################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AvaI AviII AvrII BamHI BanI BbeI BbvCI BcgI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsiEI BsiWI BsoBI Bsp1407I BspEI BspLU11I BsrFI BsrGI BssHII BssSI BstBI CelII CfoI Cfr10I CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI MamI MfeI MluI MroI MunI NaeI NarI NciI NcoI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI Psp1406I PvuI RsrII SacI SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AccII 1820 CGCG CG^CG Alw44I 437 GTGCAC G^TGCAC ApaLI 437 GTGCAC G^TGCAC AseI 2338 ATTAAT AT^TAAT AsnI 2338 ATTAAT AT^TAAT BanII 3943 GGGCTC G[GA]GC[CT]^C BbrPI 3692 CACGTG CAC^GTG BglII 3235 AGATCT A^GATCT BsaAI 3692 CACGTG [CT]AC^GT[GA] BsmBI 3243 CGTCTC CGTCTC(1/5) BspDI 3208 ATCGAT AT^CGAT BstEII 1750 GGTAACC G^GT[ACGT]ACC BstPI 1750 GGTAACC G^GT[ACGT]ACC BstUI 1820 CGCG CG^CG ClaI 3208 ATCGAT AT^CGAT DsaI 1819 CCGCGG C^C[GA][CT]GG EcoO65I 1750 GGTAACC G^GT[ACGT]ACC EcoRV 3634 GATATC GAT^ATC FauI 1821 GCGGG CCCGC(4/6) KspI 1819 CCGCGG CCGC^GG MvnI 1820 CGCG CG^CG NdeI 3563 CATATG CA^TATG NheI 1891 GCTAGC G^CTAGC PmaCI 3692 CACGTG CAC^GTG PmlI 3692 CACGTG CAC^GTG PshBI 2338 ATTAAT AT^TAAT SacII 1819 CCGCGG CCGC^GG ScaI 200 AGTACT AGT^ACT SphI 3328 GCATGC GCATG^C StuI 3550 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AlwI 164 GATCC GGATC(4/5) 2965 GATCC AocI 1067 CCTGAGG CC^T[ACGT]AGG 2905 CCTGAGG Asp700I 1618 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2444 GAACTCGTTC BalI 344 TGGCCA TGG^CCA 3538 TGGCCA BbsI 773 GAAGAC GAAGAC(2/6) 2654 GAAGAC BclI 1795 TGATCA T^GATCA 2799 TGATCA BpuAI 773 GAAGAC GAAGAC(2/6) 2654 GAAGAC BsaI 430 GAGACC GGTCTC(1/5) 3152 GAGACC BsaWI 1380 TCCGGT [AT]^CCGG[AT] 3318 TCCGGT BseRI 761 GAGGAG GAGGAG(10/8) 3110 GAGGAG BsgI 136 GTGCAG GTGCAG(16/14) 1220 GTGCAG BsmI 1134 GAATGC GAATGC(1/-1) 1845 GCATTC BsrBI 2092 CCGCTC CCGCTC(-3/-3) 1283 GAGCGG Bst1107I 3845 GTATAC GTA^TAC 4092 GTATAC BstAPI 2783 GCAAACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2882 GCAAGTGCTGC BstYI 163 AGATCC [GA]^GATC[CT] 3235 AGATCT BstZ17I 3845 GTATAC GTA^TAC 4092 GTATAC Bsu36I 1067 CCTGAGG CC^T[ACGT]AGG 2905 CCTGAGG EarI 641 GAAGAG CTCTTC(1/4) 758 GAAGAG Eco81I 1067 CCTGAGG CC^T[ACGT]AGG 2905 CCTGAGG EcoNI 1447 CCTACATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2749 CCTTCTAAAGG EcoRI 3425 GAATTC G^AATTC 3811 GAATTC EcoT14I 1471 CCTTGG C^C[AT][AT]GG 3393 CCAAGG FbaI 1795 TGATCA T^GATCA 2799 TGATCA HapII 1381 CCGG C^CGG 3319 CCGG HindIII 2642 AAGCTT A^AGCTT 3492 AAGCTT HpaII 1381 CCGG C^CGG 3319 CCGG Ksp632I 641 GAAGAG CTCTTC(1/4) 758 GAAGAG MflI 163 AGATCC [GA]^GATC[CT] 3235 AGATCT MluNI 344 TGGCCA TGG^CCA 3538 TGGCCA MscI 344 TGGCCA TGG^CCA 3538 TGGCCA MspI 1381 CCGG C^CGG 3319 CCGG PflMI 1261 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2586 CCAAACTTTGG PvuII 131 CAGCTG CAG^CTG 1249 CAGCTG SspI 2275 AATATT AAT^ATT 2720 AATATT StyI 1471 CCTTGG C^C[AT][AT]GG 3393 CCAAGG Van91I 1261 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2586 CCAAACTTTGG XcmI 672 CCATATCTACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1596 CCACATATTTCATGG XhoII 163 AGATCC [GA]^GATC[CT] 3235 AGATCT XmnI 1618 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2444 GAACTCGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwNI ApoI AspHI AspI AvaII BbvI BciVI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DraI DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I