##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI Aor51HI AscI AspI
AvaI AviII BbeI BbrPI BcgI
BclI BfrI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BsrBI BsrFI
BssHII BssSI BstAPI BstUI Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoNI FbaI
FseI FspI HaeII HgaI Hin1I
KasI KspI MaeII MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NgoMIV NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SbfI SexAI SfoI SgrAI SmaI
SnaBI Sse8387I SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 3844 GGTACC G^GTACC
AhdI 3145 GACCCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1309 GTGCAC G^TGCAC
ApaLI 1309 GTGCAC G^TGCAC
AseI 2319 ATTAAT AT^TAAT
AsnI 2319 ATTAAT AT^TAAT
Asp718I 3844 GGTACC G^GTACC
AspEI 3145 GACCCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 749 CCTAGG C^CTAGG
BalI 2747 TGGCCA TGG^CCA
BcnI 3206 CCGGG CC^[GC]GG
BglII 3051 AGATCT A^GATCT
BlnI 749 CCTAGG C^CTAGG
BlpI 3006 GCTGAGC GC^T[ACGT]AGC
BpmI 947 CTGGAG CTGGAG(16/14)
Bpu1102I 3006 GCTGAGC GC^T[ACGT]AGC
BsmI 2308 GAATGC GAATGC(1/-1)
BspHI 3644 TCATGA T^CATGA
BstBI 529 TTCGAA TT^CGAA
BstEII 4336 GGTAACC G^GT[ACGT]ACC
BstPI 4336 GGTAACC G^GT[ACGT]ACC
CelII 3006 GCTGAGC GC^T[ACGT]AGC
CfoI 3872 GCGC GCG^C
DraI 4114 TTTAAA TTT^AAA
Eam1105I 3145 GACCCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4336 GGTAACC G^GT[ACGT]ACC
EcoRI 4093 GAATTC G^AATTC
HhaI 3872 GCGC GCG^C
HinP1I 3872 GCGC G^CGC
HpaI 3303 GTTAAC GTT^AAC
KpnI 3844 GGTACC GGTAC^C
MluNI 2747 TGGCCA TGG^CCA
MscI 2747 TGGCCA TGG^CCA
NciI 3206 CCGGG CC^[GC]GG
NheI 3422 GCTAGC G^CTAGC
NspV 529 TTCGAA TT^CGAA
PshBI 2319 ATTAAT AT^TAAT
PvuII 2245 CAGCTG CAG^CTG
RcaI 3644 TCATGA T^CATGA
SacI 1712 GAGCTC GAGCT^C
SalI 2944 GTCGAC G^TCGAC
SfiI 2106 GGCCTGGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 529 TTCGAA TT^CGAA
SpeI 3999 ACTAGT A^CTAGT
StuI 1528 AGGCCT AGG^CCT
XbaI 2975 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AocI 341 CCTAAGG CC^T[ACGT]AGG
3374 CCTCAGG
ApaI 2028 GGGCCC GGGCC^C
2114 GGGCCC
Asp700I 1755 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2181 GAATTTTTTC
BamHI 2042 GGATCC G^GATCC
3110 GGATCC
BbvCI 332 GCTGAGG CCTCAGC(-5/-2)
768 GCTGAGG
BciVI 1396 GTATCC GTATCC(6/5)
3972 GTATCC
BsaI 3621 GAGACC GGTCTC(1/5)
1047 GGTCTC
BspMI 2341 ACCTGC ACCTGC(4/8)
2386 ACCTGC
Bst1107I 3390 GTATAC GTA^TAC
4157 GTATAC
BstZ17I 3390 GTATAC GTA^TAC
4157 GTATAC
Bsu36I 341 CCTAAGG CC^T[ACGT]AGG
3374 CCTCAGG
Eco81I 341 CCTAAGG CC^T[ACGT]AGG
3374 CCTCAGG
EcoRV 2910 GATATC GAT^ATC
3945 GATATC
FauI 55 CCCGC CCCGC(4/6)
2992 GCGGG
HapII 3191 CCGG C^CGG
3206 CCGG
HpaII 3191 CCGG C^CGG
3206 CCGG
MspA1I 168 CCGCTG C[AC]G^C[GT]G
2245 CAGCTG
MspI 3191 CCGG C^CGG
3206 CCGG
PshAI 62 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1629 GACTATGGTC
SapI 89 GAAGAGC GCTCTTC(1/4)
1709 GAAGAGC
XcmI 1508 CCACTTGAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3602 CCATGTTCATGCTGG
XmnI 1755 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2181 GAATTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BanI BanII BbsI BbvI
BfaI BglI BmyI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HincII HindII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NcoI NdeI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SphI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII