##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AgeI        
AhdI        Alw44I      AocI        ApaI        ApaLI       
AscI        AspEI       AviII       AvrII       BamHI       
BbeI        BbvCI       BcgI        BclI        BglI        
BlnI        BlpI        BpmI        Bpu1102I    BsaHI       
BsaWI       BseAI       BsmBI       BspDI       BspEI       
BspHI       BspMI       BssHII      BssSI       Bst1107I    
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraIII      DsaI        Eam1105I    
Eco81I      EcoO65I     EcoRV       FbaI        FseI        
FspI        Hin1I       KasI        KspI        MroI        
NaeI        NarI        NcoI        NgoMIV      NheI        
NotI        NruI        PacI        PaeR7I      PinAI       
PmeI        PshAI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I        404  GGTACC               G^GTACC
Aor51HI        15  AGCGCT               AGC^GCT
Asp718I       404  GGTACC               G^GTACC
AspI         3334  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         2322  TGGCCA               TGG^CCA
BanII        3351  GAGCTC               G[GA]GC[CT]^C
BbrPI         410  CACGTG               CAC^GTG
BglII        3145  AGATCT               A^GATCT
BsaBI        1604  GATTATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI        1235  GAGGAG               GAGGAG(10/8)
BsgI         2642  GTGCAG               GTGCAG(16/14)
BsiWI           7  CGTACG               C^GTACG
BspLU11I     2084  ACATGT               A^CATGT
BsrBI          32  GAGCGG               CCGCTC(-3/-3)
BsrFI         114  GCCGGT               [GA]^CCGG[CT]
Cfr10I        114  GCCGGT               [GA]^CCGG[CT]
DrdI           95  GACGGAGGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          112  CGGCCG               C^GGCCG
EclXI         112  CGGCCG               C^GGCCG
Eco47III       15  AGCGCT               AGC^GCT
Eco52I        112  CGGCCG               C^GGCCG
EcoNI        4089  CCTTCTAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       760  ATGCAT               ATGCA^T
FauI           64  CCCGC                CCCGC(4/6)
HaeII          15  AGCGCT               [GA]GCGC^[CT]
HpaI         4071  GTTAAC               GTT^AAC
KpnI          404  GGTACC               GGTAC^C
MamI         1604  GATTATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          391  CAATTG               C^AATTG
MluI          784  ACGCGT               A^CGCGT
MluNI        2322  TGGCCA               TGG^CCA
MscI         2322  TGGCCA               TGG^CCA
MunI          391  CAATTG               C^AATTG
NdeI          217  CATATG               CA^TATG
NsiI          760  ATGCAT               ATGCA^T
NspI         2084  ACATGT               [GA]CATG^[CT]
PflFI        3334  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         410  CACGTG               CAC^GTG
PmlI          410  CACGTG               CAC^GTG
PpuMI        1398  GGGTCCT              [GA]G^G[AT]CC[CT]
PvuII        4049  CAGCTG               CAG^CTG
SacI         3351  GAGCTC               GAGCT^C
SalI           24  GTCGAC               G^TCGAC
SmaI           85  CCCGGG               CCC^GGG
SwaI         3967  ATTTAAAT             ATTT^AAAT
Tth111I      3334  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI          85  CCCGGG               C^CCGGG
XmaI           85  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   40 enzymes) #
###################################

AccI           24  GTCGAC               GT^[AC][GT]AC
             2080  GTCTAC              
AflII        2949  CTTAAG               C^TTAAG
             3870  CTTAAG              
AlwNI        1048  CAGTTACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1797  CAGGGTCTG           
AseI         1761  ATTAAT               AT^TAAT
             2298  ATTAAT              
AsnI         1761  ATTAAT               AT^TAAT
             2298  ATTAAT              
Asp700I      2004  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2467  GAATATTTTC          
AspHI        2545  GTGCTC               G[AT]GC[AT]^C
             3351  GAGCTC              
AvaI           85  CCCGGG               C^[CT]CG[GA]G
             3549  CCCGAG              
BanI          404  GGTACC               G^G[CT][GA]CC
             1775  GGCACC              
BciVI        3613  GGATAC               GTATCC(6/5)
             4380  GGATAC              
BcnI           85  CCCGG                CC^[GC]GG
               86  CCGGG               
BfrI         2949  CTTAAG               C^TTAAG
             3870  CTTAAG              
BsiHKAI      2545  GTGCTC               G[AT]GC[AT]^C
             3351  GAGCTC              
BsoBI          85  CCCGGG               C^[CT]CG[GA]G
             3549  CCCGAG              
BsrDI        1309  GCAATG               GCAATG(2/0)
             3433  GCAATG              
BstAPI        657  GCAATAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4078  GCACTGTCTGC         
BstBI         826  TTCGAA               TT^CGAA
             3939  TTCGAA              
DraII        1009  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             1398  GGGTCCT             
EaeI          112  CGGCCG               [CT]^GGCC[GA]
             2322  TGGCCA              
EcoO109I     1009  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             1398  GGGTCCT             
EcoRI         739  GAATTC               G^AATTC
             1379  GAATTC              
EcoT14I      1402  CCTTGG               C^C[AT][AT]GG
             5085  CCTTGG              
HapII          86  CCGG                 C^CGG
              115  CCGG                
HgaI          483  GCGTC                GACGC(5/10)
             4501  GCGTC               
HindIII      2952  AAGCTT               A^AGCTT
             4665  AAGCTT              
HpaII          86  CCGG                 C^CGG
              115  CCGG                
MspA1I        815  CCGCTG               C[AC]G^C[GT]G
             4049  CAGCTG              
MspI           86  CCGG                 C^CGG
              115  CCGG                
NciI           85  CCCGG                CC^[GC]GG
               86  CCGGG               
NspV          826  TTCGAA               TT^CGAA
             3939  TTCGAA              
PflMI        1225  CCAATGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2043  CCACCAACTGG         
PshBI        1761  ATTAAT               AT^TAAT
             2298  ATTAAT              
PstI          801  CTGCAG               CTGCA^G
             4488  CTGCAG              
SfuI          826  TTCGAA               TT^CGAA
             3939  TTCGAA              
StuI         2404  AGGCCT               AGG^CCT
             5082  AGGCCT              
StyI         1402  CCTTGG               C^C[AT][AT]GG
             5085  CCTTGG              
Van91I       1225  CCAATGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2043  CCACCAACTGG         
XbaI         2929  TCTAGA               T^CTAGA
             4448  TCTAGA              
XcmI         2386  CCACTGAAGGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4609  CCAGAACTAGCGTGG     
XmnI         2004  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2467  GAATATTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        ApoI        AvaII       BbsI        
BbvI        BfaI        BmyI        BpuAI       BsaAI       
BsaI        BsaJI       BsiEI       BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    Bsp1407I    
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        EarI        
EcoRII      Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HincII      HindII      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MvaI        MvnI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspBI       SspI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII