##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccIII AgeI AhdI AocI
ApaI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BciVI
BlnI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspLU11I BsrDI BssHII
Bst1107I BstEII BstPI BstZ17I Bsu36I
ClaI CpoI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRV FseI
FspI HpaI KpnI KspI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI PaeR7I
PinAI PmeI PshBI PvuI RsrII
SacII SalI SbfI SexAI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 1357 GACGTC GACGT^C
AccII 2431 CGCG CG^CG
AclI 3559 AACGTT AA^CGTT
Alw44I 3128 GTGCAC G^TGCAC
Aor51HI 2179 AGCGCT AGC^GCT
ApaLI 3128 GTGCAC G^TGCAC
BalI 379 TGGCCA TGG^CCA
BbeI 1666 GGCGCC GGCGC^C
BbvCI 23 GCTGAGG CCTCAGC(-5/-2)
BclI 4385 TGATCA T^GATCA
BglI 7 GCCAGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1066 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1066 GCTGAGC GC^T[ACGT]AGC
BsaBI 3535 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 344 TCCGGT [AT]^CCGG[AT]
Bsp1407I 493 TGTACA T^GTACA
BspHI 1914 TCATGA T^CATGA
BsrFI 674 ACCGGC [GA]^CCGG[CT]
BsrGI 493 TGTACA T^GTACA
BssSI 2129 CACGAG CACGAG(-5/-1)
BstBI 2559 TTCGAA TT^CGAA
BstUI 2431 CGCG CG^CG
CelII 1066 GCTGAGC GC^T[ACGT]AGC
Cfr10I 674 ACCGGC [GA]^CCGG[CT]
Eco47III 2179 AGCGCT AGC^GCT
EcoNI 1624 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 786 GAATTC G^AATTC
EcoT22I 2930 ATGCAT ATGCA^T
FbaI 4385 TGATCA T^GATCA
KasI 1666 GGCGCC G^GCGCC
MamI 3535 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 379 TGGCCA TGG^CCA
MscI 379 TGGCCA TGG^CCA
MvnI 2431 CGCG CG^CG
NarI 1666 GGCGCC GG^CGCC
NcoI 4214 CCATGG C^CATGG
NsiI 2930 ATGCAT ATGCA^T
NspV 2559 TTCGAA TT^CGAA
PacI 701 TTAATTAA TTAAT^TAA
PflMI 2833 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 4284 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1532 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3559 AACGTT AA^CGTT
RcaI 1914 TCATGA T^CATGA
ScaI 1076 AGTACT AGT^ACT
SfiI 6 GGCCAGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1666 GGCGCC GGC^GCC
SfuI 2559 TTCGAA TT^CGAA
SmaI 1288 CCCGGG CCC^GGG
SspBI 493 TGTACA T^GTACA
SspI 4658 AATATT AAT^ATT
Van91I 2833 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3316 CCATGCAGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1288 CCCGGG C^CCGGG
XmaI 1288 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 3975 GTCTAC GT^[AC][GT]AC
4272 GTCTAC
AflIII 283 ACACGT A^C[GA][CT]GT
2145 ACGTGT
Asp700I 3641 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4176 GAAAAGGTTC
AspI 860 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2701 GACCTCGTC
BbsI 1679 GAAGAC GAAGAC(2/6)
2446 GTCTTC
BpuAI 1679 GAAGAC GAAGAC(2/6)
2446 GTCTTC
BsgI 1598 GTGCAG GTGCAG(16/14)
2645 GTGCAG
BsrBI 714 GAGCGG CCGCTC(-3/-3)
1742 GAGCGG
BstAPI 1408 GCATGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1699 GCAAGACCTGC
DraII 2775 AGGCCCT [GA]G^G[ACGT]CC[CT]
4284 AGGACCC
DrdI 1370 GACAATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2429 GACGCGATGGTC
DsaI 4214 CCATGG C^C[GA][CT]GG
4502 CCACGG
EcoO109I 2775 AGGCCCT [GA]G^G[ACGT]CC[CT]
4284 AGGACCC
HincII 2224 GTTGAC GT[CT]^[GA]AC
3604 GTCAAC
HindII 2224 GTTGAC GT[CT]^[GA]AC
3604 GTCAAC
NspI 361 GCATGT [GA]CATG^[CT]
3722 ACATGC
PflFI 860 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2701 GACCTCGTC
SacI 1432 GAGCTC GAGCT^C
2661 GAGCTC
SapI 94 GAAGAGC GCTCTTC(1/4)
118 GAAGAGC
StuI 588 AGGCCT AGG^CCT
2466 AGGCCT
Tth111I 860 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2701 GACCTCGTC
XmnI 3641 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4176 GAAAAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbrPI
BbvI BcgI BcnI BfaI BfrI
BglII BmyI BpmI BsaAI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraIII
EaeI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PmaCI PmlI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII