##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII AccIII AgeI AhdI AlwNI
AscI Asp700I AspEI AspI AvrII
BamHI BciVI BlnI BsaBI BseAI
BsiWI BspEI Bst1107I BstBI BstZ17I
CpoI Eam1105I EcoRI EcoRV FseI
HpaI MamI MroI NdeI NotI
NruI NspV PacI PflFI PinAI
PmeI PvuI RsrII SbfI ScaI
SexAI SfiI SfuI SgrAI SpeI
Sse8387I SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
Acc65I 1145 GGTACC G^GTACC
Alw44I 152 GTGCAC G^TGCAC
AocI 2359 CCTCAGG CC^T[ACGT]AGG
Aor51HI 496 AGCGCT AGC^GCT
ApaLI 152 GTGCAC G^TGCAC
AseI 3697 ATTAAT AT^TAAT
AsnI 3697 ATTAAT AT^TAAT
Asp718I 1145 GGTACC G^GTACC
AviII 582 TGCGCA TGC^GCA
BalI 1562 TGGCCA TGG^CCA
BbrPI 3347 CACGTG CAC^GTG
BclI 3951 TGATCA T^GATCA
BlpI 4674 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4674 GCTGAGC GC^T[ACGT]AGC
Bsp1407I 42 TGTACA T^GTACA
BspDI 2112 ATCGAT AT^CGAT
BspHI 3110 TCATGA T^CATGA
BspMI 907 ACCTGC ACCTGC(4/8)
BsrBI 254 GAGCGG CCGCTC(-3/-3)
BsrFI 3902 GCCGGC [GA]^CCGG[CT]
BsrGI 42 TGTACA T^GTACA
BssSI 4234 CTCGTG CACGAG(-5/-1)
BstAPI 4620 GCAAGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2321 CCACGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2359 CCTCAGG CC^T[ACGT]AGG
CelII 4674 GCTGAGC GC^T[ACGT]AGC
Cfr10I 3902 GCCGGC [GA]^CCGG[CT]
ClaI 2112 ATCGAT AT^CGAT
DraI 3639 TTTAAA TTT^AAA
DrdI 4467 GACCGAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 496 AGCGCT AGC^GCT
Eco81I 2359 CCTCAGG CC^T[ACGT]AGG
EcoNI 3012 CCTAGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2116 ATGCAT ATGCA^T
FbaI 3951 TGATCA T^GATCA
FspI 582 TGCGCA TGC^GCA
HincII 811 GTCGAC GT[CT]^[GA]AC
HindII 811 GTCGAC GT[CT]^[GA]AC
KpnI 1145 GGTACC GGTAC^C
KspI 445 CCGCGG CCGC^GG
MluI 4069 ACGCGT A^CGCGT
MluNI 1562 TGGCCA TGG^CCA
MscI 1562 TGGCCA TGG^CCA
NaeI 3902 GCCGGC GCC^GGC
NgoMIV 3902 GCCGGC G^CCGGC
NheI 4081 GCTAGC G^CTAGC
NsiI 2116 ATGCAT ATGCA^T
PaeR7I 15 CTCGAG C^TCGAG
PmaCI 3347 CACGTG CAC^GTG
PmlI 3347 CACGTG CAC^GTG
PshAI 2238 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3697 ATTAAT AT^TAAT
RcaI 3110 TCATGA T^CATGA
SacII 445 CCGCGG CCGC^GG
SalI 811 GTCGAC G^TCGAC
SapI 57 GCTCTTC GCTCTTC(1/4)
SmaI 290 CCCGGG CCC^GGG
SnaBI 4963 TACGTA TAC^GTA
SphI 4181 GCATGC GCATG^C
SspBI 42 TGTACA T^GTACA
XbaI 678 TCTAGA T^CTAGA
XhoI 15 CTCGAG C^TCGAG
XmaCI 290 CCCGGG C^CCGGG
XmaI 290 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 811 GTCGAC GT^[AC][GT]AC
2208 GTCTAC
AclI 596 AACGTT AA^CGTT
4272 AACGTT
AcsI 139 AAATTT [GA]^AATT[CT]
3667 AAATTC
AflII 1090 CTTAAG C^TTAAG
4478 CTTAAG
ApaI 6 GGGCCC GGGCC^C
3102 GGGCCC
ApoI 139 AAATTT [GA]^AATT[CT]
3667 AAATTC
BcgI 959 CGAAAGATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
467 GCAGCGGGCTCG
BfrI 1090 CTTAAG C^TTAAG
4478 CTTAAG
BglII 963 AGATCT A^GATCT
3405 AGATCT
BsaI 1085 GAGACC GGTCTC(1/5)
4593 GAGACC
BsaWI 283 TCCGGT [AT]^CCGG[AT]
4320 ACCGGA
BsiEI 419 CGGCCG CG[GA][CT]^CG
451 CGGCCG
BsmBI 1694 CGTCTC CGTCTC(1/5)
3504 CGTCTC
BspLU11I 2381 ACATGT A^CATGT
4550 ACATGT
BsrDI 3066 CATTGC GCAATG(2/0)
2414 GCAATG
BstEII 2235 GGTGACC G^GT[ACGT]ACC
3458 GGTTACC
BstPI 2235 GGTGACC G^GT[ACGT]ACC
3458 GGTTACC
DraIII 3145 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3344 CACCACGTG
EagI 419 CGGCCG C^GGCCG
451 CGGCCG
EclXI 419 CGGCCG C^GGCCG
451 CGGCCG
Eco52I 419 CGGCCG C^GGCCG
451 CGGCCG
EcoO65I 2235 GGTGACC G^GT[ACGT]ACC
3458 GGTTACC
HindIII 27 AAGCTT A^AGCTT
3331 AAGCTT
MfeI 3603 CAATTG C^AATTG
4656 CAATTG
MunI 3603 CAATTG C^AATTG
4656 CAATTG
PflMI 1596 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4054 CCATTGACTGG
Psp1406I 596 AACGTT AA^CGTT
4272 AACGTT
Van91I 1596 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4054 CCATTGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AspHI AvaI AvaII
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII