################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII AclI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AspI AvrII BbrPI BclI BfrI BlnI BsiEI BsiWI BspDI BssHII Bst1107I BstZ17I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoRI EcoRV FbaI HindIII HpaI KspI MluI NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SgrAI SpeI SphI Sse8387I SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 1544 GTCTAC GT^[AC][GT]AC AccII 2291 CGCG CG^CG AccIII 165 TCCGGA T^CCGGA AflIII 1645 ACATGT A^C[GA][CT]GT AhdI 4529 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 4856 ATTAAT AT^TAAT AsnI 4856 ATTAAT AT^TAAT Asp700I 491 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 4529 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2916 TGCGCA TGC^GCA BamHI 4225 GGATCC G^GATCC BcgI 2994 GCAGCTTCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2987 GGATAC GTATCC(6/5) BglII 3606 AGATCT A^GATCT BsaAI 481 TACGTA [CT]AC^GT[GA] BsaBI 3730 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2181 GGTCTC GGTCTC(1/5) BsaWI 165 TCCGGA [AT]^CCGG[AT] BseAI 165 TCCGGA T^CCGGA BsmBI 3861 GAGACG CGTCTC(1/5) BsmI 4402 GCATTC GAATGC(1/-1) BspEI 165 TCCGGA T^CCGGA BspLU11I 1645 ACATGT A^CATGT BsrBI 1537 GAGCGG CCGCTC(-3/-3) BsrDI 458 GCAATG GCAATG(2/0) BstBI 443 TTCGAA TT^CGAA BstUI 2291 CGCG CG^CG Eam1105I 4529 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 1392 ATGCAT ATGCA^T FseI 4360 GGCCGGCC GGCCGG^CC FspI 2916 TGCGCA TGC^GCA MamI 3730 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4165 CAATTG C^AATTG MroI 165 TCCGGA T^CCGGA MunI 4165 CAATTG C^AATTG MvnI 2291 CGCG CG^CG NdeI 748 CATATG CA^TATG NsiI 1392 ATGCAT ATGCA^T NspV 443 TTCGAA TT^CGAA PflMI 3362 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 3115 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4856 ATTAAT AT^TAAT SexAI 1105 ACCTGGT A^CC[AT]GGT SfiI 2166 GGCCCGTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 443 TTCGAA TT^CGAA SnaBI 481 TACGTA TAC^GTA SspI 3627 AATATT AAT^ATT StuI 2759 AGGCCT AGG^CCT Van91I 3362 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 491 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### Bsp1407I 1503 TGTACA T^GTACA 2882 TGTACA BspHI 1217 TCATGA T^CATGA 1437 TCATGA BspMI 2093 ACCTGC ACCTGC(4/8) 2277 GCAGGT BsrGI 1503 TGTACA T^GTACA 2882 TGTACA DraI 718 TTTAAA TTT^AAA 4699 TTTAAA EcoNI 3117 CCTGTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4532 CCTGGGTCAGG HincII 1351 GTCAAC GT[CT]^[GA]AC 3039 GTTGAC HindII 1351 GTCAAC GT[CT]^[GA]AC 3039 GTTGAC NaeI 2830 GCCGGC GCC^GGC 4361 GCCGGC NgoMIV 2830 GCCGGC G^CCGGC 4361 GCCGGC RcaI 1217 TCATGA T^CATGA 1437 TCATGA SspBI 1503 TGTACA T^GTACA 2882 TGTACA XcmI 3199 CCACTGCTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3383 CCAGACAGGTTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 133 enzymes) # ######################################### Acc65I AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApoI Asp718I AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI