##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AccIII AclI AflIII AgeI
AhdI Alw44I ApaLI AscI Asp700I
Asp718I AspEI BbrPI BsaBI BsaI
BsaWI BseAI BsiWI BsmI BspEI
BspLU11I Bst1107I BstBI BstZ17I CpoI
DraI Eam1105I HindIII KpnI MamI
MfeI MluI MroI MunI NheI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SalI SbfI SfuI SnaBI
Sse8387I SwaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AatII 99 GACGTC GACGT^C
AccI 2091 GTAGAC GT^[AC][GT]AC
AflII 2318 CTTAAG C^TTAAG
AocI 4815 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3400 AGCGCT AGC^GCT
AseI 4892 ATTAAT AT^TAAT
AsnI 4892 ATTAAT AT^TAAT
AspI 1292 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 255 TGCGCA TGC^GCA
AvrII 3573 CCTAGG C^CTAGG
BalI 384 TGGCCA TGG^CCA
BbvCI 546 CCTCAGC CCTCAGC(-5/-2)
BcgI 1445 GCAGAGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4034 TGATCA T^GATCA
BfrI 2318 CTTAAG C^TTAAG
BlnI 3573 CCTAGG C^CTAGG
BsaAI 4357 CACGTA [CT]AC^GT[GA]
BsmBI 3442 GAGACG CGTCTC(1/5)
Bsp1407I 5015 TGTACA T^GTACA
BspDI 4224 ATCGAT AT^CGAT
BspHI 3497 TCATGA T^CATGA
BsrGI 5015 TGTACA T^GTACA
BssHII 1013 GCGCGC G^CGCGC
BssSI 1825 CACGAG CACGAG(-5/-1)
BstEII 4789 GGTTACC G^GT[ACGT]ACC
BstPI 4789 GGTTACC G^GT[ACGT]ACC
BstXI 4718 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4815 CCTCAGG CC^T[ACGT]AGG
ClaI 4224 ATCGAT AT^CGAT
DraIII 3676 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1292 GACTAAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3400 AGCGCT AGC^GCT
Eco81I 4815 CCTCAGG CC^T[ACGT]AGG
EcoO65I 4789 GGTTACC G^GT[ACGT]ACC
EcoRI 4021 GAATTC G^AATTC
EcoRV 2562 GATATC GAT^ATC
EcoT22I 4689 ATGCAT ATGCA^T
FbaI 4034 TGATCA T^GATCA
FspI 255 TGCGCA TGC^GCA
HincII 5036 GTTAAC GT[CT]^[GA]AC
HindII 5036 GTTAAC GT[CT]^[GA]AC
HpaI 5036 GTTAAC GTT^AAC
MluNI 384 TGGCCA TGG^CCA
MscI 384 TGGCCA TGG^CCA
NcoI 4553 CCATGG C^CATGG
NdeI 4246 CATATG CA^TATG
NsiI 4689 ATGCAT ATGCA^T
PflFI 1292 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4143 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 282 GACGCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4892 ATTAAT AT^TAAT
RcaI 3497 TCATGA T^CATGA
SfiI 1127 GGCCTCAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1092 CACCGGCG C[GA]^CCGG[CT]G
SmaI 692 CCCGGG CCC^GGG
SpeI 4122 ACTAGT A^CTAGT
SphI 2012 GCATGC GCATG^C
SspBI 5015 TGTACA T^GTACA
SspI 4889 AATATT AAT^ATT
Tth111I 1292 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4143 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 692 CCCGGG C^CCGGG
XmaI 692 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
ApaI 695 GGGCCC GGGCC^C
768 GGGCCC
BglI 930 GCCAGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1128 GCCTCAAAGGC
BglII 1710 AGATCT A^GATCT
5116 AGATCT
BlpI 3411 GCTGAGC GC^T[ACGT]AGC
4182 GCTCAGC
BpmI 705 CTGGAG CTGGAG(16/14)
4150 CTGGAG
Bpu1102I 3411 GCTGAGC GC^T[ACGT]AGC
4182 GCTCAGC
BspMI 2153 ACCTGC ACCTGC(4/8)
5307 ACCTGC
CelII 3411 GCTGAGC GC^T[ACGT]AGC
4182 GCTCAGC
EcoNI 4590 CCTTCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4713 CCTGCCCAAGG
FseI 37 GGCCGGCC GGCCGG^CC
321 GGCCGGCC
SacI 87 GAGCTC GAGCT^C
893 GAGCTC
SapI 620 GCTCTTC GCTCTTC(1/4)
3420 GCTCTTC
SexAI 2809 ACCTGGT A^CC[AT]GGT
3677 ACCTGGT
TfiI 535 GATTC G^A[AT]TC
2853 GAATC
XbaI 1734 TCTAGA T^CTAGA
3926 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BamHI BanI BanII
BbeI BbsI BbvI BciVI BcnI
BfaI BmyI BpuAI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NotI
NspI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SfoI SmlI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII