##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AccII AccIII AcsI AflIII AgeI
AhdI ApoI AscI AseI AsnI
AspEI AspI AvrII BbrPI BcgI
BclI BlnI BsaAI BseAI BsiWI
BspDI BspEI BspLU11I BssHII BssSI
BstBI BstUI ClaI CpoI DraIII
Eam1105I EcoRI EcoRV EcoT22I FbaI
FseI HpaI KspI MfeI MluI
MroI MunI MvnI NdeI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I Van91I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 3147 GACGTC GACGT^C
AflII 4293 CTTAAG C^TTAAG
Aor51HI 1879 AGCGCT AGC^GCT
Asp700I 2120 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2437 TGCGCA TGC^GCA
BamHI 1260 GGATCC G^GATCC
BbeI 85 GGCGCC GGCGC^C
BbsI 2124 GTCTTC GAAGAC(2/6)
BciVI 2449 GTATCC GTATCC(6/5)
BfrI 4293 CTTAAG C^TTAAG
BpuAI 2124 GTCTTC GAAGAC(2/6)
BsaBI 1214 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2863 TCCGGT [AT]^CCGG[AT]
BsiEI 27 CGGCCG CG[GA][CT]^CG
BsmBI 71 GAGACG CGTCTC(1/5)
BsmI 778 GAATGC GAATGC(1/-1)
Bsp1407I 3837 TGTACA T^GTACA
BsrFI 39 GCCGGC [GA]^CCGG[CT]
BsrGI 3837 TGTACA T^GTACA
Bst1107I 399 GTATAC GTA^TAC
BstZ17I 399 GTATAC GTA^TAC
Cfr10I 39 GCCGGC [GA]^CCGG[CT]
DrdI 1504 GACTCTCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 27 CGGCCG C^GGCCG
EclXI 27 CGGCCG C^GGCCG
Eco47III 1879 AGCGCT AGC^GCT
Eco52I 27 CGGCCG C^GGCCG
FspI 2437 TGCGCA TGC^GCA
KasI 85 GGCGCC G^GCGCC
MamI 1214 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 39 GCCGGC GCC^GGC
NarI 85 GGCGCC GG^CGCC
NgoMIV 39 GCCGGC G^CCGGC
NheI 728 GCTAGC G^CTAGC
NspI 3287 ACATGC [GA]CATG^[CT]
PaeR7I 2649 CTCGAG C^TCGAG
PshAI 572 GACTCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfiI 1806 GGCCCCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 85 GGCGCC GGC^GCC
SspBI 3837 TGTACA T^GTACA
XhoI 2649 CTCGAG C^TCGAG
XmnI 2120 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AclI 297 AACGTT AA^CGTT
3156 AACGTT
AcyI 85 GGCGCC G[GA]^CG[CT]C
3147 GACGTC
BbvCI 375 GCTGAGG CCTCAGC(-5/-2)
2097 GCTGAGG
BglII 245 AGATCT A^GATCT
1749 AGATCT
BsaHI 85 GGCGCC G[GA]^CG[CT]C
3147 GACGTC
BspHI 2617 TCATGA T^CATGA
4124 TCATGA
BsrBI 2045 CCGCTC CCGCTC(-3/-3)
2937 GAGCGG
BstEII 3053 GGTGACC G^GT[ACGT]ACC
4360 GGTTACC
BstPI 3053 GGTGACC G^GT[ACGT]ACC
4360 GGTTACC
BstXI 2243 CCAGGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3473 CCAGGGGCATGG
DraI 1247 TTTAAA TTT^AAA
2883 TTTAAA
EcoNI 156 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1565 CCTGAGTGAGG
EcoO65I 3053 GGTGACC G^GT[ACGT]ACC
4360 GGTTACC
HaeII 85 GGCGCC [GA]GCGC^[CT]
1879 AGCGCT
HgaI 2380 GACGC GACGC(5/10)
2394 GACGC
Hin1I 85 GGCGCC G[GA]^CG[CT]C
3147 GACGTC
HincII 1326 GTCAAC GT[CT]^[GA]AC
1968 GTCAAC
HindII 1326 GTCAAC GT[CT]^[GA]AC
1968 GTCAAC
HindIII 336 AAGCTT A^AGCTT
4397 AAGCTT
NcoI 3431 CCATGG C^CATGG
3704 CCATGG
Psp1406I 297 AACGTT AA^CGTT
3156 AACGTT
RcaI 2617 TCATGA T^CATGA
4124 TCATGA
StuI 1959 AGGCCT AGG^CCT
3068 AGGCCT
TaqI 565 TCGA T^CGA
2650 TCGA
Tsp509I 1842 AATT ^AATT
4375 AATT
TthHB8I 565 TCGA T^CGA
2650 TCGA
XbaI 3161 TCTAGA T^CTAGA
3723 TCTAGA
XcmI 3812 CCACTTAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4345 CCAGGGAACAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
Acc65I AccI AciI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI Asp718I AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I TspRI
XhoII