################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AhdI AocI ApaI AscI Asp718I AspEI AspI AviII BamHI BcgI BfrI BglI BglII BseAI BsiWI BspDI BspEI BsrBI Bst1107I BstAPI BstZ17I Bsu36I ClaI CpoI Eam1105I Eco81I EcoRI EcoRV FseI FspI HpaI KpnI MfeI MluI MroI MunI NaeI NgoMIV NheI NruI PacI PaeR7I PflFI PmeI PpuMI Psp1406I PvuI RsrII SalI SapI SbfI SfiI SgrAI SnaBI Sse8387I SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AgeI 468 ACCGGT A^CCGGT Aor51HI 3149 AGCGCT AGC^GCT AseI 3467 ATTAAT AT^TAAT AsnI 3467 ATTAAT AT^TAAT Asp700I 2624 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 4883 CCTAGG C^CTAGG BbeI 3919 GGCGCC GGCGC^C BbrPI 2043 CACGTG CAC^GTG BciVI 1023 GGATAC GTATCC(6/5) BlnI 4883 CCTAGG C^CTAGG BsaBI 1736 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 468 ACCGGT [AT]^CCGG[AT] BsiEI 64 CGGCCG CG[GA][CT]^CG Bsp1407I 1675 TGTACA T^GTACA BsrGI 1675 TGTACA T^GTACA BstBI 2386 TTCGAA TT^CGAA BstEII 1955 GGTCACC G^GT[ACGT]ACC BstPI 1955 GGTCACC G^GT[ACGT]ACC BstXI 3110 CCACAAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 848 AGGCCCT [GA]G^G[ACGT]CC[CT] DrdI 170 GACCGCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 64 CGGCCG C^GGCCG EclXI 64 CGGCCG C^GGCCG Eco47III 3149 AGCGCT AGC^GCT Eco52I 64 CGGCCG C^GGCCG EcoNI 4434 CCTTTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 848 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 1955 GGTCACC G^GT[ACGT]ACC EcoT22I 4719 ATGCAT ATGCA^T HindIII 1665 AAGCTT A^AGCTT KasI 3919 GGCGCC G^GCGCC MamI 1736 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 3919 GGCGCC GG^CGCC NotI 63 GCGGCCGC GC^GGCCGC NsiI 4719 ATGCAT ATGCA^T NspV 2386 TTCGAA TT^CGAA PflMI 2199 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 468 ACCGGT A^CCGGT PmaCI 2043 CACGTG CAC^GTG PmlI 2043 CACGTG CAC^GTG PshAI 2302 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3467 ATTAAT AT^TAAT SexAI 611 ACCAGGT A^CC[AT]GGT SfoI 3919 GGCGCC GGC^GCC SfuI 2386 TTCGAA TT^CGAA SmlI 2971 CTTGAG C^T[CT][GA]AG SpeI 4177 ACTAGT A^CTAGT SspBI 1675 TGTACA T^GTACA SspI 3443 AATATT AAT^ATT Van91I 2199 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 2624 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### BalI 2560 TGGCCA TGG^CCA 2874 TGGCCA BclI 2239 TGATCA T^GATCA 4669 TGATCA BlpI 263 GCTGAGC GC^T[ACGT]AGC 4805 GCTTAGC Bpu1102I 263 GCTGAGC GC^T[ACGT]AGC 4805 GCTTAGC BsmBI 676 CGTCTC CGTCTC(1/5) 3724 CGTCTC BspHI 1627 TCATGA T^CATGA 2943 TCATGA BssHII 186 GCGCGC G^CGCGC 300 GCGCGC CelII 263 GCTGAGC GC^T[ACGT]AGC 4805 GCTTAGC DraI 4616 TTTAAA TTT^AAA 4656 TTTAAA FbaI 2239 TGATCA T^GATCA 4669 TGATCA HincII 2342 GTTGAC GT[CT]^[GA]AC 3955 GTCAAC HindII 2342 GTTGAC GT[CT]^[GA]AC 3955 GTCAAC KspI 32 CCGCGG CCGC^GG 40 CCGCGG MluNI 2560 TGGCCA TGG^CCA 2874 TGGCCA MscI 2560 TGGCCA TGG^CCA 2874 TGGCCA NdeI 2370 CATATG CA^TATG 2796 CATATG PvuII 2607 CAGCTG CAG^CTG 4273 CAGCTG RcaI 1627 TCATGA T^CATGA 2943 TCATGA SacI 2 GAGCTC GAGCT^C 2176 GAGCTC SacII 32 CCGCGG CCGC^GG 40 CCGCGG SmaI 550 CCCGGG CCC^GGG 4332 CCCGGG SphI 2963 GCATGC GCATG^C 4564 GCATGC StuI 88 AGGCCT AGG^CCT 3216 AGGCCT XcmI 310 CCAGGGATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2414 CCAGTGGTCTCTTGG XmaCI 550 CCCGGG C^CCGGG 4332 CCCGGG XmaI 550 CCCGGG C^CCGGG 4332 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrDI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraIII DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII