################################## # NO CUT ENZYMES ( 49 enzymes) # ################################## AatII AccIII AgeI AhdI AlwNI AscI Asp700I AspEI AspI AvrII BamHI BciVI BlnI BsaBI BseAI BsiWI BspEI Bst1107I BstBI BstZ17I CpoI Eam1105I EcoRI EcoRV FseI HpaI MamI MroI NdeI NotI NruI NspV PacI PflFI PinAI PmeI PvuI RsrII SbfI ScaI SexAI SfiI SfuI SgrAI SpeI Sse8387I SwaI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### Acc65I 1145 GGTACC G^GTACC Alw44I 152 GTGCAC G^TGCAC AocI 2359 CCTCAGG CC^T[ACGT]AGG Aor51HI 496 AGCGCT AGC^GCT ApaLI 152 GTGCAC G^TGCAC AseI 3697 ATTAAT AT^TAAT AsnI 3697 ATTAAT AT^TAAT Asp718I 1145 GGTACC G^GTACC AviII 582 TGCGCA TGC^GCA BalI 1562 TGGCCA TGG^CCA BbrPI 3347 CACGTG CAC^GTG BclI 3951 TGATCA T^GATCA BlpI 4674 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4674 GCTGAGC GC^T[ACGT]AGC Bsp1407I 42 TGTACA T^GTACA BspDI 2112 ATCGAT AT^CGAT BspHI 3110 TCATGA T^CATGA BspMI 907 ACCTGC ACCTGC(4/8) BsrBI 254 GAGCGG CCGCTC(-3/-3) BsrFI 3902 GCCGGC [GA]^CCGG[CT] BsrGI 42 TGTACA T^GTACA BssSI 4234 CTCGTG CACGAG(-5/-1) BstAPI 4620 GCAAGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2321 CCACGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2359 CCTCAGG CC^T[ACGT]AGG CelII 4674 GCTGAGC GC^T[ACGT]AGC Cfr10I 3902 GCCGGC [GA]^CCGG[CT] ClaI 2112 ATCGAT AT^CGAT DraI 3639 TTTAAA TTT^AAA DrdI 4467 GACCGAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 496 AGCGCT AGC^GCT Eco81I 2359 CCTCAGG CC^T[ACGT]AGG EcoNI 3012 CCTAGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 2116 ATGCAT ATGCA^T FbaI 3951 TGATCA T^GATCA FspI 582 TGCGCA TGC^GCA HincII 811 GTCGAC GT[CT]^[GA]AC HindII 811 GTCGAC GT[CT]^[GA]AC KpnI 1145 GGTACC GGTAC^C KspI 445 CCGCGG CCGC^GG MluI 4069 ACGCGT A^CGCGT MluNI 1562 TGGCCA TGG^CCA MscI 1562 TGGCCA TGG^CCA NaeI 3902 GCCGGC GCC^GGC NgoMIV 3902 GCCGGC G^CCGGC NheI 4081 GCTAGC G^CTAGC NsiI 2116 ATGCAT ATGCA^T PaeR7I 15 CTCGAG C^TCGAG PmaCI 3347 CACGTG CAC^GTG PmlI 3347 CACGTG CAC^GTG PshAI 2238 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3697 ATTAAT AT^TAAT RcaI 3110 TCATGA T^CATGA SacII 445 CCGCGG CCGC^GG SalI 811 GTCGAC G^TCGAC SapI 57 GCTCTTC GCTCTTC(1/4) SmaI 290 CCCGGG CCC^GGG SnaBI 4963 TACGTA TAC^GTA SphI 4181 GCATGC GCATG^C SspBI 42 TGTACA T^GTACA XbaI 678 TCTAGA T^CTAGA XhoI 15 CTCGAG C^TCGAG XmaCI 290 CCCGGG C^CCGGG XmaI 290 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 811 GTCGAC GT^[AC][GT]AC 2208 GTCTAC AclI 596 AACGTT AA^CGTT 4272 AACGTT AcsI 139 AAATTT [GA]^AATT[CT] 3667 AAATTC AflII 1090 CTTAAG C^TTAAG 4478 CTTAAG ApaI 6 GGGCCC GGGCC^C 3102 GGGCCC ApoI 139 AAATTT [GA]^AATT[CT] 3667 AAATTC BcgI 959 CGAAAGATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 467 GCAGCGGGCTCG BfrI 1090 CTTAAG C^TTAAG 4478 CTTAAG BglII 963 AGATCT A^GATCT 3405 AGATCT BsaI 1085 GAGACC GGTCTC(1/5) 4593 GAGACC BsaWI 283 TCCGGT [AT]^CCGG[AT] 4320 ACCGGA BsiEI 419 CGGCCG CG[GA][CT]^CG 451 CGGCCG BsmBI 1694 CGTCTC CGTCTC(1/5) 3504 CGTCTC BspLU11I 2381 ACATGT A^CATGT 4550 ACATGT BsrDI 3066 CATTGC GCAATG(2/0) 2414 GCAATG BstEII 2235 GGTGACC G^GT[ACGT]ACC 3458 GGTTACC BstPI 2235 GGTGACC G^GT[ACGT]ACC 3458 GGTTACC DraIII 3145 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG 3344 CACCACGTG EagI 419 CGGCCG C^GGCCG 451 CGGCCG EclXI 419 CGGCCG C^GGCCG 451 CGGCCG Eco52I 419 CGGCCG C^GGCCG 451 CGGCCG EcoO65I 2235 GGTGACC G^GT[ACGT]ACC 3458 GGTTACC HindIII 27 AAGCTT A^AGCTT 3331 AAGCTT MfeI 3603 CAATTG C^AATTG 4656 CAATTG MunI 3603 CAATTG C^AATTG 4656 CAATTG PflMI 1596 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4054 CCATTGACTGG Psp1406I 596 AACGTT AA^CGTT 4272 AACGTT Van91I 1596 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4054 CCATTGACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AspHI AvaI AvaII BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrI BssHII BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII