################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII AclI AgeI ApaI AscI AseI AsnI AspI BamHI BbrPI BclI BlpI Bpu1102I BsiWI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstZ17I CelII CpoI DraIII EcoO65I EcoRV FbaI FseI HpaI KspI MluI NaeI NdeI NgoMIV NotI PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SbfI ScaI SfiI SgrAI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### Acc65I 3437 GGTACC G^GTACC AflIII 1478 ACATGT A^C[GA][CT]GT AhdI 3719 GACCGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2503 GTGCAC G^TGCAC AlwI 1011 GATCC GGATC(4/5) Aor51HI 1097 AGCGCT AGC^GCT ApaLI 2503 GTGCAC G^TGCAC Asp700I 3903 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 3437 GGTACC G^GTACC AspEI 3719 GACCGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2906 TGCGCA TGC^GCA AvrII 1188 CCTAGG C^CTAGG BalI 1878 TGGCCA TGG^CCA BbeI 760 GGCGCC GGCGC^C BcgI 1164 CGACAAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 1188 CCTAGG C^CTAGG BsaAI 3731 TACGTG [CT]AC^GT[GA] BsaBI 2904 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 3819 GAGACG CGTCTC(1/5) BsmI 1756 GAATGC GAATGC(1/-1) Bsp1407I 1885 TGTACA T^GTACA BspDI 1276 ATCGAT AT^CGAT BspLU11I 1478 ACATGT A^CATGT BsrGI 1885 TGTACA T^GTACA BstXI 1322 CCAAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 1276 ATCGAT AT^CGAT DrdI 477 GACAATTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 3722 CGGCCG C^GGCCG Eam1105I 3719 GACCGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 3722 CGGCCG C^GGCCG Eco47III 1097 AGCGCT AGC^GCT Eco52I 3722 CGGCCG C^GGCCG EcoRI 4177 GAATTC G^AATTC EcoT22I 3061 ATGCAT ATGCA^T FspI 2906 TGCGCA TGC^GCA KasI 760 GGCGCC G^GCGCC KpnI 3437 GGTACC GGTAC^C MamI 2904 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 479 CAATTG C^AATTG MluNI 1878 TGGCCA TGG^CCA MscI 1878 TGGCCA TGG^CCA MunI 479 CAATTG C^AATTG NarI 760 GGCGCC GG^CGCC NheI 3385 GCTAGC G^CTAGC NruI 10 TCGCGA TCG^CGA NsiI 3061 ATGCAT ATGCA^T PaeR7I 2948 CTCGAG C^TCGAG PflMI 394 CCAAAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 744 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 4001 CTGCAG CTGCA^G SalI 1183 GTCGAC G^TCGAC SexAI 3508 ACCTGGT A^CC[AT]GGT SfoI 760 GGCGCC GGC^GCC SmaI 756 CCCGGG CCC^GGG SspBI 1885 TGTACA T^GTACA Van91I 394 CCAAAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 2948 CTCGAG C^TCGAG XmaCI 756 CCCGGG C^CCGGG XmaI 756 CCCGGG C^CCGGG XmnI 3903 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccI 751 GTCTAC GT^[AC][GT]AC 1183 GTCGAC AccIII 1080 TCCGGA T^CCGGA 3911 TCCGGA AcyI 760 GGCGCC G[GA]^CG[CT]C 3821 GACGCC AflII 706 CTTAAG C^TTAAG 2686 CTTAAG AlwNI 735 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG 2394 CAGAGACTG AocI 922 CCTGAGG CC^T[ACGT]AGG 3894 CCTGAGG AvaI 756 CCCGGG C^[CT]CG[GA]G 2948 CTCGAG BfrI 706 CTTAAG C^TTAAG 2686 CTTAAG BglII 564 AGATCT A^GATCT 2444 AGATCT BsaHI 760 GGCGCC G[GA]^CG[CT]C 3821 GACGCC BseAI 1080 TCCGGA T^CCGGA 3911 TCCGGA BsiEI 36 CGGTCG CG[GA][CT]^CG 3722 CGGCCG BsoBI 756 CCCGGG C^[CT]CG[GA]G 2948 CTCGAG BspEI 1080 TCCGGA T^CCGGA 3911 TCCGGA BspHI 3741 TCATGA T^CATGA 4035 TCATGA BsrFI 830 GCCGGT [GA]^CCGG[CT] 3720 ACCGGC Bsu36I 922 CCTGAGG CC^T[ACGT]AGG 3894 CCTGAGG Cfr10I 830 GCCGGT [GA]^CCGG[CT] 3720 ACCGGC DraI 726 TTTAAA TTT^AAA 3235 TTTAAA EaeI 1878 TGGCCA [CT]^GGCC[GA] 3722 CGGCCG Eco81I 922 CCTGAGG CC^T[ACGT]AGG 3894 CCTGAGG EcoNI 290 CCTTTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2165 CCTTTCTCAGG FauI 2254 CCCGC CCCGC(4/6) 4073 CCCGC HaeII 760 GGCGCC [GA]GCGC^[CT] 1097 AGCGCT Hin1I 760 GGCGCC G[GA]^CG[CT]C 3821 GACGCC HincII 340 GTTGAC GT[CT]^[GA]AC 1183 GTCGAC HindII 340 GTTGAC GT[CT]^[GA]AC 1183 GTCGAC MroI 1080 TCCGGA T^CCGGA 3911 TCCGGA MslI 330 CATTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1819 CATGCGGATG PvuII 269 CAGCTG CAG^CTG 3535 CAGCTG RcaI 3741 TCATGA T^CATGA 4035 TCATGA SspI 3240 AATATT AAT^ATT 4225 AATATT StuI 856 AGGCCT AGG^CCT 2162 AGGCCT XcmI 68 CCAGGCTGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2336 CCAGATTCCCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccII AciI AcsI AfaI AluI ApoI AspHI AvaII BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstBI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI NspV PleI PpuMI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfuI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII