##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
ApaI AscI AspEI AspI AviII
BamHI BbeI BbrPI BcgI BclI
BfrI BglII BsaAI BseAI BsiEI
BsiWI BspDI BspEI BsrFI BssHII
BssSI Bst1107I BstBI BstZ17I Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoRV EcoT22I FbaI FseI
FspI KasI KspI MluI MroI
NaeI NarI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PvuI
RsrII SacII SalI SapI SbfI
SexAI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 419 GTCTAC GT^[AC][GT]AC
AclI 1757 AACGTT AA^CGTT
AcyI 123 GACGCC G[GA]^CG[CT]C
AflIII 2854 ACATGT A^C[GA][CT]GT
Aor51HI 3883 AGCGCT AGC^GCT
AseI 1966 ATTAAT AT^TAAT
AsnI 1966 ATTAAT AT^TAAT
AvaI 3385 CTCGGG C^[CT]CG[GA]G
AvrII 101 CCTAGG C^CTAGG
BalI 148 TGGCCA TGG^CCA
BanII 4412 GAGCTC G[GA]GC[CT]^C
BciVI 3203 GTATCC GTATCC(6/5)
BglI 141 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 101 CCTAGG C^CTAGG
BsaBI 3870 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 123 GACGCC G[GA]^CG[CT]C
BsaWI 210 ACCGGA [AT]^CCGG[AT]
BsmBI 1516 CGTCTC CGTCTC(1/5)
BsoBI 3385 CTCGGG C^[CT]CG[GA]G
Bsp1407I 4696 TGTACA T^GTACA
BspHI 1289 TCATGA T^CATGA
BspLU11I 2854 ACATGT A^CATGT
BsrGI 4696 TGTACA T^GTACA
BstAPI 1455 GCAGCCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2589 GGTAACC G^GT[ACGT]ACC
BstPI 2589 GGTAACC G^GT[ACGT]ACC
Eco47III 3883 AGCGCT AGC^GCT
EcoO65I 2589 GGTAACC G^GT[ACGT]ACC
HaeII 3883 AGCGCT [GA]GCGC^[CT]
Hin1I 123 GACGCC G[GA]^CG[CT]C
HincII 1900 GTTAAC GT[CT]^[GA]AC
HindII 1900 GTTAAC GT[CT]^[GA]AC
HindIII 665 AAGCTT A^AGCTT
HpaI 1900 GTTAAC GTT^AAC
MamI 3870 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 148 TGGCCA TGG^CCA
MscI 148 TGGCCA TGG^CCA
NheI 2918 GCTAGC G^CTAGC
PshAI 4454 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1966 ATTAAT AT^TAAT
Psp1406I 1757 AACGTT AA^CGTT
PstI 3727 CTGCAG CTGCA^G
RcaI 1289 TCATGA T^CATGA
SacI 4412 GAGCTC GAGCT^C
SfiI 140 GGCCGCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 4696 TGTACA T^GTACA
TaqI 3305 TCGA T^CGA
TthHB8I 3305 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 39 GGTACC G^GTACC
1635 GGTACC
AccII 131 CGCG CG^CG
245 CGCG
Alw44I 2843 GTGCAC G^TGCAC
3784 GTGCAC
AlwNI 945 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1090 CAGCTCCTG
ApaLI 2843 GTGCAC G^TGCAC
3784 GTGCAC
Asp718I 39 GGTACC G^GTACC
1635 GGTACC
BcnI 36 CCCGG CC^[GC]GG
201 CCCGG
BlpI 3321 GCTTAGC GC^T[ACGT]AGC
4248 GCTTAGC
Bpu1102I 3321 GCTTAGC GC^T[ACGT]AGC
4248 GCTTAGC
BseRI 1213 CTCCTC GAGGAG(10/8)
1808 CTCCTC
BsgI 391 GTGCAG GTGCAG(16/14)
1696 GTGCAG
BstUI 131 CGCG CG^CG
245 CGCG
BstXI 54 CCAGCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
187 CCACCACTGTGG
CelII 3321 GCTTAGC GC^T[ACGT]AGC
4248 GCTTAGC
CfoI 132 GCGC GCG^C
3884 GCGC
DraIII 2135 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3776 CACAATGTG
DrdI 90 GACAGAAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
239 GACCGTCGCGTC
EaeI 148 TGGCCA [CT]^GGCC[GA]
3314 TGGCCG
EcoNI 1497 CCTGCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3702 CCTTGACAAGG
HhaI 132 GCGC GCG^C
3884 GCGC
HinP1I 132 GCGC G^CGC
3884 GCGC
KpnI 39 GGTACC GGTAC^C
1635 GGTACC
MvnI 131 CGCG CG^CG
245 CGCG
NciI 36 CCCGG CC^[GC]GG
201 CCCGG
NdeI 2419 CATATG CA^TATG
5086 CATATG
PflMI 1387 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4910 CCAGACTATGG
PpuMI 174 GGGACCC [GA]G^G[AT]CC[CT]
1791 AGGACCC
ScaI 2397 AGTACT AGT^ACT
3592 AGTACT
Van91I 1387 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4910 CCAGACTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI Asp700I AspHI AvaII
BanI BbsI BbvCI BbvI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrBI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EarI Eco81I
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspA1I MspI MunI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI