##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
AocI Aor51HI AscI Asp718I AspEI
AviII AvrII BamHI BbsI BcgI
BciVI BclI BlnI BpuAI BsaBI
BsaWI BseAI BsiWI BspDI BspEI
BspMI Bst1107I BstAPI BstBI BstZ17I
Bsu36I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRV EcoT22I FbaI FseI FspI
HindIII KpnI MamI MluI MroI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmeI Psp1406I PvuI RsrII
SalI SapI SbfI ScaI SexAI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I StuI SwaI Van91I XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 1843 GTCTAC GT^[AC][GT]AC
AflII 3932 CTTAAG C^TTAAG
AlwI 519 GGATC GGATC(4/5)
ApaI 3012 GGGCCC GGGCC^C
AseI 2620 ATTAAT AT^TAAT
AsnI 2620 ATTAAT AT^TAAT
AspI 2071 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1342 CACGTG CAC^GTG
BbvCI 270 GCTGAGG CCTCAGC(-5/-2)
BfrI 3932 CTTAAG C^TTAAG
BglII 1589 AGATCT A^GATCT
BsaAI 1342 CACGTG [CT]AC^GT[GA]
BsiEI 1331 CGACCG CG[GA][CT]^CG
BsmBI 76 GAGACG CGTCTC(1/5)
BsmI 3740 GAATGC GAATGC(1/-1)
BspLU11I 2021 ACATGT A^CATGT
BssHII 700 GCGCGC G^CGCGC
BstEII 1281 GGTCACC G^GT[ACGT]ACC
BstPI 1281 GGTCACC G^GT[ACGT]ACC
BstXI 2629 CCAGGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoNI 2277 CCTCTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1281 GGTCACC G^GT[ACGT]ACC
EcoRI 3377 GAATTC G^AATTC
HpaI 2192 GTTAAC GTT^AAC
MfeI 1968 CAATTG C^AATTG
MunI 1968 CAATTG C^AATTG
PflFI 2071 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1342 CACGTG CAC^GTG
PmlI 1342 CACGTG CAC^GTG
PpuMI 1793 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 3786 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2620 ATTAAT AT^TAAT
SfiI 909 GGCCGAAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tth111I 2071 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3388 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 1186 GACGTC GACGT^C
1213 GACGTC
Alw44I 1006 GTGCAC G^TGCAC
4028 GTGCAC
ApaLI 1006 GTGCAC G^TGCAC
4028 GTGCAC
BsgI 733 CTGCAC GTGCAG(16/14)
832 GTGCAG
BspHI 365 TCATGA T^CATGA
2085 TCATGA
BsrDI 4093 CATTGC GCAATG(2/0)
3552 GCAATG
BsrFI 160 GCCGGC [GA]^CCGG[CT]
1705 GCCGGC
BssSI 2116 CTCGTG CACGAG(-5/-1)
3421 CTCGTG
BstYI 519 GGATCT [GA]^GATC[CT]
1589 AGATCT
Cfr10I 160 GCCGGC [GA]^CCGG[CT]
1705 GCCGGC
DrdI 3570 GACCTTTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3944 GACATTTATGTC
EcoT14I 506 CCAAGG C^C[AT][AT]GG
3665 CCTTGG
HincII 628 GTCAAC GT[CT]^[GA]AC
2192 GTTAAC
HindII 628 GTCAAC GT[CT]^[GA]AC
2192 GTTAAC
KspI 295 CCGCGG CCGC^GG
1334 CCGCGG
MflI 519 GGATCT [GA]^GATC[CT]
1589 AGATCT
NaeI 160 GCCGGC GCC^GGC
1705 GCCGGC
NgoMIV 160 GCCGGC G^CCGGC
1705 GCCGGC
NspI 2021 ACATGT [GA]CATG^[CT]
3630 ACATGC
PstI 2902 CTGCAG CTGCA^G
3679 CTGCAG
RcaI 365 TCATGA T^CATGA
2085 TCATGA
SacI 745 GAGCTC GAGCT^C
2081 GAGCTC
SacII 295 CCGCGG CCGC^GG
1334 CCGCGG
SmlI 2824 CTTGAG C^T[CT][GA]AG
3932 CTTAAG
SspI 2288 AATATT AAT^ATT
3919 AATATT
StyI 506 CCAAGG C^C[AT][AT]GG
3665 CCTTGG
XhoII 519 GGATCT [GA]^GATC[CT]
1589 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwNI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbeI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BsrBI BsrGI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SspBI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmaCI XmaI XmnI