##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AvaI AviII
BbeI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsoBI BspDI BspEI
BsrFI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
FspI HgaI Hin1I HpaI KasI
KpnI KspI MamI MluI MroI
NaeI NarI NgoMIV NotI NruI
NsiI PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SfoI SgrAI SmaI
SnaBI SphI Sse8387I SspI StuI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 3105 GTATAC GT^[AC][GT]AC
AflII 4325 CTTAAG C^TTAAG
Alw44I 3888 GTGCAC G^TGCAC
AocI 3893 CCTGAGG CC^T[ACGT]AGG
ApaI 1494 GGGCCC GGGCC^C
ApaLI 3888 GTGCAC G^TGCAC
AspI 800 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 658 CCTAGG C^CTAGG
BbsI 4059 GTCTTC GAAGAC(2/6)
BclI 4116 TGATCA T^GATCA
BfrI 4325 CTTAAG C^TTAAG
BlnI 658 CCTAGG C^CTAGG
BpuAI 4059 GTCTTC GAAGAC(2/6)
BsmBI 1107 GAGACG CGTCTC(1/5)
Bsp1407I 3960 TGTACA T^GTACA
BspHI 673 TCATGA T^CATGA
BspLU11I 751 ACATGT A^CATGT
BsrBI 2512 CCGCTC CCGCTC(-3/-3)
BsrDI 1689 CATTGC GCAATG(2/0)
BsrGI 3960 TGTACA T^GTACA
BssHII 29 GCGCGC G^CGCGC
Bst1107I 3105 GTATAC GTA^TAC
BstBI 3593 TTCGAA TT^CGAA
BstZ17I 3105 GTATAC GTA^TAC
Bsu36I 3893 CCTGAGG CC^T[ACGT]AGG
Eco81I 3893 CCTGAGG CC^T[ACGT]AGG
EcoRI 2599 GAATTC G^AATTC
FbaI 4116 TGATCA T^GATCA
HindIII 139 AAGCTT A^AGCTT
MfeI 1414 CAATTG C^AATTG
MunI 1414 CAATTG C^AATTG
NdeI 1380 CATATG CA^TATG
NspV 3593 TTCGAA TT^CGAA
PflFI 800 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 209 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 673 TCATGA T^CATGA
ScaI 506 AGTACT AGT^ACT
SexAI 1192 ACCAGGT A^CC[AT]GGT
SfiI 1486 GGCCAGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3593 TTCGAA TT^CGAA
SspBI 3960 TGTACA T^GTACA
Tth111I 800 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 1806 AAATTC [GA]^AATT[CT]
2599 GAATTC
AlwNI 831 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2943 CAGATCCTG
ApoI 1806 AAATTC [GA]^AATT[CT]
2599 GAATTC
Asp700I 2432 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3982 GAATCCCTTC
BanI 1112 GGCACC G^G[CT][GA]CC
2695 GGTGCC
BbrPI 2092 CACGTG CAC^GTG
3021 CACGTG
BcgI 3880 CGAATACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
856 GCAGCAAGATCG
BglII 1405 AGATCT A^GATCT
2497 AGATCT
BlpI 3825 GCTCAGC GC^T[ACGT]AGC
4350 GCTGAGC
Bpu1102I 3825 GCTCAGC GC^T[ACGT]AGC
4350 GCTGAGC
BsaAI 2092 CACGTG [CT]AC^GT[GA]
3021 CACGTG
BsmI 3440 GAATGC GAATGC(1/-1)
1710 GCATTC
BssSI 1901 CACGAG CACGAG(-5/-1)
4299 CTCGTG
BstAPI 1684 GCAGTCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4312 GCACTGCTTGC
CelII 3825 GCTCAGC GC^T[ACGT]AGC
4350 GCTGAGC
EcoNI 739 CCTCAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3037 CCTGCAGCAGG
HaeII 111 AGCGCC [GA]GCGC^[CT]
379 GGCGCT
HincII 1274 GTCAAC GT[CT]^[GA]AC
2768 GTTGAC
HindII 1274 GTCAAC GT[CT]^[GA]AC
2768 GTTGAC
NheI 2507 GCTAGC G^CTAGC
4154 GCTAGC
PmaCI 2092 CACGTG CAC^GTG
3021 CACGTG
PmlI 2092 CACGTG CAC^GTG
3021 CACGTG
SapI 2354 GAAGAGC GCTCTTC(1/4)
196 GCTCTTC
SpeI 2903 ACTAGT A^CTAGT
3715 ACTAGT
XbaI 356 TCTAGA T^CTAGA
4451 TCTAGA
XmnI 2432 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3982 GAATCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AspHI AvaII BalI BamHI
BanII BbvCI BbvI BciVI BcnI
BfaI BglI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII