##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BbeI BbrPI BcgI BclI
BglII BlnI BsaAI BsaBI BsaHI
BsiWI BssHII BssSI BstBI Bsu36I
DrdI Eam1105I Eco81I EcoRV FbaI
Hin1I HpaI KasI KpnI KspI
MamI MfeI MluI MunI NarI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI SacII SalI SbfI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccIII 181 TCCGGA T^CCGGA
AlwNI 1034 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 62 AGCGCT AGC^GCT
Asp700I 1709 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 767 GAGCTC G[AT]GC[AT]^C
AviII 2378 TGCGCA TGC^GCA
BalI 2324 TGGCCA TGG^CCA
BbvCI 477 GCTGAGG CCTCAGC(-5/-2)
BciVI 1201 GTATCC GTATCC(6/5)
BglI 114 GCCCGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2071 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2071 GCTGAGC GC^T[ACGT]AGC
BsaI 2101 GAGACC GGTCTC(1/5)
BseAI 181 TCCGGA T^CCGGA
BsiHKAI 767 GAGCTC G[AT]GC[AT]^C
BsmBI 710 GAGACG CGTCTC(1/5)
BspDI 2922 ATCGAT AT^CGAT
BspEI 181 TCCGGA T^CCGGA
BspHI 1332 TCATGA T^CATGA
Bst1107I 916 GTATAC GTA^TAC
BstAPI 1852 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2265 GGTGACC G^GT[ACGT]ACC
BstPI 2265 GGTGACC G^GT[ACGT]ACC
BstZ17I 916 GTATAC GTA^TAC
CelII 2071 GCTGAGC GC^T[ACGT]AGC
ClaI 2922 ATCGAT AT^CGAT
CpoI 2959 CGGTCCG CG^G[AT]CCG
Eco47III 62 AGCGCT AGC^GCT
EcoO65I 2265 GGTGACC G^GT[ACGT]ACC
FseI 118 GGCCGGCC GGCCGG^CC
FspI 2378 TGCGCA TGC^GCA
HgaI 2133 GCGTC GACGC(5/10)
HincII 4439 GTCAAC GT[CT]^[GA]AC
HindII 4439 GTCAAC GT[CT]^[GA]AC
HindIII 3416 AAGCTT A^AGCTT
MluNI 2324 TGGCCA TGG^CCA
MroI 181 TCCGGA T^CCGGA
MscI 2324 TGGCCA TGG^CCA
PaeR7I 1188 CTCGAG C^TCGAG
PflMI 1371 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2570 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1332 TCATGA T^CATGA
RsrII 2959 CGGTCCG CG^G[AT]CCG
SacI 767 GAGCTC GAGCT^C
ScaI 3392 AGTACT AGT^ACT
SmaI 46 CCCGGG CCC^GGG
SpeI 4 ACTAGT A^CTAGT
SspI 3614 AATATT AAT^ATT
StuI 3368 AGGCCT AGG^CCT
Van91I 1371 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3468 TCTAGA T^CTAGA
XhoI 1188 CTCGAG C^TCGAG
XmaCI 46 CCCGGG C^CCGGG
XmaI 46 CCCGGG C^CCGGG
XmnI 1709 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 634 CGCG CG^CG
636 CGCG
AflII 1575 CTTAAG C^TTAAG
4224 CTTAAG
AvaI 46 CCCGGG C^[CT]CG[GA]G
1188 CTCGAG
BbsI 1264 GAAGAC GAAGAC(2/6)
3533 GAAGAC
BfrI 1575 CTTAAG C^TTAAG
4224 CTTAAG
BpuAI 1264 GAAGAC GAAGAC(2/6)
3533 GAAGAC
BsiEI 117 CGGCCG CG[GA][CT]^CG
121 CGGCCG
BsmI 374 GAATGC GAATGC(1/-1)
4483 GCATTC
BsoBI 46 CCCGGG C^[CT]CG[GA]G
1188 CTCGAG
BspLU11I 3537 ACATGT A^CATGT
3575 ACATGT
BsrBI 2963 CCGCTC CCGCTC(-3/-3)
2185 GAGCGG
BsrFI 119 GCCGGC [GA]^CCGG[CT]
123 GCCGGC
BstUI 634 CGCG CG^CG
636 CGCG
BstXI 1848 CCAAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2598 CCATGCCCCTGG
Cfr10I 119 GCCGGC [GA]^CCGG[CT]
123 GCCGGC
EagI 117 CGGCCG C^GGCCG
121 CGGCCG
EclXI 117 CGGCCG C^GGCCG
121 CGGCCG
Eco52I 117 CGGCCG C^GGCCG
121 CGGCCG
EcoNI 2703 CCTGGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3025 CCTCACGCAGG
EcoRI 1121 GAATTC G^AATTC
4546 GAATTC
FauI 1596 CCCGC CCCGC(4/6)
2741 CCCGC
HaeII 62 AGCGCT [GA]GCGC^[CT]
1228 AGCGCC
MvnI 634 CGCG CG^CG
636 CGCG
NaeI 119 GCCGGC GCC^GGC
123 GCCGGC
NcoI 1362 CCATGG C^CATGG
2546 CCATGG
NdeI 1468 CATATG CA^TATG
4345 CATATG
NgoMIV 119 GCCGGC G^CCGGC
123 GCCGGC
NheI 3831 GCTAGC G^CTAGC
3920 GCTAGC
PshAI 1490 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2460 GACAGATGTC
SapI 2182 GAAGAGC GCTCTTC(1/4)
2413 GAAGAGC
SexAI 1283 ACCTGGT A^CC[AT]GGT
4057 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApaI ApoI AvaII
BamHI BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BsaWI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII