##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

AatII       AccI        AccII       AcyI        AflII       
AgeI        Aor51HI     AscI        AviII       BbeI        
BbvCI       BfrI        BsaBI       BsaHI       BsiWI       
BsmBI       BspDI       BspHI       BspLU11I    BsrDI       
BssHII      Bst1107I    BstBI       BstUI       BstZ17I     
ClaI        CpoI        EagI        EclXI       Eco47III    
Eco52I      EcoRV       FauI        FseI        FspI        
Hin1I       HindIII     HpaI        KasI        KspI        
MamI        MfeI        MluI        MunI        MvnI        
NarI        NotI        NruI        NspV        PacI        
PinAI       PmeI        PvuI        RcaI        RsrII       
SacII       SalI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccIII       2178  TCCGGA               T^CCGGA
AclI          183  AACGTT               AA^CGTT
Alw44I       2208  GTGCAC               G^TGCAC
ApaLI        2208  GTGCAC               G^TGCAC
AseI         2120  ATTAAT               AT^TAAT
AsnI         2120  ATTAAT               AT^TAAT
Asp700I      2278  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1466  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        3243  GGATCC               G^GATCC
BbrPI        1076  CACGTG               CAC^GTG
BcgI           33  CGAGATTTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2510  GGATAC               GTATCC(6/5)
BcnI         2274  CCCGG                CC^[GC]GG
BglII         864  AGATCT               A^GATCT
BsaI         3680  GAGACC               GGTCTC(1/5)
BseAI        2178  TCCGGA               T^CCGGA
BsiEI         525  CGACCG               CG[GA][CT]^CG
Bsp1407I     1419  TGTACA               T^GTACA
BspEI        2178  TCCGGA               T^CCGGA
BsrBI        1834  CCGCTC               CCGCTC(-3/-3)
BsrGI        1419  TGTACA               T^GTACA
BssSI        3368  CTCGTG               CACGAG(-5/-1)
BstEII       2368  GGTCACC              G^GT[ACGT]ACC
BstPI        2368  GGTCACC              G^GT[ACGT]ACC
DraIII       1368  CACGGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1789  GACTGCCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        4308  CCTGGGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      2368  GGTCACC              G^GT[ACGT]ACC
EcoRI        1187  GAATTC               G^AATTC
HaeII         998  AGCGCC               [GA]GCGC^[CT]
HgaI         2969  GCGTC                GACGC(5/10)
HincII       4294  GTCAAC               GT[CT]^[GA]AC
HindII       4294  GTCAAC               GT[CT]^[GA]AC
MroI         2178  TCCGGA               T^CCGGA
NaeI           84  GCCGGC               GCC^GGC
NciI         2274  CCCGG                CC^[GC]GG
NdeI         3631  CATATG               CA^TATG
NgoMIV         84  GCCGGC               G^CCGGC
NheI          143  GCTAGC               G^CTAGC
PaeR7I         31  CTCGAG               C^TCGAG
PflFI        1466  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1076  CACGTG               CAC^GTG
PmlI         1076  CACGTG               CAC^GTG
PshAI        4218  GACATAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        2120  ATTAAT               AT^TAAT
Psp1406I      183  AACGTT               AA^CGTT
SacI         1577  GAGCTC               GAGCT^C
SbfI         3047  CCTGCAGG             CCTGCA^GG
ScaI         1941  AGTACT               AGT^ACT
Sse8387I     3047  CCTGCAGG             CCTGCA^GG
SspBI        1419  TGTACA               T^GTACA
Tth111I      1466  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          361  TCTAGA               T^CTAGA
XhoI           31  CTCGAG               C^TCGAG
XmnI         2278  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AflIII        322  ACGTGT               A^C[GA][CT]GT
             3991  ACACGT              
AocI           65  CCTAAGG              CC^T[ACGT]AGG
             3461  CCTGAGG             
ApaI         2386  GGGCCC               GGGCC^C
             2477  GGGCCC              
AvaI           31  CTCGAG               C^[CT]CG[GA]G
             2031  CTCGGG              
BclI         1807  TGATCA               T^GATCA
             4551  TGATCA              
BsaAI        1076  CACGTG               [CT]AC^GT[GA]
             3992  CACGTA              
BseRI         884  CTCCTC               GAGGAG(10/8)
             2802  CTCCTC              
BsmI          841  GAATGC               GAATGC(1/-1)
             1483  GAATGC              
BsoBI          31  CTCGAG               C^[CT]CG[GA]G
             2031  CTCGGG              
BsrFI          84  GCCGGC               [GA]^CCGG[CT]
             1602  ACCGGC              
BstAPI        845  GCAGACCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4191  GCAGTGCATGC         
BstXI        1173  CCAGTCCGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1893  CCAATGACCTGG        
BstYI         864  AGATCT               [GA]^GATC[CT]
             3243  GGATCC              
Bsu36I         65  CCTAAGG              CC^T[ACGT]AGG
             3461  CCTGAGG             
Cfr10I         84  GCCGGC               [GA]^CCGG[CT]
             1602  ACCGGC              
DraI         3117  TTTAAA               TTT^AAA
             4075  TTTAAA              
Eco81I         65  CCTAAGG              CC^T[ACGT]AGG
             3461  CCTGAGG             
EcoT22I      3638  ATGCAT               ATGCA^T
             4198  ATGCAT              
FbaI         1807  TGATCA               T^GATCA
             4551  TGATCA              
MflI          864  AGATCT               [GA]^GATC[CT]
             3243  GGATCC              
NsiI         3638  ATGCAT               ATGCA^T
             4198  ATGCAT              
PvuII        1559  CAGCTG               CAG^CTG
             1997  CAGCTG              
SspI         3918  AATATT               AAT^ATT
             4067  AATATT              
XcmI         1170  CCACCAGTCCGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2113  CCAGATCATTAATGG     
XhoII         864  AGATCT               [GA]^GATC[CT]
             3243  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

Acc65I      AciI        AcsI        AfaI        AhdI        
AluI        AlwI        AlwNI       ApoI        Asp718I     
AspEI       AspHI       AvaII       AvrII       BalI        
BanI        BanII       BbsI        BbvI        BfaI        
BglI        BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaJI       BsaWI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
Eam1105I    EarI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
KpnI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SphI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I