##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       AccIII      AflII       AgeI        AhdI        
ApaI        AscI        AspEI       AspI        AviII       
BamHI       BbeI        BbrPI       BcgI        BclI        
BfrI        BglII       BsaAI       BseAI       BsiEI       
BsiWI       BspDI       BspEI       BsrFI       BssHII      
BssSI       Bst1107I    BstBI       BstZ17I     Cfr10I      
ClaI        CpoI        EagI        Eam1105I    EclXI       
Eco52I      EcoRV       EcoT22I     FbaI        FseI        
FspI        KasI        KspI        MluI        MroI        
NaeI        NarI        NgoMIV      NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PvuI        
RsrII       SacII       SalI        SapI        SbfI        
SexAI       SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SwaI        
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI          419  GTCTAC               GT^[AC][GT]AC
AclI         1757  AACGTT               AA^CGTT
AcyI          123  GACGCC               G[GA]^CG[CT]C
AflIII       2854  ACATGT               A^C[GA][CT]GT
Aor51HI      3883  AGCGCT               AGC^GCT
AseI         1966  ATTAAT               AT^TAAT
AsnI         1966  ATTAAT               AT^TAAT
AvaI         3385  CTCGGG               C^[CT]CG[GA]G
AvrII         101  CCTAGG               C^CTAGG
BalI          148  TGGCCA               TGG^CCA
BanII        4412  GAGCTC               G[GA]GC[CT]^C
BciVI        3203  GTATCC               GTATCC(6/5)
BglI          141  GCCGCCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          101  CCTAGG               C^CTAGG
BsaBI        3870  GATTTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         123  GACGCC               G[GA]^CG[CT]C
BsaWI         210  ACCGGA               [AT]^CCGG[AT]
BsmBI        1516  CGTCTC               CGTCTC(1/5)
BsoBI        3385  CTCGGG               C^[CT]CG[GA]G
Bsp1407I     4696  TGTACA               T^GTACA
BspHI        1289  TCATGA               T^CATGA
BspLU11I     2854  ACATGT               A^CATGT
BsrGI        4696  TGTACA               T^GTACA
BstAPI       1455  GCAGCCGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2589  GGTAACC              G^GT[ACGT]ACC
BstPI        2589  GGTAACC              G^GT[ACGT]ACC
Eco47III     3883  AGCGCT               AGC^GCT
EcoO65I      2589  GGTAACC              G^GT[ACGT]ACC
HaeII        3883  AGCGCT               [GA]GCGC^[CT]
Hin1I         123  GACGCC               G[GA]^CG[CT]C
HincII       1900  GTTAAC               GT[CT]^[GA]AC
HindII       1900  GTTAAC               GT[CT]^[GA]AC
HindIII       665  AAGCTT               A^AGCTT
HpaI         1900  GTTAAC               GTT^AAC
MamI         3870  GATTTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         148  TGGCCA               TGG^CCA
MscI          148  TGGCCA               TGG^CCA
NheI         2918  GCTAGC               G^CTAGC
PshAI        4454  GACCTCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1966  ATTAAT               AT^TAAT
Psp1406I     1757  AACGTT               AA^CGTT
PstI         3727  CTGCAG               CTGCA^G
RcaI         1289  TCATGA               T^CATGA
SacI         4412  GAGCTC               GAGCT^C
SfiI          140  GGCCGCCATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI        4696  TGTACA               T^GTACA
TaqI         3305  TCGA                 T^CGA
TthHB8I      3305  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

Acc65I         39  GGTACC               G^GTACC
             1635  GGTACC              
AccII         131  CGCG                 CG^CG
              245  CGCG                
Alw44I       2843  GTGCAC               G^TGCAC
             3784  GTGCAC              
AlwNI         945  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1090  CAGCTCCTG           
ApaLI        2843  GTGCAC               G^TGCAC
             3784  GTGCAC              
Asp718I        39  GGTACC               G^GTACC
             1635  GGTACC              
BcnI           36  CCCGG                CC^[GC]GG
              201  CCCGG               
BlpI         3321  GCTTAGC              GC^T[ACGT]AGC
             4248  GCTTAGC             
Bpu1102I     3321  GCTTAGC              GC^T[ACGT]AGC
             4248  GCTTAGC             
BseRI        1213  CTCCTC               GAGGAG(10/8)
             1808  CTCCTC              
BsgI          391  GTGCAG               GTGCAG(16/14)
             1696  GTGCAG              
BstUI         131  CGCG                 CG^CG
              245  CGCG                
BstXI          54  CCAGCTATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              187  CCACCACTGTGG        
CelII        3321  GCTTAGC              GC^T[ACGT]AGC
             4248  GCTTAGC             
CfoI          132  GCGC                 GCG^C
             3884  GCGC                
DraIII       2135  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3776  CACAATGTG           
DrdI           90  GACAGAAGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              239  GACCGTCGCGTC        
EaeI          148  TGGCCA               [CT]^GGCC[GA]
             3314  TGGCCG              
EcoNI        1497  CCTGCTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3702  CCTTGACAAGG         
HhaI          132  GCGC                 GCG^C
             3884  GCGC                
HinP1I        132  GCGC                 G^CGC
             3884  GCGC                
KpnI           39  GGTACC               GGTAC^C
             1635  GGTACC              
MvnI          131  CGCG                 CG^CG
              245  CGCG                
NciI           36  CCCGG                CC^[GC]GG
              201  CCCGG               
NdeI         2419  CATATG               CA^TATG
             5086  CATATG              
PflMI        1387  CCAAGAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4910  CCAGACTATGG         
PpuMI         174  GGGACCC              [GA]G^G[AT]CC[CT]
             1791  AGGACCC             
ScaI         2397  AGTACT               AGT^ACT
             3592  AGTACT              
Van91I       1387  CCAAGAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4910  CCAGACTATGG         


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AocI        ApoI        Asp700I     AspHI       AvaII       
BanI        BbsI        BbvCI       BbvI        BfaI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BspMI       BsrBI       BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Bsu36I      Cac8I       Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EarI        Eco81I      
EcoO109I    EcoRI       EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MfeI        MflI        MnlI        MseI        MslI        
MspA1I      MspI        MunI        MvaI        MwoI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspI        
StuI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XhoII       XmnI