################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII AclI AflII AgeI Alw44I AocI ApaI ApaLI AscI AseI AsnI BcgI BciVI BfrI BsaBI BsiWI BsmBI BsmI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DrdI EagI EclXI Eco52I Eco81I EcoO65I HincII HindII HpaI KspI MamI MfeI MluI MunI NcoI NdeI NheI NotI NruI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI SspBI SwaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 2244 GGTACC G^GTACC AccI 695 GTCTAC GT^[AC][GT]AC AccIII 2307 TCCGGA T^CCGGA AcyI 2203 GGCGCC G[GA]^CG[CT]C AhdI 2889 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 2788 AGCGCT AGC^GCT Asp700I 3890 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2244 GGTACC G^GTACC AspEI 2889 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 207 TGCGCA TGC^GCA AvrII 3311 CCTAGG C^CTAGG BamHI 2397 GGATCC G^GATCC BbeI 2203 GGCGCC GGCGC^C BbvCI 144 CCTCAGC CCTCAGC(-5/-2) BclI 624 TGATCA T^GATCA BlnI 3311 CCTAGG C^CTAGG BlpI 2039 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2039 GCTGAGC GC^T[ACGT]AGC BsaHI 2203 GGCGCC G[GA]^CG[CT]C BsaI 2887 GAGACC GGTCTC(1/5) BseAI 2307 TCCGGA T^CCGGA BsiEI 3212 CGGTCG CG[GA][CT]^CG BspEI 2307 TCCGGA T^CCGGA BspMI 2890 ACCTGC ACCTGC(4/8) CelII 2039 GCTGAGC GC^T[ACGT]AGC DraIII 852 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 2889 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2788 AGCGCT AGC^GCT EcoRV 2908 GATATC GAT^ATC FbaI 624 TGATCA T^GATCA FseI 2084 GGCCGGCC GGCCGG^CC FspI 207 TGCGCA TGC^GCA Hin1I 2203 GGCGCC G[GA]^CG[CT]C KasI 2203 GGCGCC G^GCGCC KpnI 2244 GGTACC GGTAC^C MroI 2307 TCCGGA T^CCGGA NarI 2203 GGCGCC GG^CGCC PflMI 2527 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SapI 1458 GCTCTTC GCTCTTC(1/4) SbfI 2176 CCTGCAGG CCTGCA^GG SfiI 217 GGCCTGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 2203 GGCGCC GGC^GCC SmaI 2823 CCCGGG CCC^GGG Sse8387I 2176 CCTGCAGG CCTGCA^GG StuI 1180 AGGCCT AGG^CCT Van91I 2527 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3886 TCTAGA T^CTAGA XmaCI 2823 CCCGGG C^CCGGG XmaI 2823 CCCGGG C^CCGGG XmnI 3890 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AflIII 3286 ACACGT A^C[GA][CT]GT 3417 ACGTGT AlwNI 1524 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG 3769 CAGGAGCTG AspI 389 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 749 GACTCAGTC BglI 161 GCCCCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 218 GCCTGGAGGGC BsaWI 2307 TCCGGA [AT]^CCGG[AT] 3354 ACCGGA BspHI 1333 TCATGA T^CATGA 3463 TCATGA BstAPI 1523 GCAGAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1823 GCAGAGTTTGC DraI 3628 TTTAAA TTT^AAA 4057 TTTAAA DsaI 165 CCACGG C^C[GA][CT]GG 1298 CCACGG EcoNI 397 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1134 CCTCGAGCAGG EcoRI 862 GAATTC G^AATTC 3955 GAATTC EcoT22I 542 ATGCAT ATGCA^T 1066 ATGCAT HindIII 1888 AAGCTT A^AGCTT 3904 AAGCTT NsiI 542 ATGCAT ATGCA^T 1066 ATGCAT NspI 592 ACATGC [GA]CATG^[CT] 1068 GCATGT PaeR7I 1135 CTCGAG C^TCGAG 1163 CTCGAG PflFI 389 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 749 GACTCAGTC RcaI 1333 TCATGA T^CATGA 3463 TCATGA SspI 2798 AATATT AAT^ATT 3891 AATATT Tth111I 389 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 749 GACTCAGTC XhoI 1135 CTCGAG C^TCGAG 1163 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII