################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## Acc65I AccI AccIII AclI AgeI AhdI AscI Asp700I Asp718I AspEI AspI AvrII BbeI BbvCI BcgI BciVI BlnI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI BsrBI BssHII BssSI Bst1107I BstBI BstXI BstZ17I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoRI EcoRV FseI HpaI KasI KpnI KspI MroI NaeI NarI NgoMIV NotI NruI NspV PaeR7I PflFI PinAI PpuMI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 4535 GACGTC GACGT^C AcyI 4535 GACGTC G[GA]^CG[CT]C AflII 2755 CTTAAG C^TTAAG Alw44I 2125 GTGCAC G^TGCAC Aor51HI 1169 AGCGCT AGC^GCT ApaLI 2125 GTGCAC G^TGCAC AseI 2543 ATTAAT AT^TAAT AsnI 2543 ATTAAT AT^TAAT AvaI 77 CCCGAG C^[CT]CG[GA]G AviII 1875 TGCGCA TGC^GCA BamHI 2819 GGATCC G^GATCC BclI 1852 TGATCA T^GATCA BfrI 2755 CTTAAG C^TTAAG BglI 2395 GCCTTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3592 AGATCT A^GATCT BlpI 1775 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1775 GCTGAGC GC^T[ACGT]AGC BsaHI 4535 GACGTC G[GA]^CG[CT]C BseRI 281 CTCCTC GAGGAG(10/8) BsmBI 2053 CGTCTC CGTCTC(1/5) BsoBI 77 CCCGAG C^[CT]CG[GA]G BspHI 2131 TCATGA T^CATGA BspLU11I 2858 ACATGT A^CATGT BsrFI 3112 ACCGGC [GA]^CCGG[CT] BstAPI 4265 GCAGGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1232 GGTCACC G^GT[ACGT]ACC BstPI 1232 GGTCACC G^GT[ACGT]ACC CelII 1775 GCTGAGC GC^T[ACGT]AGC Cfr10I 3112 ACCGGC [GA]^CCGG[CT] Eco47III 1169 AGCGCT AGC^GCT EcoNI 614 CCTGGCGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1232 GGTCACC G^GT[ACGT]ACC FbaI 1852 TGATCA T^GATCA FspI 1875 TGCGCA TGC^GCA HaeII 1169 AGCGCT [GA]GCGC^[CT] Hin1I 4535 GACGTC G[GA]^CG[CT]C HindIII 3393 AAGCTT A^AGCTT MluI 2668 ACGCGT A^CGCGT NheI 2081 GCTAGC G^CTAGC PacI 1980 TTAATTAA TTAAT^TAA PmeI 2986 GTTTAAAC GTTT^AAAC PshBI 2543 ATTAAT AT^TAAT RcaI 2131 TCATGA T^CATGA SacI 3264 GAGCTC GAGCT^C SapI 3173 GAAGAGC GCTCTTC(1/4) ScaI 1741 AGTACT AGT^ACT SfiI 2394 GGCCTTTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 519 ACTAGT A^CTAGT SspI 4526 AATATT AAT^ATT StuI 2163 AGGCCT AGG^CCT TaqI 557 TCGA T^CGA TthHB8I 557 TCGA T^CGA XbaI 3300 TCTAGA T^CTAGA XcmI 2380 CCATGGATAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 1897 CGCG CG^CG 2669 CGCG BbrPI 402 CACGTG CAC^GTG 2122 CACGTG BbsI 628 GAAGAC GAAGAC(2/6) 638 GAAGAC BcnI 1891 CCGGG CC^[GC]GG 4247 CCCGG BpuAI 628 GAAGAC GAAGAC(2/6) 638 GAAGAC BsaBI 2542 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3587 GATGTAGATC BsaI 390 GAGACC GGTCTC(1/5) 1670 GAGACC BspMI 2233 GCAGGT ACCTGC(4/8) 2782 GCAGGT BsrDI 3036 CATTGC GCAATG(2/0) 3413 GCAATG BstUI 1897 CGCG CG^CG 2669 CGCG DraII 27 AGGGCCC [GA]G^G[ACGT]CC[CT] 73 GGGGCCC EcoO109I 27 AGGGCCC [GA]G^G[ACGT]CC[CT] 73 GGGGCCC EcoT22I 1287 ATGCAT ATGCA^T 2488 ATGCAT HgaI 1895 GACGC GACGC(5/10) 3304 GACGC HincII 842 GTTGAC GT[CT]^[GA]AC 3399 GTTGAC HindII 842 GTTGAC GT[CT]^[GA]AC 3399 GTTGAC MamI 2542 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3587 GATGTAGATC MfeI 3902 CAATTG C^AATTG 4597 CAATTG MunI 3902 CAATTG C^AATTG 4597 CAATTG MvnI 1897 CGCG CG^CG 2669 CGCG NciI 1891 CCGGG CC^[GC]GG 4247 CCCGG NcoI 2380 CCATGG C^CATGG 3144 CCATGG NdeI 2999 CATATG CA^TATG 4413 CATATG NsiI 1287 ATGCAT ATGCA^T 2488 ATGCAT PmaCI 402 CACGTG CAC^GTG 2122 CACGTG PmlI 402 CACGTG CAC^GTG 2122 CACGTG PstI 954 CTGCAG CTGCA^G 1137 CTGCAG PvuII 270 CAGCTG CAG^CTG 2623 CAGCTG ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaII BalI BanI BanII BbvI BfaI BmyI BpmI BsaAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EarI Eco81I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII