################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AgeI AscI AseI AsnI Asp718I AviII AvrII BalI BbeI BcgI BclI BglI BglII BlnI BsaBI BsaHI BsiEI BsiWI BsmBI BspDI BssHII BstBI BstUI ClaI CpoI DraIII DrdI EaeI EagI EclXI Eco52I EcoRV FbaI FseI FspI HgaI Hin1I KasI KpnI KspI MamI MfeI MluI MluNI MscI MunI MvnI NarI NotI NruI NspV PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SspI SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccIII 459 TCCGGA T^CCGGA AflII 526 CTTAAG C^TTAAG AhdI 2649 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1044 GTGCAC G^TGCAC AocI 4042 CCTTAGG CC^T[ACGT]AGG ApaI 2051 GGGCCC GGGCC^C ApaLI 1044 GTGCAC G^TGCAC AspEI 2649 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2949 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 3392 CTCGGG C^[CT]CG[GA]G BbvCI 3847 GCTGAGG CCTCAGC(-5/-2) BciVI 1769 GGATAC GTATCC(6/5) BcnI 2017 CCGGG CC^[GC]GG BfrI 526 CTTAAG C^TTAAG BsaWI 459 TCCGGA [AT]^CCGG[AT] BseAI 459 TCCGGA T^CCGGA BsgI 3647 CTGCAC GTGCAG(16/14) BsmI 4231 GCATTC GAATGC(1/-1) BsoBI 3392 CTCGGG C^[CT]CG[GA]G BspEI 459 TCCGGA T^CCGGA BspHI 1137 TCATGA T^CATGA BssSI 2352 CTCGTG CACGAG(-5/-1) Bst1107I 3486 GTATAC GTA^TAC BstXI 739 CCAGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 3486 GTATAC GTA^TAC Bsu36I 4042 CCTTAGG CC^T[ACGT]AGG DsaI 1201 CCATGG C^C[GA][CT]GG Eam1105I 2649 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 4042 CCTTAGG CC^T[ACGT]AGG EcoT22I 4005 ATGCAT ATGCA^T HpaI 4274 GTTAAC GTT^AAC MroI 459 TCCGGA T^CCGGA NaeI 3932 GCCGGC GCC^GGC NciI 2017 CCGGG CC^[GC]GG NcoI 1201 CCATGG C^CATGG NgoMIV 3932 GCCGGC G^CCGGC NheI 3944 GCTAGC G^CTAGC NsiI 4005 ATGCAT ATGCA^T PacI 1188 TTAATTAA TTAAT^TAA PflFI 2949 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 2795 AGGACCT [GA]G^G[AT]CC[CT] RcaI 1137 TCATGA T^CATGA SalI 4103 GTCGAC G^TCGAC SpeI 2917 ACTAGT A^CTAGT Tth111I 2949 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### Aor51HI 87 AGCGCT AGC^GCT 1232 AGCGCT Asp700I 910 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1571 GAATGGATTC BamHI 3316 GGATCC G^GATCC 4047 GGATCC BanI 2005 GGTGCC G^G[CT][GA]CC 3234 GGTGCC BbrPI 1708 CACGTG CAC^GTG 3724 CACGTG BseRI 1663 CTCCTC GAGGAG(10/8) 3967 CTCCTC Bsp1407I 3605 TGTACA T^GTACA 4156 TGTACA BspMI 2218 ACCTGC ACCTGC(4/8) 4164 ACCTGC BsrBI 2147 GAGCGG CCGCTC(-3/-3) 3241 GAGCGG BsrDI 553 CATTGC GCAATG(2/0) 3329 GCAATG BsrFI 447 GCCGGT [GA]^CCGG[CT] 3932 GCCGGC BsrGI 3605 TGTACA T^GTACA 4156 TGTACA BstAPI 766 GCATGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3804 GCATCTCCTGC BstEII 827 GGTAACC G^GT[ACGT]ACC 3427 GGTTACC BstPI 827 GGTAACC G^GT[ACGT]ACC 3427 GGTTACC Cfr10I 447 GCCGGT [GA]^CCGG[CT] 3932 GCCGGC DraII 2051 GGGCCCT [GA]G^G[ACGT]CC[CT] 2795 AGGACCT Eco47III 87 AGCGCT AGC^GCT 1232 AGCGCT EcoO109I 2051 GGGCCCT [GA]G^G[ACGT]CC[CT] 2795 AGGACCT EcoO65I 827 GGTAACC G^GT[ACGT]ACC 3427 GGTTACC FauI 807 CCCGC CCCGC(4/6) 1351 CCCGC HindIII 2831 AAGCTT A^AGCTT 4131 AAGCTT NdeI 788 CATATG CA^TATG 2937 CATATG PmaCI 1708 CACGTG CAC^GTG 3724 CACGTG PmlI 1708 CACGTG CAC^GTG 3724 CACGTG SacI 3517 GAGCTC GAGCT^C 3751 GAGCTC SapI 2144 GAAGAGC GCTCTTC(1/4) 4290 GCTCTTC SbfI 3810 CCTGCAGG CCTGCA^GG 4165 CCTGCAGG ScaI 1542 AGTACT AGT^ACT 2293 AGTACT SphI 802 GCATGC GCATG^C 4003 GCATGC Sse8387I 3810 CCTGCAGG CCTGCA^GG 4165 CCTGCAGG SspBI 3605 TGTACA T^GTACA 4156 TGTACA StuI 780 AGGCCT AGG^CCT 3335 AGGCCT XcmI 652 CCAGCACCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3251 CCACTCTGGAAGTGG XmnI 910 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1571 GAATGGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BanII BbsI BbvI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI EarI EcoNI EcoRI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII