##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AgeI ApaI AscI
AseI AsnI AspI BamHI BbrPI
BclI BlpI Bpu1102I BsiWI BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstZ17I CelII CpoI
DraIII EcoO65I EcoRV FbaI FseI
HpaI KspI MluI NaeI NdeI
NgoMIV NotI PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SbfI ScaI
SfiI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 3437 GGTACC G^GTACC
AflIII 1478 ACATGT A^C[GA][CT]GT
AhdI 3719 GACCGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2503 GTGCAC G^TGCAC
AlwI 1011 GATCC GGATC(4/5)
Aor51HI 1097 AGCGCT AGC^GCT
ApaLI 2503 GTGCAC G^TGCAC
Asp700I 3903 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3437 GGTACC G^GTACC
AspEI 3719 GACCGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2906 TGCGCA TGC^GCA
AvrII 1188 CCTAGG C^CTAGG
BalI 1878 TGGCCA TGG^CCA
BbeI 760 GGCGCC GGCGC^C
BcgI 1164 CGACAAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1188 CCTAGG C^CTAGG
BsaAI 3731 TACGTG [CT]AC^GT[GA]
BsaBI 2904 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 3819 GAGACG CGTCTC(1/5)
BsmI 1756 GAATGC GAATGC(1/-1)
Bsp1407I 1885 TGTACA T^GTACA
BspDI 1276 ATCGAT AT^CGAT
BspLU11I 1478 ACATGT A^CATGT
BsrGI 1885 TGTACA T^GTACA
BstXI 1322 CCAAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1276 ATCGAT AT^CGAT
DrdI 477 GACAATTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3722 CGGCCG C^GGCCG
Eam1105I 3719 GACCGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3722 CGGCCG C^GGCCG
Eco47III 1097 AGCGCT AGC^GCT
Eco52I 3722 CGGCCG C^GGCCG
EcoRI 4177 GAATTC G^AATTC
EcoT22I 3061 ATGCAT ATGCA^T
FspI 2906 TGCGCA TGC^GCA
KasI 760 GGCGCC G^GCGCC
KpnI 3437 GGTACC GGTAC^C
MamI 2904 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 479 CAATTG C^AATTG
MluNI 1878 TGGCCA TGG^CCA
MscI 1878 TGGCCA TGG^CCA
MunI 479 CAATTG C^AATTG
NarI 760 GGCGCC GG^CGCC
NheI 3385 GCTAGC G^CTAGC
NruI 10 TCGCGA TCG^CGA
NsiI 3061 ATGCAT ATGCA^T
PaeR7I 2948 CTCGAG C^TCGAG
PflMI 394 CCAAAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 744 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 4001 CTGCAG CTGCA^G
SalI 1183 GTCGAC G^TCGAC
SexAI 3508 ACCTGGT A^CC[AT]GGT
SfoI 760 GGCGCC GGC^GCC
SmaI 756 CCCGGG CCC^GGG
SspBI 1885 TGTACA T^GTACA
Van91I 394 CCAAAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2948 CTCGAG C^TCGAG
XmaCI 756 CCCGGG C^CCGGG
XmaI 756 CCCGGG C^CCGGG
XmnI 3903 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 751 GTCTAC GT^[AC][GT]AC
1183 GTCGAC
AccIII 1080 TCCGGA T^CCGGA
3911 TCCGGA
AcyI 760 GGCGCC G[GA]^CG[CT]C
3821 GACGCC
AflII 706 CTTAAG C^TTAAG
2686 CTTAAG
AlwNI 735 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
2394 CAGAGACTG
AocI 922 CCTGAGG CC^T[ACGT]AGG
3894 CCTGAGG
AvaI 756 CCCGGG C^[CT]CG[GA]G
2948 CTCGAG
BfrI 706 CTTAAG C^TTAAG
2686 CTTAAG
BglII 564 AGATCT A^GATCT
2444 AGATCT
BsaHI 760 GGCGCC G[GA]^CG[CT]C
3821 GACGCC
BseAI 1080 TCCGGA T^CCGGA
3911 TCCGGA
BsiEI 36 CGGTCG CG[GA][CT]^CG
3722 CGGCCG
BsoBI 756 CCCGGG C^[CT]CG[GA]G
2948 CTCGAG
BspEI 1080 TCCGGA T^CCGGA
3911 TCCGGA
BspHI 3741 TCATGA T^CATGA
4035 TCATGA
BsrFI 830 GCCGGT [GA]^CCGG[CT]
3720 ACCGGC
Bsu36I 922 CCTGAGG CC^T[ACGT]AGG
3894 CCTGAGG
Cfr10I 830 GCCGGT [GA]^CCGG[CT]
3720 ACCGGC
DraI 726 TTTAAA TTT^AAA
3235 TTTAAA
EaeI 1878 TGGCCA [CT]^GGCC[GA]
3722 CGGCCG
Eco81I 922 CCTGAGG CC^T[ACGT]AGG
3894 CCTGAGG
EcoNI 290 CCTTTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2165 CCTTTCTCAGG
FauI 2254 CCCGC CCCGC(4/6)
4073 CCCGC
HaeII 760 GGCGCC [GA]GCGC^[CT]
1097 AGCGCT
Hin1I 760 GGCGCC G[GA]^CG[CT]C
3821 GACGCC
HincII 340 GTTGAC GT[CT]^[GA]AC
1183 GTCGAC
HindII 340 GTTGAC GT[CT]^[GA]AC
1183 GTCGAC
MroI 1080 TCCGGA T^CCGGA
3911 TCCGGA
MslI 330 CATTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1819 CATGCGGATG
PvuII 269 CAGCTG CAG^CTG
3535 CAGCTG
RcaI 3741 TCATGA T^CATGA
4035 TCATGA
SspI 3240 AATATT AAT^ATT
4225 AATATT
StuI 856 AGGCCT AGG^CCT
2162 AGGCCT
XcmI 68 CCAGGCTGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2336 CCAGATTCCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AspHI AvaII BanI BanII
BbsI BbvCI BbvI BciVI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstBI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI NspV PleI PpuMI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfuI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII