##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AgeI AhdI AocI Aor51HI ApaI
AscI Asp718I AspEI AspI AviII
BalI BamHI BbeI BbvCI BcgI
BcnI BglI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BspEI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
FseI FspI HaeII HapII Hin1I
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NotI NruI NspV PflFI PinAI
PmeI PshAI PstI PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SpeI Sse8387I
StuI SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 277 CGCG CG^CG
AclI 2754 AACGTT AA^CGTT
Alw44I 2540 GTGCAC G^TGCAC
AlwNI 2068 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2540 GTGCAC G^TGCAC
AvaI 2397 CTCGAG C^[CT]CG[GA]G
AvrII 926 CCTAGG C^CTAGG
BanI 1294 GGCACC G^G[CT][GA]CC
BanII 2574 GAGCTC G[GA]GC[CT]^C
BciVI 194 GGATAC GTATCC(6/5)
BclI 2106 TGATCA T^GATCA
BlnI 926 CCTAGG C^CTAGG
BsaI 1444 GAGACC GGTCTC(1/5)
BsgI 2053 GTGCAG GTGCAG(16/14)
BsoBI 2397 CTCGAG C^[CT]CG[GA]G
BspDI 1748 ATCGAT AT^CGAT
BspHI 1727 TCATGA T^CATGA
BspLU11I 1710 ACATGT A^CATGT
BsrBI 3237 GAGCGG CCGCTC(-3/-3)
BstAPI 715 GCACCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 277 CGCG CG^CG
CfoI 276 GCGC GCG^C
ClaI 1748 ATCGAT AT^CGAT
DraIII 760 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 1614 GATATC GAT^ATC
EcoT14I 926 CCTAGG C^C[AT][AT]GG
EcoT22I 1924 ATGCAT ATGCA^T
FbaI 2106 TGATCA T^GATCA
HgaI 2556 GACGC GACGC(5/10)
HhaI 276 GCGC GCG^C
HinP1I 276 GCGC G^CGC
MvnI 277 CGCG CG^CG
NheI 1142 GCTAGC G^CTAGC
NsiI 1924 ATGCAT ATGCA^T
PaeR7I 2397 CTCGAG C^TCGAG
Psp1406I 2754 AACGTT AA^CGTT
RcaI 1727 TCATGA T^CATGA
SacI 2574 GAGCTC GAGCT^C
SapI 724 GCTCTTC GCTCTTC(1/4)
ScaI 4042 AGTACT AGT^ACT
SexAI 3601 ACCTGGT A^CC[AT]GGT
SphI 1458 GCATGC GCATG^C
StyI 926 CCTAGG C^C[AT][AT]GG
XbaI 1610 TCTAGA T^CTAGA
XhoI 2397 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 208 ACGTGT A^C[GA][CT]GT
1710 ACATGT
Asp700I 3154 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3435 GAATGATTTC
BbrPI 207 CACGTG CAC^GTG
2123 CACGTG
BglII 789 AGATCT A^GATCT
3554 AGATCT
BsmI 48 GAATGC GAATGC(1/-1)
3577 GAATGC
Bsp1407I 251 TGTACA T^GTACA
1713 TGTACA
BsrDI 616 CATTGC GCAATG(2/0)
2581 GCAATG
BsrGI 251 TGTACA T^GTACA
1713 TGTACA
BstXI 3595 CCACCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3598 CCAACCTGGTGG
DsaI 1263 CCGTGG C^C[GA][CT]GG
1305 CCGTGG
EaeI 1260 TGGCCG [CT]^GGCC[GA]
2254 TGGCCG
EcoRI 1521 GAATTC G^AATTC
1530 GAATTC
FauI 246 CCCGC CCCGC(4/6)
1508 CCCGC
HincII 582 GTTGAC GT[CT]^[GA]AC
2757 GTTGAC
HindII 582 GTTGAC GT[CT]^[GA]AC
2757 GTTGAC
PacI 1659 TTAATTAA TTAAT^TAA
2895 TTAATTAA
PmaCI 207 CACGTG CAC^GTG
2123 CACGTG
PmlI 207 CACGTG CAC^GTG
2123 CACGTG
PpuMI 734 AGGTCCT [GA]G^G[AT]CC[CT]
3682 AGGTCCT
SnaBI 974 TACGTA TAC^GTA
3033 TACGTA
SspBI 251 TGTACA T^GTACA
1713 TGTACA
XmnI 3154 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3435 GAATGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI ApoI AseI AsnI AspHI
AvaII BbsI BbvI BfaI BfrI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PshBI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII