##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AccI AccII AclI AflII AhdI
ApaI AscI AseI AsnI AspEI
AspI AviII BbeI BbrPI BfrI
BglII BsaBI BsiWI BsmBI Bsp1407I
BspDI BspHI BsrGI BssHII Bst1107I
BstBI BstUI BstZ17I ClaI CpoI
Eam1105I EcoRV FseI FspI HpaI
KasI KspI MamI MluI MvnI
NarI NcoI NdeI NotI NruI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 1178 GACGTC GACGT^C
AcyI 1178 GACGTC G[GA]^CG[CT]C
AgeI 4066 ACCGGT A^CCGGT
Asp700I 974 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 529 CGAAGTCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1222 TGATCA T^GATCA
BglI 3467 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 4335 TACGTG [CT]AC^GT[GA]
BsaHI 1178 GACGTC G[GA]^CG[CT]C
BsiEI 47 CGGCCG CG[GA][CT]^CG
BstEII 2336 GGTGACC G^GT[ACGT]ACC
BstPI 2336 GGTGACC G^GT[ACGT]ACC
EagI 47 CGGCCG C^GGCCG
EclXI 47 CGGCCG C^GGCCG
Eco52I 47 CGGCCG C^GGCCG
EcoO65I 2336 GGTGACC G^GT[ACGT]ACC
FbaI 1222 TGATCA T^GATCA
Hin1I 1178 GACGTC G[GA]^CG[CT]C
HindIII 3325 AAGCTT A^AGCTT
MfeI 1757 CAATTG C^AATTG
MunI 1757 CAATTG C^AATTG
NaeI 4116 GCCGGC GCC^GGC
NgoMIV 4116 GCCGGC G^CCGGC
PinAI 4066 ACCGGT A^CCGGT
SapI 3579 GAAGAGC GCTCTTC(1/4)
ScaI 1123 AGTACT AGT^ACT
SspI 2437 AATATT AAT^ATT
XbaI 397 TCTAGA T^CTAGA
XmnI 974 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 1055 TCCGGA T^CCGGA
2814 TCCGGA
AflIII 242 ACATGT A^C[GA][CT]GT
3514 ACATGT
Aor51HI 2253 AGCGCT AGC^GCT
3155 AGCGCT
AvrII 448 CCTAGG C^CTAGG
3056 CCTAGG
BbsI 93 GAAGAC GAAGAC(2/6)
3933 GTCTTC
BlnI 448 CCTAGG C^CTAGG
3056 CCTAGG
BpuAI 93 GAAGAC GAAGAC(2/6)
3933 GTCTTC
BseAI 1055 TCCGGA T^CCGGA
2814 TCCGGA
BsmI 1522 GAATGC GAATGC(1/-1)
2497 GCATTC
BspEI 1055 TCCGGA T^CCGGA
2814 TCCGGA
BspLU11I 242 ACATGT A^CATGT
3514 ACATGT
BsrBI 367 CCGCTC CCGCTC(-3/-3)
4508 CCGCTC
BsrDI 1001 GCAATG GCAATG(2/0)
3290 GCAATG
DraIII 2571 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
3621 CACCGGGTG
DsaI 680 CCGTGG C^C[GA][CT]GG
1572 CCGTGG
Eco47III 2253 AGCGCT AGC^GCT
3155 AGCGCT
EcoT22I 2072 ATGCAT ATGCA^T
2217 ATGCAT
HgaI 272 GACGC GACGC(5/10)
2381 GCGTC
MroI 1055 TCCGGA T^CCGGA
2814 TCCGGA
NsiI 2072 ATGCAT ATGCA^T
2217 ATGCAT
PpuMI 52 GGGACCC [GA]G^G[AT]CC[CT]
2420 AGGTCCT
SexAI 2030 ACCAGGT A^CC[AT]GGT
2967 ACCAGGT
SmlI 862 CTCAAG C^T[CT][GA]AG
4545 CTCAAG
StuI 559 AGGCCT AGG^CCT
4456 AGGCCT
TfiI 689 GAATC G^A[AT]TC
2537 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AluI
Alw44I AlwI AlwNI AocI ApaLI
ApoI Asp718I AspHI AvaI AvaII
BalI BamHI BanI BanII BbvCI
BbvI BciVI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DrdI EaeI EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NheI NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII