################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII AccIII AclI AcyI AgeI AhdI Alw44I AocI ApaI ApaLI AscI AspEI AviII AvrII BamHI BbeI BbvCI BcgI BclI BglI BlnI BlpI BpmI Bpu1102I BsaHI BsaWI BseAI BsmBI BspDI BspEI BspHI BspMI BssHII BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraIII DsaI Eam1105I Eco81I EcoO65I EcoRV FbaI FseI FspI Hin1I KasI KspI MroI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SacII SapI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 404 GGTACC G^GTACC Aor51HI 15 AGCGCT AGC^GCT Asp718I 404 GGTACC G^GTACC AspI 3334 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2322 TGGCCA TGG^CCA BanII 3351 GAGCTC G[GA]GC[CT]^C BbrPI 410 CACGTG CAC^GTG BglII 3145 AGATCT A^GATCT BsaBI 1604 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 1235 GAGGAG GAGGAG(10/8) BsgI 2642 GTGCAG GTGCAG(16/14) BsiWI 7 CGTACG C^GTACG BspLU11I 2084 ACATGT A^CATGT BsrBI 32 GAGCGG CCGCTC(-3/-3) BsrFI 114 GCCGGT [GA]^CCGG[CT] Cfr10I 114 GCCGGT [GA]^CCGG[CT] DrdI 95 GACGGAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 112 CGGCCG C^GGCCG EclXI 112 CGGCCG C^GGCCG Eco47III 15 AGCGCT AGC^GCT Eco52I 112 CGGCCG C^GGCCG EcoNI 4089 CCTTCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 760 ATGCAT ATGCA^T FauI 64 CCCGC CCCGC(4/6) HaeII 15 AGCGCT [GA]GCGC^[CT] HpaI 4071 GTTAAC GTT^AAC KpnI 404 GGTACC GGTAC^C MamI 1604 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 391 CAATTG C^AATTG MluI 784 ACGCGT A^CGCGT MluNI 2322 TGGCCA TGG^CCA MscI 2322 TGGCCA TGG^CCA MunI 391 CAATTG C^AATTG NdeI 217 CATATG CA^TATG NsiI 760 ATGCAT ATGCA^T NspI 2084 ACATGT [GA]CATG^[CT] PflFI 3334 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 410 CACGTG CAC^GTG PmlI 410 CACGTG CAC^GTG PpuMI 1398 GGGTCCT [GA]G^G[AT]CC[CT] PvuII 4049 CAGCTG CAG^CTG SacI 3351 GAGCTC GAGCT^C SalI 24 GTCGAC G^TCGAC SmaI 85 CCCGGG CCC^GGG SwaI 3967 ATTTAAAT ATTT^AAAT Tth111I 3334 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 85 CCCGGG C^CCGGG XmaI 85 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AccI 24 GTCGAC GT^[AC][GT]AC 2080 GTCTAC AflII 2949 CTTAAG C^TTAAG 3870 CTTAAG AlwNI 1048 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG 1797 CAGGGTCTG AseI 1761 ATTAAT AT^TAAT 2298 ATTAAT AsnI 1761 ATTAAT AT^TAAT 2298 ATTAAT Asp700I 2004 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2467 GAATATTTTC AspHI 2545 GTGCTC G[AT]GC[AT]^C 3351 GAGCTC AvaI 85 CCCGGG C^[CT]CG[GA]G 3549 CCCGAG BanI 404 GGTACC G^G[CT][GA]CC 1775 GGCACC BciVI 3613 GGATAC GTATCC(6/5) 4380 GGATAC BcnI 85 CCCGG CC^[GC]GG 86 CCGGG BfrI 2949 CTTAAG C^TTAAG 3870 CTTAAG BsiHKAI 2545 GTGCTC G[AT]GC[AT]^C 3351 GAGCTC BsoBI 85 CCCGGG C^[CT]CG[GA]G 3549 CCCGAG BsrDI 1309 GCAATG GCAATG(2/0) 3433 GCAATG BstAPI 657 GCAATAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4078 GCACTGTCTGC BstBI 826 TTCGAA TT^CGAA 3939 TTCGAA DraII 1009 AGGCCCC [GA]G^G[ACGT]CC[CT] 1398 GGGTCCT EaeI 112 CGGCCG [CT]^GGCC[GA] 2322 TGGCCA EcoO109I 1009 AGGCCCC [GA]G^G[ACGT]CC[CT] 1398 GGGTCCT EcoRI 739 GAATTC G^AATTC 1379 GAATTC EcoT14I 1402 CCTTGG C^C[AT][AT]GG 5085 CCTTGG HapII 86 CCGG C^CGG 115 CCGG HgaI 483 GCGTC GACGC(5/10) 4501 GCGTC HindIII 2952 AAGCTT A^AGCTT 4665 AAGCTT HpaII 86 CCGG C^CGG 115 CCGG MspA1I 815 CCGCTG C[AC]G^C[GT]G 4049 CAGCTG MspI 86 CCGG C^CGG 115 CCGG NciI 85 CCCGG CC^[GC]GG 86 CCGGG NspV 826 TTCGAA TT^CGAA 3939 TTCGAA PflMI 1225 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2043 CCACCAACTGG PshBI 1761 ATTAAT AT^TAAT 2298 ATTAAT PstI 801 CTGCAG CTGCA^G 4488 CTGCAG SfuI 826 TTCGAA TT^CGAA 3939 TTCGAA StuI 2404 AGGCCT AGG^CCT 5082 AGGCCT StyI 1402 CCTTGG C^C[AT][AT]GG 5085 CCTTGG Van91I 1225 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2043 CCACCAACTGG XbaI 2929 TCTAGA T^CTAGA 4448 TCTAGA XcmI 2386 CCACTGAAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4609 CCAGAACTAGCGTGG XmnI 2004 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2467 GAATATTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI ApoI AvaII BbsI BbvI BfaI BmyI BpuAI BsaAI BsaI BsaJI BsiEI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII