################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII AccI AccII AcyI AflII AgeI Aor51HI AscI AviII BbeI BbvCI BfrI BsaBI BsaHI BsiWI BsmBI BspDI BspHI BspLU11I BsrDI BssHII Bst1107I BstBI BstUI BstZ17I ClaI CpoI EagI EclXI Eco47III Eco52I EcoRV FauI FseI FspI Hin1I HindIII HpaI KasI KspI MamI MfeI MluI MunI MvnI NarI NotI NruI NspV PacI PinAI PmeI PvuI RcaI RsrII SacII SalI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccIII 2178 TCCGGA T^CCGGA AclI 183 AACGTT AA^CGTT Alw44I 2208 GTGCAC G^TGCAC ApaLI 2208 GTGCAC G^TGCAC AseI 2120 ATTAAT AT^TAAT AsnI 2120 ATTAAT AT^TAAT Asp700I 2278 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1466 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 3243 GGATCC G^GATCC BbrPI 1076 CACGTG CAC^GTG BcgI 33 CGAGATTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2510 GGATAC GTATCC(6/5) BcnI 2274 CCCGG CC^[GC]GG BglII 864 AGATCT A^GATCT BsaI 3680 GAGACC GGTCTC(1/5) BseAI 2178 TCCGGA T^CCGGA BsiEI 525 CGACCG CG[GA][CT]^CG Bsp1407I 1419 TGTACA T^GTACA BspEI 2178 TCCGGA T^CCGGA BsrBI 1834 CCGCTC CCGCTC(-3/-3) BsrGI 1419 TGTACA T^GTACA BssSI 3368 CTCGTG CACGAG(-5/-1) BstEII 2368 GGTCACC G^GT[ACGT]ACC BstPI 2368 GGTCACC G^GT[ACGT]ACC DraIII 1368 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1789 GACTGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 4308 CCTGGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2368 GGTCACC G^GT[ACGT]ACC EcoRI 1187 GAATTC G^AATTC HaeII 998 AGCGCC [GA]GCGC^[CT] HgaI 2969 GCGTC GACGC(5/10) HincII 4294 GTCAAC GT[CT]^[GA]AC HindII 4294 GTCAAC GT[CT]^[GA]AC MroI 2178 TCCGGA T^CCGGA NaeI 84 GCCGGC GCC^GGC NciI 2274 CCCGG CC^[GC]GG NdeI 3631 CATATG CA^TATG NgoMIV 84 GCCGGC G^CCGGC NheI 143 GCTAGC G^CTAGC PaeR7I 31 CTCGAG C^TCGAG PflFI 1466 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1076 CACGTG CAC^GTG PmlI 1076 CACGTG CAC^GTG PshAI 4218 GACATAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2120 ATTAAT AT^TAAT Psp1406I 183 AACGTT AA^CGTT SacI 1577 GAGCTC GAGCT^C SbfI 3047 CCTGCAGG CCTGCA^GG ScaI 1941 AGTACT AGT^ACT Sse8387I 3047 CCTGCAGG CCTGCA^GG SspBI 1419 TGTACA T^GTACA Tth111I 1466 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 361 TCTAGA T^CTAGA XhoI 31 CTCGAG C^TCGAG XmnI 2278 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AflIII 322 ACGTGT A^C[GA][CT]GT 3991 ACACGT AocI 65 CCTAAGG CC^T[ACGT]AGG 3461 CCTGAGG ApaI 2386 GGGCCC GGGCC^C 2477 GGGCCC AvaI 31 CTCGAG C^[CT]CG[GA]G 2031 CTCGGG BclI 1807 TGATCA T^GATCA 4551 TGATCA BsaAI 1076 CACGTG [CT]AC^GT[GA] 3992 CACGTA BseRI 884 CTCCTC GAGGAG(10/8) 2802 CTCCTC BsmI 841 GAATGC GAATGC(1/-1) 1483 GAATGC BsoBI 31 CTCGAG C^[CT]CG[GA]G 2031 CTCGGG BsrFI 84 GCCGGC [GA]^CCGG[CT] 1602 ACCGGC BstAPI 845 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4191 GCAGTGCATGC BstXI 1173 CCAGTCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1893 CCAATGACCTGG BstYI 864 AGATCT [GA]^GATC[CT] 3243 GGATCC Bsu36I 65 CCTAAGG CC^T[ACGT]AGG 3461 CCTGAGG Cfr10I 84 GCCGGC [GA]^CCGG[CT] 1602 ACCGGC DraI 3117 TTTAAA TTT^AAA 4075 TTTAAA Eco81I 65 CCTAAGG CC^T[ACGT]AGG 3461 CCTGAGG EcoT22I 3638 ATGCAT ATGCA^T 4198 ATGCAT FbaI 1807 TGATCA T^GATCA 4551 TGATCA MflI 864 AGATCT [GA]^GATC[CT] 3243 GGATCC NsiI 3638 ATGCAT ATGCA^T 4198 ATGCAT PvuII 1559 CAGCTG CAG^CTG 1997 CAGCTG SspI 3918 AATATT AAT^ATT 4067 AATATT XcmI 1170 CCACCAGTCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2113 CCAGATCATTAATGG XhoII 864 AGATCT [GA]^GATC[CT] 3243 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### Acc65I AciI AcsI AfaI AhdI AluI AlwI AlwNI ApoI Asp718I AspEI AspHI AvaII AvrII BalI BanI BanII BbsI BbvI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I