##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccIII AclI AgeI AscI
AseI AsnI Asp700I Asp718I AspI
AvrII BbeI BcnI BlnI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrFI BssHII BstAPI Cfr10I ClaI
CpoI EagI EclXI Eco52I EcoRI
EcoRV EcoT22I FseI KasI KpnI
KspI MroI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI Tth111I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 605 CGCG CG^CG
AflII 2161 CTTAAG C^TTAAG
AhdI 3784 GACATATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3691 GTGCAC G^TGCAC
AlwNI 3613 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 4939 CCTTAGG CC^T[ACGT]AGG
ApaLI 3691 GTGCAC G^TGCAC
AspEI 3784 GACATATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 661 TGCGCA TGC^GCA
BamHI 1361 GGATCC G^GATCC
BbrPI 4953 CACGTG CAC^GTG
BbsI 2507 GTCTTC GAAGAC(2/6)
BbvCI 2844 CCTCAGC CCTCAGC(-5/-2)
BcgI 288 CGAGCAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4878 GGATAC GTATCC(6/5)
BclI 4385 TGATCA T^GATCA
BfrI 2161 CTTAAG C^TTAAG
BglI 4063 GCCTGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 2507 GTCTTC GAAGAC(2/6)
BsaWI 2351 ACCGGA [AT]^CCGG[AT]
BsrBI 931 GAGCGG CCGCTC(-3/-3)
BssSI 4799 CACGAG CACGAG(-5/-1)
Bst1107I 3953 GTATAC GTA^TAC
BstBI 231 TTCGAA TT^CGAA
BstUI 605 CGCG CG^CG
BstZ17I 3953 GTATAC GTA^TAC
Bsu36I 4939 CCTTAGG CC^T[ACGT]AGG
DraIII 727 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1385 GACTCCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3784 GACATATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4939 CCTTAGG CC^T[ACGT]AGG
FbaI 4385 TGATCA T^GATCA
FspI 661 TGCGCA TGC^GCA
HpaI 4654 GTTAAC GTT^AAC
MfeI 1697 CAATTG C^AATTG
MluI 604 ACGCGT A^CGCGT
MunI 1697 CAATTG C^AATTG
MvnI 605 CGCG CG^CG
NspV 231 TTCGAA TT^CGAA
PmaCI 4953 CACGTG CAC^GTG
PmlI 4953 CACGTG CAC^GTG
PshAI 3433 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2133 GAGCTC GAGCT^C
SapI 3058 GCTCTTC GCTCTTC(1/4)
ScaI 419 AGTACT AGT^ACT
SexAI 47 ACCTGGT A^CC[AT]GGT
SfuI 231 TTCGAA TT^CGAA
StuI 2088 AGGCCT AGG^CCT
XbaI 4348 TCTAGA T^CTAGA
XcmI 506 CCATCTACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 463 GACGTC GACGT^C
700 GACGTC
AccI 1147 GTAGAC GT^[AC][GT]AC
3953 GTATAC
AcyI 463 GACGTC G[GA]^CG[CT]C
700 GACGTC
Aor51HI 11 AGCGCT AGC^GCT
4812 AGCGCT
AvaI 286 CCCGAG C^[CT]CG[GA]G
392 CCCGAG
BanI 3489 GGTGCC G^G[CT][GA]CC
3723 GGCACC
BsaAI 949 CACGTA [CT]AC^GT[GA]
4953 CACGTG
BsaBI 1091 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3205 GATGGCCATC
BsaHI 463 GACGTC G[GA]^CG[CT]C
700 GACGTC
BsmI 1704 GAATGC GAATGC(1/-1)
3461 GCATTC
BsoBI 286 CCCGAG C^[CT]CG[GA]G
392 CCCGAG
Bsp1407I 4217 TGTACA T^GTACA
5374 TGTACA
BspHI 1087 TCATGA T^CATGA
2662 TCATGA
BspLU11I 3305 ACATGT A^CATGT
3987 ACATGT
BsrDI 3117 GCAATG GCAATG(2/0)
4865 GCAATG
BsrGI 4217 TGTACA T^GTACA
5374 TGTACA
BstEII 1297 GGTCACC G^GT[ACGT]ACC
1768 GGTCACC
BstPI 1297 GGTCACC G^GT[ACGT]ACC
1768 GGTCACC
BstXI 713 CCACAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1649 CCACAAACGTGG
Eco47III 11 AGCGCT AGC^GCT
4812 AGCGCT
EcoO65I 1297 GGTCACC G^GT[ACGT]ACC
1768 GGTCACC
HaeII 11 AGCGCT [GA]GCGC^[CT]
4812 AGCGCT
HgaI 444 GACGC GACGC(5/10)
606 GCGTC
Hin1I 463 GACGTC G[GA]^CG[CT]C
700 GACGTC
MamI 1091 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3205 GATGGCCATC
NcoI 2477 CCATGG C^CATGG
4478 CCATGG
PpuMI 2165 AGGACCT [GA]G^G[AT]CC[CT]
2271 AGGTCCT
RcaI 1087 TCATGA T^CATGA
2662 TCATGA
SpeI 1722 ACTAGT A^CTAGT
3190 ACTAGT
SspBI 4217 TGTACA T^GTACA
5374 TGTACA
SspI 3087 AATATT AAT^ATT
5297 AATATT
TaqI 232 TCGA T^CGA
4925 TCGA
TthHB8I 232 TCGA T^CGA
4925 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApaI ApoI AspHI AvaII
BalI BanII BbvI BfaI BglII
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII