##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Aor51HI     
ApaI        AscI        Asp718I     AspEI       AvaI        
AviII       AvrII       BamHI       BanI        BbeI        
BbvCI       BcgI        BcnI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BseAI       BsiEI       BsiWI       BsoBI       Bsp1407I    
BspEI       BspLU11I    BsrFI       BsrGI       BssHII      
BssSI       BstBI       CelII       CfoI        Cfr10I      
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoT22I     FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HpaI        KasI        KpnI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NciI        NcoI        NgoMIV      NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshAI       Psp1406I    PvuI        
RsrII       SacI        SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
SwaI        XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AccII        1820  CGCG                 CG^CG
Alw44I        437  GTGCAC               G^TGCAC
ApaLI         437  GTGCAC               G^TGCAC
AseI         2338  ATTAAT               AT^TAAT
AsnI         2338  ATTAAT               AT^TAAT
BanII        3943  GGGCTC               G[GA]GC[CT]^C
BbrPI        3692  CACGTG               CAC^GTG
BglII        3235  AGATCT               A^GATCT
BsaAI        3692  CACGTG               [CT]AC^GT[GA]
BsmBI        3243  CGTCTC               CGTCTC(1/5)
BspDI        3208  ATCGAT               AT^CGAT
BstEII       1750  GGTAACC              G^GT[ACGT]ACC
BstPI        1750  GGTAACC              G^GT[ACGT]ACC
BstUI        1820  CGCG                 CG^CG
ClaI         3208  ATCGAT               AT^CGAT
DsaI         1819  CCGCGG               C^C[GA][CT]GG
EcoO65I      1750  GGTAACC              G^GT[ACGT]ACC
EcoRV        3634  GATATC               GAT^ATC
FauI         1821  GCGGG                CCCGC(4/6)
KspI         1819  CCGCGG               CCGC^GG
MvnI         1820  CGCG                 CG^CG
NdeI         3563  CATATG               CA^TATG
NheI         1891  GCTAGC               G^CTAGC
PmaCI        3692  CACGTG               CAC^GTG
PmlI         3692  CACGTG               CAC^GTG
PshBI        2338  ATTAAT               AT^TAAT
SacII        1819  CCGCGG               CCGC^GG
ScaI          200  AGTACT               AGT^ACT
SphI         3328  GCATGC               GCATG^C
StuI         3550  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   40 enzymes) #
###################################

AlwI          164  GATCC                GGATC(4/5)
             2965  GATCC               
AocI         1067  CCTGAGG              CC^T[ACGT]AGG
             2905  CCTGAGG             
Asp700I      1618  GAACCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2444  GAACTCGTTC          
BalI          344  TGGCCA               TGG^CCA
             3538  TGGCCA              
BbsI          773  GAAGAC               GAAGAC(2/6)
             2654  GAAGAC              
BclI         1795  TGATCA               T^GATCA
             2799  TGATCA              
BpuAI         773  GAAGAC               GAAGAC(2/6)
             2654  GAAGAC              
BsaI          430  GAGACC               GGTCTC(1/5)
             3152  GAGACC              
BsaWI        1380  TCCGGT               [AT]^CCGG[AT]
             3318  TCCGGT              
BseRI         761  GAGGAG               GAGGAG(10/8)
             3110  GAGGAG              
BsgI          136  GTGCAG               GTGCAG(16/14)
             1220  GTGCAG              
BsmI         1134  GAATGC               GAATGC(1/-1)
             1845  GCATTC              
BsrBI        2092  CCGCTC               CCGCTC(-3/-3)
             1283  GAGCGG              
Bst1107I     3845  GTATAC               GTA^TAC
             4092  GTATAC              
BstAPI       2783  GCAAACTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2882  GCAAGTGCTGC         
BstYI         163  AGATCC               [GA]^GATC[CT]
             3235  AGATCT              
BstZ17I      3845  GTATAC               GTA^TAC
             4092  GTATAC              
Bsu36I       1067  CCTGAGG              CC^T[ACGT]AGG
             2905  CCTGAGG             
EarI          641  GAAGAG               CTCTTC(1/4)
              758  GAAGAG              
Eco81I       1067  CCTGAGG              CC^T[ACGT]AGG
             2905  CCTGAGG             
EcoNI        1447  CCTACATCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2749  CCTTCTAAAGG         
EcoRI        3425  GAATTC               G^AATTC
             3811  GAATTC              
EcoT14I      1471  CCTTGG               C^C[AT][AT]GG
             3393  CCAAGG              
FbaI         1795  TGATCA               T^GATCA
             2799  TGATCA              
HapII        1381  CCGG                 C^CGG
             3319  CCGG                
HindIII      2642  AAGCTT               A^AGCTT
             3492  AAGCTT              
HpaII        1381  CCGG                 C^CGG
             3319  CCGG                
Ksp632I       641  GAAGAG               CTCTTC(1/4)
              758  GAAGAG              
MflI          163  AGATCC               [GA]^GATC[CT]
             3235  AGATCT              
MluNI         344  TGGCCA               TGG^CCA
             3538  TGGCCA              
MscI          344  TGGCCA               TGG^CCA
             3538  TGGCCA              
MspI         1381  CCGG                 C^CGG
             3319  CCGG                
PflMI        1261  CCAGAATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2586  CCAAACTTTGG         
PvuII         131  CAGCTG               CAG^CTG
             1249  CAGCTG              
SspI         2275  AATATT               AAT^ATT
             2720  AATATT              
StyI         1471  CCTTGG               C^C[AT][AT]GG
             3393  CCAAGG              
Van91I       1261  CCAGAATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2586  CCAAACTTTGG         
XcmI          672  CCATATCTACAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1596  CCACATATTTCATGG     
XhoII         163  AGATCC               [GA]^GATC[CT]
             3235  AGATCT              
XmnI         1618  GAACCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2444  GAACTCGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwNI       ApoI        AspHI       AspI        AvaII       
BbvI        BciVI       BfaI        BmyI        BpmI        
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspHI       BspMI       BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
Cac8I       Cfr13I      DdeI        DpnII       DraI        
DraII       EaeI        EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HincII      HindII      HinfI       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PflFI       PleI        PpuMI       
PstI        RcaI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I