##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
AhdI AscI Asp700I Asp718I AspEI
AspI AvrII BbeI BbvCI BcgI
BciVI BlnI BsaWI BseAI BsiEI
BsiWI BsmI BspDI BspEI BsrBI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV FseI HpaI KasI KpnI
KspI MroI NaeI NarI NgoMIV
NotI NruI NspV PaeR7I PflFI
PinAI PpuMI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SwaI Tth111I XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 4535 GACGTC GACGT^C
AcyI 4535 GACGTC G[GA]^CG[CT]C
AflII 2755 CTTAAG C^TTAAG
Alw44I 2125 GTGCAC G^TGCAC
Aor51HI 1169 AGCGCT AGC^GCT
ApaLI 2125 GTGCAC G^TGCAC
AseI 2543 ATTAAT AT^TAAT
AsnI 2543 ATTAAT AT^TAAT
AvaI 77 CCCGAG C^[CT]CG[GA]G
AviII 1875 TGCGCA TGC^GCA
BamHI 2819 GGATCC G^GATCC
BclI 1852 TGATCA T^GATCA
BfrI 2755 CTTAAG C^TTAAG
BglI 2395 GCCTTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3592 AGATCT A^GATCT
BlpI 1775 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1775 GCTGAGC GC^T[ACGT]AGC
BsaHI 4535 GACGTC G[GA]^CG[CT]C
BseRI 281 CTCCTC GAGGAG(10/8)
BsmBI 2053 CGTCTC CGTCTC(1/5)
BsoBI 77 CCCGAG C^[CT]CG[GA]G
BspHI 2131 TCATGA T^CATGA
BspLU11I 2858 ACATGT A^CATGT
BsrFI 3112 ACCGGC [GA]^CCGG[CT]
BstAPI 4265 GCAGGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1232 GGTCACC G^GT[ACGT]ACC
BstPI 1232 GGTCACC G^GT[ACGT]ACC
CelII 1775 GCTGAGC GC^T[ACGT]AGC
Cfr10I 3112 ACCGGC [GA]^CCGG[CT]
Eco47III 1169 AGCGCT AGC^GCT
EcoNI 614 CCTGGCGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1232 GGTCACC G^GT[ACGT]ACC
FbaI 1852 TGATCA T^GATCA
FspI 1875 TGCGCA TGC^GCA
HaeII 1169 AGCGCT [GA]GCGC^[CT]
Hin1I 4535 GACGTC G[GA]^CG[CT]C
HindIII 3393 AAGCTT A^AGCTT
MluI 2668 ACGCGT A^CGCGT
NheI 2081 GCTAGC G^CTAGC
PacI 1980 TTAATTAA TTAAT^TAA
PmeI 2986 GTTTAAAC GTTT^AAAC
PshBI 2543 ATTAAT AT^TAAT
RcaI 2131 TCATGA T^CATGA
SacI 3264 GAGCTC GAGCT^C
SapI 3173 GAAGAGC GCTCTTC(1/4)
ScaI 1741 AGTACT AGT^ACT
SfiI 2394 GGCCTTTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 519 ACTAGT A^CTAGT
SspI 4526 AATATT AAT^ATT
StuI 2163 AGGCCT AGG^CCT
TaqI 557 TCGA T^CGA
TthHB8I 557 TCGA T^CGA
XbaI 3300 TCTAGA T^CTAGA
XcmI 2380 CCATGGATAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 1897 CGCG CG^CG
2669 CGCG
BbrPI 402 CACGTG CAC^GTG
2122 CACGTG
BbsI 628 GAAGAC GAAGAC(2/6)
638 GAAGAC
BcnI 1891 CCGGG CC^[GC]GG
4247 CCCGG
BpuAI 628 GAAGAC GAAGAC(2/6)
638 GAAGAC
BsaBI 2542 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3587 GATGTAGATC
BsaI 390 GAGACC GGTCTC(1/5)
1670 GAGACC
BspMI 2233 GCAGGT ACCTGC(4/8)
2782 GCAGGT
BsrDI 3036 CATTGC GCAATG(2/0)
3413 GCAATG
BstUI 1897 CGCG CG^CG
2669 CGCG
DraII 27 AGGGCCC [GA]G^G[ACGT]CC[CT]
73 GGGGCCC
EcoO109I 27 AGGGCCC [GA]G^G[ACGT]CC[CT]
73 GGGGCCC
EcoT22I 1287 ATGCAT ATGCA^T
2488 ATGCAT
HgaI 1895 GACGC GACGC(5/10)
3304 GACGC
HincII 842 GTTGAC GT[CT]^[GA]AC
3399 GTTGAC
HindII 842 GTTGAC GT[CT]^[GA]AC
3399 GTTGAC
MamI 2542 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3587 GATGTAGATC
MfeI 3902 CAATTG C^AATTG
4597 CAATTG
MunI 3902 CAATTG C^AATTG
4597 CAATTG
MvnI 1897 CGCG CG^CG
2669 CGCG
NciI 1891 CCGGG CC^[GC]GG
4247 CCCGG
NcoI 2380 CCATGG C^CATGG
3144 CCATGG
NdeI 2999 CATATG CA^TATG
4413 CATATG
NsiI 1287 ATGCAT ATGCA^T
2488 ATGCAT
PmaCI 402 CACGTG CAC^GTG
2122 CACGTG
PmlI 402 CACGTG CAC^GTG
2122 CACGTG
PstI 954 CTGCAG CTGCA^G
1137 CTGCAG
PvuII 270 CAGCTG CAG^CTG
2623 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaII BalI BanI BanII
BbvI BfaI BmyI BpmI BsaAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
Eco81I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XhoII