################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII AccIII AflII AgeI AhdI ApaI AscI AspEI AspI AviII BamHI BbeI BbrPI BcgI BclI BfrI BglII BsaAI BseAI BsiEI BsiWI BspDI BspEI BsrFI BssHII BssSI Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FbaI FseI FspI KasI KspI MluI MroI NaeI NarI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PvuI RsrII SacII SalI SapI SbfI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 419 GTCTAC GT^[AC][GT]AC AclI 1757 AACGTT AA^CGTT AcyI 123 GACGCC G[GA]^CG[CT]C AflIII 2854 ACATGT A^C[GA][CT]GT Aor51HI 3883 AGCGCT AGC^GCT AseI 1966 ATTAAT AT^TAAT AsnI 1966 ATTAAT AT^TAAT AvaI 3385 CTCGGG C^[CT]CG[GA]G AvrII 101 CCTAGG C^CTAGG BalI 148 TGGCCA TGG^CCA BanII 4412 GAGCTC G[GA]GC[CT]^C BciVI 3203 GTATCC GTATCC(6/5) BglI 141 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 101 CCTAGG C^CTAGG BsaBI 3870 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 123 GACGCC G[GA]^CG[CT]C BsaWI 210 ACCGGA [AT]^CCGG[AT] BsmBI 1516 CGTCTC CGTCTC(1/5) BsoBI 3385 CTCGGG C^[CT]CG[GA]G Bsp1407I 4696 TGTACA T^GTACA BspHI 1289 TCATGA T^CATGA BspLU11I 2854 ACATGT A^CATGT BsrGI 4696 TGTACA T^GTACA BstAPI 1455 GCAGCCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2589 GGTAACC G^GT[ACGT]ACC BstPI 2589 GGTAACC G^GT[ACGT]ACC Eco47III 3883 AGCGCT AGC^GCT EcoO65I 2589 GGTAACC G^GT[ACGT]ACC HaeII 3883 AGCGCT [GA]GCGC^[CT] Hin1I 123 GACGCC G[GA]^CG[CT]C HincII 1900 GTTAAC GT[CT]^[GA]AC HindII 1900 GTTAAC GT[CT]^[GA]AC HindIII 665 AAGCTT A^AGCTT HpaI 1900 GTTAAC GTT^AAC MamI 3870 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 148 TGGCCA TGG^CCA MscI 148 TGGCCA TGG^CCA NheI 2918 GCTAGC G^CTAGC PshAI 4454 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1966 ATTAAT AT^TAAT Psp1406I 1757 AACGTT AA^CGTT PstI 3727 CTGCAG CTGCA^G RcaI 1289 TCATGA T^CATGA SacI 4412 GAGCTC GAGCT^C SfiI 140 GGCCGCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 4696 TGTACA T^GTACA TaqI 3305 TCGA T^CGA TthHB8I 3305 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 39 GGTACC G^GTACC 1635 GGTACC AccII 131 CGCG CG^CG 245 CGCG Alw44I 2843 GTGCAC G^TGCAC 3784 GTGCAC AlwNI 945 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 1090 CAGCTCCTG ApaLI 2843 GTGCAC G^TGCAC 3784 GTGCAC Asp718I 39 GGTACC G^GTACC 1635 GGTACC BcnI 36 CCCGG CC^[GC]GG 201 CCCGG BlpI 3321 GCTTAGC GC^T[ACGT]AGC 4248 GCTTAGC Bpu1102I 3321 GCTTAGC GC^T[ACGT]AGC 4248 GCTTAGC BseRI 1213 CTCCTC GAGGAG(10/8) 1808 CTCCTC BsgI 391 GTGCAG GTGCAG(16/14) 1696 GTGCAG BstUI 131 CGCG CG^CG 245 CGCG BstXI 54 CCAGCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 187 CCACCACTGTGG CelII 3321 GCTTAGC GC^T[ACGT]AGC 4248 GCTTAGC CfoI 132 GCGC GCG^C 3884 GCGC DraIII 2135 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG 3776 CACAATGTG DrdI 90 GACAGAAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 239 GACCGTCGCGTC EaeI 148 TGGCCA [CT]^GGCC[GA] 3314 TGGCCG EcoNI 1497 CCTGCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3702 CCTTGACAAGG HhaI 132 GCGC GCG^C 3884 GCGC HinP1I 132 GCGC G^CGC 3884 GCGC KpnI 39 GGTACC GGTAC^C 1635 GGTACC MvnI 131 CGCG CG^CG 245 CGCG NciI 36 CCCGG CC^[GC]GG 201 CCCGG NdeI 2419 CATATG CA^TATG 5086 CATATG PflMI 1387 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4910 CCAGACTATGG PpuMI 174 GGGACCC [GA]G^G[AT]CC[CT] 1791 AGGACCC ScaI 2397 AGTACT AGT^ACT 3592 AGTACT Van91I 1387 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4910 CCAGACTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AocI ApoI Asp700I AspHI AvaII BanI BbsI BbvCI BbvI BfaI BmyI BpmI BpuAI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI