################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AscI AseI AsnI Asp700I Asp718I AviII BalI BfrI BlpI Bpu1102I BsaBI BsaWI BseAI BsiWI BspDI BspEI BspHI BssHII BstAPI BstEII BstPI CelII ClaI CpoI DraIII EcoO65I EcoRV EcoT22I FseI FspI KpnI KspI MamI MluI MluNI MroI MscI NotI NruI NsiI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SexAI SfiI SgrAI SnaBI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 3634 GTATAC GT^[AC][GT]AC AhdI 3530 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3700 GTGCAC G^TGCAC AocI 1600 CCTCAGG CC^T[ACGT]AGG Aor51HI 605 AGCGCT AGC^GCT ApaI 385 GGGCCC GGGCC^C ApaLI 3700 GTGCAC G^TGCAC AspEI 3530 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 2345 CCTAGG C^CTAGG BamHI 1054 GGATCC G^GATCC BbrPI 2168 CACGTG CAC^GTG BbsI 1493 GTCTTC GAAGAC(2/6) BciVI 2461 GGATAC GTATCC(6/5) BglII 2812 AGATCT A^GATCT BlnI 2345 CCTAGG C^CTAGG BpuAI 1493 GTCTTC GAAGAC(2/6) BspLU11I 3696 ACATGT A^CATGT BssSI 2973 CTCGTG CACGAG(-5/-1) Bst1107I 3634 GTATAC GTA^TAC BstBI 1925 TTCGAA TT^CGAA BstZ17I 3634 GTATAC GTA^TAC Bsu36I 1600 CCTCAGG CC^T[ACGT]AGG DrdI 2013 GACACAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 3530 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 605 AGCGCT AGC^GCT Eco81I 1600 CCTCAGG CC^T[ACGT]AGG EcoRI 1296 GAATTC G^AATTC HindIII 1909 AAGCTT A^AGCTT MfeI 1049 CAATTG C^AATTG MunI 1049 CAATTG C^AATTG NdeI 3152 CATATG CA^TATG NspV 1925 TTCGAA TT^CGAA PacI 3896 TTAATTAA TTAAT^TAA PmaCI 2168 CACGTG CAC^GTG PmlI 2168 CACGTG CAC^GTG PpuMI 518 AGGACCT [GA]G^G[AT]CC[CT] SacI 3472 GAGCTC GAGCT^C SapI 1663 GCTCTTC GCTCTTC(1/4) SbfI 1828 CCTGCAGG CCTGCA^GG SfuI 1925 TTCGAA TT^CGAA SmaI 1358 CCCGGG CCC^GGG SmlI 793 CTCAAG C^T[CT][GA]AG SpeI 4120 ACTAGT A^CTAGT SphI 1251 GCATGC GCATG^C Sse8387I 1828 CCTGCAGG CCTGCA^GG StuI 375 AGGCCT AGG^CCT XbaI 3270 TCTAGA T^CTAGA XcmI 533 CCACCTCCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1358 CCCGGG C^CCGGG XmaI 1358 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflIII 2609 ACACGT A^C[GA][CT]GT 3696 ACATGT BbvCI 2875 CCTCAGC CCTCAGC(-5/-2) 342 GCTGAGG BcgI 1320 GCAGTCGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1410 GCAGCAGCCTCG BclI 774 TGATCA T^GATCA 1200 TGATCA BglI 582 GCCGCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2694 GCCAGAAGGGC BsaAI 2168 CACGTG [CT]AC^GT[GA] 2610 CACGTA BsiEI 245 CGGCCG CG[GA][CT]^CG 548 CGGCCG BsmBI 1652 CGTCTC CGTCTC(1/5) 2142 GAGACG BsmI 1592 GAATGC GAATGC(1/-1) 2986 GAATGC Bsp1407I 1914 TGTACA T^GTACA 2383 TGTACA BsrGI 1914 TGTACA T^GTACA 2383 TGTACA BstXI 694 CCATTAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1695 CCATGGCTCTGG EagI 245 CGGCCG C^GGCCG 548 CGGCCG EclXI 245 CGGCCG C^GGCCG 548 CGGCCG Eco52I 245 CGGCCG C^GGCCG 548 CGGCCG EcoNI 1618 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2740 CCTGGCTCAGG FbaI 774 TGATCA T^GATCA 1200 TGATCA HpaI 262 GTTAAC GTT^AAC 2410 GTTAAC NcoI 1695 CCATGG C^CATGG 2209 CCATGG NheI 160 GCTAGC G^CTAGC 3478 GCTAGC PflMI 1218 CCAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2267 CCAATATCTGG ScaI 2557 AGTACT AGT^ACT 3133 AGTACT SspBI 1914 TGTACA T^GTACA 2383 TGTACA Van91I 1218 CCAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2267 CCAATATCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AspI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PflFI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII