################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## Acc65I AccIII AclI AgeI AhdI AseI AsnI Asp718I AspEI AspI AviII BcgI BciVI BglII BsaBI BseAI BsiEI BsmI Bsp1407I BspDI BspEI BspHI BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FseI FspI KpnI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SfiI SfuI SmaI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 2485 GACGTC GACGT^C AflII 1568 CTTAAG C^TTAAG AflIII 2141 ACATGT A^C[GA][CT]GT Alw44I 388 GTGCAC G^TGCAC Aor51HI 2377 AGCGCT AGC^GCT ApaLI 388 GTGCAC G^TGCAC AscI 3609 GGCGCGCC GG^CGCGCC AvrII 3554 CCTAGG C^CTAGG BbeI 1276 GGCGCC GGCGC^C BbrPI 46 CACGTG CAC^GTG BbvCI 2686 CCTCAGC CCTCAGC(-5/-2) BclI 3328 TGATCA T^GATCA BfrI 1568 CTTAAG C^TTAAG BglI 1850 GCCGCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 3554 CCTAGG C^CTAGG BsaWI 3466 TCCGGT [AT]^CCGG[AT] BsiWI 248 CGTACG C^GTACG BspLU11I 2141 ACATGT A^CATGT BsrFI 392 ACCGGC [GA]^CCGG[CT] Cfr10I 392 ACCGGC [GA]^CCGG[CT] DraIII 2568 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1140 GACTGCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 2335 GAAGAG CTCTTC(1/4) Eco47III 2377 AGCGCT AGC^GCT FbaI 3328 TGATCA T^GATCA KasI 1276 GGCGCC G^GCGCC Ksp632I 2335 GAAGAG CTCTTC(1/4) KspI 2282 CCGCGG CCGC^GG NarI 1276 GGCGCC GG^CGCC NheI 4361 GCTAGC G^CTAGC PaeR7I 1162 CTCGAG C^TCGAG PflMI 3867 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 46 CACGTG CAC^GTG PmlI 46 CACGTG CAC^GTG PshAI 1248 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 2282 CCGCGG CCGC^GG SexAI 1378 ACCTGGT A^CC[AT]GGT SfoI 1276 GGCGCC GGC^GCC SgrAI 391 CACCGGCG C[GA]^CCGG[CT]G SspI 2877 AATATT AAT^ATT Van91I 3867 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1531 TCTAGA T^CTAGA XcmI 3867 CCAGATCCTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1162 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AccI 1469 GTAGAC GT^[AC][GT]AC 1492 GTAGAC Asp700I 558 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1064 GAACTACTTC BsaAI 46 CACGTG [CT]AC^GT[GA] 3770 TACGTG BsaI 1583 GAGACC GGTCTC(1/5) 4259 GAGACC BsmBI 978 GAGACG CGTCTC(1/5) 2535 GAGACG BsrDI 2606 GCAATG GCAATG(2/0) 3060 GCAATG BssHII 132 GCGCGC G^CGCGC 3610 GCGCGC BssSI 2000 CACGAG CACGAG(-5/-1) 264 CTCGTG EcoRI 65 GAATTC G^AATTC 2716 GAATTC HindIII 2204 AAGCTT A^AGCTT 2855 AAGCTT HpaI 3386 GTTAAC GTT^AAC 4152 GTTAAC NcoI 1500 CCATGG C^CATGG 4377 CCATGG SphI 167 GCATGC GCATG^C 3590 GCATGC TfiI 93 GAATC G^A[AT]TC 123 GAATC XmnI 558 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1064 GAACTACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII