##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AccII AclI AflIII AgeI
Aor51HI ApaI AscI AseI AsnI
Asp718I AviII AvrII BbeI BbrPI
BglI BglII BlnI BlpI Bpu1102I
BsiWI BspDI BspLU11I BssHII Bst1107I
BstBI BstUI BstZ17I CelII ClaI
CpoI DraI Eco47III EcoRV EcoT22I
FseI FspI HaeII HpaI KasI
KpnI KspI MfeI MluI MunI
MvnI NarI NcoI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 3822 GACGTC GACGT^C
AccIII 3726 TCCGGA T^CCGGA
AflII 4032 CTTAAG C^TTAAG
AhdI 3287 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4211 GTGCAC G^TGCAC
AocI 1243 CCTGAGG CC^T[ACGT]AGG
ApaLI 4211 GTGCAC G^TGCAC
AspEI 3287 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2405 TGGCCA TGG^CCA
BamHI 799 GGATCC G^GATCC
BcgI 2492 GCACAGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3737 GGATAC GTATCC(6/5)
BclI 486 TGATCA T^GATCA
BfrI 4032 CTTAAG C^TTAAG
BsaAI 2965 TACGTG [CT]AC^GT[GA]
BsaBI 2263 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1675 GGTCTC GGTCTC(1/5)
BseAI 3726 TCCGGA T^CCGGA
BsiEI 3271 CGGCCG CG[GA][CT]^CG
BsmBI 2927 CGTCTC CGTCTC(1/5)
Bsp1407I 3005 TGTACA T^GTACA
BspEI 3726 TCCGGA T^CCGGA
BspHI 483 TCATGA T^CATGA
BsrBI 2563 CCGCTC CCGCTC(-3/-3)
BsrGI 3005 TGTACA T^GTACA
BstAPI 2904 GCAGGTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1451 GGTAACC G^GT[ACGT]ACC
BstPI 1451 GGTAACC G^GT[ACGT]ACC
Bsu36I 1243 CCTGAGG CC^T[ACGT]AGG
CfoI 2704 GCGC GCG^C
DrdI 1667 GACTTCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3271 CGGCCG C^GGCCG
Eam1105I 3287 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3271 CGGCCG C^GGCCG
Eco52I 3271 CGGCCG C^GGCCG
Eco81I 1243 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1451 GGTAACC G^GT[ACGT]ACC
FbaI 486 TGATCA T^GATCA
HhaI 2704 GCGC GCG^C
HinP1I 2704 GCGC G^CGC
HincII 2830 GTTGAC GT[CT]^[GA]AC
HindII 2830 GTTGAC GT[CT]^[GA]AC
MamI 2263 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2405 TGGCCA TGG^CCA
MroI 3726 TCCGGA T^CCGGA
MscI 2405 TGGCCA TGG^CCA
NaeI 2683 GCCGGC GCC^GGC
NdeI 582 CATATG CA^TATG
NgoMIV 2683 GCCGGC G^CCGGC
NheI 369 GCTAGC G^CTAGC
PflMI 4317 CCAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 632 CAGCTG CAG^CTG
RcaI 483 TCATGA T^CATGA
SapI 692 GAAGAGC GCTCTTC(1/4)
SexAI 4071 ACCAGGT A^CC[AT]GGT
SphI 462 GCATGC GCATG^C
SspBI 3005 TGTACA T^GTACA
Van91I 4317 CCAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 577 GTAGAC GT^[AC][GT]AC
1874 GTCTAC
AvaI 1256 CCCGGG C^[CT]CG[GA]G
3054 CCCGGG
BbvCI 1190 GCTGAGG CCTCAGC(-5/-2)
1349 GCTGAGG
BsaWI 3163 ACCGGA [AT]^CCGG[AT]
3726 TCCGGA
BsmI 3810 GAATGC GAATGC(1/-1)
3570 GCATTC
BsoBI 1256 CCCGGG C^[CT]CG[GA]G
3054 CCCGGG
BsrDI 1939 CATTGC GCAATG(2/0)
3713 GCAATG
BstXI 980 CCACAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3869 CCATGTGTCTGG
DsaI 3411 CCACGG C^C[GA][CT]GG
3733 CCGTGG
EcoT14I 2105 CCAAGG C^C[AT][AT]GG
4304 CCTTGG
FauI 1822 CCCGC CCCGC(4/6)
2562 CCCGC
HindIII 1553 AAGCTT A^AGCTT
2242 AAGCTT
NspI 462 GCATGC [GA]CATG^[CT]
1736 GCATGT
SmaI 1256 CCCGGG CCC^GGG
3054 CCCGGG
StuI 1890 AGGCCT AGG^CCT
2108 AGGCCT
StyI 2105 CCAAGG C^C[AT][AT]GG
4304 CCTTGG
XmaCI 1256 CCCGGG C^CCGGG
3054 CCCGGG
XmaI 1256 CCCGGG C^CCGGG
3054 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AspI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstYI Cac8I
Cfr10I Cfr13I DdeI DpnII DraII
DraIII EaeI EarI EcoNI EcoO109I
EcoRI EcoRII Fnu4HI FokI HaeIII
HapII HgaI Hin1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflFI PleI PpuMI PshAI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XbaI XcmI XhoII XmnI