################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## Acc65I AccIII AclI AflIII AgeI AhdI Alw44I ApaLI AscI Asp700I Asp718I AspEI BbrPI BsaBI BsaI BsaWI BseAI BsiWI BsmI BspEI BspLU11I Bst1107I BstBI BstZ17I CpoI DraI Eam1105I HindIII KpnI MamI MfeI MluI MroI MunI NheI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SalI SbfI SfuI SnaBI Sse8387I SwaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AatII 99 GACGTC GACGT^C AccI 2091 GTAGAC GT^[AC][GT]AC AflII 2318 CTTAAG C^TTAAG AocI 4815 CCTCAGG CC^T[ACGT]AGG Aor51HI 3400 AGCGCT AGC^GCT AseI 4892 ATTAAT AT^TAAT AsnI 4892 ATTAAT AT^TAAT AspI 1292 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 255 TGCGCA TGC^GCA AvrII 3573 CCTAGG C^CTAGG BalI 384 TGGCCA TGG^CCA BbvCI 546 CCTCAGC CCTCAGC(-5/-2) BcgI 1445 GCAGAGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 4034 TGATCA T^GATCA BfrI 2318 CTTAAG C^TTAAG BlnI 3573 CCTAGG C^CTAGG BsaAI 4357 CACGTA [CT]AC^GT[GA] BsmBI 3442 GAGACG CGTCTC(1/5) Bsp1407I 5015 TGTACA T^GTACA BspDI 4224 ATCGAT AT^CGAT BspHI 3497 TCATGA T^CATGA BsrGI 5015 TGTACA T^GTACA BssHII 1013 GCGCGC G^CGCGC BssSI 1825 CACGAG CACGAG(-5/-1) BstEII 4789 GGTTACC G^GT[ACGT]ACC BstPI 4789 GGTTACC G^GT[ACGT]ACC BstXI 4718 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 4815 CCTCAGG CC^T[ACGT]AGG ClaI 4224 ATCGAT AT^CGAT DraIII 3676 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1292 GACTAAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3400 AGCGCT AGC^GCT Eco81I 4815 CCTCAGG CC^T[ACGT]AGG EcoO65I 4789 GGTTACC G^GT[ACGT]ACC EcoRI 4021 GAATTC G^AATTC EcoRV 2562 GATATC GAT^ATC EcoT22I 4689 ATGCAT ATGCA^T FbaI 4034 TGATCA T^GATCA FspI 255 TGCGCA TGC^GCA HincII 5036 GTTAAC GT[CT]^[GA]AC HindII 5036 GTTAAC GT[CT]^[GA]AC HpaI 5036 GTTAAC GTT^AAC MluNI 384 TGGCCA TGG^CCA MscI 384 TGGCCA TGG^CCA NcoI 4553 CCATGG C^CATGG NdeI 4246 CATATG CA^TATG NsiI 4689 ATGCAT ATGCA^T PflFI 1292 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 4143 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 282 GACGCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4892 ATTAAT AT^TAAT RcaI 3497 TCATGA T^CATGA SfiI 1127 GGCCTCAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 1092 CACCGGCG C[GA]^CCGG[CT]G SmaI 692 CCCGGG CCC^GGG SpeI 4122 ACTAGT A^CTAGT SphI 2012 GCATGC GCATG^C SspBI 5015 TGTACA T^GTACA SspI 4889 AATATT AAT^ATT Tth111I 1292 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 4143 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 692 CCCGGG C^CCGGG XmaI 692 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### ApaI 695 GGGCCC GGGCC^C 768 GGGCCC BglI 930 GCCAGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1128 GCCTCAAAGGC BglII 1710 AGATCT A^GATCT 5116 AGATCT BlpI 3411 GCTGAGC GC^T[ACGT]AGC 4182 GCTCAGC BpmI 705 CTGGAG CTGGAG(16/14) 4150 CTGGAG Bpu1102I 3411 GCTGAGC GC^T[ACGT]AGC 4182 GCTCAGC BspMI 2153 ACCTGC ACCTGC(4/8) 5307 ACCTGC CelII 3411 GCTGAGC GC^T[ACGT]AGC 4182 GCTCAGC EcoNI 4590 CCTTCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4713 CCTGCCCAAGG FseI 37 GGCCGGCC GGCCGG^CC 321 GGCCGGCC SacI 87 GAGCTC GAGCT^C 893 GAGCTC SapI 620 GCTCTTC GCTCTTC(1/4) 3420 GCTCTTC SexAI 2809 ACCTGGT A^CC[AT]GGT 3677 ACCTGGT TfiI 535 GATTC G^A[AT]TC 2853 GAATC XbaI 1734 TCTAGA T^CTAGA 3926 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BamHI BanI BanII BbeI BbsI BbvI BciVI BcnI BfaI BmyI BpuAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NotI NspI PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SfoI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII