##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AcyI AgeI AscI
AseI AsnI AviII BbeI BbrPI
BciVI BsaAI BsaBI BsaHI BsmBI
BspDI BspHI BsrDI BssHII Bst1107I
BstAPI BstZ17I ClaI CpoI DraI
DrdI EcoRV FseI FspI Hin1I
HincII HindII HpaI KasI KspI
MamI MfeI MluI MunI NarI
NheI NotI NruI PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 4605 GGTACC G^GTACC
AccII 27 CGCG CG^CG
AccIII 2409 TCCGGA T^CCGGA
AflII 2384 CTTAAG C^TTAAG
AflIII 4173 ACATGT A^C[GA][CT]GT
AhdI 2591 GACAGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4860 AGCGCT AGC^GCT
ApaI 3697 GGGCCC GGGCC^C
Asp718I 4605 GGTACC G^GTACC
AspEI 2591 GACAGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3143 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2462 CCTAGG C^CTAGG
BclI 3298 TGATCA T^GATCA
BfrI 2384 CTTAAG C^TTAAG
BglI 2654 GCCTAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4843 AGATCT A^GATCT
BlnI 2462 CCTAGG C^CTAGG
BlpI 4856 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4856 GCTCAGC GC^T[ACGT]AGC
BseAI 2409 TCCGGA T^CCGGA
BsiEI 578 CGGCCG CG[GA][CT]^CG
BsiWI 2024 CGTACG C^GTACG
BsmI 488 GCATTC GAATGC(1/-1)
Bsp1407I 4168 TGTACA T^GTACA
BspEI 2409 TCCGGA T^CCGGA
BspLU11I 4173 ACATGT A^CATGT
BsrGI 4168 TGTACA T^GTACA
BssSI 1541 CTCGTG CACGAG(-5/-1)
BstBI 1725 TTCGAA TT^CGAA
BstUI 27 CGCG CG^CG
CelII 4856 GCTCAGC GC^T[ACGT]AGC
EagI 578 CGGCCG C^GGCCG
Eam1105I 2591 GACAGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 578 CGGCCG C^GGCCG
Eco47III 4860 AGCGCT AGC^GCT
Eco52I 578 CGGCCG C^GGCCG
EcoRI 338 GAATTC G^AATTC
EcoT22I 5184 ATGCAT ATGCA^T
FbaI 3298 TGATCA T^GATCA
HindIII 830 AAGCTT A^AGCTT
KpnI 4605 GGTACC GGTAC^C
MroI 2409 TCCGGA T^CCGGA
MvnI 27 CGCG CG^CG
NdeI 815 CATATG CA^TATG
NsiI 5184 ATGCAT ATGCA^T
NspV 1725 TTCGAA TT^CGAA
PaeR7I 1332 CTCGAG C^TCGAG
PflFI 3143 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 4653 GCTCTTC GCTCTTC(1/4)
SfuI 1725 TTCGAA TT^CGAA
SmaI 2837 CCCGGG CCC^GGG
SspBI 4168 TGTACA T^GTACA
Tth111I 3143 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2712 TCTAGA T^CTAGA
XhoI 1332 CTCGAG C^TCGAG
XmaCI 2837 CCCGGG C^CCGGG
XmaI 2837 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 1847 GTAGAC GT^[AC][GT]AC
3149 GTCTAC
Alw44I 4370 GTGCAC G^TGCAC
4922 GTGCAC
ApaLI 4370 GTGCAC G^TGCAC
4922 GTGCAC
Asp700I 1255 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2401 GAATTGGTTC
BsaWI 519 ACCGGA [AT]^CCGG[AT]
2409 TCCGGA
BsgI 2130 CTGCAC GTGCAG(16/14)
4937 CTGCAC
BsrBI 1712 GAGCGG CCGCTC(-3/-3)
2362 GAGCGG
EarI 4654 CTCTTC CTCTTC(1/4)
3739 GAAGAG
HgaI 2100 GACGC GACGC(5/10)
28 GCGTC
Ksp632I 4654 CTCTTC CTCTTC(1/4)
3739 GAAGAG
NaeI 576 GCCGGC GCC^GGC
594 GCCGGC
NgoMIV 576 GCCGGC G^CCGGC
594 GCCGGC
NspI 4173 ACATGT [GA]CATG^[CT]
5182 ACATGC
PshAI 2022 GACGTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3869 GACTGAGGTC
SacI 2605 GAGCTC GAGCT^C
3336 GAGCTC
ScaI 3117 AGTACT AGT^ACT
3492 AGTACT
SexAI 2310 ACCTGGT A^CC[AT]GGT
5053 ACCTGGT
TaqI 1333 TCGA T^CGA
1726 TCGA
TfiI 50 GAATC G^A[AT]TC
529 GAATC
TthHB8I 1333 TCGA T^CGA
1726 TCGA
XmnI 1255 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2401 GAATTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII