##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI AscI AseI AsnI Asp718I
AviII AvrII BalI BbeI BcgI
BclI BglI BglII BlnI BsaBI
BsaHI BsiEI BsiWI BsmBI BspDI
BssHII BstBI BstUI ClaI CpoI
DraIII DrdI EaeI EagI EclXI
Eco52I EcoRV FbaI FseI FspI
HgaI Hin1I KasI KpnI KspI
MamI MfeI MluI MluNI MscI
MunI MvnI NarI NotI NruI
NspV PaeR7I PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SspI SwaI
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 459 TCCGGA T^CCGGA
AflII 526 CTTAAG C^TTAAG
AhdI 2649 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1044 GTGCAC G^TGCAC
AocI 4042 CCTTAGG CC^T[ACGT]AGG
ApaI 2051 GGGCCC GGGCC^C
ApaLI 1044 GTGCAC G^TGCAC
AspEI 2649 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2949 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3392 CTCGGG C^[CT]CG[GA]G
BbvCI 3847 GCTGAGG CCTCAGC(-5/-2)
BciVI 1769 GGATAC GTATCC(6/5)
BcnI 2017 CCGGG CC^[GC]GG
BfrI 526 CTTAAG C^TTAAG
BsaWI 459 TCCGGA [AT]^CCGG[AT]
BseAI 459 TCCGGA T^CCGGA
BsgI 3647 CTGCAC GTGCAG(16/14)
BsmI 4231 GCATTC GAATGC(1/-1)
BsoBI 3392 CTCGGG C^[CT]CG[GA]G
BspEI 459 TCCGGA T^CCGGA
BspHI 1137 TCATGA T^CATGA
BssSI 2352 CTCGTG CACGAG(-5/-1)
Bst1107I 3486 GTATAC GTA^TAC
BstXI 739 CCAGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3486 GTATAC GTA^TAC
Bsu36I 4042 CCTTAGG CC^T[ACGT]AGG
DsaI 1201 CCATGG C^C[GA][CT]GG
Eam1105I 2649 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4042 CCTTAGG CC^T[ACGT]AGG
EcoT22I 4005 ATGCAT ATGCA^T
HpaI 4274 GTTAAC GTT^AAC
MroI 459 TCCGGA T^CCGGA
NaeI 3932 GCCGGC GCC^GGC
NciI 2017 CCGGG CC^[GC]GG
NcoI 1201 CCATGG C^CATGG
NgoMIV 3932 GCCGGC G^CCGGC
NheI 3944 GCTAGC G^CTAGC
NsiI 4005 ATGCAT ATGCA^T
PacI 1188 TTAATTAA TTAAT^TAA
PflFI 2949 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2795 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1137 TCATGA T^CATGA
SalI 4103 GTCGAC G^TCGAC
SpeI 2917 ACTAGT A^CTAGT
Tth111I 2949 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Aor51HI 87 AGCGCT AGC^GCT
1232 AGCGCT
Asp700I 910 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1571 GAATGGATTC
BamHI 3316 GGATCC G^GATCC
4047 GGATCC
BanI 2005 GGTGCC G^G[CT][GA]CC
3234 GGTGCC
BbrPI 1708 CACGTG CAC^GTG
3724 CACGTG
BseRI 1663 CTCCTC GAGGAG(10/8)
3967 CTCCTC
Bsp1407I 3605 TGTACA T^GTACA
4156 TGTACA
BspMI 2218 ACCTGC ACCTGC(4/8)
4164 ACCTGC
BsrBI 2147 GAGCGG CCGCTC(-3/-3)
3241 GAGCGG
BsrDI 553 CATTGC GCAATG(2/0)
3329 GCAATG
BsrFI 447 GCCGGT [GA]^CCGG[CT]
3932 GCCGGC
BsrGI 3605 TGTACA T^GTACA
4156 TGTACA
BstAPI 766 GCATGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3804 GCATCTCCTGC
BstEII 827 GGTAACC G^GT[ACGT]ACC
3427 GGTTACC
BstPI 827 GGTAACC G^GT[ACGT]ACC
3427 GGTTACC
Cfr10I 447 GCCGGT [GA]^CCGG[CT]
3932 GCCGGC
DraII 2051 GGGCCCT [GA]G^G[ACGT]CC[CT]
2795 AGGACCT
Eco47III 87 AGCGCT AGC^GCT
1232 AGCGCT
EcoO109I 2051 GGGCCCT [GA]G^G[ACGT]CC[CT]
2795 AGGACCT
EcoO65I 827 GGTAACC G^GT[ACGT]ACC
3427 GGTTACC
FauI 807 CCCGC CCCGC(4/6)
1351 CCCGC
HindIII 2831 AAGCTT A^AGCTT
4131 AAGCTT
NdeI 788 CATATG CA^TATG
2937 CATATG
PmaCI 1708 CACGTG CAC^GTG
3724 CACGTG
PmlI 1708 CACGTG CAC^GTG
3724 CACGTG
SacI 3517 GAGCTC GAGCT^C
3751 GAGCTC
SapI 2144 GAAGAGC GCTCTTC(1/4)
4290 GCTCTTC
SbfI 3810 CCTGCAGG CCTGCA^GG
4165 CCTGCAGG
ScaI 1542 AGTACT AGT^ACT
2293 AGTACT
SphI 802 GCATGC GCATG^C
4003 GCATGC
Sse8387I 3810 CCTGCAGG CCTGCA^GG
4165 CCTGCAGG
SspBI 3605 TGTACA T^GTACA
4156 TGTACA
StuI 780 AGGCCT AGG^CCT
3335 AGGCCT
XcmI 652 CCAGCACCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3251 CCACTCTGGAAGTGG
XmnI 910 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1571 GAATGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BanII BbsI BbvI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI EarI EcoNI EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII