################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AccI AccIII AcyI AgeI AhdI Alw44I ApaLI AscI Asp718I AspEI AspI AvrII BamHI BanI BbeI BcnI BglI BlnI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BsrBI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoRV EcoT22I FauI FseI Hin1I HindIII KasI KpnI KspI MluI MroI NarI NciI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AclI 1069 AACGTT AA^CGTT AflII 1101 CTTAAG C^TTAAG AlwNI 1268 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 469 CCTGAGG CC^T[ACGT]AGG Aor51HI 2052 AGCGCT AGC^GCT ApaI 3334 GGGCCC GGGCC^C AvaI 2609 CCCGAG C^[CT]CG[GA]G AviII 3071 TGCGCA TGC^GCA BalI 964 TGGCCA TGG^CCA BbrPI 574 CACGTG CAC^GTG BbvCI 3430 CCTCAGC CCTCAGC(-5/-2) BcgI 1898 CGACTCTAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1038 TGATCA T^GATCA BfrI 1101 CTTAAG C^TTAAG BlpI 2934 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2934 GCTGAGC GC^T[ACGT]AGC BsaBI 327 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsoBI 2609 CCCGAG C^[CT]CG[GA]G BspLU11I 3511 ACATGT A^CATGT BsrDI 3321 CATTGC GCAATG(2/0) BsrFI 1445 GCCGGC [GA]^CCGG[CT] BstAPI 2493 GCAAGTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2681 CCATTATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 469 CCTGAGG CC^T[ACGT]AGG CelII 2934 GCTGAGC GC^T[ACGT]AGC Cfr10I 1445 GCCGGC [GA]^CCGG[CT] DraIII 1195 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 2393 CCATGG C^C[GA][CT]GG Eco47III 2052 AGCGCT AGC^GCT Eco81I 469 CCTGAGG CC^T[ACGT]AGG FbaI 1038 TGATCA T^GATCA FspI 3071 TGCGCA TGC^GCA HapII 1446 CCGG C^CGG HgaI 1857 GACGC GACGC(5/10) HpaII 1446 CCGG C^CGG MamI 327 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1700 CAATTG C^AATTG MluNI 964 TGGCCA TGG^CCA MscI 964 TGGCCA TGG^CCA MspI 1446 CCGG C^CGG MunI 1700 CAATTG C^AATTG NaeI 1445 GCCGGC GCC^GGC NcoI 2393 CCATGG C^CATGG NgoMIV 1445 GCCGGC G^CCGGC NspI 3511 ACATGT [GA]CATG^[CT] PmaCI 574 CACGTG CAC^GTG PmlI 574 CACGTG CAC^GTG PpuMI 1711 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1713 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1069 AACGTT AA^CGTT ScaI 1936 AGTACT AGT^ACT SwaI 3621 ATTTAAAT ATTT^AAAT XbaI 3540 TCTAGA T^CTAGA XcmI 1706 CCAGCAGGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 916 CGCG CG^CG 2461 CGCG AseI 994 ATTAAT AT^TAAT 1393 ATTAAT AsnI 994 ATTAAT AT^TAAT 1393 ATTAAT Asp700I 3270 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3455 GAACTGTTTC BciVI 1562 GTATCC GTATCC(6/5) 3715 GTATCC BglII 299 AGATCT A^GATCT 2898 AGATCT BsaAI 574 CACGTG [CT]AC^GT[GA] 1403 CACGTA BsaI 2173 GAGACC GGTCTC(1/5) 499 GGTCTC BsgI 661 GTGCAG GTGCAG(16/14) 1201 GTGCAG BspHI 1577 TCATGA T^CATGA 3441 TCATGA BstEII 161 GGTCACC G^GT[ACGT]ACC 2436 GGTTACC BstPI 161 GGTCACC G^GT[ACGT]ACC 2436 GGTTACC BstUI 916 CGCG CG^CG 2461 CGCG DraI 630 TTTAAA TTT^AAA 3622 TTTAAA EaeI 964 TGGCCA [CT]^GGCC[GA] 1447 CGGCCA EcoO65I 161 GGTCACC G^GT[ACGT]ACC 2436 GGTTACC EcoRI 3581 GAATTC G^AATTC 3820 GAATTC HaeII 589 AGCGCC [GA]GCGC^[CT] 2052 AGCGCT HpaI 1254 GTTAAC GTT^AAC 2619 GTTAAC MspA1I 1679 CAGCTG C[AC]G^C[GT]G 2965 CAGCTG MvnI 916 CGCG CG^CG 2461 CGCG PshBI 994 ATTAAT AT^TAAT 1393 ATTAAT PstI 295 CTGCAG CTGCA^G 2968 CTGCAG PvuII 1679 CAGCTG CAG^CTG 2965 CAGCTG RcaI 1577 TCATGA T^CATGA 3441 TCATGA SacI 2855 GAGCTC GAGCT^C 2937 GAGCTC TaqI 763 TCGA T^CGA 2385 TCGA TthHB8I 763 TCGA T^CGA 2385 TCGA XmnI 3270 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3455 GAACTGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI AspHI AvaII BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII