##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AclI AgeI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AvaI BamHI BsiEI
BsiWI BsoBI BspDI BsrBI BsrFI
BssHII BssSI BstBI BstEII BstPI
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI HgaI HincII HindII HpaI
KpnI KspI MfeI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccIII 960 TCCGGA T^CCGGA
AcyI 1959 GGCGCC G[GA]^CG[CT]C
AocI 2816 CCTGAGG CC^T[ACGT]AGG
Asp700I 2105 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3711 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1106 TGCGCA TGC^GCA
BbeI 1959 GGCGCC GGCGC^C
BbrPI 25 CACGTG CAC^GTG
BbvCI 712 GCTGAGG CCTCAGC(-5/-2)
BcgI 1626 CGAGAACCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1463 GTATCC GTATCC(6/5)
BcnI 3812 CCGGG CC^[GC]GG
BglII 263 AGATCT A^GATCT
BsaBI 3833 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1959 GGCGCC G[GA]^CG[CT]C
BseAI 960 TCCGGA T^CCGGA
BsmBI 2098 CGTCTC CGTCTC(1/5)
Bsp1407I 3968 TGTACA T^GTACA
BspEI 960 TCCGGA T^CCGGA
BspHI 18 TCATGA T^CATGA
BsrGI 3968 TGTACA T^GTACA
Bst1107I 1181 GTATAC GTA^TAC
BstZ17I 1181 GTATAC GTA^TAC
Bsu36I 2816 CCTGAGG CC^T[ACGT]AGG
Eco81I 2816 CCTGAGG CC^T[ACGT]AGG
EcoRI 3943 GAATTC G^AATTC
EcoT22I 1807 ATGCAT ATGCA^T
FspI 1106 TGCGCA TGC^GCA
HaeII 1959 GGCGCC [GA]GCGC^[CT]
Hin1I 1959 GGCGCC G[GA]^CG[CT]C
KasI 1959 GGCGCC G^GCGCC
MamI 3833 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 107 ACGCGT A^CGCGT
MroI 960 TCCGGA T^CCGGA
NarI 1959 GGCGCC GG^CGCC
NciI 3812 CCGGG CC^[GC]GG
NcoI 3225 CCATGG C^CATGG
NsiI 1807 ATGCAT ATGCA^T
PflFI 3711 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 25 CACGTG CAC^GTG
PmlI 25 CACGTG CAC^GTG
RcaI 18 TCATGA T^CATGA
SacI 1609 GAGCTC GAGCT^C
SbfI 3021 CCTGCAGG CCTGCA^GG
SfoI 1959 GGCGCC GGC^GCC
SphI 1171 GCATGC GCATG^C
Sse8387I 3021 CCTGCAGG CCTGCA^GG
SspBI 3968 TGTACA T^GTACA
SspI 655 AATATT AAT^ATT
StuI 2016 AGGCCT AGG^CCT
TaqI 2445 TCGA T^CGA
Tth111I 3711 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2445 TCGA T^CGA
XmnI 2105 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 108 CGCG CG^CG
3408 CGCG
AflII 664 CTTAAG C^TTAAG
3667 CTTAAG
AhdI 3178 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3236 GACATGGTGTC
AlwI 472 GATCC GGATC(4/5)
195 GGATC
AspEI 3178 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3236 GACATGGTGTC
BclI 1818 TGATCA T^GATCA
2614 TGATCA
BfrI 664 CTTAAG C^TTAAG
3667 CTTAAG
BglI 1515 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2958 GCCCCTTTGGC
BlpI 2295 GCTTAGC GC^T[ACGT]AGC
3781 GCTGAGC
Bpu1102I 2295 GCTTAGC GC^T[ACGT]AGC
3781 GCTGAGC
BsaAI 25 CACGTG [CT]AC^GT[GA]
3640 TACGTG
BsaWI 960 TCCGGA [AT]^CCGG[AT]
3707 ACCGGA
BsrDI 1725 CATTGC GCAATG(2/0)
2728 CATTGC
BstUI 108 CGCG CG^CG
3408 CGCG
BstYI 263 AGATCT [GA]^GATC[CT]
471 AGATCC
CelII 2295 GCTTAGC GC^T[ACGT]AGC
3781 GCTGAGC
CfoI 1107 GCGC GCG^C
1960 GCGC
DraI 836 TTTAAA TTT^AAA
1031 TTTAAA
DsaI 2795 CCGTGG C^C[GA][CT]GG
3225 CCATGG
Eam1105I 3178 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3236 GACATGGTGTC
FauI 1769 CCCGC CCCGC(4/6)
2375 CCCGC
FbaI 1818 TGATCA T^GATCA
2614 TGATCA
HhaI 1107 GCGC GCG^C
1960 GCGC
HinP1I 1107 GCGC G^CGC
1960 GCGC
MflI 263 AGATCT [GA]^GATC[CT]
471 AGATCC
MvnI 108 CGCG CG^CG
3408 CGCG
SmlI 664 CTTAAG C^T[CT][GA]AG
3667 CTTAAG
XcmI 485 CCACCTCCTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2290 CCACAGCTTAGCTGG
XhoII 263 AGATCT [GA]^GATC[CT]
471 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwNI ApoI AspHI AvaII
AvrII BalI BanI BanII BbsI
BbvI BfaI BlnI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspLU11I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI DpnII DraII
DraIII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I