634 GTCAAC
KasI 775 GGCGCC G^GCGCC
4965 GGCGCC
KspI 619 CCGCGG CCGC^GG
2781 CCGCGG
MslI 1117 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5090 CACCAACATG
NarI 775 GGCGCC GG^CGCC
4965 GGCGCC
NdeI 3621 CATATG CA^TATG
5438 CATATG
NsiI 2709 ATGCAT ATGCA^T
3799 ATGCAT
PflMI 1341 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4705 CCAGGGAGTGG
SacII 619 CCGCGG CCGC^GG
2781 CCGCGG
ScaI 565 AGTACT AGT^ACT
1445 AGTACT
SexAI 4275 ACCTGGT A^CC[AT]GGT
4340 ACCTGGT
SfoI 775 GGCGCC GGC^GCC
4965 GGCGCC
SspBI 5159 TGTACA T^GTACA
5434 TGTACA
TaqI 3998 TCGA T^CGA
5600 TCGA
TthHB8I 3998 TCGA T^CGA
5600 TCGA
Van91I 1341 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4705 CCAGGGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SphI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII XmaCI XmaI