##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AccIII AclI AflIII AgeI AscI
AseI AsnI BciVI BclI BglII
BsaBI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BstBI ClaI CpoI DrdI
EagI EclXI Eco52I EcoRV FbaI
FseI HgaI HindIII HpaI KspI
MamI MfeI MluI MroI MunI
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SspBI SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 342 GACGTC GACGT^C
Acc65I 4082 GGTACC G^GTACC
AflII 4038 CTTAAG C^TTAAG
AhdI 4050 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 14 AGCGCT AGC^GCT
Asp700I 2854 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4082 GGTACC G^GTACC
AspEI 4050 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2038 TGCGCA TGC^GCA
AvrII 4045 CCTAGG C^CTAGG
BamHI 4254 GGATCC G^GATCC
BbrPI 1593 CACGTG CAC^GTG
BfrI 4038 CTTAAG C^TTAAG
BlnI 4045 CCTAGG C^CTAGG
BlpI 3292 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3292 GCTGAGC GC^T[ACGT]AGC
BsaAI 1593 CACGTG [CT]AC^GT[GA]
BsaWI 226 TCCGGT [AT]^CCGG[AT]
BsmI 1199 GCATTC GAATGC(1/-1)
BsrBI 43 GAGCGG CCGCTC(-3/-3)
BsrDI 2601 CATTGC GCAATG(2/0)
CelII 3292 GCTGAGC GC^T[ACGT]AGC
Eam1105I 4050 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 14 AGCGCT AGC^GCT
EcoRI 3267 GAATTC G^AATTC
EcoT22I 2255 ATGCAT ATGCA^T
FspI 2038 TGCGCA TGC^GCA
KpnI 4082 GGTACC GGTAC^C
NdeI 1522 CATATG CA^TATG
NsiI 2255 ATGCAT ATGCA^T
PaeR7I 169 CTCGAG C^TCGAG
PmaCI 1593 CACGTG CAC^GTG
PmlI 1593 CACGTG CAC^GTG
PshAI 1716 GACTCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 2383 CCTGCAGG CCTGCA^GG
SpeI 4212 ACTAGT A^CTAGT
Sse8387I 2383 CCTGCAGG CCTGCA^GG
SspI 2721 AATATT AAT^ATT
XhoI 169 CTCGAG C^TCGAG
XmnI 2854 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1491 GTATAC GT^[AC][GT]AC
2211 GTATAC
AspI 459 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1514 GACAGTGTC
AvaI 169 CTCGAG C^[CT]CG[GA]G
999 CCCGAG
BalI 2416 TGGCCA TGG^CCA
3871 TGGCCA
BbeI 94 GGCGCC GGCGC^C
1479 GGCGCC
BbsI 1748 GAAGAC GAAGAC(2/6)
1787 GAAGAC
BbvCI 1137 GCTGAGG CCTCAGC(-5/-2)
1677 GCTGAGG
BcgI 1044 GCACCCACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2450 GCAGGGACCTCG
BcnI 374 CCGGG CC^[GC]GG
2776 CCGGG
BpuAI 1748 GAAGAC GAAGAC(2/6)
1787 GAAGAC
BsaI 1647 GAGACC GGTCTC(1/5)
3812 GAGACC
BsoBI 169 CTCGAG C^[CT]CG[GA]G
999 CCCGAG
BssSI 1782 CACGAG CACGAG(-5/-1)
3089 CTCGTG
Bst1107I 1491 GTATAC GTA^TAC
2211 GTATAC
BstAPI 2257 GCATGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3993 GCAGGGCATGC
BstXI 1120 CCATGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1988 CCAGCTCTGTGG
BstZ17I 1491 GTATAC GTA^TAC
2211 GTATAC
DraI 2689 TTTAAA TTT^AAA
2703 TTTAAA
DraIII 1693 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
3883 CACATAGTG
KasI 94 GGCGCC G^GCGCC
1479 GGCGCC
MaeII 343 ACGT A^CGT
1594 ACGT
MluNI 2416 TGGCCA TGG^CCA
3871 TGGCCA
MscI 2416 TGGCCA TGG^CCA
3871 TGGCCA
NaeI 51 GCCGGC GCC^GGC
188 GCCGGC
NarI 94 GGCGCC GG^CGCC
1479 GGCGCC
NciI 374 CCGGG CC^[GC]GG
2776 CCGGG
NcoI 1120 CCATGG C^CATGG
3616 CCATGG
NgoMIV 51 GCCGGC G^CCGGC
188 GCCGGC
PflFI 459 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1514 GACAGTGTC
PflMI 858 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2344 CCATCATGTGG
SacI 771 GAGCTC GAGCT^C
3524 GAGCTC
SfoI 94 GGCGCC GGC^GCC
1479 GGCGCC
SmlI 169 CTCGAG C^T[CT][GA]AG
4038 CTTAAG
StuI 2439 AGGCCT AGG^CCT
4042 AGGCCT
Tth111I 459 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1514 GACAGTGTC
Van91I 858 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2344 CCATCATGTGG
XcmI 1327 CCAATGGCGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3348 CCAAGCCCCTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AspHI AvaII
BanI BanII BbvI BfaI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssHII BssKI BstEII BstF5I BstNI
BstPI BstUI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NheI NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII