##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

Acc65I      AccIII      AgeI        AhdI        AocI        
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BamHI       BciVI       
BlnI        BseAI       BsiEI       BsiWI       BsmBI       
BspDI       BspEI       BspLU11I    BsrDI       BssHII      
Bst1107I    BstEII      BstPI       BstZ17I     Bsu36I      
ClaI        CpoI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoO65I     EcoRV       FseI        
FspI        HpaI        KpnI        KspI        MfeI        
MluI        MroI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        PaeR7I      
PinAI       PmeI        PshBI       PvuI        RsrII       
SacII       SalI        SbfI        SexAI       SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SwaI        
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AatII        1357  GACGTC               GACGT^C
AccII        2431  CGCG                 CG^CG
AclI         3559  AACGTT               AA^CGTT
Alw44I       3128  GTGCAC               G^TGCAC
Aor51HI      2179  AGCGCT               AGC^GCT
ApaLI        3128  GTGCAC               G^TGCAC
BalI          379  TGGCCA               TGG^CCA
BbeI         1666  GGCGCC               GGCGC^C
BbvCI          23  GCTGAGG              CCTCAGC(-5/-2)
BclI         4385  TGATCA               T^GATCA
BglI            7  GCCAGCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1066  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1066  GCTGAGC              GC^T[ACGT]AGC
BsaBI        3535  GATGAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         344  TCCGGT               [AT]^CCGG[AT]
Bsp1407I      493  TGTACA               T^GTACA
BspHI        1914  TCATGA               T^CATGA
BsrFI         674  ACCGGC               [GA]^CCGG[CT]
BsrGI         493  TGTACA               T^GTACA
BssSI        2129  CACGAG               CACGAG(-5/-1)
BstBI        2559  TTCGAA               TT^CGAA
BstUI        2431  CGCG                 CG^CG
CelII        1066  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        674  ACCGGC               [GA]^CCGG[CT]
Eco47III     2179  AGCGCT               AGC^GCT
EcoNI        1624  CCTCTCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         786  GAATTC               G^AATTC
EcoT22I      2930  ATGCAT               ATGCA^T
FbaI         4385  TGATCA               T^GATCA
KasI         1666  GGCGCC               G^GCGCC
MamI         3535  GATGAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         379  TGGCCA               TGG^CCA
MscI          379  TGGCCA               TGG^CCA
MvnI         2431  CGCG                 CG^CG
NarI         1666  GGCGCC               GG^CGCC
NcoI         4214  CCATGG               C^CATGG
NsiI         2930  ATGCAT               ATGCA^T
NspV         2559  TTCGAA               TT^CGAA
PacI          701  TTAATTAA             TTAAT^TAA
PflMI        2833  CCAGCAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        4284  AGGACCC              [GA]G^G[AT]CC[CT]
PshAI        1532  GACCTGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     3559  AACGTT               AA^CGTT
RcaI         1914  TCATGA               T^CATGA
ScaI         1076  AGTACT               AGT^ACT
SfiI            6  GGCCAGCGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1666  GGCGCC               GGC^GCC
SfuI         2559  TTCGAA               TT^CGAA
SmaI         1288  CCCGGG               CCC^GGG
SspBI         493  TGTACA               T^GTACA
SspI         4658  AATATT               AAT^ATT
Van91I       2833  CCAGCAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         3316  CCATGCAGTGAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1288  CCCGGG               C^CCGGG
XmaI         1288  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI         3975  GTCTAC               GT^[AC][GT]AC
             4272  GTCTAC              
AflIII        283  ACACGT               A^C[GA][CT]GT
             2145  ACGTGT              
Asp700I      3641  GAAAACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4176  GAAAAGGTTC          
AspI          860  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2701  GACCTCGTC           
BbsI         1679  GAAGAC               GAAGAC(2/6)
             2446  GTCTTC              
BpuAI        1679  GAAGAC               GAAGAC(2/6)
             2446  GTCTTC              
BsgI         1598  GTGCAG               GTGCAG(16/14)
             2645  GTGCAG              
BsrBI         714  GAGCGG               CCGCTC(-3/-3)
             1742  GAGCGG              
BstAPI       1408  GCATGATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1699  GCAAGACCTGC         
DraII        2775  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             4284  AGGACCC             
DrdI         1370  GACAATGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2429  GACGCGATGGTC        
DsaI         4214  CCATGG               C^C[GA][CT]GG
             4502  CCACGG              
EcoO109I     2775  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             4284  AGGACCC             
HincII       2224  GTTGAC               GT[CT]^[GA]AC
             3604  GTCAAC              
HindII       2224  GTTGAC               GT[CT]^[GA]AC
             3604  GTCAAC              
NspI          361  GCATGT               [GA]CATG^[CT]
             3722  ACATGC              
PflFI         860  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2701  GACCTCGTC           
SacI         1432  GAGCTC               GAGCT^C
             2661  GAGCTC              
SapI           94  GAAGAGC              GCTCTTC(1/4)
              118  GAAGAGC             
StuI          588  AGGCCT               AGG^CCT
             2466  AGGCCT              
Tth111I       860  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2701  GACCTCGTC           
XmnI         3641  GAAAACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4176  GAAAAGGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AflII       
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbrPI       
BbvI        BcgI        BcnI        BfaI        BfrI        
BglII       BmyI        BpmI        BsaAI       BsaHI       
BsaI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraIII      
EaeI        EarI        EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PmaCI       PmlI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII