################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII AccIII AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BamHI BcgI BfrI BlnI BsaBI BseAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BstBI Bsu36I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoT22I FseI FspI HindIII HpaI KspI MamI MfeI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI PvuI RsrII SacII SalI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AclI 1945 AACGTT AA^CGTT Alw44I 2058 GTGCAC G^TGCAC ApaLI 2058 GTGCAC G^TGCAC BbrPI 1560 CACGTG CAC^GTG BciVI 2144 GTATCC GTATCC(6/5) BglI 2244 GCCCTCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 1560 CACGTG [CT]AC^GT[GA] BsaI 1686 GAGACC GGTCTC(1/5) BsaWI 979 ACCGGA [AT]^CCGG[AT] BsiEI 1673 CGACCG CG[GA][CT]^CG BsmI 1535 GCATTC GAATGC(1/-1) BsrBI 2284 GAGCGG CCGCTC(-3/-3) BsrFI 1457 GCCGGC [GA]^CCGG[CT] Bst1107I 1848 GTATAC GTA^TAC BstEII 1091 GGTCACC G^GT[ACGT]ACC BstPI 1091 GGTCACC G^GT[ACGT]ACC BstZ17I 1848 GTATAC GTA^TAC Cfr10I 1457 GCCGGC [GA]^CCGG[CT] DrdI 1407 GACTCAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 2776 CCATGG C^C[GA][CT]GG EcoO65I 1091 GGTCACC G^GT[ACGT]ACC HincII 1977 GTCAAC GT[CT]^[GA]AC HindII 1977 GTCAAC GT[CT]^[GA]AC MluI 779 ACGCGT A^CGCGT NaeI 1457 GCCGGC GCC^GGC NcoI 2776 CCATGG C^CATGG NgoMIV 1457 GCCGGC G^CCGGC PmaCI 1560 CACGTG CAC^GTG PmlI 1560 CACGTG CAC^GTG PshAI 3187 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1945 AACGTT AA^CGTT SapI 1928 GAAGAGC GCTCTTC(1/4) ScaI 305 AGTACT AGT^ACT SexAI 1972 ACCAGGT A^CC[AT]GGT SphI 3106 GCATGC GCATG^C SwaI 2875 ATTTAAAT ATTT^AAAT ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Acc65I 592 GGTACC G^GTACC 976 GGTACC AccI 945 GTAGAC GT^[AC][GT]AC 1848 GTATAC AccII 780 CGCG CG^CG 1442 CGCG AflIII 779 ACGCGT A^C[GA][CT]GT 835 ACGTGT ApaI 479 GGGCCC GGGCC^C 2438 GGGCCC Asp718I 592 GGTACC G^GTACC 976 GGTACC BbeI 1454 GGCGCC GGCGC^C 1503 GGCGCC BclI 28 TGATCA T^GATCA 934 TGATCA BspMI 2140 GCAGGT ACCTGC(4/8) 2647 GCAGGT BstAPI 311 GCAGTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1184 GCAGAAGCTGC BstUI 780 CGCG CG^CG 1442 CGCG BstXI 964 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2272 CCAATGCTATGG DraI 2876 TTTAAA TTT^AAA 3904 TTTAAA DraIII 2351 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG 4201 CACATGGTG EcoRV 191 GATATC GAT^ATC 3053 GATATC FbaI 28 TGATCA T^GATCA 934 TGATCA KasI 1454 GGCGCC G^GCGCC 1503 GGCGCC KpnI 592 GGTACC GGTAC^C 976 GGTACC MvnI 780 CGCG CG^CG 1442 CGCG NarI 1454 GGCGCC GG^CGCC 1503 GGCGCC NspI 3106 GCATGC [GA]CATG^[CT] 3733 GCATGT PflMI 1001 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2350 CCACGCAGTGG SfoI 1454 GGCGCC GGC^GCC 1503 GGCGCC SmlI 2642 CTTGAG C^T[CT][GA]AG 3320 CTTGAG StuI 371 AGGCCT AGG^CCT 3787 AGGCCT Van91I 1001 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2350 CCACGCAGTGG XbaI 3741 TCTAGA T^CTAGA 3768 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrDI BsrI BssKI BssSI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI