##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccIII AflII Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
AviII AvrII BamHI BcgI BfrI
BglI BlnI BsaI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT22I FseI
FspI KspI MfeI MroI MunI
NotI NruI NsiI PacI PaeR7I
PmeI PshBI PvuI RsrII SacII
SalI SbfI SfiI SmaI SnaBI
Sse8387I SspBI SspI SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 3311 GGTACC G^GTACC
AccI 3508 GTAGAC GT^[AC][GT]AC
AclI 4036 AACGTT AA^CGTT
AgeI 1747 ACCGGT A^CCGGT
AhdI 3291 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 3311 GGTACC G^GTACC
AspEI 3291 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 4757 GGCGCC GGCGC^C
BbrPI 3797 CACGTG CAC^GTG
BciVI 22 GTATCC GTATCC(6/5)
BclI 1087 TGATCA T^GATCA
BsaAI 3797 CACGTG [CT]AC^GT[GA]
BsaBI 2810 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1747 ACCGGT [AT]^CCGG[AT]
BspHI 4387 TCATGA T^CATGA
BspLU11I 2838 ACATGT A^CATGT
BsrDI 1914 GCAATG GCAATG(2/0)
BstBI 3966 TTCGAA TT^CGAA
BstXI 3415 CCAAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I 3291 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 4632 GATATC GAT^ATC
FbaI 1087 TGATCA T^GATCA
HindIII 1335 AAGCTT A^AGCTT
HpaI 2531 GTTAAC GTT^AAC
KasI 4757 GGCGCC G^GCGCC
KpnI 3311 GGTACC GGTAC^C
MamI 2810 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1024 ACGCGT A^CGCGT
NarI 4757 GGCGCC GG^CGCC
NdeI 4657 CATATG CA^TATG
NheI 594 GCTAGC G^CTAGC
NspV 3966 TTCGAA TT^CGAA
PinAI 1747 ACCGGT A^CCGGT
PmaCI 3797 CACGTG CAC^GTG
PmlI 3797 CACGTG CAC^GTG
PshAI 1045 GACGCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4036 AACGTT AA^CGTT
RcaI 4387 TCATGA T^CATGA
SacI 3762 GAGCTC GAGCT^C
ScaI 4460 AGTACT AGT^ACT
SfoI 4757 GGCGCC GGC^GCC
SfuI 3966 TTCGAA TT^CGAA
SgrAI 1746 CACCGGTG C[GA]^CCGG[CT]G
SpeI 3566 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 1025 CGCG CG^CG
2823 CGCG
AcyI 1045 GACGCC G[GA]^CG[CT]C
4757 GGCGCC
Asp700I 132 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1593 GAATCTCTTC
AspI 37 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4587 GACAGGGTC
AvaI 2679 CCCGAG C^[CT]CG[GA]G
3248 CCCGAG
BsaHI 1045 GACGCC G[GA]^CG[CT]C
4757 GGCGCC
BsmBI 303 CGTCTC CGTCTC(1/5)
2250 GAGACG
BsoBI 2679 CCCGAG C^[CT]CG[GA]G
3248 CCCGAG
BstAPI 211 GCACTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3094 GCAGAGTGTGC
BstUI 1025 CGCG CG^CG
2823 CGCG
EcoNI 1170 CCTTCGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1784 CCTGGCTGAGG
FauI 3838 CCCGC CCCGC(4/6)
2420 GCGGG
Hin1I 1045 GACGCC G[GA]^CG[CT]C
4757 GGCGCC
HincII 1236 GTTGAC GT[CT]^[GA]AC
2531 GTTAAC
HindII 1236 GTTGAC GT[CT]^[GA]AC
2531 GTTAAC
MvnI 1025 CGCG CG^CG
2823 CGCG
NaeI 904 GCCGGC GCC^GGC
3385 GCCGGC
NcoI 3178 CCATGG C^CATGG
3368 CCATGG
NgoMIV 904 GCCGGC G^CCGGC
3385 GCCGGC
PflFI 37 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4587 GACAGGGTC
SexAI 985 ACCTGGT A^CC[AT]GGT
2209 ACCAGGT
SphI 1477 GCATGC GCATG^C
3841 GCATGC
TaqI 3967 TCGA T^CGA
4268 TCGA
Tth111I 37 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4587 GACAGGGTC
TthHB8I 3967 TCGA T^CGA
4268 TCGA
XmnI 132 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1593 GAATCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII