################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AccIII AclI AflII AflIII Alw44I ApaLI AscI AviII BbeI BbrPI BfrI BglI BglII BseAI BsiEI BsiWI BsmBI BspEI BspHI BspLU11I BssHII Bst1107I BstEII BstPI BstZ17I CpoI EagI EclXI Eco52I EcoO65I EcoT22I FseI FspI HindIII KasI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI PacI PaeR7I PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SbfI SfiI SfoI SgrAI SmaI SpeI SphI Sse8387I StuI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 881 GACGTC GACGT^C Acc65I 189 GGTACC G^GTACC AgeI 186 ACCGGT A^CCGGT AhdI 2805 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 1831 ATTAAT AT^TAAT AsnI 1831 ATTAAT AT^TAAT Asp718I 189 GGTACC G^GTACC AspEI 2805 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2744 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1034 CCCGAG C^[CT]CG[GA]G AvrII 854 CCTAGG C^CTAGG BamHI 2040 GGATCC G^GATCC BcgI 432 GCACAAGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1900 GGATAC GTATCC(6/5) BclI 3918 TGATCA T^GATCA BlnI 854 CCTAGG C^CTAGG BsaBI 517 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsoBI 1034 CCCGAG C^[CT]CG[GA]G BspDI 1838 ATCGAT AT^CGAT BssSI 3279 CACGAG CACGAG(-5/-1) BstAPI 3266 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1969 TTCGAA TT^CGAA ClaI 1838 ATCGAT AT^CGAT Eam1105I 2805 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 746 GAATTC G^AATTC EcoRV 830 GATATC GAT^ATC FbaI 3918 TGATCA T^GATCA KpnI 189 GGTACC GGTAC^C MamI 517 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NspV 1969 TTCGAA TT^CGAA PflFI 2744 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 186 ACCGGT A^CCGGT PshAI 2972 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1831 ATTAAT AT^TAAT SalI 1705 GTCGAC G^TCGAC ScaI 1766 AGTACT AGT^ACT SfuI 1969 TTCGAA TT^CGAA SwaI 4021 ATTTAAAT ATTT^AAAT Tth111I 2744 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2236 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 1705 GTCGAC GT^[AC][GT]AC 2951 GTAGAC AocI 295 CCTGAGG CC^T[ACGT]AGG 3550 CCTAAGG ApaI 733 GGGCCC GGGCC^C 2371 GGGCCC BalI 1908 TGGCCA TGG^CCA 2610 TGGCCA BanI 189 GGTACC G^G[CT][GA]CC 1344 GGCACC BlpI 1009 GCTAAGC GC^T[ACGT]AGC 2653 GCTGAGC Bpu1102I 1009 GCTAAGC GC^T[ACGT]AGC 2653 GCTGAGC BsaI 209 GAGACC GGTCTC(1/5) 482 GAGACC Bsp1407I 999 TGTACA T^GTACA 4070 TGTACA BsrFI 186 ACCGGT [GA]^CCGG[CT] 953 ACCGGC BsrGI 999 TGTACA T^GTACA 4070 TGTACA BstXI 1089 CCAACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1581 CCAATCTGATGG BstYI 1807 AGATCC [GA]^GATC[CT] 2040 GGATCC Bsu36I 295 CCTGAGG CC^T[ACGT]AGG 3550 CCTAAGG CelII 1009 GCTAAGC GC^T[ACGT]AGC 2653 GCTGAGC Cfr10I 186 ACCGGT [GA]^CCGG[CT] 953 ACCGGC DrdI 881 GACGTCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2858 GACATTGGCGTC Eco81I 295 CCTGAGG CC^T[ACGT]AGG 3550 CCTAAGG EcoNI 2994 CCTTTGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3857 CCTCTTTTAGG KspI 113 CCGCGG CCGC^GG 3255 CCGCGG MflI 1807 AGATCC [GA]^GATC[CT] 2040 GGATCC MluNI 1908 TGGCCA TGG^CCA 2610 TGGCCA MscI 1908 TGGCCA TGG^CCA 2610 TGGCCA MslI 2983 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3149 CATCTGAATG PflMI 773 CCAAGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3208 CCACATCCTGG PstI 1349 CTGCAG CTGCA^G 2845 CTGCAG SacII 113 CCGCGG CCGC^GG 3255 CCGCGG SexAI 192 ACCTGGT A^CC[AT]GGT 1494 ACCTGGT SfcI 1349 CTGCAG C^T[GA][CT]AG 2845 CTGCAG SnaBI 3396 TACGTA TAC^GTA 3889 TACGTA SspBI 999 TGTACA T^GTACA 4070 TGTACA TfiI 649 GAATC G^A[AT]TC 3612 GATTC Van91I 773 CCAAGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3208 CCACATCCTGG XcmI 1578 CCACCAATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2022 CCATCTGTGCCCTGG XhoII 1807 AGATCC [GA]^GATC[CT] 2040 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI Aor51HI ApoI Asp700I AspHI AvaII BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco47III EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NheI NlaIII NlaIV PleI PpuMI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SmlI SspI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI