##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AspEI AviII AvrII BamHI
BbeI BbvCI BcgI BclI BglI
BlnI BlpI BpmI Bpu1102I BsaHI
BsaWI BseAI BsmBI BspDI BspEI
BspHI BspMI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraIII DsaI Eam1105I
Eco81I EcoO65I EcoRV FbaI FseI
FspI Hin1I KasI KspI MroI
NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PinAI
PmeI PshAI Psp1406I PvuI RcaI
RsrII SacII SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 404 GGTACC G^GTACC
Aor51HI 15 AGCGCT AGC^GCT
Asp718I 404 GGTACC G^GTACC
AspI 3334 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2322 TGGCCA TGG^CCA
BanII 3351 GAGCTC G[GA]GC[CT]^C
BbrPI 410 CACGTG CAC^GTG
BglII 3145 AGATCT A^GATCT
BsaBI 1604 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1235 GAGGAG GAGGAG(10/8)
BsgI 2642 GTGCAG GTGCAG(16/14)
BsiWI 7 CGTACG C^GTACG
BspLU11I 2084 ACATGT A^CATGT
BsrBI 32 GAGCGG CCGCTC(-3/-3)
BsrFI 114 GCCGGT [GA]^CCGG[CT]
Cfr10I 114 GCCGGT [GA]^CCGG[CT]
DrdI 95 GACGGAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 112 CGGCCG C^GGCCG
EclXI 112 CGGCCG C^GGCCG
Eco47III 15 AGCGCT AGC^GCT
Eco52I 112 CGGCCG C^GGCCG
EcoNI 4089 CCTTCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 760 ATGCAT ATGCA^T
FauI 64 CCCGC CCCGC(4/6)
HaeII 15 AGCGCT [GA]GCGC^[CT]
HpaI 4071 GTTAAC GTT^AAC
KpnI 404 GGTACC GGTAC^C
MamI 1604 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 391 CAATTG C^AATTG
MluI 784 ACGCGT A^CGCGT
MluNI 2322 TGGCCA TGG^CCA
MscI 2322 TGGCCA TGG^CCA
MunI 391 CAATTG C^AATTG
NdeI 217 CATATG CA^TATG
NsiI 760 ATGCAT ATGCA^T
NspI 2084 ACATGT [GA]CATG^[CT]
PflFI 3334 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 410 CACGTG CAC^GTG
PmlI 410 CACGTG CAC^GTG
PpuMI 1398 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 4049 CAGCTG CAG^CTG
SacI 3351 GAGCTC GAGCT^C
SalI 24 GTCGAC G^TCGAC
SmaI 85 CCCGGG CCC^GGG
SwaI 3967 ATTTAAAT ATTT^AAAT
Tth111I 3334 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 85 CCCGGG C^CCGGG
XmaI 85 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccI 24 GTCGAC GT^[AC][GT]AC
2080 GTCTAC
AflII 2949 CTTAAG C^TTAAG
3870 CTTAAG
AlwNI 1048 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
1797 CAGGGTCTG
AseI 1761 ATTAAT AT^TAAT
2298 ATTAAT
AsnI 1761 ATTAAT AT^TAAT
2298 ATTAAT
Asp700I 2004 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2467 GAATATTTTC
AspHI 2545 GTGCTC G[AT]GC[AT]^C
3351 GAGCTC
AvaI 85 CCCGGG C^[CT]CG[GA]G
3549 CCCGAG
BanI 404 GGTACC G^G[CT][GA]CC
1775 GGCACC
BciVI 3613 GGATAC GTATCC(6/5)
4380 GGATAC
BcnI 85 CCCGG CC^[GC]GG
86 CCGGG
BfrI 2949 CTTAAG C^TTAAG
3870 CTTAAG
BsiHKAI 2545 GTGCTC G[AT]GC[AT]^C
3351 GAGCTC
BsoBI 85 CCCGGG C^[CT]CG[GA]G
3549 CCCGAG
BsrDI 1309 GCAATG GCAATG(2/0)
3433 GCAATG
BstAPI 657 GCAATAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4078 GCACTGTCTGC
BstBI 826 TTCGAA TT^CGAA
3939 TTCGAA
DraII 1009 AGGCCCC [GA]G^G[ACGT]CC[CT]
1398 GGGTCCT
EaeI 112 CGGCCG [CT]^GGCC[GA]
2322 TGGCCA
EcoO109I 1009 AGGCCCC [GA]G^G[ACGT]CC[CT]
1398 GGGTCCT
EcoRI 739 GAATTC G^AATTC
1379 GAATTC
EcoT14I 1402 CCTTGG C^C[AT][AT]GG
5085 CCTTGG
HapII 86 CCGG C^CGG
115 CCGG
HgaI 483 GCGTC GACGC(5/10)
4501 GCGTC
HindIII 2952 AAGCTT A^AGCTT
4665 AAGCTT
HpaII 86 CCGG C^CGG
115 CCGG
MspA1I 815 CCGCTG C[AC]G^C[GT]G
4049 CAGCTG
MspI 86 CCGG C^CGG
115 CCGG
NciI 85 CCCGG CC^[GC]GG
86 CCGGG
NspV 826 TTCGAA TT^CGAA
3939 TTCGAA
PflMI 1225 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2043 CCACCAACTGG
PshBI 1761 ATTAAT AT^TAAT
2298 ATTAAT
PstI 801 CTGCAG CTGCA^G
4488 CTGCAG
SfuI 826 TTCGAA TT^CGAA
3939 TTCGAA
StuI 2404 AGGCCT AGG^CCT
5082 AGGCCT
StyI 1402 CCTTGG C^C[AT][AT]GG
5085 CCTTGG
Van91I 1225 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2043 CCACCAACTGG
XbaI 2929 TCTAGA T^CTAGA
4448 TCTAGA
XcmI 2386 CCACTGAAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4609 CCAGAACTAGCGTGG
XmnI 2004 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2467 GAATATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AvaII BbsI
BbvI BfaI BmyI BpuAI BsaAI
BsaI BsaJI BsiEI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI EarI
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII