################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII Acc65I AclI AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AvaI BamHI BsiEI BsiWI BsoBI BspDI BsrBI BsrFI BssHII BssSI BstBI BstEII BstPI Cfr10I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FseI HgaI HincII HindII HpaI KpnI KspI MfeI MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccIII 960 TCCGGA T^CCGGA AcyI 1959 GGCGCC G[GA]^CG[CT]C AocI 2816 CCTGAGG CC^T[ACGT]AGG Asp700I 2105 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 3711 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1106 TGCGCA TGC^GCA BbeI 1959 GGCGCC GGCGC^C BbrPI 25 CACGTG CAC^GTG BbvCI 712 GCTGAGG CCTCAGC(-5/-2) BcgI 1626 CGAGAACCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1463 GTATCC GTATCC(6/5) BcnI 3812 CCGGG CC^[GC]GG BglII 263 AGATCT A^GATCT BsaBI 3833 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1959 GGCGCC G[GA]^CG[CT]C BseAI 960 TCCGGA T^CCGGA BsmBI 2098 CGTCTC CGTCTC(1/5) Bsp1407I 3968 TGTACA T^GTACA BspEI 960 TCCGGA T^CCGGA BspHI 18 TCATGA T^CATGA BsrGI 3968 TGTACA T^GTACA Bst1107I 1181 GTATAC GTA^TAC BstZ17I 1181 GTATAC GTA^TAC Bsu36I 2816 CCTGAGG CC^T[ACGT]AGG Eco81I 2816 CCTGAGG CC^T[ACGT]AGG EcoRI 3943 GAATTC G^AATTC EcoT22I 1807 ATGCAT ATGCA^T FspI 1106 TGCGCA TGC^GCA HaeII 1959 GGCGCC [GA]GCGC^[CT] Hin1I 1959 GGCGCC G[GA]^CG[CT]C KasI 1959 GGCGCC G^GCGCC MamI 3833 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 107 ACGCGT A^CGCGT MroI 960 TCCGGA T^CCGGA NarI 1959 GGCGCC GG^CGCC NciI 3812 CCGGG CC^[GC]GG NcoI 3225 CCATGG C^CATGG NsiI 1807 ATGCAT ATGCA^T PflFI 3711 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 25 CACGTG CAC^GTG PmlI 25 CACGTG CAC^GTG RcaI 18 TCATGA T^CATGA SacI 1609 GAGCTC GAGCT^C SbfI 3021 CCTGCAGG CCTGCA^GG SfoI 1959 GGCGCC GGC^GCC SphI 1171 GCATGC GCATG^C Sse8387I 3021 CCTGCAGG CCTGCA^GG SspBI 3968 TGTACA T^GTACA SspI 655 AATATT AAT^ATT StuI 2016 AGGCCT AGG^CCT TaqI 2445 TCGA T^CGA Tth111I 3711 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 2445 TCGA T^CGA XmnI 2105 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 108 CGCG CG^CG 3408 CGCG AflII 664 CTTAAG C^TTAAG 3667 CTTAAG AhdI 3178 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3236 GACATGGTGTC AlwI 472 GATCC GGATC(4/5) 195 GGATC AspEI 3178 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3236 GACATGGTGTC BclI 1818 TGATCA T^GATCA 2614 TGATCA BfrI 664 CTTAAG C^TTAAG 3667 CTTAAG BglI 1515 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2958 GCCCCTTTGGC BlpI 2295 GCTTAGC GC^T[ACGT]AGC 3781 GCTGAGC Bpu1102I 2295 GCTTAGC GC^T[ACGT]AGC 3781 GCTGAGC BsaAI 25 CACGTG [CT]AC^GT[GA] 3640 TACGTG BsaWI 960 TCCGGA [AT]^CCGG[AT] 3707 ACCGGA BsrDI 1725 CATTGC GCAATG(2/0) 2728 CATTGC BstUI 108 CGCG CG^CG 3408 CGCG BstYI 263 AGATCT [GA]^GATC[CT] 471 AGATCC CelII 2295 GCTTAGC GC^T[ACGT]AGC 3781 GCTGAGC CfoI 1107 GCGC GCG^C 1960 GCGC DraI 836 TTTAAA TTT^AAA 1031 TTTAAA DsaI 2795 CCGTGG C^C[GA][CT]GG 3225 CCATGG Eam1105I 3178 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3236 GACATGGTGTC FauI 1769 CCCGC CCCGC(4/6) 2375 CCCGC FbaI 1818 TGATCA T^GATCA 2614 TGATCA HhaI 1107 GCGC GCG^C 1960 GCGC HinP1I 1107 GCGC G^CGC 1960 GCGC MflI 263 AGATCT [GA]^GATC[CT] 471 AGATCC MvnI 108 CGCG CG^CG 3408 CGCG SmlI 664 CTTAAG C^T[CT][GA]AG 3667 CTTAAG XcmI 485 CCACCTCCTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2290 CCACAGCTTAGCTGG XhoII 263 AGATCT [GA]^GATC[CT] 471 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwNI ApoI AspHI AvaII AvrII BalI BanI BanII BbsI BbvI BfaI BlnI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspLU11I BspMI BsrI BssKI BstAPI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DraII DraIII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I