################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## Acc65I AccIII AclI AgeI AscI AseI AsnI Asp700I Asp718I AspI AvrII BbeI BcnI BlnI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrFI BssHII BstAPI Cfr10I ClaI CpoI EagI EclXI Eco52I EcoRI EcoRV EcoT22I FseI KasI KpnI KspI MroI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 605 CGCG CG^CG AflII 2161 CTTAAG C^TTAAG AhdI 3784 GACATATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3691 GTGCAC G^TGCAC AlwNI 3613 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 4939 CCTTAGG CC^T[ACGT]AGG ApaLI 3691 GTGCAC G^TGCAC AspEI 3784 GACATATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 661 TGCGCA TGC^GCA BamHI 1361 GGATCC G^GATCC BbrPI 4953 CACGTG CAC^GTG BbsI 2507 GTCTTC GAAGAC(2/6) BbvCI 2844 CCTCAGC CCTCAGC(-5/-2) BcgI 288 CGAGCAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 4878 GGATAC GTATCC(6/5) BclI 4385 TGATCA T^GATCA BfrI 2161 CTTAAG C^TTAAG BglI 4063 GCCTGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 2507 GTCTTC GAAGAC(2/6) BsaWI 2351 ACCGGA [AT]^CCGG[AT] BsrBI 931 GAGCGG CCGCTC(-3/-3) BssSI 4799 CACGAG CACGAG(-5/-1) Bst1107I 3953 GTATAC GTA^TAC BstBI 231 TTCGAA TT^CGAA BstUI 605 CGCG CG^CG BstZ17I 3953 GTATAC GTA^TAC Bsu36I 4939 CCTTAGG CC^T[ACGT]AGG DraIII 727 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1385 GACTCCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 3784 GACATATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 4939 CCTTAGG CC^T[ACGT]AGG FbaI 4385 TGATCA T^GATCA FspI 661 TGCGCA TGC^GCA HpaI 4654 GTTAAC GTT^AAC MfeI 1697 CAATTG C^AATTG MluI 604 ACGCGT A^CGCGT MunI 1697 CAATTG C^AATTG MvnI 605 CGCG CG^CG NspV 231 TTCGAA TT^CGAA PmaCI 4953 CACGTG CAC^GTG PmlI 4953 CACGTG CAC^GTG PshAI 3433 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 2133 GAGCTC GAGCT^C SapI 3058 GCTCTTC GCTCTTC(1/4) ScaI 419 AGTACT AGT^ACT SexAI 47 ACCTGGT A^CC[AT]GGT SfuI 231 TTCGAA TT^CGAA StuI 2088 AGGCCT AGG^CCT XbaI 4348 TCTAGA T^CTAGA XcmI 506 CCATCTACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AatII 463 GACGTC GACGT^C 700 GACGTC AccI 1147 GTAGAC GT^[AC][GT]AC 3953 GTATAC AcyI 463 GACGTC G[GA]^CG[CT]C 700 GACGTC Aor51HI 11 AGCGCT AGC^GCT 4812 AGCGCT AvaI 286 CCCGAG C^[CT]CG[GA]G 392 CCCGAG BanI 3489 GGTGCC G^G[CT][GA]CC 3723 GGCACC BsaAI 949 CACGTA [CT]AC^GT[GA] 4953 CACGTG BsaBI 1091 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3205 GATGGCCATC BsaHI 463 GACGTC G[GA]^CG[CT]C 700 GACGTC BsmI 1704 GAATGC GAATGC(1/-1) 3461 GCATTC BsoBI 286 CCCGAG C^[CT]CG[GA]G 392 CCCGAG Bsp1407I 4217 TGTACA T^GTACA 5374 TGTACA BspHI 1087 TCATGA T^CATGA 2662 TCATGA BspLU11I 3305 ACATGT A^CATGT 3987 ACATGT BsrDI 3117 GCAATG GCAATG(2/0) 4865 GCAATG BsrGI 4217 TGTACA T^GTACA 5374 TGTACA BstEII 1297 GGTCACC G^GT[ACGT]ACC 1768 GGTCACC BstPI 1297 GGTCACC G^GT[ACGT]ACC 1768 GGTCACC BstXI 713 CCACAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1649 CCACAAACGTGG Eco47III 11 AGCGCT AGC^GCT 4812 AGCGCT EcoO65I 1297 GGTCACC G^GT[ACGT]ACC 1768 GGTCACC HaeII 11 AGCGCT [GA]GCGC^[CT] 4812 AGCGCT HgaI 444 GACGC GACGC(5/10) 606 GCGTC Hin1I 463 GACGTC G[GA]^CG[CT]C 700 GACGTC MamI 1091 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3205 GATGGCCATC NcoI 2477 CCATGG C^CATGG 4478 CCATGG PpuMI 2165 AGGACCT [GA]G^G[AT]CC[CT] 2271 AGGTCCT RcaI 1087 TCATGA T^CATGA 2662 TCATGA SpeI 1722 ACTAGT A^CTAGT 3190 ACTAGT SspBI 4217 TGTACA T^GTACA 5374 TGTACA SspI 3087 AATATT AAT^ATT 5297 AATATT TaqI 232 TCGA T^CGA 4925 TCGA TthHB8I 232 TCGA T^CGA 4925 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApaI ApoI AspHI AvaII BalI BanII BbvI BfaI BglII BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII