##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AgeI        
AhdI        Alw44I      AocI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvrII       BbeI        BbrPI       BcgI        BclI        
BglII       BlnI        BsaAI       BsaBI       BsaHI       
BsiWI       BssHII      BssSI       BstBI       Bsu36I      
DrdI        Eam1105I    Eco81I      EcoRV       FbaI        
Hin1I       HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MunI        NarI        
NotI        NruI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        SacII       SalI        SbfI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
Sse8387I    SwaI        Tth111I     


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccIII        181  TCCGGA               T^CCGGA
AlwNI        1034  CAGGATCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI        62  AGCGCT               AGC^GCT
Asp700I      1709  GAAACACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         767  GAGCTC               G[AT]GC[AT]^C
AviII        2378  TGCGCA               TGC^GCA
BalI         2324  TGGCCA               TGG^CCA
BbvCI         477  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1201  GTATCC               GTATCC(6/5)
BglI          114  GCCCGGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         2071  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2071  GCTGAGC              GC^T[ACGT]AGC
BsaI         2101  GAGACC               GGTCTC(1/5)
BseAI         181  TCCGGA               T^CCGGA
BsiHKAI       767  GAGCTC               G[AT]GC[AT]^C
BsmBI         710  GAGACG               CGTCTC(1/5)
BspDI        2922  ATCGAT               AT^CGAT
BspEI         181  TCCGGA               T^CCGGA
BspHI        1332  TCATGA               T^CATGA
Bst1107I      916  GTATAC               GTA^TAC
BstAPI       1852  GCAGATGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2265  GGTGACC              G^GT[ACGT]ACC
BstPI        2265  GGTGACC              G^GT[ACGT]ACC
BstZ17I       916  GTATAC               GTA^TAC
CelII        2071  GCTGAGC              GC^T[ACGT]AGC
ClaI         2922  ATCGAT               AT^CGAT
CpoI         2959  CGGTCCG              CG^G[AT]CCG
Eco47III       62  AGCGCT               AGC^GCT
EcoO65I      2265  GGTGACC              G^GT[ACGT]ACC
FseI          118  GGCCGGCC             GGCCGG^CC
FspI         2378  TGCGCA               TGC^GCA
HgaI         2133  GCGTC                GACGC(5/10)
HincII       4439  GTCAAC               GT[CT]^[GA]AC
HindII       4439  GTCAAC               GT[CT]^[GA]AC
HindIII      3416  AAGCTT               A^AGCTT
MluNI        2324  TGGCCA               TGG^CCA
MroI          181  TCCGGA               T^CCGGA
MscI         2324  TGGCCA               TGG^CCA
PaeR7I       1188  CTCGAG               C^TCGAG
PflMI        1371  CCACCCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        2570  AGGTCCT              [GA]G^G[AT]CC[CT]
RcaI         1332  TCATGA               T^CATGA
RsrII        2959  CGGTCCG              CG^G[AT]CCG
SacI          767  GAGCTC               GAGCT^C
ScaI         3392  AGTACT               AGT^ACT
SmaI           46  CCCGGG               CCC^GGG
SpeI            4  ACTAGT               A^CTAGT
SspI         3614  AATATT               AAT^ATT
StuI         3368  AGGCCT               AGG^CCT
Van91I       1371  CCACCCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         3468  TCTAGA               T^CTAGA
XhoI         1188  CTCGAG               C^TCGAG
XmaCI          46  CCCGGG               C^CCGGG
XmaI           46  CCCGGG               C^CCGGG
XmnI         1709  GAAACACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccII         634  CGCG                 CG^CG
              636  CGCG                
AflII        1575  CTTAAG               C^TTAAG
             4224  CTTAAG              
AvaI           46  CCCGGG               C^[CT]CG[GA]G
             1188  CTCGAG              
BbsI         1264  GAAGAC               GAAGAC(2/6)
             3533  GAAGAC              
BfrI         1575  CTTAAG               C^TTAAG
             4224  CTTAAG              
BpuAI        1264  GAAGAC               GAAGAC(2/6)
             3533  GAAGAC              
BsiEI         117  CGGCCG               CG[GA][CT]^CG
              121  CGGCCG              
BsmI          374  GAATGC               GAATGC(1/-1)
             4483  GCATTC              
BsoBI          46  CCCGGG               C^[CT]CG[GA]G
             1188  CTCGAG              
BspLU11I     3537  ACATGT               A^CATGT
             3575  ACATGT              
BsrBI        2963  CCGCTC               CCGCTC(-3/-3)
             2185  GAGCGG              
BsrFI         119  GCCGGC               [GA]^CCGG[CT]
              123  GCCGGC              
BstUI         634  CGCG                 CG^CG
              636  CGCG                
BstXI        1848  CCAAGCAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2598  CCATGCCCCTGG        
Cfr10I        119  GCCGGC               [GA]^CCGG[CT]
              123  GCCGGC              
EagI          117  CGGCCG               C^GGCCG
              121  CGGCCG              
EclXI         117  CGGCCG               C^GGCCG
              121  CGGCCG              
Eco52I        117  CGGCCG               C^GGCCG
              121  CGGCCG              
EcoNI        2703  CCTGGATAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3025  CCTCACGCAGG         
EcoRI        1121  GAATTC               G^AATTC
             4546  GAATTC              
FauI         1596  CCCGC                CCCGC(4/6)
             2741  CCCGC               
HaeII          62  AGCGCT               [GA]GCGC^[CT]
             1228  AGCGCC              
MvnI          634  CGCG                 CG^CG
              636  CGCG                
NaeI          119  GCCGGC               GCC^GGC
              123  GCCGGC              
NcoI         1362  CCATGG               C^CATGG
             2546  CCATGG              
NdeI         1468  CATATG               CA^TATG
             4345  CATATG              
NgoMIV        119  GCCGGC               G^CCGGC
              123  GCCGGC              
NheI         3831  GCTAGC               G^CTAGC
             3920  GCTAGC              
PshAI        1490  GACAAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2460  GACAGATGTC          
SapI         2182  GAAGAGC              GCTCTTC(1/4)
             2413  GAAGAGC             
SexAI        1283  ACCTGGT              A^CC[AT]GGT
             4057  ACCTGGT             


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        ApaI        ApoI        AvaII       
BamHI       BanI        BanII       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaJI       BsaWI       
BseRI       BsgI        BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    Bsp1407I    BspMI       BsrDI       
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       DraIII      DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
EcoT22I     Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SphI        SspBI       StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII