################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII AccIII AflII Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AviII AvrII BamHI BcgI BfrI BglI BlnI BsaI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BsrBI BsrGI BssHII Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FseI FspI KspI MfeI MroI MunI NotI NruI NsiI PacI PaeR7I PmeI PshBI PvuI RsrII SacII SalI SbfI SfiI SmaI SnaBI Sse8387I SspBI SspI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 3311 GGTACC G^GTACC AccI 3508 GTAGAC GT^[AC][GT]AC AclI 4036 AACGTT AA^CGTT AgeI 1747 ACCGGT A^CCGGT AhdI 3291 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 3311 GGTACC G^GTACC AspEI 3291 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 4757 GGCGCC GGCGC^C BbrPI 3797 CACGTG CAC^GTG BciVI 22 GTATCC GTATCC(6/5) BclI 1087 TGATCA T^GATCA BsaAI 3797 CACGTG [CT]AC^GT[GA] BsaBI 2810 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1747 ACCGGT [AT]^CCGG[AT] BspHI 4387 TCATGA T^CATGA BspLU11I 2838 ACATGT A^CATGT BsrDI 1914 GCAATG GCAATG(2/0) BstBI 3966 TTCGAA TT^CGAA BstXI 3415 CCAAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Eam1105I 3291 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 4632 GATATC GAT^ATC FbaI 1087 TGATCA T^GATCA HindIII 1335 AAGCTT A^AGCTT HpaI 2531 GTTAAC GTT^AAC KasI 4757 GGCGCC G^GCGCC KpnI 3311 GGTACC GGTAC^C MamI 2810 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 1024 ACGCGT A^CGCGT NarI 4757 GGCGCC GG^CGCC NdeI 4657 CATATG CA^TATG NheI 594 GCTAGC G^CTAGC NspV 3966 TTCGAA TT^CGAA PinAI 1747 ACCGGT A^CCGGT PmaCI 3797 CACGTG CAC^GTG PmlI 3797 CACGTG CAC^GTG PshAI 1045 GACGCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 4036 AACGTT AA^CGTT RcaI 4387 TCATGA T^CATGA SacI 3762 GAGCTC GAGCT^C ScaI 4460 AGTACT AGT^ACT SfoI 4757 GGCGCC GGC^GCC SfuI 3966 TTCGAA TT^CGAA SgrAI 1746 CACCGGTG C[GA]^CCGG[CT]G SpeI 3566 ACTAGT A^CTAGT ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 1025 CGCG CG^CG 2823 CGCG AcyI 1045 GACGCC G[GA]^CG[CT]C 4757 GGCGCC Asp700I 132 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1593 GAATCTCTTC AspI 37 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4587 GACAGGGTC AvaI 2679 CCCGAG C^[CT]CG[GA]G 3248 CCCGAG BsaHI 1045 GACGCC G[GA]^CG[CT]C 4757 GGCGCC BsmBI 303 CGTCTC CGTCTC(1/5) 2250 GAGACG BsoBI 2679 CCCGAG C^[CT]CG[GA]G 3248 CCCGAG BstAPI 211 GCACTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3094 GCAGAGTGTGC BstUI 1025 CGCG CG^CG 2823 CGCG EcoNI 1170 CCTTCGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1784 CCTGGCTGAGG FauI 3838 CCCGC CCCGC(4/6) 2420 GCGGG Hin1I 1045 GACGCC G[GA]^CG[CT]C 4757 GGCGCC HincII 1236 GTTGAC GT[CT]^[GA]AC 2531 GTTAAC HindII 1236 GTTGAC GT[CT]^[GA]AC 2531 GTTAAC MvnI 1025 CGCG CG^CG 2823 CGCG NaeI 904 GCCGGC GCC^GGC 3385 GCCGGC NcoI 3178 CCATGG C^CATGG 3368 CCATGG NgoMIV 904 GCCGGC G^CCGGC 3385 GCCGGC PflFI 37 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4587 GACAGGGTC SexAI 985 ACCTGGT A^CC[AT]GGT 2209 ACCAGGT SphI 1477 GCATGC GCATG^C 3841 GCATGC TaqI 3967 TCGA T^CGA 4268 TCGA Tth111I 37 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4587 GACAGGGTC TthHB8I 3967 TCGA T^CGA 4268 TCGA XmnI 132 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1593 GAATCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII