##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AccIII AclI AgeI AscI
AseI AsnI Asp718I AviII BcgI
BglI BlpI Bpu1102I BseAI BsiEI
BsiWI BspDI BspEI BssHII BstAPI
BstBI CelII ClaI CpoI EagI
EclXI Eco52I EcoT22I FseI FspI
KpnI KspI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NsiI NspV PacI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
Van91I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 3183 GACGTC GACGT^C
AccI 4395 GTATAC GT^[AC][GT]AC
AccII 695 CGCG CG^CG
AflII 3509 CTTAAG C^TTAAG
Alw44I 240 GTGCAC G^TGCAC
Aor51HI 3232 AGCGCT AGC^GCT
ApaI 1247 GGGCCC GGGCC^C
ApaLI 240 GTGCAC G^TGCAC
AspI 330 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4267 CCTAGG C^CTAGG
BamHI 1093 GGATCC G^GATCC
BanI 222 GGCGCC G^G[CT][GA]CC
BbeI 222 GGCGCC GGCGC^C
BbrPI 4590 CACGTG CAC^GTG
BclI 2033 TGATCA T^GATCA
BfrI 3509 CTTAAG C^TTAAG
BlnI 4267 CCTAGG C^CTAGG
BsaAI 4590 CACGTG [CT]AC^GT[GA]
BsaI 208 GGTCTC GGTCTC(1/5)
BsaWI 3051 ACCGGA [AT]^CCGG[AT]
BsmI 1198 GAATGC GAATGC(1/-1)
Bsp1407I 2224 TGTACA T^GTACA
BsrBI 2946 GAGCGG CCGCTC(-3/-3)
BsrDI 757 GCAATG GCAATG(2/0)
BsrGI 2224 TGTACA T^GTACA
Bst1107I 4395 GTATAC GTA^TAC
BstUI 695 CGCG CG^CG
BstZ17I 4395 GTATAC GTA^TAC
DraI 4553 TTTAAA TTT^AAA
DrdI 2563 GACTTGAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2253 CCACGG C^C[GA][CT]GG
Eco47III 3232 AGCGCT AGC^GCT
FbaI 2033 TGATCA T^GATCA
HindIII 1307 AAGCTT A^AGCTT
KasI 222 GGCGCC G^GCGCC
MvnI 695 CGCG CG^CG
NarI 222 GGCGCC GG^CGCC
NruI 694 TCGCGA TCG^CGA
PaeR7I 4584 CTCGAG C^TCGAG
PflFI 330 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4590 CACGTG CAC^GTG
PmlI 4590 CACGTG CAC^GTG
SapI 1962 GAAGAGC GCTCTTC(1/4)
SfoI 222 GGCGCC GGC^GCC
SphI 2978 GCATGC GCATG^C
SspBI 2224 TGTACA T^GTACA
Tth111I 330 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4584 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 2627 ACATGT A^C[GA][CT]GT
4096 ACATGT
AhdI 2145 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4480 GACTGGGTGTC
AspEI 2145 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4480 GACTGGGTGTC
BanII 867 GAGCCC G[GA]GC[CT]^C
1247 GGGCCC
BbvCI 905 GCTGAGG CCTCAGC(-5/-2)
4625 GCTGAGG
BglII 3697 AGATCT A^GATCT
3709 AGATCT
BseRI 1578 GAGGAG GAGGAG(10/8)
3282 GAGGAG
BsmBI 1632 CGTCTC CGTCTC(1/5)
3185 CGTCTC
BspHI 53 TCATGA T^CATGA
1441 TCATGA
BspLU11I 2627 ACATGT A^CATGT
4096 ACATGT
BssSI 3402 CACGAG CACGAG(-5/-1)
885 CTCGTG
BstEII 363 GGTAACC G^GT[ACGT]ACC
3120 GGTGACC
BstPI 363 GGTAACC G^GT[ACGT]ACC
3120 GGTGACC
Eam1105I 2145 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4480 GACTGGGTGTC
EcoNI 1971 CCTAGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4334 CCTGCCTCAGG
EcoO65I 363 GGTAACC G^GT[ACGT]ACC
3120 GGTGACC
EcoRI 1719 GAATTC G^AATTC
4616 GAATTC
EcoRV 2462 GATATC GAT^ATC
4329 GATATC
FauI 3550 CCCGC CCCGC(4/6)
3090 GCGGG
HpaI 3348 GTTAAC GTT^AAC
4169 GTTAAC
NaeI 22 GCCGGC GCC^GGC
3223 GCCGGC
NgoMIV 22 GCCGGC G^CCGGC
3223 GCCGGC
RcaI 53 TCATGA T^CATGA
1441 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaI AvaII BalI BbsI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaBI BsaHI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI