################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AccIII AclI AflIII AgeI AscI AseI AsnI BciVI BclI BglII BsaBI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BstBI ClaI CpoI DrdI EagI EclXI Eco52I EcoRV FbaI FseI HgaI HindIII HpaI KspI MamI MfeI MluI MroI MunI NotI NruI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SspBI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 342 GACGTC GACGT^C Acc65I 4082 GGTACC G^GTACC AflII 4038 CTTAAG C^TTAAG AhdI 4050 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 14 AGCGCT AGC^GCT Asp700I 2854 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 4082 GGTACC G^GTACC AspEI 4050 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2038 TGCGCA TGC^GCA AvrII 4045 CCTAGG C^CTAGG BamHI 4254 GGATCC G^GATCC BbrPI 1593 CACGTG CAC^GTG BfrI 4038 CTTAAG C^TTAAG BlnI 4045 CCTAGG C^CTAGG BlpI 3292 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3292 GCTGAGC GC^T[ACGT]AGC BsaAI 1593 CACGTG [CT]AC^GT[GA] BsaWI 226 TCCGGT [AT]^CCGG[AT] BsmI 1199 GCATTC GAATGC(1/-1) BsrBI 43 GAGCGG CCGCTC(-3/-3) BsrDI 2601 CATTGC GCAATG(2/0) CelII 3292 GCTGAGC GC^T[ACGT]AGC Eam1105I 4050 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 14 AGCGCT AGC^GCT EcoRI 3267 GAATTC G^AATTC EcoT22I 2255 ATGCAT ATGCA^T FspI 2038 TGCGCA TGC^GCA KpnI 4082 GGTACC GGTAC^C NdeI 1522 CATATG CA^TATG NsiI 2255 ATGCAT ATGCA^T PaeR7I 169 CTCGAG C^TCGAG PmaCI 1593 CACGTG CAC^GTG PmlI 1593 CACGTG CAC^GTG PshAI 1716 GACTCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 2383 CCTGCAGG CCTGCA^GG SpeI 4212 ACTAGT A^CTAGT Sse8387I 2383 CCTGCAGG CCTGCA^GG SspI 2721 AATATT AAT^ATT XhoI 169 CTCGAG C^TCGAG XmnI 2854 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccI 1491 GTATAC GT^[AC][GT]AC 2211 GTATAC AspI 459 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1514 GACAGTGTC AvaI 169 CTCGAG C^[CT]CG[GA]G 999 CCCGAG BalI 2416 TGGCCA TGG^CCA 3871 TGGCCA BbeI 94 GGCGCC GGCGC^C 1479 GGCGCC BbsI 1748 GAAGAC GAAGAC(2/6) 1787 GAAGAC BbvCI 1137 GCTGAGG CCTCAGC(-5/-2) 1677 GCTGAGG BcgI 1044 GCACCCACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2450 GCAGGGACCTCG BcnI 374 CCGGG CC^[GC]GG 2776 CCGGG BpuAI 1748 GAAGAC GAAGAC(2/6) 1787 GAAGAC BsaI 1647 GAGACC GGTCTC(1/5) 3812 GAGACC BsoBI 169 CTCGAG C^[CT]CG[GA]G 999 CCCGAG BssSI 1782 CACGAG CACGAG(-5/-1) 3089 CTCGTG Bst1107I 1491 GTATAC GTA^TAC 2211 GTATAC BstAPI 2257 GCATGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3993 GCAGGGCATGC BstXI 1120 CCATGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1988 CCAGCTCTGTGG BstZ17I 1491 GTATAC GTA^TAC 2211 GTATAC DraI 2689 TTTAAA TTT^AAA 2703 TTTAAA DraIII 1693 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG 3883 CACATAGTG KasI 94 GGCGCC G^GCGCC 1479 GGCGCC MaeII 343 ACGT A^CGT 1594 ACGT MluNI 2416 TGGCCA TGG^CCA 3871 TGGCCA MscI 2416 TGGCCA TGG^CCA 3871 TGGCCA NaeI 51 GCCGGC GCC^GGC 188 GCCGGC NarI 94 GGCGCC GG^CGCC 1479 GGCGCC NciI 374 CCGGG CC^[GC]GG 2776 CCGGG NcoI 1120 CCATGG C^CATGG 3616 CCATGG NgoMIV 51 GCCGGC G^CCGGC 188 GCCGGC PflFI 459 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1514 GACAGTGTC PflMI 858 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2344 CCATCATGTGG SacI 771 GAGCTC GAGCT^C 3524 GAGCTC SfoI 94 GGCGCC GGC^GCC 1479 GGCGCC SmlI 169 CTCGAG C^T[CT][GA]AG 4038 CTTAAG StuI 2439 AGGCCT AGG^CCT 4042 AGGCCT Tth111I 459 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1514 GACAGTGTC Van91I 858 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2344 CCATCATGTGG XcmI 1327 CCAATGGCGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3348 CCAAGCCCCTTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AspHI AvaII BanI BanII BbvI BfaI BglI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssHII BssKI BstEII BstF5I BstNI BstPI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NheI NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII