##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI AocI ApaI AscI
Asp718I AspEI AspI AviII BamHI
BcgI BfrI BglI BglII BseAI
BsiWI BspDI BspEI BsrBI Bst1107I
BstAPI BstZ17I Bsu36I ClaI CpoI
Eam1105I Eco81I EcoRI EcoRV FseI
FspI HpaI KpnI MfeI MluI
MroI MunI NaeI NgoMIV NheI
NruI PacI PaeR7I PflFI PmeI
PpuMI Psp1406I PvuI RsrII SalI
SapI SbfI SfiI SgrAI SnaBI
Sse8387I SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AgeI 468 ACCGGT A^CCGGT
Aor51HI 3149 AGCGCT AGC^GCT
AseI 3467 ATTAAT AT^TAAT
AsnI 3467 ATTAAT AT^TAAT
Asp700I 2624 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 4883 CCTAGG C^CTAGG
BbeI 3919 GGCGCC GGCGC^C
BbrPI 2043 CACGTG CAC^GTG
BciVI 1023 GGATAC GTATCC(6/5)
BlnI 4883 CCTAGG C^CTAGG
BsaBI 1736 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 468 ACCGGT [AT]^CCGG[AT]
BsiEI 64 CGGCCG CG[GA][CT]^CG
Bsp1407I 1675 TGTACA T^GTACA
BsrGI 1675 TGTACA T^GTACA
BstBI 2386 TTCGAA TT^CGAA
BstEII 1955 GGTCACC G^GT[ACGT]ACC
BstPI 1955 GGTCACC G^GT[ACGT]ACC
BstXI 3110 CCACAAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 848 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 170 GACCGCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 64 CGGCCG C^GGCCG
EclXI 64 CGGCCG C^GGCCG
Eco47III 3149 AGCGCT AGC^GCT
Eco52I 64 CGGCCG C^GGCCG
EcoNI 4434 CCTTTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 848 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1955 GGTCACC G^GT[ACGT]ACC
EcoT22I 4719 ATGCAT ATGCA^T
HindIII 1665 AAGCTT A^AGCTT
KasI 3919 GGCGCC G^GCGCC
MamI 1736 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 3919 GGCGCC GG^CGCC
NotI 63 GCGGCCGC GC^GGCCGC
NsiI 4719 ATGCAT ATGCA^T
NspV 2386 TTCGAA TT^CGAA
PflMI 2199 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 468 ACCGGT A^CCGGT
PmaCI 2043 CACGTG CAC^GTG
PmlI 2043 CACGTG CAC^GTG
PshAI 2302 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3467 ATTAAT AT^TAAT
SexAI 611 ACCAGGT A^CC[AT]GGT
SfoI 3919 GGCGCC GGC^GCC
SfuI 2386 TTCGAA TT^CGAA
SmlI 2971 CTTGAG C^T[CT][GA]AG
SpeI 4177 ACTAGT A^CTAGT
SspBI 1675 TGTACA T^GTACA
SspI 3443 AATATT AAT^ATT
Van91I 2199 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2624 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
BalI 2560 TGGCCA TGG^CCA
2874 TGGCCA
BclI 2239 TGATCA T^GATCA
4669 TGATCA
BlpI 263 GCTGAGC GC^T[ACGT]AGC
4805 GCTTAGC
Bpu1102I 263 GCTGAGC GC^T[ACGT]AGC
4805 GCTTAGC
BsmBI 676 CGTCTC CGTCTC(1/5)
3724 CGTCTC
BspHI 1627 TCATGA T^CATGA
2943 TCATGA
BssHII 186 GCGCGC G^CGCGC
300 GCGCGC
CelII 263 GCTGAGC GC^T[ACGT]AGC
4805 GCTTAGC
DraI 4616 TTTAAA TTT^AAA
4656 TTTAAA
FbaI 2239 TGATCA T^GATCA
4669 TGATCA
HincII 2342 GTTGAC GT[CT]^[GA]AC
3955 GTCAAC
HindII 2342 GTTGAC GT[CT]^[GA]AC
3955 GTCAAC
KspI 32 CCGCGG CCGC^GG
40 CCGCGG
MluNI 2560 TGGCCA TGG^CCA
2874 TGGCCA
MscI 2560 TGGCCA TGG^CCA
2874 TGGCCA
NdeI 2370 CATATG CA^TATG
2796 CATATG
PvuII 2607 CAGCTG CAG^CTG
4273 CAGCTG
RcaI 1627 TCATGA T^CATGA
2943 TCATGA
SacI 2 GAGCTC GAGCT^C
2176 GAGCTC
SacII 32 CCGCGG CCGC^GG
40 CCGCGG
SmaI 550 CCCGGG CCC^GGG
4332 CCCGGG
SphI 2963 GCATGC GCATG^C
4564 GCATGC
StuI 88 AGGCCT AGG^CCT
3216 AGGCCT
XcmI 310 CCAGGGATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2414 CCAGTGGTCTCTTGG
XmaCI 550 CCCGGG C^CCGGG
4332 CCCGGG
XmaI 550 CCCGGG C^CCGGG
4332 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaLI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspLU11I
BspMI BsrDI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraIII DsaI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII