##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI Asp718I AspEI AvaI
AviII AvrII BamHI BanI BbeI
BbvCI BcgI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BseAI BsiEI BsiWI BsoBI Bsp1407I
BspEI BspLU11I BsrFI BsrGI BssHII
BssSI BstBI CelII CfoI Cfr10I
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoT22I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KpnI MamI
MfeI MluI MroI MunI NaeI
NarI NciI NcoI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacI SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccII 1820 CGCG CG^CG
Alw44I 437 GTGCAC G^TGCAC
ApaLI 437 GTGCAC G^TGCAC
AseI 2338 ATTAAT AT^TAAT
AsnI 2338 ATTAAT AT^TAAT
BanII 3943 GGGCTC G[GA]GC[CT]^C
BbrPI 3692 CACGTG CAC^GTG
BglII 3235 AGATCT A^GATCT
BsaAI 3692 CACGTG [CT]AC^GT[GA]
BsmBI 3243 CGTCTC CGTCTC(1/5)
BspDI 3208 ATCGAT AT^CGAT
BstEII 1750 GGTAACC G^GT[ACGT]ACC
BstPI 1750 GGTAACC G^GT[ACGT]ACC
BstUI 1820 CGCG CG^CG
ClaI 3208 ATCGAT AT^CGAT
DsaI 1819 CCGCGG C^C[GA][CT]GG
EcoO65I 1750 GGTAACC G^GT[ACGT]ACC
EcoRV 3634 GATATC GAT^ATC
FauI 1821 GCGGG CCCGC(4/6)
KspI 1819 CCGCGG CCGC^GG
MvnI 1820 CGCG CG^CG
NdeI 3563 CATATG CA^TATG
NheI 1891 GCTAGC G^CTAGC
PmaCI 3692 CACGTG CAC^GTG
PmlI 3692 CACGTG CAC^GTG
PshBI 2338 ATTAAT AT^TAAT
SacII 1819 CCGCGG CCGC^GG
ScaI 200 AGTACT AGT^ACT
SphI 3328 GCATGC GCATG^C
StuI 3550 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AlwI 164 GATCC GGATC(4/5)
2965 GATCC
AocI 1067 CCTGAGG CC^T[ACGT]AGG
2905 CCTGAGG
Asp700I 1618 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2444 GAACTCGTTC
BalI 344 TGGCCA TGG^CCA
3538 TGGCCA
BbsI 773 GAAGAC GAAGAC(2/6)
2654 GAAGAC
BclI 1795 TGATCA T^GATCA
2799 TGATCA
BpuAI 773 GAAGAC GAAGAC(2/6)
2654 GAAGAC
BsaI 430 GAGACC GGTCTC(1/5)
3152 GAGACC
BsaWI 1380 TCCGGT [AT]^CCGG[AT]
3318 TCCGGT
BseRI 761 GAGGAG GAGGAG(10/8)
3110 GAGGAG
BsgI 136 GTGCAG GTGCAG(16/14)
1220 GTGCAG
BsmI 1134 GAATGC GAATGC(1/-1)
1845 GCATTC
BsrBI 2092 CCGCTC CCGCTC(-3/-3)
1283 GAGCGG
Bst1107I 3845 GTATAC GTA^TAC
4092 GTATAC
BstAPI 2783 GCAAACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2882 GCAAGTGCTGC
BstYI 163 AGATCC [GA]^GATC[CT]
3235 AGATCT
BstZ17I 3845 GTATAC GTA^TAC
4092 GTATAC
Bsu36I 1067 CCTGAGG CC^T[ACGT]AGG
2905 CCTGAGG
EarI 641 GAAGAG CTCTTC(1/4)
758 GAAGAG
Eco81I 1067 CCTGAGG CC^T[ACGT]AGG
2905 CCTGAGG
EcoNI 1447 CCTACATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2749 CCTTCTAAAGG
EcoRI 3425 GAATTC G^AATTC
3811 GAATTC
EcoT14I 1471 CCTTGG C^C[AT][AT]GG
3393 CCAAGG
FbaI 1795 TGATCA T^GATCA
2799 TGATCA
HapII 1381 CCGG C^CGG
3319 CCGG
HindIII 2642 AAGCTT A^AGCTT
3492 AAGCTT
HpaII 1381 CCGG C^CGG
3319 CCGG
Ksp632I 641 GAAGAG CTCTTC(1/4)
758 GAAGAG
MflI 163 AGATCC [GA]^GATC[CT]
3235 AGATCT
MluNI 344 TGGCCA TGG^CCA
3538 TGGCCA
MscI 344 TGGCCA TGG^CCA
3538 TGGCCA
MspI 1381 CCGG C^CGG
3319 CCGG
PflMI 1261 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2586 CCAAACTTTGG
PvuII 131 CAGCTG CAG^CTG
1249 CAGCTG
SspI 2275 AATATT AAT^ATT
2720 AATATT
StyI 1471 CCTTGG C^C[AT][AT]GG
3393 CCAAGG
Van91I 1261 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2586 CCAAACTTTGG
XcmI 672 CCATATCTACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1596 CCACATATTTCATGG
XhoII 163 AGATCC [GA]^GATC[CT]
3235 AGATCT
XmnI 1618 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2444 GAACTCGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwNI ApoI AspHI AspI AvaII
BbvI BciVI BfaI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI DpnII DraI
DraII EaeI EcoO109I EcoRII Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PflFI PleI PpuMI
PstI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I