################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## Acc65I AccIII AgeI AhdI AocI ApaI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BciVI BlnI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BsrDI BssHII Bst1107I BstEII BstPI BstZ17I Bsu36I ClaI CpoI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRV FseI FspI HpaI KpnI KspI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI PaeR7I PinAI PmeI PshBI PvuI RsrII SacII SalI SbfI SexAI SgrAI SnaBI SpeI SphI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 1357 GACGTC GACGT^C AccII 2431 CGCG CG^CG AclI 3559 AACGTT AA^CGTT Alw44I 3128 GTGCAC G^TGCAC Aor51HI 2179 AGCGCT AGC^GCT ApaLI 3128 GTGCAC G^TGCAC BalI 379 TGGCCA TGG^CCA BbeI 1666 GGCGCC GGCGC^C BbvCI 23 GCTGAGG CCTCAGC(-5/-2) BclI 4385 TGATCA T^GATCA BglI 7 GCCAGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1066 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1066 GCTGAGC GC^T[ACGT]AGC BsaBI 3535 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 344 TCCGGT [AT]^CCGG[AT] Bsp1407I 493 TGTACA T^GTACA BspHI 1914 TCATGA T^CATGA BsrFI 674 ACCGGC [GA]^CCGG[CT] BsrGI 493 TGTACA T^GTACA BssSI 2129 CACGAG CACGAG(-5/-1) BstBI 2559 TTCGAA TT^CGAA BstUI 2431 CGCG CG^CG CelII 1066 GCTGAGC GC^T[ACGT]AGC Cfr10I 674 ACCGGC [GA]^CCGG[CT] Eco47III 2179 AGCGCT AGC^GCT EcoNI 1624 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 786 GAATTC G^AATTC EcoT22I 2930 ATGCAT ATGCA^T FbaI 4385 TGATCA T^GATCA KasI 1666 GGCGCC G^GCGCC MamI 3535 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 379 TGGCCA TGG^CCA MscI 379 TGGCCA TGG^CCA MvnI 2431 CGCG CG^CG NarI 1666 GGCGCC GG^CGCC NcoI 4214 CCATGG C^CATGG NsiI 2930 ATGCAT ATGCA^T NspV 2559 TTCGAA TT^CGAA PacI 701 TTAATTAA TTAAT^TAA PflMI 2833 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 4284 AGGACCC [GA]G^G[AT]CC[CT] PshAI 1532 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 3559 AACGTT AA^CGTT RcaI 1914 TCATGA T^CATGA ScaI 1076 AGTACT AGT^ACT SfiI 6 GGCCAGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1666 GGCGCC GGC^GCC SfuI 2559 TTCGAA TT^CGAA SmaI 1288 CCCGGG CCC^GGG SspBI 493 TGTACA T^GTACA SspI 4658 AATATT AAT^ATT Van91I 2833 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 3316 CCATGCAGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1288 CCCGGG C^CCGGG XmaI 1288 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 3975 GTCTAC GT^[AC][GT]AC 4272 GTCTAC AflIII 283 ACACGT A^C[GA][CT]GT 2145 ACGTGT Asp700I 3641 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4176 GAAAAGGTTC AspI 860 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2701 GACCTCGTC BbsI 1679 GAAGAC GAAGAC(2/6) 2446 GTCTTC BpuAI 1679 GAAGAC GAAGAC(2/6) 2446 GTCTTC BsgI 1598 GTGCAG GTGCAG(16/14) 2645 GTGCAG BsrBI 714 GAGCGG CCGCTC(-3/-3) 1742 GAGCGG BstAPI 1408 GCATGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1699 GCAAGACCTGC DraII 2775 AGGCCCT [GA]G^G[ACGT]CC[CT] 4284 AGGACCC DrdI 1370 GACAATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2429 GACGCGATGGTC DsaI 4214 CCATGG C^C[GA][CT]GG 4502 CCACGG EcoO109I 2775 AGGCCCT [GA]G^G[ACGT]CC[CT] 4284 AGGACCC HincII 2224 GTTGAC GT[CT]^[GA]AC 3604 GTCAAC HindII 2224 GTTGAC GT[CT]^[GA]AC 3604 GTCAAC NspI 361 GCATGT [GA]CATG^[CT] 3722 ACATGC PflFI 860 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2701 GACCTCGTC SacI 1432 GAGCTC GAGCT^C 2661 GAGCTC SapI 94 GAAGAGC GCTCTTC(1/4) 118 GAAGAGC StuI 588 AGGCCT AGG^CCT 2466 AGGCCT Tth111I 860 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2701 GACCTCGTC XmnI 3641 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4176 GAAAAGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AciI AcsI AcyI AfaI AflII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbrPI BbvI BcgI BcnI BfaI BfrI BglII BmyI BpmI BsaAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraIII EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PmaCI PmlI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII