##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AclI AflII AscI AseI
AsnI Asp718I AspI AvrII BamHI
BbeI BciVI BclI BfrI BglII
BlnI BsaBI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspHI BsrGI BssHII
Bst1107I BstZ17I ClaI CpoI DraI
DrdI EagI EclXI Eco52I EcoRV
FbaI FseI HindIII HpaI KasI
KpnI MamI MfeI MluI MunI
NarI NotI NruI PacI PaeR7I
PflFI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SfoI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 70 GACGTC GACGT^C
AccI 2165 GTCGAC GT^[AC][GT]AC
AccII 11 CGCG CG^CG
AflIII 3339 ACATGT A^C[GA][CT]GT
AgeI 32 ACCGGT A^CCGGT
AhdI 110 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3137 GTGCAC G^TGCAC
AocI 3596 CCTGAGG CC^T[ACGT]AGG
ApaLI 3137 GTGCAC G^TGCAC
Asp700I 3049 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 110 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2864 CCCGAG C^[CT]CG[GA]G
BbrPI 3359 CACGTG CAC^GTG
BsmI 2549 GCATTC GAATGC(1/-1)
BsoBI 2864 CCCGAG C^[CT]CG[GA]G
BspLU11I 3339 ACATGT A^CATGT
BsrBI 1181 GAGCGG CCGCTC(-3/-3)
BssSI 3877 CACGAG CACGAG(-5/-1)
BstBI 726 TTCGAA TT^CGAA
BstEII 1820 GGTCACC G^GT[ACGT]ACC
BstPI 1820 GGTCACC G^GT[ACGT]ACC
BstUI 11 CGCG CG^CG
Bsu36I 3596 CCTGAGG CC^T[ACGT]AGG
Eam1105I 110 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3596 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1820 GGTCACC G^GT[ACGT]ACC
EcoRI 1013 GAATTC G^AATTC
EcoT22I 2144 ATGCAT ATGCA^T
KspI 10 CCGCGG CCGC^GG
MvnI 11 CGCG CG^CG
NaeI 1552 GCCGGC GCC^GGC
NgoMIV 1552 GCCGGC G^CCGGC
NheI 3729 GCTAGC G^CTAGC
NsiI 2144 ATGCAT ATGCA^T
NspV 726 TTCGAA TT^CGAA
PinAI 32 ACCGGT A^CCGGT
PmaCI 3359 CACGTG CAC^GTG
PmlI 3359 CACGTG CAC^GTG
SacI 2348 GAGCTC GAGCT^C
SacII 10 CCGCGG CCGC^GG
SalI 2165 GTCGAC G^TCGAC
SfuI 726 TTCGAA TT^CGAA
XmnI 3049 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 2946 TCCGGA T^CCGGA
3393 TCCGGA
AcsI 1013 GAATTC [GA]^AATT[CT]
4235 AAATTT
AcyI 70 GACGTC G[GA]^CG[CT]C
1415 GGCGTC
Aor51HI 2850 AGCGCT AGC^GCT
3555 AGCGCT
ApoI 1013 GAATTC [GA]^AATT[CT]
4235 AAATTT
BbsI 355 GAAGAC GAAGAC(2/6)
2608 GAAGAC
BcgI 2113 CGAGCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
606 GCACTGTTGTCG
BpuAI 355 GAAGAC GAAGAC(2/6)
2608 GAAGAC
BsaAI 911 CACGTA [CT]AC^GT[GA]
3359 CACGTG
BsaHI 70 GACGTC G[GA]^CG[CT]C
1415 GGCGTC
BseAI 2946 TCCGGA T^CCGGA
3393 TCCGGA
BspEI 2946 TCCGGA T^CCGGA
3393 TCCGGA
BstAPI 2245 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2480 GCAGGTACTGC
BstYI 3207 AGATCC [GA]^GATC[CT]
3639 AGATCC
Eco47III 2850 AGCGCT AGC^GCT
3555 AGCGCT
Hin1I 70 GACGTC G[GA]^CG[CT]C
1415 GGCGTC
HincII 2165 GTCGAC GT[CT]^[GA]AC
3972 GTTGAC
HindII 2165 GTCGAC GT[CT]^[GA]AC
3972 GTTGAC
MflI 3207 AGATCC [GA]^GATC[CT]
3639 AGATCC
MroI 2946 TCCGGA T^CCGGA
3393 TCCGGA
MseI 1356 TTAA T^TAA
4239 TTAA
NcoI 807 CCATGG C^CATGG
4031 CCATGG
NdeI 1716 CATATG CA^TATG
2667 CATATG
ScaI 2340 AGTACT AGT^ACT
4227 AGTACT
SexAI 675 ACCTGGT A^CC[AT]GGT
2382 ACCAGGT
SfiI 865 GGCCTTTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3418 GGCCCGACTGGCC
SmlI 1489 CTCAAG C^T[CT][GA]AG
2524 CTCAAG
TfiI 2011 GAATC G^A[AT]TC
2943 GATTC
Tru9I 1356 TTAA T^TAA
4239 TTAA
XhoII 3207 AGATCC [GA]^GATC[CT]
3639 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaII AviII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstXI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI Sse8387I StuI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI