##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AviII BbeI BcgI BfrI BglII
BsiWI BspDI BspLU11I BsrBI BssHII
Bst1107I BstZ17I ClaI CpoI DraIII
DrdI EcoRI EcoRV EcoT22I FseI
FspI HpaI KasI KspI NarI
NheI NotI NruI NsiI PacI
PinAI PmeI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SnaBI SpeI Sse8387I
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 70 enzymes) #
###################################
AccIII 2868 TCCGGA T^CCGGA
AcyI 470 GACGCC G[GA]^CG[CT]C
AhdI 2213 GACCGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3684 GTGCAC G^TGCAC
AocI 174 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2332 AGCGCT AGC^GCT
ApaI 1804 GGGCCC GGGCC^C
ApaLI 3684 GTGCAC G^TGCAC
AseI 3433 ATTAAT AT^TAAT
AsnI 3433 ATTAAT AT^TAAT
AspEI 2213 GACCGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1982 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1153 CCTAGG C^CTAGG
BamHI 838 GGATCC G^GATCC
BbrPI 5443 CACGTG CAC^GTG
BbvCI 2593 CCTCAGC CCTCAGC(-5/-2)
BclI 2235 TGATCA T^GATCA
BlnI 1153 CCTAGG C^CTAGG
BsaAI 5443 CACGTG [CT]AC^GT[GA]
BsaBI 4919 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 470 GACGCC G[GA]^CG[CT]C
BseAI 2868 TCCGGA T^CCGGA
BsiEI 785 CGGCCG CG[GA][CT]^CG
BsmI 4352 GAATGC GAATGC(1/-1)
Bsp1407I 5439 TGTACA T^GTACA
BspEI 2868 TCCGGA T^CCGGA
BsrGI 5439 TGTACA T^GTACA
BstBI 4759 TTCGAA TT^CGAA
BstEII 4731 GGTCACC G^GT[ACGT]ACC
BstPI 4731 GGTCACC G^GT[ACGT]ACC
BstXI 898 CCACCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 174 CCTCAGG CC^T[ACGT]AGG
EagI 785 CGGCCG C^GGCCG
Eam1105I 2213 GACCGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 785 CGGCCG C^GGCCG
Eco47III 2332 AGCGCT AGC^GCT
Eco52I 785 CGGCCG C^GGCCG
Eco81I 174 CCTCAGG CC^T[ACGT]AGG
EcoO65I 4731 GGTCACC G^GT[ACGT]ACC
FauI 2830 GCGGG CCCGC(4/6)
FbaI 2235 TGATCA T^GATCA
Hin1I 470 GACGCC G[GA]^CG[CT]C
HindIII 2189 AAGCTT A^AGCTT
MamI 4919 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4774 CAATTG C^AATTG
MluI 4590 ACGCGT A^CGCGT
MroI 2868 TCCGGA T^CCGGA
MslI 5411 CATAGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 4774 CAATTG C^AATTG
NaeI 3044 GCCGGC GCC^GGC
NdeI 4967 CATATG CA^TATG
NgoMIV 3044 GCCGGC G^CCGGC
NspV 4759 TTCGAA TT^CGAA
PaeR7I 3275 CTCGAG C^TCGAG
PflFI 1982 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 5443 CACGTG CAC^GTG
PmlI 5443 CACGTG CAC^GTG
PshAI 797 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3433 ATTAAT AT^TAAT
ScaI 312 AGTACT AGT^ACT
SfuI 4759 TTCGAA TT^CGAA
SmaI 4418 CCCGGG CCC^GGG
SphI 1521 GCATGC GCATG^C
SspBI 5439 TGTACA T^GTACA
SspI 4854 AATATT AAT^ATT
StuI 1924 AGGCCT AGG^CCT
Tth111I 1982 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3275 CTCGAG C^TCGAG
XmaCI 4418 CCCGGG C^CCGGG
XmaI 4418 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 4591 CGCG CG^CG
5384 CGCG
AcsI 1079 GAATTT [GA]^AATT[CT]
4876 AAATTC
AflIII 4590 ACGCGT A^C[GA][CT]GT
5442 ACACGT
ApoI 1079 GAATTT [GA]^AATT[CT]
4876 AAATTC
BciVI 1120 GGATAC GTATCC(6/5)
5113 GGATAC
BsaWI 2214 ACCGGA [AT]^CCGG[AT]
2868 TCCGGA
BsmBI 5402 CGTCTC CGTCTC(1/5)
1319 GAGACG
BsrDI 4072 CATTGC GCAATG(2/0)
2712 GCAATG
BssSI 2296 CACGAG CACGAG(-5/-1)
1265 CTCGTG
BstUI 4591 CGCG CG^CG
5384 CGCG
DraI 1073 TTTAAA TTT^AAA
4873 TTTAAA
EcoNI 880 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2695 CCTCCAGCAGG
MvnI 4591 CGCG CG^CG
5384 CGCG
NspI 1521 GCATGC [GA]CATG^[CT]
3028 ACATGC
PflMI 202 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1351 CCAACAGGTGG
SacI 421 GAGCTC GAGCT^C
2690 GAGCTC
SapI 482 GAAGAGC GCTCTTC(1/4)
1331 GAAGAGC
Van91I 202 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1351 CCAACAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
Acc65I AccI AciI AfaI AluI
AlwI AlwNI Asp700I Asp718I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI