##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI BcgI BciVI BfrI BsaBI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DrdI EagI
EclXI Eco52I Eco81I EcoO65I HincII
HindII HpaI KspI MamI MfeI
MluI MunI NcoI NdeI NheI
NotI NruI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SphI SspBI SwaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 2244 GGTACC G^GTACC
AccI 695 GTCTAC GT^[AC][GT]AC
AccIII 2307 TCCGGA T^CCGGA
AcyI 2203 GGCGCC G[GA]^CG[CT]C
AhdI 2889 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2788 AGCGCT AGC^GCT
Asp700I 3890 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2244 GGTACC G^GTACC
AspEI 2889 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 207 TGCGCA TGC^GCA
AvrII 3311 CCTAGG C^CTAGG
BamHI 2397 GGATCC G^GATCC
BbeI 2203 GGCGCC GGCGC^C
BbvCI 144 CCTCAGC CCTCAGC(-5/-2)
BclI 624 TGATCA T^GATCA
BlnI 3311 CCTAGG C^CTAGG
BlpI 2039 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2039 GCTGAGC GC^T[ACGT]AGC
BsaHI 2203 GGCGCC G[GA]^CG[CT]C
BsaI 2887 GAGACC GGTCTC(1/5)
BseAI 2307 TCCGGA T^CCGGA
BsiEI 3212 CGGTCG CG[GA][CT]^CG
BspEI 2307 TCCGGA T^CCGGA
BspMI 2890 ACCTGC ACCTGC(4/8)
CelII 2039 GCTGAGC GC^T[ACGT]AGC
DraIII 852 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2889 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2788 AGCGCT AGC^GCT
EcoRV 2908 GATATC GAT^ATC
FbaI 624 TGATCA T^GATCA
FseI 2084 GGCCGGCC GGCCGG^CC
FspI 207 TGCGCA TGC^GCA
Hin1I 2203 GGCGCC G[GA]^CG[CT]C
KasI 2203 GGCGCC G^GCGCC
KpnI 2244 GGTACC GGTAC^C
MroI 2307 TCCGGA T^CCGGA
NarI 2203 GGCGCC GG^CGCC
PflMI 2527 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 1458 GCTCTTC GCTCTTC(1/4)
SbfI 2176 CCTGCAGG CCTGCA^GG
SfiI 217 GGCCTGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2203 GGCGCC GGC^GCC
SmaI 2823 CCCGGG CCC^GGG
Sse8387I 2176 CCTGCAGG CCTGCA^GG
StuI 1180 AGGCCT AGG^CCT
Van91I 2527 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3886 TCTAGA T^CTAGA
XmaCI 2823 CCCGGG C^CCGGG
XmaI 2823 CCCGGG C^CCGGG
XmnI 3890 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 3286 ACACGT A^C[GA][CT]GT
3417 ACGTGT
AlwNI 1524 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
3769 CAGGAGCTG
AspI 389 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
749 GACTCAGTC
BglI 161 GCCCCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
218 GCCTGGAGGGC
BsaWI 2307 TCCGGA [AT]^CCGG[AT]
3354 ACCGGA
BspHI 1333 TCATGA T^CATGA
3463 TCATGA
BstAPI 1523 GCAGAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1823 GCAGAGTTTGC
DraI 3628 TTTAAA TTT^AAA
4057 TTTAAA
DsaI 165 CCACGG C^C[GA][CT]GG
1298 CCACGG
EcoNI 397 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1134 CCTCGAGCAGG
EcoRI 862 GAATTC G^AATTC
3955 GAATTC
EcoT22I 542 ATGCAT ATGCA^T
1066 ATGCAT
HindIII 1888 AAGCTT A^AGCTT
3904 AAGCTT
NsiI 542 ATGCAT ATGCA^T
1066 ATGCAT
NspI 592 ACATGC [GA]CATG^[CT]
1068 GCATGT
PaeR7I 1135 CTCGAG C^TCGAG
1163 CTCGAG
PflFI 389 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
749 GACTCAGTC
RcaI 1333 TCATGA T^CATGA
3463 TCATGA
SspI 2798 AATATT AAT^ATT
3891 AATATT
Tth111I 389 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
749 GACTCAGTC
XhoI 1135 CTCGAG C^TCGAG
1163 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII