##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AccIII AclI AflII AflIII Alw44I
ApaLI AscI AviII BbeI BbrPI
BfrI BglI BglII BseAI BsiEI
BsiWI BsmBI BspEI BspHI BspLU11I
BssHII Bst1107I BstEII BstPI BstZ17I
CpoI EagI EclXI Eco52I EcoO65I
EcoT22I FseI FspI HindIII KasI
MfeI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NsiI NspI PacI PaeR7I
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SbfI SfiI SfoI
SgrAI SmaI SpeI SphI Sse8387I
StuI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 881 GACGTC GACGT^C
Acc65I 189 GGTACC G^GTACC
AgeI 186 ACCGGT A^CCGGT
AhdI 2805 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 1831 ATTAAT AT^TAAT
AsnI 1831 ATTAAT AT^TAAT
Asp718I 189 GGTACC G^GTACC
AspEI 2805 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2744 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1034 CCCGAG C^[CT]CG[GA]G
AvrII 854 CCTAGG C^CTAGG
BamHI 2040 GGATCC G^GATCC
BcgI 432 GCACAAGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1900 GGATAC GTATCC(6/5)
BclI 3918 TGATCA T^GATCA
BlnI 854 CCTAGG C^CTAGG
BsaBI 517 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 1034 CCCGAG C^[CT]CG[GA]G
BspDI 1838 ATCGAT AT^CGAT
BssSI 3279 CACGAG CACGAG(-5/-1)
BstAPI 3266 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1969 TTCGAA TT^CGAA
ClaI 1838 ATCGAT AT^CGAT
Eam1105I 2805 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 746 GAATTC G^AATTC
EcoRV 830 GATATC GAT^ATC
FbaI 3918 TGATCA T^GATCA
KpnI 189 GGTACC GGTAC^C
MamI 517 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspV 1969 TTCGAA TT^CGAA
PflFI 2744 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 186 ACCGGT A^CCGGT
PshAI 2972 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1831 ATTAAT AT^TAAT
SalI 1705 GTCGAC G^TCGAC
ScaI 1766 AGTACT AGT^ACT
SfuI 1969 TTCGAA TT^CGAA
SwaI 4021 ATTTAAAT ATTT^AAAT
Tth111I 2744 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2236 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 1705 GTCGAC GT^[AC][GT]AC
2951 GTAGAC
AocI 295 CCTGAGG CC^T[ACGT]AGG
3550 CCTAAGG
ApaI 733 GGGCCC GGGCC^C
2371 GGGCCC
BalI 1908 TGGCCA TGG^CCA
2610 TGGCCA
BanI 189 GGTACC G^G[CT][GA]CC
1344 GGCACC
BlpI 1009 GCTAAGC GC^T[ACGT]AGC
2653 GCTGAGC
Bpu1102I 1009 GCTAAGC GC^T[ACGT]AGC
2653 GCTGAGC
BsaI 209 GAGACC GGTCTC(1/5)
482 GAGACC
Bsp1407I 999 TGTACA T^GTACA
4070 TGTACA
BsrFI 186 ACCGGT [GA]^CCGG[CT]
953 ACCGGC
BsrGI 999 TGTACA T^GTACA
4070 TGTACA
BstXI 1089 CCAACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1581 CCAATCTGATGG
BstYI 1807 AGATCC [GA]^GATC[CT]
2040 GGATCC
Bsu36I 295 CCTGAGG CC^T[ACGT]AGG
3550 CCTAAGG
CelII 1009 GCTAAGC GC^T[ACGT]AGC
2653 GCTGAGC
Cfr10I 186 ACCGGT [GA]^CCGG[CT]
953 ACCGGC
DrdI 881 GACGTCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2858 GACATTGGCGTC
Eco81I 295 CCTGAGG CC^T[ACGT]AGG
3550 CCTAAGG
EcoNI 2994 CCTTTGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3857 CCTCTTTTAGG
KspI 113 CCGCGG CCGC^GG
3255 CCGCGG
MflI 1807 AGATCC [GA]^GATC[CT]
2040 GGATCC
MluNI 1908 TGGCCA TGG^CCA
2610 TGGCCA
MscI 1908 TGGCCA TGG^CCA
2610 TGGCCA
MslI 2983 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3149 CATCTGAATG
PflMI 773 CCAAGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3208 CCACATCCTGG
PstI 1349 CTGCAG CTGCA^G
2845 CTGCAG
SacII 113 CCGCGG CCGC^GG
3255 CCGCGG
SexAI 192 ACCTGGT A^CC[AT]GGT
1494 ACCTGGT
SfcI 1349 CTGCAG C^T[GA][CT]AG
2845 CTGCAG
SnaBI 3396 TACGTA TAC^GTA
3889 TACGTA
SspBI 999 TGTACA T^GTACA
4070 TGTACA
TfiI 649 GAATC G^A[AT]TC
3612 GATTC
Van91I 773 CCAAGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3208 CCACATCCTGG
XcmI 1578 CCACCAATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2022 CCATCTGTGCCCTGG
XhoII 1807 AGATCC [GA]^GATC[CT]
2040 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI Aor51HI ApoI
Asp700I AspHI AvaII BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI Eco47III
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NheI NlaIII NlaIV PleI PpuMI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SmlI SspI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI