##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AflII AgeI AhdI Alw44I
Aor51HI ApaLI AspEI AvrII BamHI
BclI BfrI BglII BlnI BsaBI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI Eam1105I Eco47III EcoO65I
EcoRV EcoT22I FbaI FseI HincII
HindII HpaI MamI MfeI MunI
NdeI NotI NruI NsiI NspV
PinAI PmeI RsrII SalI ScaI
SexAI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 2191 TCCGGA T^CCGGA
AclI 245 AACGTT AA^CGTT
AcsI 3754 GAATTC [GA]^AATT[CT]
AflIII 3477 ACGCGT A^C[GA][CT]GT
ApoI 3754 GAATTC [GA]^AATT[CT]
AscI 2622 GGCGCGCC GG^CGCGCC
AseI 5003 ATTAAT AT^TAAT
AsnI 5003 ATTAAT AT^TAAT
Asp700I 3348 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1044 TGCGCA TGC^GCA
BbeI 1276 GGCGCC GGCGC^C
BbrPI 605 CACGTG CAC^GTG
BcgI 2712 CGAGTGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 605 CACGTG [CT]AC^GT[GA]
BseAI 2191 TCCGGA T^CCGGA
BspEI 2191 TCCGGA T^CCGGA
BspHI 4340 TCATGA T^CATGA
BssHII 2623 GCGCGC G^CGCGC
EcoRI 3754 GAATTC G^AATTC
FspI 1044 TGCGCA TGC^GCA
HindIII 4958 AAGCTT A^AGCTT
KasI 1276 GGCGCC G^GCGCC
KspI 462 CCGCGG CCGC^GG
MluI 3477 ACGCGT A^CGCGT
MroI 2191 TCCGGA T^CCGGA
NarI 1276 GGCGCC GG^CGCC
PacI 5004 TTAATTAA TTAAT^TAA
PmaCI 605 CACGTG CAC^GTG
PmlI 605 CACGTG CAC^GTG
PshBI 5003 ATTAAT AT^TAAT
Psp1406I 245 AACGTT AA^CGTT
PvuI 140 CGATCG CGAT^CG
RcaI 4340 TCATGA T^CATGA
SacII 462 CCGCGG CCGC^GG
SbfI 745 CCTGCAGG CCTGCA^GG
SfiI 3440 GGCCCCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1276 GGCGCC GGC^GCC
Sse8387I 745 CCTGCAGG CCTGCA^GG
SspI 4864 AATATT AAT^ATT
XmnI 3348 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 1688 GGTACC G^GTACC
3958 GGTACC
AccI 1873 GTCTAC GT^[AC][GT]AC
4771 GTCTAC
AcyI 1276 GGCGCC G[GA]^CG[CT]C
1626 GGCGTC
Asp718I 1688 GGTACC G^GTACC
3958 GGTACC
AspI 677 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2998 GACGGAGTC
BalI 2430 TGGCCA TGG^CCA
4207 TGGCCA
BbsI 2300 GTCTTC GAAGAC(2/6)
2988 GTCTTC
BciVI 3186 GGATAC GTATCC(6/5)
1799 GTATCC
BpuAI 2300 GTCTTC GAAGAC(2/6)
2988 GTCTTC
BsaHI 1276 GGCGCC G[GA]^CG[CT]C
1626 GGCGTC
BsaWI 2191 TCCGGA [AT]^CCGG[AT]
4148 TCCGGT
BspMI 2245 ACCTGC ACCTGC(4/8)
2008 GCAGGT
DraIII 992 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
4736 CACCGGGTG
EagI 17 CGGCCG C^GGCCG
1526 CGGCCG
EclXI 17 CGGCCG C^GGCCG
1526 CGGCCG
Eco52I 17 CGGCCG C^GGCCG
1526 CGGCCG
HgaI 677 GACGC GACGC(5/10)
1627 GCGTC
Hin1I 1276 GGCGCC G[GA]^CG[CT]C
1626 GGCGTC
KpnI 1688 GGTACC GGTAC^C
3958 GGTACC
MluNI 2430 TGGCCA TGG^CCA
4207 TGGCCA
MscI 2430 TGGCCA TGG^CCA
4207 TGGCCA
NaeI 52 GCCGGC GCC^GGC
773 GCCGGC
NcoI 364 CCATGG C^CATGG
4385 CCATGG
NgoMIV 52 GCCGGC G^CCGGC
773 GCCGGC
PaeR7I 1675 CTCGAG C^TCGAG
4356 CTCGAG
PflFI 677 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2998 GACGGAGTC
PpuMI 2262 AGGACCT [GA]G^G[AT]CC[CT]
3201 GGGACCC
Tth111I 677 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2998 GACGGAGTC
XbaI 3520 TCTAGA T^CTAGA
4255 TCTAGA
XhoI 1675 CTCGAG C^TCGAG
4356 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BanI BanII BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NheI NlaIII NlaIV NspI
PflMI PleI PshAI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII