##################################
# NO CUT ENZYMES (   49 enzymes) #
##################################

AatII       AccIII      AgeI        AhdI        AlwNI       
AscI        Asp700I     AspEI       AspI        AvrII       
BamHI       BciVI       BlnI        BsaBI       BseAI       
BsiWI       BspEI       Bst1107I    BstBI       BstZ17I     
CpoI        Eam1105I    EcoRI       EcoRV       FseI        
HpaI        MamI        MroI        NdeI        NotI        
NruI        NspV        PacI        PflFI       PinAI       
PmeI        PvuI        RsrII       SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SpeI        
Sse8387I    SwaI        Tth111I     XmnI        


###################################
# ONE CUT ENZYMES (   64 enzymes) #
###################################

Acc65I       1145  GGTACC               G^GTACC
Alw44I        152  GTGCAC               G^TGCAC
AocI         2359  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       496  AGCGCT               AGC^GCT
ApaLI         152  GTGCAC               G^TGCAC
AseI         3697  ATTAAT               AT^TAAT
AsnI         3697  ATTAAT               AT^TAAT
Asp718I      1145  GGTACC               G^GTACC
AviII         582  TGCGCA               TGC^GCA
BalI         1562  TGGCCA               TGG^CCA
BbrPI        3347  CACGTG               CAC^GTG
BclI         3951  TGATCA               T^GATCA
BlpI         4674  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     4674  GCTGAGC              GC^T[ACGT]AGC
Bsp1407I       42  TGTACA               T^GTACA
BspDI        2112  ATCGAT               AT^CGAT
BspHI        3110  TCATGA               T^CATGA
BspMI         907  ACCTGC               ACCTGC(4/8)
BsrBI         254  GAGCGG               CCGCTC(-3/-3)
BsrFI        3902  GCCGGC               [GA]^CCGG[CT]
BsrGI          42  TGTACA               T^GTACA
BssSI        4234  CTCGTG               CACGAG(-5/-1)
BstAPI       4620  GCAAGGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        2321  CCACGGCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       2359  CCTCAGG              CC^T[ACGT]AGG
CelII        4674  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       3902  GCCGGC               [GA]^CCGG[CT]
ClaI         2112  ATCGAT               AT^CGAT
DraI         3639  TTTAAA               TTT^AAA
DrdI         4467  GACCGAGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      496  AGCGCT               AGC^GCT
Eco81I       2359  CCTCAGG              CC^T[ACGT]AGG
EcoNI        3012  CCTAGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      2116  ATGCAT               ATGCA^T
FbaI         3951  TGATCA               T^GATCA
FspI          582  TGCGCA               TGC^GCA
HincII        811  GTCGAC               GT[CT]^[GA]AC
HindII        811  GTCGAC               GT[CT]^[GA]AC
KpnI         1145  GGTACC               GGTAC^C
KspI          445  CCGCGG               CCGC^GG
MluI         4069  ACGCGT               A^CGCGT
MluNI        1562  TGGCCA               TGG^CCA
MscI         1562  TGGCCA               TGG^CCA
NaeI         3902  GCCGGC               GCC^GGC
NgoMIV       3902  GCCGGC               G^CCGGC
NheI         4081  GCTAGC               G^CTAGC
NsiI         2116  ATGCAT               ATGCA^T
PaeR7I         15  CTCGAG               C^TCGAG
PmaCI        3347  CACGTG               CAC^GTG
PmlI         3347  CACGTG               CAC^GTG
PshAI        2238  GACCATGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3697  ATTAAT               AT^TAAT
RcaI         3110  TCATGA               T^CATGA
SacII         445  CCGCGG               CCGC^GG
SalI          811  GTCGAC               G^TCGAC
SapI           57  GCTCTTC              GCTCTTC(1/4)
SmaI          290  CCCGGG               CCC^GGG
SnaBI        4963  TACGTA               TAC^GTA
SphI         4181  GCATGC               GCATG^C
SspBI          42  TGTACA               T^GTACA
XbaI          678  TCTAGA               T^CTAGA
XhoI           15  CTCGAG               C^TCGAG
XmaCI         290  CCCGGG               C^CCGGG
XmaI          290  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI          811  GTCGAC               GT^[AC][GT]AC
             2208  GTCTAC              
AclI          596  AACGTT               AA^CGTT
             4272  AACGTT              
AcsI          139  AAATTT               [GA]^AATT[CT]
             3667  AAATTC              
AflII        1090  CTTAAG               C^TTAAG
             4478  CTTAAG              
ApaI            6  GGGCCC               GGGCC^C
             3102  GGGCCC              
ApoI          139  AAATTT               [GA]^AATT[CT]
             3667  AAATTC              
BcgI          959  CGAAAGATCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              467  GCAGCGGGCTCG        
BfrI         1090  CTTAAG               C^TTAAG
             4478  CTTAAG              
BglII         963  AGATCT               A^GATCT
             3405  AGATCT              
BsaI         1085  GAGACC               GGTCTC(1/5)
             4593  GAGACC              
BsaWI         283  TCCGGT               [AT]^CCGG[AT]
             4320  ACCGGA              
BsiEI         419  CGGCCG               CG[GA][CT]^CG
              451  CGGCCG              
BsmBI        1694  CGTCTC               CGTCTC(1/5)
             3504  CGTCTC              
BspLU11I     2381  ACATGT               A^CATGT
             4550  ACATGT              
BsrDI        3066  CATTGC               GCAATG(2/0)
             2414  GCAATG              
BstEII       2235  GGTGACC              G^GT[ACGT]ACC
             3458  GGTTACC             
BstPI        2235  GGTGACC              G^GT[ACGT]ACC
             3458  GGTTACC             
DraIII       3145  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3344  CACCACGTG           
EagI          419  CGGCCG               C^GGCCG
              451  CGGCCG              
EclXI         419  CGGCCG               C^GGCCG
              451  CGGCCG              
Eco52I        419  CGGCCG               C^GGCCG
              451  CGGCCG              
EcoO65I      2235  GGTGACC              G^GT[ACGT]ACC
             3458  GGTTACC             
HindIII        27  AAGCTT               A^AGCTT
             3331  AAGCTT              
MfeI         3603  CAATTG               C^AATTG
             4656  CAATTG              
MunI         3603  CAATTG               C^AATTG
             4656  CAATTG              
PflMI        1596  CCACTCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4054  CCATTGACTGG         
Psp1406I      596  AACGTT               AA^CGTT
             4272  AACGTT              
Van91I       1596  CCACTCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4054  CCATTGACTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AspHI       AvaI        AvaII       
BanI        BanII       BbeI        BbsI        BbvCI       
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaAI       BsaHI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BsrI        BssHII      BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NarI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PpuMI       PstI        PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfoI        SmlI        SspI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII