################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AgeI Aor51HI AscI AspI AvaI AviII BbeI BbrPI BcgI BclI BfrI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI BstAPI BstUI Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoNI FbaI FseI FspI HaeII HgaI Hin1I KasI KspI MaeII MamI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SbfI SexAI SfoI SgrAI SmaI SnaBI Sse8387I SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 3844 GGTACC G^GTACC AhdI 3145 GACCCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1309 GTGCAC G^TGCAC ApaLI 1309 GTGCAC G^TGCAC AseI 2319 ATTAAT AT^TAAT AsnI 2319 ATTAAT AT^TAAT Asp718I 3844 GGTACC G^GTACC AspEI 3145 GACCCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 749 CCTAGG C^CTAGG BalI 2747 TGGCCA TGG^CCA BcnI 3206 CCGGG CC^[GC]GG BglII 3051 AGATCT A^GATCT BlnI 749 CCTAGG C^CTAGG BlpI 3006 GCTGAGC GC^T[ACGT]AGC BpmI 947 CTGGAG CTGGAG(16/14) Bpu1102I 3006 GCTGAGC GC^T[ACGT]AGC BsmI 2308 GAATGC GAATGC(1/-1) BspHI 3644 TCATGA T^CATGA BstBI 529 TTCGAA TT^CGAA BstEII 4336 GGTAACC G^GT[ACGT]ACC BstPI 4336 GGTAACC G^GT[ACGT]ACC CelII 3006 GCTGAGC GC^T[ACGT]AGC CfoI 3872 GCGC GCG^C DraI 4114 TTTAAA TTT^AAA Eam1105I 3145 GACCCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 4336 GGTAACC G^GT[ACGT]ACC EcoRI 4093 GAATTC G^AATTC HhaI 3872 GCGC GCG^C HinP1I 3872 GCGC G^CGC HpaI 3303 GTTAAC GTT^AAC KpnI 3844 GGTACC GGTAC^C MluNI 2747 TGGCCA TGG^CCA MscI 2747 TGGCCA TGG^CCA NciI 3206 CCGGG CC^[GC]GG NheI 3422 GCTAGC G^CTAGC NspV 529 TTCGAA TT^CGAA PshBI 2319 ATTAAT AT^TAAT PvuII 2245 CAGCTG CAG^CTG RcaI 3644 TCATGA T^CATGA SacI 1712 GAGCTC GAGCT^C SalI 2944 GTCGAC G^TCGAC SfiI 2106 GGCCTGGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 529 TTCGAA TT^CGAA SpeI 3999 ACTAGT A^CTAGT StuI 1528 AGGCCT AGG^CCT XbaI 2975 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AocI 341 CCTAAGG CC^T[ACGT]AGG 3374 CCTCAGG ApaI 2028 GGGCCC GGGCC^C 2114 GGGCCC Asp700I 1755 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2181 GAATTTTTTC BamHI 2042 GGATCC G^GATCC 3110 GGATCC BbvCI 332 GCTGAGG CCTCAGC(-5/-2) 768 GCTGAGG BciVI 1396 GTATCC GTATCC(6/5) 3972 GTATCC BsaI 3621 GAGACC GGTCTC(1/5) 1047 GGTCTC BspMI 2341 ACCTGC ACCTGC(4/8) 2386 ACCTGC Bst1107I 3390 GTATAC GTA^TAC 4157 GTATAC BstZ17I 3390 GTATAC GTA^TAC 4157 GTATAC Bsu36I 341 CCTAAGG CC^T[ACGT]AGG 3374 CCTCAGG Eco81I 341 CCTAAGG CC^T[ACGT]AGG 3374 CCTCAGG EcoRV 2910 GATATC GAT^ATC 3945 GATATC FauI 55 CCCGC CCCGC(4/6) 2992 GCGGG HapII 3191 CCGG C^CGG 3206 CCGG HpaII 3191 CCGG C^CGG 3206 CCGG MspA1I 168 CCGCTG C[AC]G^C[GT]G 2245 CAGCTG MspI 3191 CCGG C^CGG 3206 CCGG PshAI 62 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1629 GACTATGGTC SapI 89 GAAGAGC GCTCTTC(1/4) 1709 GAAGAGC XcmI 1508 CCACTTGAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3602 CCATGTTCATGCTGG XmnI 1755 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2181 GAATTTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbsI BbvI BfaI BglI BmyI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HincII HindII HindIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NcoI NdeI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SphI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII