################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## Acc65I AccII AclI AflIII AgeI Aor51HI ApaI AscI AseI AsnI Asp718I AviII AvrII BbeI BbrPI BglI BglII BlnI BlpI Bpu1102I BsiWI BspDI BspLU11I BssHII Bst1107I BstBI BstUI BstZ17I CelII ClaI CpoI DraI Eco47III EcoRV EcoT22I FseI FspI HaeII HpaI KasI KpnI KspI MfeI MluI MunI MvnI NarI NcoI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AatII 3822 GACGTC GACGT^C AccIII 3726 TCCGGA T^CCGGA AflII 4032 CTTAAG C^TTAAG AhdI 3287 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 4211 GTGCAC G^TGCAC AocI 1243 CCTGAGG CC^T[ACGT]AGG ApaLI 4211 GTGCAC G^TGCAC AspEI 3287 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 2405 TGGCCA TGG^CCA BamHI 799 GGATCC G^GATCC BcgI 2492 GCACAGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3737 GGATAC GTATCC(6/5) BclI 486 TGATCA T^GATCA BfrI 4032 CTTAAG C^TTAAG BsaAI 2965 TACGTG [CT]AC^GT[GA] BsaBI 2263 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1675 GGTCTC GGTCTC(1/5) BseAI 3726 TCCGGA T^CCGGA BsiEI 3271 CGGCCG CG[GA][CT]^CG BsmBI 2927 CGTCTC CGTCTC(1/5) Bsp1407I 3005 TGTACA T^GTACA BspEI 3726 TCCGGA T^CCGGA BspHI 483 TCATGA T^CATGA BsrBI 2563 CCGCTC CCGCTC(-3/-3) BsrGI 3005 TGTACA T^GTACA BstAPI 2904 GCAGGTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1451 GGTAACC G^GT[ACGT]ACC BstPI 1451 GGTAACC G^GT[ACGT]ACC Bsu36I 1243 CCTGAGG CC^T[ACGT]AGG CfoI 2704 GCGC GCG^C DrdI 1667 GACTTCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 3271 CGGCCG C^GGCCG Eam1105I 3287 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 3271 CGGCCG C^GGCCG Eco52I 3271 CGGCCG C^GGCCG Eco81I 1243 CCTGAGG CC^T[ACGT]AGG EcoO65I 1451 GGTAACC G^GT[ACGT]ACC FbaI 486 TGATCA T^GATCA HhaI 2704 GCGC GCG^C HinP1I 2704 GCGC G^CGC HincII 2830 GTTGAC GT[CT]^[GA]AC HindII 2830 GTTGAC GT[CT]^[GA]AC MamI 2263 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2405 TGGCCA TGG^CCA MroI 3726 TCCGGA T^CCGGA MscI 2405 TGGCCA TGG^CCA NaeI 2683 GCCGGC GCC^GGC NdeI 582 CATATG CA^TATG NgoMIV 2683 GCCGGC G^CCGGC NheI 369 GCTAGC G^CTAGC PflMI 4317 CCAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 632 CAGCTG CAG^CTG RcaI 483 TCATGA T^CATGA SapI 692 GAAGAGC GCTCTTC(1/4) SexAI 4071 ACCAGGT A^CC[AT]GGT SphI 462 GCATGC GCATG^C SspBI 3005 TGTACA T^GTACA Van91I 4317 CCAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 577 GTAGAC GT^[AC][GT]AC 1874 GTCTAC AvaI 1256 CCCGGG C^[CT]CG[GA]G 3054 CCCGGG BbvCI 1190 GCTGAGG CCTCAGC(-5/-2) 1349 GCTGAGG BsaWI 3163 ACCGGA [AT]^CCGG[AT] 3726 TCCGGA BsmI 3810 GAATGC GAATGC(1/-1) 3570 GCATTC BsoBI 1256 CCCGGG C^[CT]CG[GA]G 3054 CCCGGG BsrDI 1939 CATTGC GCAATG(2/0) 3713 GCAATG BstXI 980 CCACAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3869 CCATGTGTCTGG DsaI 3411 CCACGG C^C[GA][CT]GG 3733 CCGTGG EcoT14I 2105 CCAAGG C^C[AT][AT]GG 4304 CCTTGG FauI 1822 CCCGC CCCGC(4/6) 2562 CCCGC HindIII 1553 AAGCTT A^AGCTT 2242 AAGCTT NspI 462 GCATGC [GA]CATG^[CT] 1736 GCATGT SmaI 1256 CCCGGG CCC^GGG 3054 CCCGGG StuI 1890 AGGCCT AGG^CCT 2108 AGGCCT StyI 2105 CCAAGG C^C[AT][AT]GG 4304 CCTTGG XmaCI 1256 CCCGGG C^CCGGG 3054 CCCGGG XmaI 1256 CCCGGG C^CCGGG 3054 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AspI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraII DraIII EaeI EarI EcoNI EcoO109I EcoRI EcoRII Fnu4HI FokI HaeIII HapII HgaI Hin1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflFI PleI PpuMI PshAI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XbaI XcmI XhoII XmnI