##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AgeI AhdI Alw44I ApaLI AscI
Asp718I AspEI AspI AvrII BamHI
BanI BbeI BcnI BglI BlnI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BsrBI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoNI EcoRV EcoT22I
FauI FseI Hin1I HindIII KasI
KpnI KspI MluI MroI NarI
NciI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
Tth111I Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AclI 1069 AACGTT AA^CGTT
AflII 1101 CTTAAG C^TTAAG
AlwNI 1268 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 469 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2052 AGCGCT AGC^GCT
ApaI 3334 GGGCCC GGGCC^C
AvaI 2609 CCCGAG C^[CT]CG[GA]G
AviII 3071 TGCGCA TGC^GCA
BalI 964 TGGCCA TGG^CCA
BbrPI 574 CACGTG CAC^GTG
BbvCI 3430 CCTCAGC CCTCAGC(-5/-2)
BcgI 1898 CGACTCTAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1038 TGATCA T^GATCA
BfrI 1101 CTTAAG C^TTAAG
BlpI 2934 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2934 GCTGAGC GC^T[ACGT]AGC
BsaBI 327 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 2609 CCCGAG C^[CT]CG[GA]G
BspLU11I 3511 ACATGT A^CATGT
BsrDI 3321 CATTGC GCAATG(2/0)
BsrFI 1445 GCCGGC [GA]^CCGG[CT]
BstAPI 2493 GCAAGTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2681 CCATTATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 469 CCTGAGG CC^T[ACGT]AGG
CelII 2934 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1445 GCCGGC [GA]^CCGG[CT]
DraIII 1195 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2393 CCATGG C^C[GA][CT]GG
Eco47III 2052 AGCGCT AGC^GCT
Eco81I 469 CCTGAGG CC^T[ACGT]AGG
FbaI 1038 TGATCA T^GATCA
FspI 3071 TGCGCA TGC^GCA
HapII 1446 CCGG C^CGG
HgaI 1857 GACGC GACGC(5/10)
HpaII 1446 CCGG C^CGG
MamI 327 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1700 CAATTG C^AATTG
MluNI 964 TGGCCA TGG^CCA
MscI 964 TGGCCA TGG^CCA
MspI 1446 CCGG C^CGG
MunI 1700 CAATTG C^AATTG
NaeI 1445 GCCGGC GCC^GGC
NcoI 2393 CCATGG C^CATGG
NgoMIV 1445 GCCGGC G^CCGGC
NspI 3511 ACATGT [GA]CATG^[CT]
PmaCI 574 CACGTG CAC^GTG
PmlI 574 CACGTG CAC^GTG
PpuMI 1711 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1713 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1069 AACGTT AA^CGTT
ScaI 1936 AGTACT AGT^ACT
SwaI 3621 ATTTAAAT ATTT^AAAT
XbaI 3540 TCTAGA T^CTAGA
XcmI 1706 CCAGCAGGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 916 CGCG CG^CG
2461 CGCG
AseI 994 ATTAAT AT^TAAT
1393 ATTAAT
AsnI 994 ATTAAT AT^TAAT
1393 ATTAAT
Asp700I 3270 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3455 GAACTGTTTC
BciVI 1562 GTATCC GTATCC(6/5)
3715 GTATCC
BglII 299 AGATCT A^GATCT
2898 AGATCT
BsaAI 574 CACGTG [CT]AC^GT[GA]
1403 CACGTA
BsaI 2173 GAGACC GGTCTC(1/5)
499 GGTCTC
BsgI 661 GTGCAG GTGCAG(16/14)
1201 GTGCAG
BspHI 1577 TCATGA T^CATGA
3441 TCATGA
BstEII 161 GGTCACC G^GT[ACGT]ACC
2436 GGTTACC
BstPI 161 GGTCACC G^GT[ACGT]ACC
2436 GGTTACC
BstUI 916 CGCG CG^CG
2461 CGCG
DraI 630 TTTAAA TTT^AAA
3622 TTTAAA
EaeI 964 TGGCCA [CT]^GGCC[GA]
1447 CGGCCA
EcoO65I 161 GGTCACC G^GT[ACGT]ACC
2436 GGTTACC
EcoRI 3581 GAATTC G^AATTC
3820 GAATTC
HaeII 589 AGCGCC [GA]GCGC^[CT]
2052 AGCGCT
HpaI 1254 GTTAAC GTT^AAC
2619 GTTAAC
MspA1I 1679 CAGCTG C[AC]G^C[GT]G
2965 CAGCTG
MvnI 916 CGCG CG^CG
2461 CGCG
PshBI 994 ATTAAT AT^TAAT
1393 ATTAAT
PstI 295 CTGCAG CTGCA^G
2968 CTGCAG
PvuII 1679 CAGCTG CAG^CTG
2965 CAGCTG
RcaI 1577 TCATGA T^CATGA
3441 TCATGA
SacI 2855 GAGCTC GAGCT^C
2937 GAGCTC
TaqI 763 TCGA T^CGA
2385 TCGA
TthHB8I 763 TCGA T^CGA
2385 TCGA
XmnI 3270 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3455 GAACTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HhaI HinP1I HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII