##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
AspEI AspI AvaI AviII AvrII
BamHI BcgI BfrI BlnI BsaBI
BseAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
BstBI Bsu36I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoT22I FseI FspI HindIII
HpaI KspI MamI MfeI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI PvuI RsrII
SacII SalI SbfI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AclI 1945 AACGTT AA^CGTT
Alw44I 2058 GTGCAC G^TGCAC
ApaLI 2058 GTGCAC G^TGCAC
BbrPI 1560 CACGTG CAC^GTG
BciVI 2144 GTATCC GTATCC(6/5)
BglI 2244 GCCCTCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1560 CACGTG [CT]AC^GT[GA]
BsaI 1686 GAGACC GGTCTC(1/5)
BsaWI 979 ACCGGA [AT]^CCGG[AT]
BsiEI 1673 CGACCG CG[GA][CT]^CG
BsmI 1535 GCATTC GAATGC(1/-1)
BsrBI 2284 GAGCGG CCGCTC(-3/-3)
BsrFI 1457 GCCGGC [GA]^CCGG[CT]
Bst1107I 1848 GTATAC GTA^TAC
BstEII 1091 GGTCACC G^GT[ACGT]ACC
BstPI 1091 GGTCACC G^GT[ACGT]ACC
BstZ17I 1848 GTATAC GTA^TAC
Cfr10I 1457 GCCGGC [GA]^CCGG[CT]
DrdI 1407 GACTCAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2776 CCATGG C^C[GA][CT]GG
EcoO65I 1091 GGTCACC G^GT[ACGT]ACC
HincII 1977 GTCAAC GT[CT]^[GA]AC
HindII 1977 GTCAAC GT[CT]^[GA]AC
MluI 779 ACGCGT A^CGCGT
NaeI 1457 GCCGGC GCC^GGC
NcoI 2776 CCATGG C^CATGG
NgoMIV 1457 GCCGGC G^CCGGC
PmaCI 1560 CACGTG CAC^GTG
PmlI 1560 CACGTG CAC^GTG
PshAI 3187 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1945 AACGTT AA^CGTT
SapI 1928 GAAGAGC GCTCTTC(1/4)
ScaI 305 AGTACT AGT^ACT
SexAI 1972 ACCAGGT A^CC[AT]GGT
SphI 3106 GCATGC GCATG^C
SwaI 2875 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 592 GGTACC G^GTACC
976 GGTACC
AccI 945 GTAGAC GT^[AC][GT]AC
1848 GTATAC
AccII 780 CGCG CG^CG
1442 CGCG
AflIII 779 ACGCGT A^C[GA][CT]GT
835 ACGTGT
ApaI 479 GGGCCC GGGCC^C
2438 GGGCCC
Asp718I 592 GGTACC G^GTACC
976 GGTACC
BbeI 1454 GGCGCC GGCGC^C
1503 GGCGCC
BclI 28 TGATCA T^GATCA
934 TGATCA
BspMI 2140 GCAGGT ACCTGC(4/8)
2647 GCAGGT
BstAPI 311 GCAGTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1184 GCAGAAGCTGC
BstUI 780 CGCG CG^CG
1442 CGCG
BstXI 964 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2272 CCAATGCTATGG
DraI 2876 TTTAAA TTT^AAA
3904 TTTAAA
DraIII 2351 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4201 CACATGGTG
EcoRV 191 GATATC GAT^ATC
3053 GATATC
FbaI 28 TGATCA T^GATCA
934 TGATCA
KasI 1454 GGCGCC G^GCGCC
1503 GGCGCC
KpnI 592 GGTACC GGTAC^C
976 GGTACC
MvnI 780 CGCG CG^CG
1442 CGCG
NarI 1454 GGCGCC GG^CGCC
1503 GGCGCC
NspI 3106 GCATGC [GA]CATG^[CT]
3733 GCATGT
PflMI 1001 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2350 CCACGCAGTGG
SfoI 1454 GGCGCC GGC^GCC
1503 GGCGCC
SmlI 2642 CTTGAG C^T[CT][GA]AG
3320 CTTGAG
StuI 371 AGGCCT AGG^CCT
3787 AGGCCT
Van91I 1001 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2350 CCACGCAGTGG
XbaI 3741 TCTAGA T^CTAGA
3768 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BsrDI BsrI BssKI BssSI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI