##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
Aor51HI ApaI ApaLI AscI AspI
AvrII BbrPI BclI BfrI BlnI
BsiEI BsiWI BspDI BssHII Bst1107I
BstZ17I ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoRI
EcoRV FbaI HindIII HpaI KspI
MluI NheI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SgrAI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 1544 GTCTAC GT^[AC][GT]AC
AccII 2291 CGCG CG^CG
AccIII 165 TCCGGA T^CCGGA
AflIII 1645 ACATGT A^C[GA][CT]GT
AhdI 4529 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 4856 ATTAAT AT^TAAT
AsnI 4856 ATTAAT AT^TAAT
Asp700I 491 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4529 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2916 TGCGCA TGC^GCA
BamHI 4225 GGATCC G^GATCC
BcgI 2994 GCAGCTTCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2987 GGATAC GTATCC(6/5)
BglII 3606 AGATCT A^GATCT
BsaAI 481 TACGTA [CT]AC^GT[GA]
BsaBI 3730 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2181 GGTCTC GGTCTC(1/5)
BsaWI 165 TCCGGA [AT]^CCGG[AT]
BseAI 165 TCCGGA T^CCGGA
BsmBI 3861 GAGACG CGTCTC(1/5)
BsmI 4402 GCATTC GAATGC(1/-1)
BspEI 165 TCCGGA T^CCGGA
BspLU11I 1645 ACATGT A^CATGT
BsrBI 1537 GAGCGG CCGCTC(-3/-3)
BsrDI 458 GCAATG GCAATG(2/0)
BstBI 443 TTCGAA TT^CGAA
BstUI 2291 CGCG CG^CG
Eam1105I 4529 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1392 ATGCAT ATGCA^T
FseI 4360 GGCCGGCC GGCCGG^CC
FspI 2916 TGCGCA TGC^GCA
MamI 3730 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4165 CAATTG C^AATTG
MroI 165 TCCGGA T^CCGGA
MunI 4165 CAATTG C^AATTG
MvnI 2291 CGCG CG^CG
NdeI 748 CATATG CA^TATG
NsiI 1392 ATGCAT ATGCA^T
NspV 443 TTCGAA TT^CGAA
PflMI 3362 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3115 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4856 ATTAAT AT^TAAT
SexAI 1105 ACCTGGT A^CC[AT]GGT
SfiI 2166 GGCCCGTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 443 TTCGAA TT^CGAA
SnaBI 481 TACGTA TAC^GTA
SspI 3627 AATATT AAT^ATT
StuI 2759 AGGCCT AGG^CCT
Van91I 3362 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 491 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
Bsp1407I 1503 TGTACA T^GTACA
2882 TGTACA
BspHI 1217 TCATGA T^CATGA
1437 TCATGA
BspMI 2093 ACCTGC ACCTGC(4/8)
2277 GCAGGT
BsrGI 1503 TGTACA T^GTACA
2882 TGTACA
DraI 718 TTTAAA TTT^AAA
4699 TTTAAA
EcoNI 3117 CCTGTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4532 CCTGGGTCAGG
HincII 1351 GTCAAC GT[CT]^[GA]AC
3039 GTTGAC
HindII 1351 GTCAAC GT[CT]^[GA]AC
3039 GTTGAC
NaeI 2830 GCCGGC GCC^GGC
4361 GCCGGC
NgoMIV 2830 GCCGGC G^CCGGC
4361 GCCGGC
RcaI 1217 TCATGA T^CATGA
1437 TCATGA
SspBI 1503 TGTACA T^GTACA
2882 TGTACA
XcmI 3199 CCACTGCTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3383 CCAGACAGGTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
Acc65I AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BssSI BstAPI
BstEII BstF5I BstNI BstPI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmaCI XmaI