##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccI AccII AcyI AflII
AgeI Aor51HI AscI AviII BbeI
BbvCI BfrI BsaBI BsaHI BsiWI
BsmBI BspDI BspHI BspLU11I BsrDI
BssHII Bst1107I BstBI BstUI BstZ17I
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoRV FauI FseI FspI
Hin1I HindIII HpaI KasI KspI
MamI MfeI MluI MunI MvnI
NarI NotI NruI NspV PacI
PinAI PmeI PvuI RcaI RsrII
SacII SalI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccIII 2178 TCCGGA T^CCGGA
AclI 183 AACGTT AA^CGTT
Alw44I 2208 GTGCAC G^TGCAC
ApaLI 2208 GTGCAC G^TGCAC
AseI 2120 ATTAAT AT^TAAT
AsnI 2120 ATTAAT AT^TAAT
Asp700I 2278 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1466 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3243 GGATCC G^GATCC
BbrPI 1076 CACGTG CAC^GTG
BcgI 33 CGAGATTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2510 GGATAC GTATCC(6/5)
BcnI 2274 CCCGG CC^[GC]GG
BglII 864 AGATCT A^GATCT
BsaI 3680 GAGACC GGTCTC(1/5)
BseAI 2178 TCCGGA T^CCGGA
BsiEI 525 CGACCG CG[GA][CT]^CG
Bsp1407I 1419 TGTACA T^GTACA
BspEI 2178 TCCGGA T^CCGGA
BsrBI 1834 CCGCTC CCGCTC(-3/-3)
BsrGI 1419 TGTACA T^GTACA
BssSI 3368 CTCGTG CACGAG(-5/-1)
BstEII 2368 GGTCACC G^GT[ACGT]ACC
BstPI 2368 GGTCACC G^GT[ACGT]ACC
DraIII 1368 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1789 GACTGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4308 CCTGGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2368 GGTCACC G^GT[ACGT]ACC
EcoRI 1187 GAATTC G^AATTC
HaeII 998 AGCGCC [GA]GCGC^[CT]
HgaI 2969 GCGTC GACGC(5/10)
HincII 4294 GTCAAC GT[CT]^[GA]AC
HindII 4294 GTCAAC GT[CT]^[GA]AC
MroI 2178 TCCGGA T^CCGGA
NaeI 84 GCCGGC GCC^GGC
NciI 2274 CCCGG CC^[GC]GG
NdeI 3631 CATATG CA^TATG
NgoMIV 84 GCCGGC G^CCGGC
NheI 143 GCTAGC G^CTAGC
PaeR7I 31 CTCGAG C^TCGAG
PflFI 1466 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1076 CACGTG CAC^GTG
PmlI 1076 CACGTG CAC^GTG
PshAI 4218 GACATAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2120 ATTAAT AT^TAAT
Psp1406I 183 AACGTT AA^CGTT
SacI 1577 GAGCTC GAGCT^C
SbfI 3047 CCTGCAGG CCTGCA^GG
ScaI 1941 AGTACT AGT^ACT
Sse8387I 3047 CCTGCAGG CCTGCA^GG
SspBI 1419 TGTACA T^GTACA
Tth111I 1466 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 361 TCTAGA T^CTAGA
XhoI 31 CTCGAG C^TCGAG
XmnI 2278 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 322 ACGTGT A^C[GA][CT]GT
3991 ACACGT
AocI 65 CCTAAGG CC^T[ACGT]AGG
3461 CCTGAGG
ApaI 2386 GGGCCC GGGCC^C
2477 GGGCCC
AvaI 31 CTCGAG C^[CT]CG[GA]G
2031 CTCGGG
BclI 1807 TGATCA T^GATCA
4551 TGATCA
BsaAI 1076 CACGTG [CT]AC^GT[GA]
3992 CACGTA
BseRI 884 CTCCTC GAGGAG(10/8)
2802 CTCCTC
BsmI 841 GAATGC GAATGC(1/-1)
1483 GAATGC
BsoBI 31 CTCGAG C^[CT]CG[GA]G
2031 CTCGGG
BsrFI 84 GCCGGC [GA]^CCGG[CT]
1602 ACCGGC
BstAPI 845 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4191 GCAGTGCATGC
BstXI 1173 CCAGTCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1893 CCAATGACCTGG
BstYI 864 AGATCT [GA]^GATC[CT]
3243 GGATCC
Bsu36I 65 CCTAAGG CC^T[ACGT]AGG
3461 CCTGAGG
Cfr10I 84 GCCGGC [GA]^CCGG[CT]
1602 ACCGGC
DraI 3117 TTTAAA TTT^AAA
4075 TTTAAA
Eco81I 65 CCTAAGG CC^T[ACGT]AGG
3461 CCTGAGG
EcoT22I 3638 ATGCAT ATGCA^T
4198 ATGCAT
FbaI 1807 TGATCA T^GATCA
4551 TGATCA
MflI 864 AGATCT [GA]^GATC[CT]
3243 GGATCC
NsiI 3638 ATGCAT ATGCA^T
4198 ATGCAT
PvuII 1559 CAGCTG CAG^CTG
1997 CAGCTG
SspI 3918 AATATT AAT^ATT
4067 AATATT
XcmI 1170 CCACCAGTCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2113 CCAGATCATTAATGG
XhoII 864 AGATCT [GA]^GATC[CT]
3243 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AhdI
AluI AlwI AlwNI ApoI Asp718I
AspEI AspHI AvaII AvrII BalI
BanI BanII BbsI BbvI BfaI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BsaWI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I