##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Aor51HI AscI Asp700I AviII BbrPI
BcgI BclI BfrI BlpI Bpu1102I
BsaBI BseAI BsiEI BsiWI BspDI
BspEI BsrFI BssHII BstBI BstEII
BstPI CelII Cfr10I ClaI CpoI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV FbaI FseI
FspI HpaI KspI MamI MfeI
MluI MroI MslI MunI NaeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI Van91I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 323 CGCG CG^CG
AcyI 1326 GGCGCC G[GA]^CG[CT]C
AhdI 3628 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3106 GTGCAC G^TGCAC
ApaI 1135 GGGCCC GGGCC^C
ApaLI 3106 GTGCAC G^TGCAC
AseI 3344 ATTAAT AT^TAAT
AsnI 3344 ATTAAT AT^TAAT
AspEI 3628 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2730 CCTAGG C^CTAGG
BalI 3488 TGGCCA TGG^CCA
BamHI 613 GGATCC G^GATCC
BbeI 1326 GGCGCC GGCGC^C
BciVI 711 GTATCC GTATCC(6/5)
BglI 3859 GCCTGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2730 CCTAGG C^CTAGG
BsaAI 4057 TACGTG [CT]AC^GT[GA]
BsaHI 1326 GGCGCC G[GA]^CG[CT]C
BsaWI 2770 ACCGGA [AT]^CCGG[AT]
BsmBI 1684 GAGACG CGTCTC(1/5)
BspHI 3293 TCATGA T^CATGA
BsrBI 181 GAGCGG CCGCTC(-3/-3)
BssSI 1381 CACGAG CACGAG(-5/-1)
BstUI 323 CGCG CG^CG
DraIII 2557 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3628 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 2827 ATGCAT ATGCA^T
FauI 1253 CCCGC CCCGC(4/6)
HaeII 1326 GGCGCC [GA]GCGC^[CT]
HgaI 321 GACGC GACGC(5/10)
Hin1I 1326 GGCGCC G[GA]^CG[CT]C
HincII 2475 GTCAAC GT[CT]^[GA]AC
HindII 2475 GTCAAC GT[CT]^[GA]AC
HindIII 1023 AAGCTT A^AGCTT
KasI 1326 GGCGCC G^GCGCC
MluNI 3488 TGGCCA TGG^CCA
MscI 3488 TGGCCA TGG^CCA
MvnI 323 CGCG CG^CG
NarI 1326 GGCGCC GG^CGCC
NdeI 961 CATATG CA^TATG
NsiI 2827 ATGCAT ATGCA^T
PpuMI 930 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 3344 ATTAAT AT^TAAT
RcaI 3293 TCATGA T^CATGA
SexAI 1278 ACCTGGT A^CC[AT]GGT
SfiI 3858 GGCCTGTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1326 GGCGCC GGC^GCC
SwaI 4006 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 2066 GGTACC G^GTACC
2767 GGTACC
AlwNI 1571 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
1871 CAGCCACTG
Asp718I 2066 GGTACC G^GTACC
2767 GGTACC
AspI 2574 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4073 GACTCAGTC
BglII 2182 AGATCT A^GATCT
2430 AGATCT
Bsp1407I 1819 TGTACA T^GTACA
3024 TGTACA
BspMI 2871 ACCTGC ACCTGC(4/8)
1293 GCAGGT
BsrGI 1819 TGTACA T^GTACA
3024 TGTACA
Bst1107I 156 GTATAC GTA^TAC
1459 GTATAC
BstAPI 847 GCACACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2829 GCATCCAGTGC
BstZ17I 156 GTATAC GTA^TAC
1459 GTATAC
CfoI 324 GCGC GCG^C
1327 GCGC
DrdI 1011 GACTAAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3668 GACGGTGCTGTC
EaeI 972 TGGCCG [CT]^GGCC[GA]
3488 TGGCCA
HhaI 324 GCGC GCG^C
1327 GCGC
HinP1I 324 GCGC G^CGC
1327 GCGC
KpnI 2066 GGTACC GGTAC^C
2767 GGTACC
MaeII 894 ACGT A^CGT
4058 ACGT
PflFI 2574 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4073 GACTCAGTC
SspBI 1819 TGTACA T^GTACA
3024 TGTACA
Tth111I 2574 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4073 GACTCAGTC
XcmI 997 CCACCAAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1331 CCAAGCCTGCATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AocI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspLU11I
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EarI Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII