##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AscI AseI AsnI Asp700I
Asp718I AviII BalI BfrI BlpI
Bpu1102I BsaBI BsaWI BseAI BsiWI
BspDI BspEI BspHI BssHII BstAPI
BstEII BstPI CelII ClaI CpoI
DraIII EcoO65I EcoRV EcoT22I FseI
FspI KpnI KspI MamI MluI
MluNI MroI MscI NotI NruI
NsiI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SexAI SfiI SgrAI
SnaBI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 3634 GTATAC GT^[AC][GT]AC
AhdI 3530 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3700 GTGCAC G^TGCAC
AocI 1600 CCTCAGG CC^T[ACGT]AGG
Aor51HI 605 AGCGCT AGC^GCT
ApaI 385 GGGCCC GGGCC^C
ApaLI 3700 GTGCAC G^TGCAC
AspEI 3530 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2345 CCTAGG C^CTAGG
BamHI 1054 GGATCC G^GATCC
BbrPI 2168 CACGTG CAC^GTG
BbsI 1493 GTCTTC GAAGAC(2/6)
BciVI 2461 GGATAC GTATCC(6/5)
BglII 2812 AGATCT A^GATCT
BlnI 2345 CCTAGG C^CTAGG
BpuAI 1493 GTCTTC GAAGAC(2/6)
BspLU11I 3696 ACATGT A^CATGT
BssSI 2973 CTCGTG CACGAG(-5/-1)
Bst1107I 3634 GTATAC GTA^TAC
BstBI 1925 TTCGAA TT^CGAA
BstZ17I 3634 GTATAC GTA^TAC
Bsu36I 1600 CCTCAGG CC^T[ACGT]AGG
DrdI 2013 GACACAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3530 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 605 AGCGCT AGC^GCT
Eco81I 1600 CCTCAGG CC^T[ACGT]AGG
EcoRI 1296 GAATTC G^AATTC
HindIII 1909 AAGCTT A^AGCTT
MfeI 1049 CAATTG C^AATTG
MunI 1049 CAATTG C^AATTG
NdeI 3152 CATATG CA^TATG
NspV 1925 TTCGAA TT^CGAA
PacI 3896 TTAATTAA TTAAT^TAA
PmaCI 2168 CACGTG CAC^GTG
PmlI 2168 CACGTG CAC^GTG
PpuMI 518 AGGACCT [GA]G^G[AT]CC[CT]
SacI 3472 GAGCTC GAGCT^C
SapI 1663 GCTCTTC GCTCTTC(1/4)
SbfI 1828 CCTGCAGG CCTGCA^GG
SfuI 1925 TTCGAA TT^CGAA
SmaI 1358 CCCGGG CCC^GGG
SmlI 793 CTCAAG C^T[CT][GA]AG
SpeI 4120 ACTAGT A^CTAGT
SphI 1251 GCATGC GCATG^C
Sse8387I 1828 CCTGCAGG CCTGCA^GG
StuI 375 AGGCCT AGG^CCT
XbaI 3270 TCTAGA T^CTAGA
XcmI 533 CCACCTCCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1358 CCCGGG C^CCGGG
XmaI 1358 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 2609 ACACGT A^C[GA][CT]GT
3696 ACATGT
BbvCI 2875 CCTCAGC CCTCAGC(-5/-2)
342 GCTGAGG
BcgI 1320 GCAGTCGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1410 GCAGCAGCCTCG
BclI 774 TGATCA T^GATCA
1200 TGATCA
BglI 582 GCCGCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2694 GCCAGAAGGGC
BsaAI 2168 CACGTG [CT]AC^GT[GA]
2610 CACGTA
BsiEI 245 CGGCCG CG[GA][CT]^CG
548 CGGCCG
BsmBI 1652 CGTCTC CGTCTC(1/5)
2142 GAGACG
BsmI 1592 GAATGC GAATGC(1/-1)
2986 GAATGC
Bsp1407I 1914 TGTACA T^GTACA
2383 TGTACA
BsrGI 1914 TGTACA T^GTACA
2383 TGTACA
BstXI 694 CCATTAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1695 CCATGGCTCTGG
EagI 245 CGGCCG C^GGCCG
548 CGGCCG
EclXI 245 CGGCCG C^GGCCG
548 CGGCCG
Eco52I 245 CGGCCG C^GGCCG
548 CGGCCG
EcoNI 1618 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2740 CCTGGCTCAGG
FbaI 774 TGATCA T^GATCA
1200 TGATCA
HpaI 262 GTTAAC GTT^AAC
2410 GTTAAC
NcoI 1695 CCATGG C^CATGG
2209 CCATGG
NheI 160 GCTAGC G^CTAGC
3478 GCTAGC
PflMI 1218 CCAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2267 CCAATATCTGG
ScaI 2557 AGTACT AGT^ACT
3133 AGTACT
SspBI 1914 TGTACA T^GTACA
2383 TGTACA
Van91I 1218 CCAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2267 CCAATATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AspI AvaI AvaII BanI BanII
BbeI BbvI BcnI BfaI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII