##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

Acc65I      AccII       AclI        AflIII      AgeI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AviII       AvrII       BbeI        BbrPI       
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsiWI       BspDI       BspLU11I    BssHII      Bst1107I    
BstBI       BstUI       BstZ17I     CelII       ClaI        
CpoI        DraI        Eco47III    EcoRV       EcoT22I     
FseI        FspI        HaeII       HpaI        KasI        
KpnI        KspI        MfeI        MluI        MunI        
MvnI        NarI        NcoI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspI        
SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AatII        3822  GACGTC               GACGT^C
AccIII       3726  TCCGGA               T^CCGGA
AflII        4032  CTTAAG               C^TTAAG
AhdI         3287  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       4211  GTGCAC               G^TGCAC
AocI         1243  CCTGAGG              CC^T[ACGT]AGG
ApaLI        4211  GTGCAC               G^TGCAC
AspEI        3287  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         2405  TGGCCA               TGG^CCA
BamHI         799  GGATCC               G^GATCC
BcgI         2492  GCACAGACCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3737  GGATAC               GTATCC(6/5)
BclI          486  TGATCA               T^GATCA
BfrI         4032  CTTAAG               C^TTAAG
BsaAI        2965  TACGTG               [CT]AC^GT[GA]
BsaBI        2263  GATGGCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1675  GGTCTC               GGTCTC(1/5)
BseAI        3726  TCCGGA               T^CCGGA
BsiEI        3271  CGGCCG               CG[GA][CT]^CG
BsmBI        2927  CGTCTC               CGTCTC(1/5)
Bsp1407I     3005  TGTACA               T^GTACA
BspEI        3726  TCCGGA               T^CCGGA
BspHI         483  TCATGA               T^CATGA
BsrBI        2563  CCGCTC               CCGCTC(-3/-3)
BsrGI        3005  TGTACA               T^GTACA
BstAPI       2904  GCAGGTAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1451  GGTAACC              G^GT[ACGT]ACC
BstPI        1451  GGTAACC              G^GT[ACGT]ACC
Bsu36I       1243  CCTGAGG              CC^T[ACGT]AGG
CfoI         2704  GCGC                 GCG^C
DrdI         1667  GACTTCCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         3271  CGGCCG               C^GGCCG
Eam1105I     3287  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        3271  CGGCCG               C^GGCCG
Eco52I       3271  CGGCCG               C^GGCCG
Eco81I       1243  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      1451  GGTAACC              G^GT[ACGT]ACC
FbaI          486  TGATCA               T^GATCA
HhaI         2704  GCGC                 GCG^C
HinP1I       2704  GCGC                 G^CGC
HincII       2830  GTTGAC               GT[CT]^[GA]AC
HindII       2830  GTTGAC               GT[CT]^[GA]AC
MamI         2263  GATGGCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        2405  TGGCCA               TGG^CCA
MroI         3726  TCCGGA               T^CCGGA
MscI         2405  TGGCCA               TGG^CCA
NaeI         2683  GCCGGC               GCC^GGC
NdeI          582  CATATG               CA^TATG
NgoMIV       2683  GCCGGC               G^CCGGC
NheI          369  GCTAGC               G^CTAGC
PflMI        4317  CCAAATGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         632  CAGCTG               CAG^CTG
RcaI          483  TCATGA               T^CATGA
SapI          692  GAAGAGC              GCTCTTC(1/4)
SexAI        4071  ACCAGGT              A^CC[AT]GGT
SphI          462  GCATGC               GCATG^C
SspBI        3005  TGTACA               T^GTACA
Van91I       4317  CCAAATGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccI          577  GTAGAC               GT^[AC][GT]AC
             1874  GTCTAC              
AvaI         1256  CCCGGG               C^[CT]CG[GA]G
             3054  CCCGGG              
BbvCI        1190  GCTGAGG              CCTCAGC(-5/-2)
             1349  GCTGAGG             
BsaWI        3163  ACCGGA               [AT]^CCGG[AT]
             3726  TCCGGA              
BsmI         3810  GAATGC               GAATGC(1/-1)
             3570  GCATTC              
BsoBI        1256  CCCGGG               C^[CT]CG[GA]G
             3054  CCCGGG              
BsrDI        1939  CATTGC               GCAATG(2/0)
             3713  GCAATG              
BstXI         980  CCACAGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3869  CCATGTGTCTGG        
DsaI         3411  CCACGG               C^C[GA][CT]GG
             3733  CCGTGG              
EcoT14I      2105  CCAAGG               C^C[AT][AT]GG
             4304  CCTTGG              
FauI         1822  CCCGC                CCCGC(4/6)
             2562  CCCGC               
HindIII      1553  AAGCTT               A^AGCTT
             2242  AAGCTT              
NspI          462  GCATGC               [GA]CATG^[CT]
             1736  GCATGT              
SmaI         1256  CCCGGG               CCC^GGG
             3054  CCCGGG              
StuI         1890  AGGCCT               AGG^CCT
             2108  AGGCCT              
StyI         2105  CCAAGG               C^C[AT][AT]GG
             4304  CCTTGG              
XmaCI        1256  CCCGGG               C^CCGGG
             3054  CCCGGG              
XmaI         1256  CCCGGG               C^CCGGG
             3054  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AspI        AvaII       BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaHI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstYI       Cac8I       
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      EaeI        EarI        EcoNI       EcoO109I    
EcoRI       EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HgaI        Hin1I       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflFI       PleI        PpuMI       PshAI       PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     XbaI        XcmI        XhoII       XmnI