################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII Acc65I AclI AcyI AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvrII BbeI BbrPI BcgI BclI BglII BlnI BsaAI BsaBI BsaHI BsiWI BssHII BssSI BstBI Bsu36I DrdI Eam1105I Eco81I EcoRV FbaI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MunI NarI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SwaI Tth111I ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccIII 181 TCCGGA T^CCGGA AlwNI 1034 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 62 AGCGCT AGC^GCT Asp700I 1709 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 767 GAGCTC G[AT]GC[AT]^C AviII 2378 TGCGCA TGC^GCA BalI 2324 TGGCCA TGG^CCA BbvCI 477 GCTGAGG CCTCAGC(-5/-2) BciVI 1201 GTATCC GTATCC(6/5) BglI 114 GCCCGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 2071 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2071 GCTGAGC GC^T[ACGT]AGC BsaI 2101 GAGACC GGTCTC(1/5) BseAI 181 TCCGGA T^CCGGA BsiHKAI 767 GAGCTC G[AT]GC[AT]^C BsmBI 710 GAGACG CGTCTC(1/5) BspDI 2922 ATCGAT AT^CGAT BspEI 181 TCCGGA T^CCGGA BspHI 1332 TCATGA T^CATGA Bst1107I 916 GTATAC GTA^TAC BstAPI 1852 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2265 GGTGACC G^GT[ACGT]ACC BstPI 2265 GGTGACC G^GT[ACGT]ACC BstZ17I 916 GTATAC GTA^TAC CelII 2071 GCTGAGC GC^T[ACGT]AGC ClaI 2922 ATCGAT AT^CGAT CpoI 2959 CGGTCCG CG^G[AT]CCG Eco47III 62 AGCGCT AGC^GCT EcoO65I 2265 GGTGACC G^GT[ACGT]ACC FseI 118 GGCCGGCC GGCCGG^CC FspI 2378 TGCGCA TGC^GCA HgaI 2133 GCGTC GACGC(5/10) HincII 4439 GTCAAC GT[CT]^[GA]AC HindII 4439 GTCAAC GT[CT]^[GA]AC HindIII 3416 AAGCTT A^AGCTT MluNI 2324 TGGCCA TGG^CCA MroI 181 TCCGGA T^CCGGA MscI 2324 TGGCCA TGG^CCA PaeR7I 1188 CTCGAG C^TCGAG PflMI 1371 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 2570 AGGTCCT [GA]G^G[AT]CC[CT] RcaI 1332 TCATGA T^CATGA RsrII 2959 CGGTCCG CG^G[AT]CCG SacI 767 GAGCTC GAGCT^C ScaI 3392 AGTACT AGT^ACT SmaI 46 CCCGGG CCC^GGG SpeI 4 ACTAGT A^CTAGT SspI 3614 AATATT AAT^ATT StuI 3368 AGGCCT AGG^CCT Van91I 1371 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3468 TCTAGA T^CTAGA XhoI 1188 CTCGAG C^TCGAG XmaCI 46 CCCGGG C^CCGGG XmaI 46 CCCGGG C^CCGGG XmnI 1709 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 634 CGCG CG^CG 636 CGCG AflII 1575 CTTAAG C^TTAAG 4224 CTTAAG AvaI 46 CCCGGG C^[CT]CG[GA]G 1188 CTCGAG BbsI 1264 GAAGAC GAAGAC(2/6) 3533 GAAGAC BfrI 1575 CTTAAG C^TTAAG 4224 CTTAAG BpuAI 1264 GAAGAC GAAGAC(2/6) 3533 GAAGAC BsiEI 117 CGGCCG CG[GA][CT]^CG 121 CGGCCG BsmI 374 GAATGC GAATGC(1/-1) 4483 GCATTC BsoBI 46 CCCGGG C^[CT]CG[GA]G 1188 CTCGAG BspLU11I 3537 ACATGT A^CATGT 3575 ACATGT BsrBI 2963 CCGCTC CCGCTC(-3/-3) 2185 GAGCGG BsrFI 119 GCCGGC [GA]^CCGG[CT] 123 GCCGGC BstUI 634 CGCG CG^CG 636 CGCG BstXI 1848 CCAAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2598 CCATGCCCCTGG Cfr10I 119 GCCGGC [GA]^CCGG[CT] 123 GCCGGC EagI 117 CGGCCG C^GGCCG 121 CGGCCG EclXI 117 CGGCCG C^GGCCG 121 CGGCCG Eco52I 117 CGGCCG C^GGCCG 121 CGGCCG EcoNI 2703 CCTGGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3025 CCTCACGCAGG EcoRI 1121 GAATTC G^AATTC 4546 GAATTC FauI 1596 CCCGC CCCGC(4/6) 2741 CCCGC HaeII 62 AGCGCT [GA]GCGC^[CT] 1228 AGCGCC MvnI 634 CGCG CG^CG 636 CGCG NaeI 119 GCCGGC GCC^GGC 123 GCCGGC NcoI 1362 CCATGG C^CATGG 2546 CCATGG NdeI 1468 CATATG CA^TATG 4345 CATATG NgoMIV 119 GCCGGC G^CCGGC 123 GCCGGC NheI 3831 GCTAGC G^CTAGC 3920 GCTAGC PshAI 1490 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2460 GACAGATGTC SapI 2182 GAAGAGC GCTCTTC(1/4) 2413 GAAGAGC SexAI 1283 ACCTGGT A^CC[AT]GGT 4057 ACCTGGT ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI ApaI ApoI AvaII BamHI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII