################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI Alw44I Aor51HI ApaLI AscI Asp718I AspI AviII AvrII BbrPI BcgI BclI BglII BlnI BsaAI BsaBI BsaI BseAI BsiEI BsiWI BspDI BspEI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FbaI FseI FspI HpaI KpnI KspI MamI MfeI MluI MroI MunI NaeI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 276 CGCG CG^CG AcyI 1784 GGCGCC G[GA]^CG[CT]C AflII 672 CTTAAG C^TTAAG AflIII 3904 ACATGT A^C[GA][CT]GT AhdI 2076 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 2213 GGGCCC GGGCC^C AseI 1280 ATTAAT AT^TAAT AsnI 1280 ATTAAT AT^TAAT AspEI 2076 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 611 TGGCCA TGG^CCA BamHI 238 GGATCC G^GATCC BbeI 1784 GGCGCC GGCGC^C BciVI 2041 GTATCC GTATCC(6/5) BcnI 1243 CCGGG CC^[GC]GG BfrI 672 CTTAAG C^TTAAG BsaHI 1784 GGCGCC G[GA]^CG[CT]C BsmBI 1510 CGTCTC CGTCTC(1/5) BspLU11I 3904 ACATGT A^CATGT BsrBI 2501 CCGCTC CCGCTC(-3/-3) BstUI 276 CGCG CG^CG DraIII 1218 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 611 TGGCCA [CT]^GGCC[GA] Eam1105I 2076 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 2436 ATGCAT ATGCA^T HaeII 1784 GGCGCC [GA]GCGC^[CT] Hin1I 1784 GGCGCC G[GA]^CG[CT]C HincII 3124 GTTGAC GT[CT]^[GA]AC HindII 3124 GTTGAC GT[CT]^[GA]AC KasI 1784 GGCGCC G^GCGCC MaeII 3174 ACGT A^CGT MluNI 611 TGGCCA TGG^CCA MscI 611 TGGCCA TGG^CCA MvnI 276 CGCG CG^CG NarI 1784 GGCGCC GG^CGCC NciI 1243 CCGGG CC^[GC]GG NcoI 3494 CCATGG C^CATGG NdeI 2433 CATATG CA^TATG NsiI 2436 ATGCAT ATGCA^T PaeR7I 765 CTCGAG C^TCGAG PflMI 3077 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 3258 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 2740 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1280 ATTAAT AT^TAAT PvuII 2427 CAGCTG CAG^CTG SacI 3239 GAGCTC GAGCT^C SapI 1614 GAAGAGC GCTCTTC(1/4) ScaI 2920 AGTACT AGT^ACT SexAI 327 ACCTGGT A^CC[AT]GGT SfiI 1712 GGCCCAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1784 GGCGCC GGC^GCC StuI 3324 AGGCCT AGG^CCT Van91I 3077 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 765 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwNI 364 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 2468 CAGCAACTG AocI 242 CCTGAGG CC^T[ACGT]AGG 3548 CCTAAGG Asp700I 380 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2088 GAATTCATTC AvaI 765 CTCGAG C^[CT]CG[GA]G 3405 CTCGGG BsmI 3473 GAATGC GAATGC(1/-1) 2438 GCATTC BsoBI 765 CTCGAG C^[CT]CG[GA]G 3405 CTCGGG BsrDI 471 GCAATG GCAATG(2/0) 3795 GCAATG BstXI 588 CCAGTAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 909 CCAGTCGGCTGG BstYI 131 AGATCC [GA]^GATC[CT] 238 GGATCC Bsu36I 242 CCTGAGG CC^T[ACGT]AGG 3548 CCTAAGG CfoI 1785 GCGC GCG^C 2000 GCGC DsaI 1222 CCGTGG C^C[GA][CT]GG 3494 CCATGG Eco81I 242 CCTGAGG CC^T[ACGT]AGG 3548 CCTAAGG FauI 1100 CCCGC CCCGC(4/6) 277 GCGGG HhaI 1785 GCGC GCG^C 2000 GCGC HinP1I 1785 GCGC G^CGC 2000 GCGC HindIII 2019 AAGCTT A^AGCTT 3838 AAGCTT MflI 131 AGATCC [GA]^GATC[CT] 238 GGATCC MspA1I 1101 CCGCTG C[AC]G^C[GT]G 2427 CAGCTG XhoII 131 AGATCC [GA]^GATC[CT] 238 GGATCC XmnI 380 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2088 GAATTCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanI BanII BbsI BbvCI BbvI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspHI BspMI BsrGI BsrI BssKI BstAPI BstF5I BstNI Cac8I CelII Cfr13I DdeI DpnII DraI DraII EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I