##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Aor51HI ApaI AscI AseI AsnI
Asp718I AvrII BamHI BbeI BfrI
BlnI BlpI Bpu1102I BsaBI BseAI
BsiWI BspEI BspHI BsrBI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
CpoI DrdI Eco47III EcoO65I EcoRI
EcoT22I FseI HindIII HpaI KasI
KpnI MamI MluI MroI NarI
NdeI NruI NsiI NspV PacI
PflMI PinAI PshBI Psp1406I RcaI
RsrII SalI SbfI ScaI SfoI
SfuI SmaI SpeI SphI Sse8387I
SwaI Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 3728 GACGTC GACGT^C
AcyI 3728 GACGTC G[GA]^CG[CT]C
AhdI 795 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 633 GTGCAC G^TGCAC
ApaLI 633 GTGCAC G^TGCAC
AspEI 795 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 274 TGCGCA TGC^GCA
BanI 2857 GGCACC G^G[CT][GA]CC
BbvCI 680 GCTGAGG CCTCAGC(-5/-2)
BsaHI 3728 GACGTC G[GA]^CG[CT]C
BsaWI 2238 TCCGGT [AT]^CCGG[AT]
BsmBI 395 CGTCTC CGTCTC(1/5)
BsmI 3328 GAATGC GAATGC(1/-1)
BspLU11I 1602 ACATGT A^CATGT
BssHII 95 GCGCGC G^CGCGC
BssSI 270 CTCGTG CACGAG(-5/-1)
DraI 3041 TTTAAA TTT^AAA
DraIII 2627 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 151 CGGCCG C^GGCCG
Eam1105I 795 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 151 CGGCCG C^GGCCG
Eco52I 151 CGGCCG C^GGCCG
EcoNI 3733 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 274 TGCGCA TGC^GCA
Hin1I 3728 GACGTC G[GA]^CG[CT]C
NaeI 122 GCCGGC GCC^GGC
NgoMIV 122 GCCGGC G^CCGGC
NheI 200 GCTAGC G^CTAGC
NotI 150 GCGGCCGC GC^GGCCGC
PaeR7I 2303 CTCGAG C^TCGAG
PmeI 3040 GTTTAAAC GTTT^AAAC
PshAI 249 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 883 CGATCG CGAT^CG
SexAI 2232 ACCAGGT A^CC[AT]GGT
SfiI 37 GGCCGCGTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 121 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 1757 TACGTA TAC^GTA
SspI 2200 AATATT AAT^ATT
XbaI 2243 TCTAGA T^CTAGA
XcmI 1183 CCACAGTGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2303 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AlwI 2956 GATCC GGATC(4/5)
3546 GGATC
AocI 1477 CCTCAGG CC^T[ACGT]AGG
2032 CCTGAGG
Asp700I 1330 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1468 GAATTTGTTC
AspI 693 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2231 GACCAGGTC
BalI 1180 TGGCCA TGG^CCA
3802 TGGCCA
BbrPI 2969 CACGTG CAC^GTG
3272 CACGTG
BcgI 85 CGAGCCGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1124 CGACTCCCCTGC
BciVI 833 GGATAC GTATCC(6/5)
1535 GTATCC
BclI 499 TGATCA T^GATCA
1377 TGATCA
Bsp1407I 1965 TGTACA T^GTACA
3252 TGTACA
BspDI 1817 ATCGAT AT^CGAT
2735 ATCGAT
BsrFI 122 GCCGGC [GA]^CCGG[CT]
1697 ACCGGC
BsrGI 1965 TGTACA T^GTACA
3252 TGTACA
BstXI 319 CCATCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1238 CCACCACTCTGG
Bsu36I 1477 CCTCAGG CC^T[ACGT]AGG
2032 CCTGAGG
Cfr10I 122 GCCGGC [GA]^CCGG[CT]
1697 ACCGGC
ClaI 1817 ATCGAT AT^CGAT
2735 ATCGAT
Eco81I 1477 CCTCAGG CC^T[ACGT]AGG
2032 CCTGAGG
EcoRV 3094 GATATC GAT^ATC
3855 GATATC
FbaI 499 TGATCA T^GATCA
1377 TGATCA
HincII 864 GTTGAC GT[CT]^[GA]AC
1637 GTCAAC
HindII 864 GTTGAC GT[CT]^[GA]AC
1637 GTCAAC
KspI 3 CCGCGG CCGC^GG
148 CCGCGG
MfeI 2472 CAATTG C^AATTG
2925 CAATTG
MluNI 1180 TGGCCA TGG^CCA
3802 TGGCCA
MscI 1180 TGGCCA TGG^CCA
3802 TGGCCA
MunI 2472 CAATTG C^AATTG
2925 CAATTG
NcoI 244 CCATGG C^CATGG
1430 CCATGG
NspI 768 ACATGC [GA]CATG^[CT]
1602 ACATGT
PflFI 693 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2231 GACCAGGTC
PmaCI 2969 CACGTG CAC^GTG
3272 CACGTG
PmlI 2969 CACGTG CAC^GTG
3272 CACGTG
PvuII 2582 CAGCTG CAG^CTG
3755 CAGCTG
SacI 2782 GAGCTC GAGCT^C
2804 GAGCTC
SacII 3 CCGCGG CCGC^GG
148 CCGCGG
SspBI 1965 TGTACA T^GTACA
3252 TGTACA
StuI 358 AGGCCT AGG^CCT
969 AGGCCT
Tth111I 693 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2231 GACCAGGTC
XmnI 1330 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1468 GAATTTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwNI ApoI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII