##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI Alw44I Aor51HI ApaLI AscI
Asp718I AspI AviII AvrII BbrPI
BcgI BclI BglII BlnI BsaAI
BsaBI BsaI BseAI BsiEI BsiWI
BspDI BspEI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
FbaI FseI FspI HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NgoMIV NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I XbaI XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccII 276 CGCG CG^CG
AcyI 1784 GGCGCC G[GA]^CG[CT]C
AflII 672 CTTAAG C^TTAAG
AflIII 3904 ACATGT A^C[GA][CT]GT
AhdI 2076 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2213 GGGCCC GGGCC^C
AseI 1280 ATTAAT AT^TAAT
AsnI 1280 ATTAAT AT^TAAT
AspEI 2076 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 611 TGGCCA TGG^CCA
BamHI 238 GGATCC G^GATCC
BbeI 1784 GGCGCC GGCGC^C
BciVI 2041 GTATCC GTATCC(6/5)
BcnI 1243 CCGGG CC^[GC]GG
BfrI 672 CTTAAG C^TTAAG
BsaHI 1784 GGCGCC G[GA]^CG[CT]C
BsmBI 1510 CGTCTC CGTCTC(1/5)
BspLU11I 3904 ACATGT A^CATGT
BsrBI 2501 CCGCTC CCGCTC(-3/-3)
BstUI 276 CGCG CG^CG
DraIII 1218 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 611 TGGCCA [CT]^GGCC[GA]
Eam1105I 2076 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 2436 ATGCAT ATGCA^T
HaeII 1784 GGCGCC [GA]GCGC^[CT]
Hin1I 1784 GGCGCC G[GA]^CG[CT]C
HincII 3124 GTTGAC GT[CT]^[GA]AC
HindII 3124 GTTGAC GT[CT]^[GA]AC
KasI 1784 GGCGCC G^GCGCC
MaeII 3174 ACGT A^CGT
MluNI 611 TGGCCA TGG^CCA
MscI 611 TGGCCA TGG^CCA
MvnI 276 CGCG CG^CG
NarI 1784 GGCGCC GG^CGCC
NciI 1243 CCGGG CC^[GC]GG
NcoI 3494 CCATGG C^CATGG
NdeI 2433 CATATG CA^TATG
NsiI 2436 ATGCAT ATGCA^T
PaeR7I 765 CTCGAG C^TCGAG
PflMI 3077 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3258 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2740 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1280 ATTAAT AT^TAAT
PvuII 2427 CAGCTG CAG^CTG
SacI 3239 GAGCTC GAGCT^C
SapI 1614 GAAGAGC GCTCTTC(1/4)
ScaI 2920 AGTACT AGT^ACT
SexAI 327 ACCTGGT A^CC[AT]GGT
SfiI 1712 GGCCCAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1784 GGCGCC GGC^GCC
StuI 3324 AGGCCT AGG^CCT
Van91I 3077 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 765 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 364 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2468 CAGCAACTG
AocI 242 CCTGAGG CC^T[ACGT]AGG
3548 CCTAAGG
Asp700I 380 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2088 GAATTCATTC
AvaI 765 CTCGAG C^[CT]CG[GA]G
3405 CTCGGG
BsmI 3473 GAATGC GAATGC(1/-1)
2438 GCATTC
BsoBI 765 CTCGAG C^[CT]CG[GA]G
3405 CTCGGG
BsrDI 471 GCAATG GCAATG(2/0)
3795 GCAATG
BstXI 588 CCAGTAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
909 CCAGTCGGCTGG
BstYI 131 AGATCC [GA]^GATC[CT]
238 GGATCC
Bsu36I 242 CCTGAGG CC^T[ACGT]AGG
3548 CCTAAGG
CfoI 1785 GCGC GCG^C
2000 GCGC
DsaI 1222 CCGTGG C^C[GA][CT]GG
3494 CCATGG
Eco81I 242 CCTGAGG CC^T[ACGT]AGG
3548 CCTAAGG
FauI 1100 CCCGC CCCGC(4/6)
277 GCGGG
HhaI 1785 GCGC GCG^C
2000 GCGC
HinP1I 1785 GCGC G^CGC
2000 GCGC
HindIII 2019 AAGCTT A^AGCTT
3838 AAGCTT
MflI 131 AGATCC [GA]^GATC[CT]
238 GGATCC
MspA1I 1101 CCGCTG C[AC]G^C[GT]G
2427 CAGCTG
XhoII 131 AGATCC [GA]^GATC[CT]
238 GGATCC
XmnI 380 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2088 GAATTCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanI BanII
BbsI BbvCI BbvI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspHI BspMI BsrGI BsrI
BssKI BstAPI BstF5I BstNI Cac8I
CelII Cfr13I DdeI DpnII DraI
DraII EarI EcoNI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I