##################################
# NO CUT ENZYMES (   65 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AhdI        AocI        ApaI        AscI        
Asp718I     AspEI       AspI        AviII       BamHI       
BcgI        BfrI        BglI        BglII       BseAI       
BsiWI       BspDI       BspEI       BsrBI       Bst1107I    
BstAPI      BstZ17I     Bsu36I      ClaI        CpoI        
Eam1105I    Eco81I      EcoRI       EcoRV       FseI        
FspI        HpaI        KpnI        MfeI        MluI        
MroI        MunI        NaeI        NgoMIV      NheI        
NruI        PacI        PaeR7I      PflFI       PmeI        
PpuMI       Psp1406I    PvuI        RsrII       SalI        
SapI        SbfI        SfiI        SgrAI       SnaBI       
Sse8387I    SwaI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AgeI          468  ACCGGT               A^CCGGT
Aor51HI      3149  AGCGCT               AGC^GCT
AseI         3467  ATTAAT               AT^TAAT
AsnI         3467  ATTAAT               AT^TAAT
Asp700I      2624  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII        4883  CCTAGG               C^CTAGG
BbeI         3919  GGCGCC               GGCGC^C
BbrPI        2043  CACGTG               CAC^GTG
BciVI        1023  GGATAC               GTATCC(6/5)
BlnI         4883  CCTAGG               C^CTAGG
BsaBI        1736  GATGTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         468  ACCGGT               [AT]^CCGG[AT]
BsiEI          64  CGGCCG               CG[GA][CT]^CG
Bsp1407I     1675  TGTACA               T^GTACA
BsrGI        1675  TGTACA               T^GTACA
BstBI        2386  TTCGAA               TT^CGAA
BstEII       1955  GGTCACC              G^GT[ACGT]ACC
BstPI        1955  GGTCACC              G^GT[ACGT]ACC
BstXI        3110  CCACAAAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII         848  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DrdI          170  GACCGCGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           64  CGGCCG               C^GGCCG
EclXI          64  CGGCCG               C^GGCCG
Eco47III     3149  AGCGCT               AGC^GCT
Eco52I         64  CGGCCG               C^GGCCG
EcoNI        4434  CCTTTGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      848  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      1955  GGTCACC              G^GT[ACGT]ACC
EcoT22I      4719  ATGCAT               ATGCA^T
HindIII      1665  AAGCTT               A^AGCTT
KasI         3919  GGCGCC               G^GCGCC
MamI         1736  GATGTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI         3919  GGCGCC               GG^CGCC
NotI           63  GCGGCCGC             GC^GGCCGC
NsiI         4719  ATGCAT               ATGCA^T
NspV         2386  TTCGAA               TT^CGAA
PflMI        2199  CCACTCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         468  ACCGGT               A^CCGGT
PmaCI        2043  CACGTG               CAC^GTG
PmlI         2043  CACGTG               CAC^GTG
PshAI        2302  GACTTGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3467  ATTAAT               AT^TAAT
SexAI         611  ACCAGGT              A^CC[AT]GGT
SfoI         3919  GGCGCC               GGC^GCC
SfuI         2386  TTCGAA               TT^CGAA
SmlI         2971  CTTGAG               C^T[CT][GA]AG
SpeI         4177  ACTAGT               A^CTAGT
SspBI        1675  TGTACA               T^GTACA
SspI         3443  AATATT               AAT^ATT
Van91I       2199  CCACTCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         2624  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

BalI         2560  TGGCCA               TGG^CCA
             2874  TGGCCA              
BclI         2239  TGATCA               T^GATCA
             4669  TGATCA              
BlpI          263  GCTGAGC              GC^T[ACGT]AGC
             4805  GCTTAGC             
Bpu1102I      263  GCTGAGC              GC^T[ACGT]AGC
             4805  GCTTAGC             
BsmBI         676  CGTCTC               CGTCTC(1/5)
             3724  CGTCTC              
BspHI        1627  TCATGA               T^CATGA
             2943  TCATGA              
BssHII        186  GCGCGC               G^CGCGC
              300  GCGCGC              
CelII         263  GCTGAGC              GC^T[ACGT]AGC
             4805  GCTTAGC             
DraI         4616  TTTAAA               TTT^AAA
             4656  TTTAAA              
FbaI         2239  TGATCA               T^GATCA
             4669  TGATCA              
HincII       2342  GTTGAC               GT[CT]^[GA]AC
             3955  GTCAAC              
HindII       2342  GTTGAC               GT[CT]^[GA]AC
             3955  GTCAAC              
KspI           32  CCGCGG               CCGC^GG
               40  CCGCGG              
MluNI        2560  TGGCCA               TGG^CCA
             2874  TGGCCA              
MscI         2560  TGGCCA               TGG^CCA
             2874  TGGCCA              
NdeI         2370  CATATG               CA^TATG
             2796  CATATG              
PvuII        2607  CAGCTG               CAG^CTG
             4273  CAGCTG              
RcaI         1627  TCATGA               T^CATGA
             2943  TCATGA              
SacI            2  GAGCTC               GAGCT^C
             2176  GAGCTC              
SacII          32  CCGCGG               CCGC^GG
               40  CCGCGG              
SmaI          550  CCCGGG               CCC^GGG
             4332  CCCGGG              
SphI         2963  GCATGC               GCATG^C
             4564  GCATGC              
StuI           88  AGGCCT               AGG^CCT
             3216  AGGCCT              
XcmI          310  CCAGGGATTATCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2414  CCAGTGGTCTCTTGG     
XmaCI         550  CCCGGG               C^CCGGG
             4332  CCCGGG              
XmaI          550  CCCGGG               C^CCGGG
             4332  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        Alw44I      AlwI        AlwNI       
ApaLI       ApoI        AspHI       AvaI        AvaII       
BanI        BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspLU11I    
BspMI       BsrDI       BsrFI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraIII      DsaI        EaeI        EarI        
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        RsaI        
Sau3AI      Sau96I      ScaI        ScrFI       SfaNI       
SfcI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII