##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
AseI AsnI Asp718I AspEI AspI
AviII BcgI BciVI BglII BsaBI
BseAI BsiEI BsmI Bsp1407I BspDI
BspEI BspHI BsrGI Bst1107I BstBI
BstZ17I ClaI CpoI DraI EagI
Eam1105I EclXI Eco52I EcoRV EcoT22I
FseI FspI KpnI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SfiI SfuI
SmaI SnaBI SpeI Sse8387I SspBI
SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2485 GACGTC GACGT^C
AflII 1568 CTTAAG C^TTAAG
AflIII 2141 ACATGT A^C[GA][CT]GT
Alw44I 388 GTGCAC G^TGCAC
Aor51HI 2377 AGCGCT AGC^GCT
ApaLI 388 GTGCAC G^TGCAC
AscI 3609 GGCGCGCC GG^CGCGCC
AvrII 3554 CCTAGG C^CTAGG
BbeI 1276 GGCGCC GGCGC^C
BbrPI 46 CACGTG CAC^GTG
BbvCI 2686 CCTCAGC CCTCAGC(-5/-2)
BclI 3328 TGATCA T^GATCA
BfrI 1568 CTTAAG C^TTAAG
BglI 1850 GCCGCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3554 CCTAGG C^CTAGG
BsaWI 3466 TCCGGT [AT]^CCGG[AT]
BsiWI 248 CGTACG C^GTACG
BspLU11I 2141 ACATGT A^CATGT
BsrFI 392 ACCGGC [GA]^CCGG[CT]
Cfr10I 392 ACCGGC [GA]^CCGG[CT]
DraIII 2568 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1140 GACTGCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2335 GAAGAG CTCTTC(1/4)
Eco47III 2377 AGCGCT AGC^GCT
FbaI 3328 TGATCA T^GATCA
KasI 1276 GGCGCC G^GCGCC
Ksp632I 2335 GAAGAG CTCTTC(1/4)
KspI 2282 CCGCGG CCGC^GG
NarI 1276 GGCGCC GG^CGCC
NheI 4361 GCTAGC G^CTAGC
PaeR7I 1162 CTCGAG C^TCGAG
PflMI 3867 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 46 CACGTG CAC^GTG
PmlI 46 CACGTG CAC^GTG
PshAI 1248 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 2282 CCGCGG CCGC^GG
SexAI 1378 ACCTGGT A^CC[AT]GGT
SfoI 1276 GGCGCC GGC^GCC
SgrAI 391 CACCGGCG C[GA]^CCGG[CT]G
SspI 2877 AATATT AAT^ATT
Van91I 3867 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1531 TCTAGA T^CTAGA
XcmI 3867 CCAGATCCTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1162 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 1469 GTAGAC GT^[AC][GT]AC
1492 GTAGAC
Asp700I 558 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1064 GAACTACTTC
BsaAI 46 CACGTG [CT]AC^GT[GA]
3770 TACGTG
BsaI 1583 GAGACC GGTCTC(1/5)
4259 GAGACC
BsmBI 978 GAGACG CGTCTC(1/5)
2535 GAGACG
BsrDI 2606 GCAATG GCAATG(2/0)
3060 GCAATG
BssHII 132 GCGCGC G^CGCGC
3610 GCGCGC
BssSI 2000 CACGAG CACGAG(-5/-1)
264 CTCGTG
EcoRI 65 GAATTC G^AATTC
2716 GAATTC
HindIII 2204 AAGCTT A^AGCTT
2855 AAGCTT
HpaI 3386 GTTAAC GTT^AAC
4152 GTTAAC
NcoI 1500 CCATGG C^CATGG
4377 CCATGG
SphI 167 GCATGC GCATG^C
3590 GCATGC
TfiI 93 GAATC G^A[AT]TC
123 GAATC
XmnI 558 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1064 GAACTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII