################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AvaI AviII BbeI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsoBI BspDI BspEI BsrFI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI HgaI Hin1I HpaI KasI KpnI KspI MamI MluI MroI NaeI NarI NgoMIV NotI NruI NsiI PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfoI SgrAI SmaI SnaBI SphI Sse8387I SspI StuI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 3105 GTATAC GT^[AC][GT]AC AflII 4325 CTTAAG C^TTAAG Alw44I 3888 GTGCAC G^TGCAC AocI 3893 CCTGAGG CC^T[ACGT]AGG ApaI 1494 GGGCCC GGGCC^C ApaLI 3888 GTGCAC G^TGCAC AspI 800 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 658 CCTAGG C^CTAGG BbsI 4059 GTCTTC GAAGAC(2/6) BclI 4116 TGATCA T^GATCA BfrI 4325 CTTAAG C^TTAAG BlnI 658 CCTAGG C^CTAGG BpuAI 4059 GTCTTC GAAGAC(2/6) BsmBI 1107 GAGACG CGTCTC(1/5) Bsp1407I 3960 TGTACA T^GTACA BspHI 673 TCATGA T^CATGA BspLU11I 751 ACATGT A^CATGT BsrBI 2512 CCGCTC CCGCTC(-3/-3) BsrDI 1689 CATTGC GCAATG(2/0) BsrGI 3960 TGTACA T^GTACA BssHII 29 GCGCGC G^CGCGC Bst1107I 3105 GTATAC GTA^TAC BstBI 3593 TTCGAA TT^CGAA BstZ17I 3105 GTATAC GTA^TAC Bsu36I 3893 CCTGAGG CC^T[ACGT]AGG Eco81I 3893 CCTGAGG CC^T[ACGT]AGG EcoRI 2599 GAATTC G^AATTC FbaI 4116 TGATCA T^GATCA HindIII 139 AAGCTT A^AGCTT MfeI 1414 CAATTG C^AATTG MunI 1414 CAATTG C^AATTG NdeI 1380 CATATG CA^TATG NspV 3593 TTCGAA TT^CGAA PflFI 800 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 209 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 673 TCATGA T^CATGA ScaI 506 AGTACT AGT^ACT SexAI 1192 ACCAGGT A^CC[AT]GGT SfiI 1486 GGCCAGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 3593 TTCGAA TT^CGAA SspBI 3960 TGTACA T^GTACA Tth111I 800 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 1806 AAATTC [GA]^AATT[CT] 2599 GAATTC AlwNI 831 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG 2943 CAGATCCTG ApoI 1806 AAATTC [GA]^AATT[CT] 2599 GAATTC Asp700I 2432 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3982 GAATCCCTTC BanI 1112 GGCACC G^G[CT][GA]CC 2695 GGTGCC BbrPI 2092 CACGTG CAC^GTG 3021 CACGTG BcgI 3880 CGAATACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 856 GCAGCAAGATCG BglII 1405 AGATCT A^GATCT 2497 AGATCT BlpI 3825 GCTCAGC GC^T[ACGT]AGC 4350 GCTGAGC Bpu1102I 3825 GCTCAGC GC^T[ACGT]AGC 4350 GCTGAGC BsaAI 2092 CACGTG [CT]AC^GT[GA] 3021 CACGTG BsmI 3440 GAATGC GAATGC(1/-1) 1710 GCATTC BssSI 1901 CACGAG CACGAG(-5/-1) 4299 CTCGTG BstAPI 1684 GCAGTCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4312 GCACTGCTTGC CelII 3825 GCTCAGC GC^T[ACGT]AGC 4350 GCTGAGC EcoNI 739 CCTCAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3037 CCTGCAGCAGG HaeII 111 AGCGCC [GA]GCGC^[CT] 379 GGCGCT HincII 1274 GTCAAC GT[CT]^[GA]AC 2768 GTTGAC HindII 1274 GTCAAC GT[CT]^[GA]AC 2768 GTTGAC NheI 2507 GCTAGC G^CTAGC 4154 GCTAGC PmaCI 2092 CACGTG CAC^GTG 3021 CACGTG PmlI 2092 CACGTG CAC^GTG 3021 CACGTG SapI 2354 GAAGAGC GCTCTTC(1/4) 196 GCTCTTC SpeI 2903 ACTAGT A^CTAGT 3715 ACTAGT XbaI 356 TCTAGA T^CTAGA 4451 TCTAGA XmnI 2432 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3982 GAATCCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AspHI AvaII BalI BamHI BanII BbvCI BbvI BciVI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII