##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AvaI AviII AvrII BalI
BbeI BbrPI BglI BlnI BlpI
Bpu1102I BsaHI BseAI BsiEI BsiWI
BsoBI BspEI BspLU11I BsrBI BssHII
Bst1107I BstAPI BstBI BstUI BstZ17I
CelII CpoI DrdI EagI EclXI
Eco47III Eco52I EcoNI EcoT22I FseI
FspI Hin1I HpaI KasI KspI
MluI MluNI MroI MscI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmlI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SpeI SphI Sse8387I
StuI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 2076 GGTACC G^GTACC
AccI 4255 GTAGAC GT^[AC][GT]AC
AhdI 561 GACACAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 3508 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2076 GGTACC G^GTACC
AspEI 561 GACACAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 554 GAGCAC G[AT]GC[AT]^C
AspI 128 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 415 GGATCC G^GATCC
BcgI 4392 CGAGAACGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 1793 TACGTA [CT]AC^GT[GA]
BsaWI 1356 TCCGGT [AT]^CCGG[AT]
BsiHKAI 554 GAGCAC G[AT]GC[AT]^C
BsmBI 1310 CGTCTC CGTCTC(1/5)
BsmI 1543 GAATGC GAATGC(1/-1)
BspDI 5104 ATCGAT AT^CGAT
BspHI 1446 TCATGA T^CATGA
BsrDI 1483 GCAATG GCAATG(2/0)
BsrFI 3184 GCCGGT [GA]^CCGG[CT]
BstXI 452 CCACCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 2797 GCGC GCG^C
Cfr10I 3184 GCCGGT [GA]^CCGG[CT]
ClaI 5104 ATCGAT AT^CGAT
EaeI 3182 TGGCCG [CT]^GGCC[GA]
Eam1105I 561 GACACAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4635 GAATTC G^AATTC
EcoRV 2368 GATATC GAT^ATC
FauI 2709 CCCGC CCCGC(4/6)
HaeII 2796 GGCGCT [GA]GCGC^[CT]
HgaI 567 GCGTC GACGC(5/10)
HhaI 2797 GCGC GCG^C
HinP1I 2797 GCGC G^CGC
HincII 5135 GTTGAC GT[CT]^[GA]AC
HindII 5135 GTTGAC GT[CT]^[GA]AC
HindIII 2748 AAGCTT A^AGCTT
KpnI 2076 GGTACC GGTAC^C
MfeI 1651 CAATTG C^AATTG
MspA1I 4872 CAGCTG C[AC]G^C[GT]G
MunI 1651 CAATTG C^AATTG
PflFI 128 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 3051 GTTTAAAC GTTT^AAAC
PstI 1090 CTGCAG CTGCA^G
PvuII 4872 CAGCTG CAG^CTG
RcaI 1446 TCATGA T^CATGA
ScaI 1291 AGTACT AGT^ACT
SnaBI 1793 TACGTA TAC^GTA
Tth111I 128 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 680 CCAGAGTAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3508 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflIII 1307 ACACGT A^C[GA][CT]GT
3784 ACGTGT
AocI 798 CCTGAGG CC^T[ACGT]AGG
3644 CCTGAGG
AseI 2983 ATTAAT AT^TAAT
4512 ATTAAT
AsnI 2983 ATTAAT AT^TAAT
4512 ATTAAT
BanII 1914 GGGCTC G[GA]GC[CT]^C
2706 GAGCCC
BbsI 1617 GAAGAC GAAGAC(2/6)
1179 GTCTTC
BbvCI 1073 CCTCAGC CCTCAGC(-5/-2)
3232 GCTGAGG
BciVI 3542 GGATAC GTATCC(6/5)
1205 GTATCC
BclI 3615 TGATCA T^GATCA
3748 TGATCA
BcnI 10 CCCGG CC^[GC]GG
76 CCCGG
BglII 1643 AGATCT A^GATCT
2054 AGATCT
BpuAI 1617 GAAGAC GAAGAC(2/6)
1179 GTCTTC
BsaI 2095 GAGACC GGTCTC(1/5)
2766 GGTCTC
BspMI 2829 ACCTGC ACCTGC(4/8)
1161 GCAGGT
BssSI 320 CTCGTG CACGAG(-5/-1)
4976 CTCGTG
BstEII 1170 GGTAACC G^GT[ACGT]ACC
4808 GGTTACC
BstPI 1170 GGTAACC G^GT[ACGT]ACC
4808 GGTTACC
Bsu36I 798 CCTGAGG CC^T[ACGT]AGG
3644 CCTGAGG
Cac8I 2708 GCCCGC GC[ACGT]^[ACGT]GC
2799 GCTGGC
Eco81I 798 CCTGAGG CC^T[ACGT]AGG
3644 CCTGAGG
EcoO65I 1170 GGTAACC G^GT[ACGT]ACC
4808 GGTTACC
FbaI 3615 TGATCA T^GATCA
3748 TGATCA
NciI 10 CCCGG CC^[GC]GG
76 CCCGG
NdeI 473 CATATG CA^TATG
4290 CATATG
NspI 1284 ACATGC [GA]CATG^[CT]
5131 GCATGT
PflMI 46 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
88 CCAGGCCCTGG
PpuMI 3735 AGGTCCC [GA]G^G[AT]CC[CT]
4435 GGGTCCT
PshBI 2983 ATTAAT AT^TAAT
4512 ATTAAT
SapI 2703 GAAGAGC GCTCTTC(1/4)
1397 GCTCTTC
SwaI 2626 ATTTAAAT ATTT^AAAT
5074 ATTTAAAT
Van91I 46 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
88 CCAGGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI ApoI AvaII BanI
BbvI BfaI BfrI BmyI BpmI
BsaBI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII