################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII Acc65I AccI AccIII AcyI AgeI AhdI AocI Aor51HI ApaI AscI Asp718I AspEI AspI AviII BalI BamHI BbeI BbvCI BcgI BcnI BglI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspEI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I FseI FspI HaeII HapII Hin1I HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NspV PflFI PinAI PmeI PshAI PstI PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SpeI Sse8387I StuI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccII 277 CGCG CG^CG AclI 2754 AACGTT AA^CGTT Alw44I 2540 GTGCAC G^TGCAC AlwNI 2068 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 2540 GTGCAC G^TGCAC AvaI 2397 CTCGAG C^[CT]CG[GA]G AvrII 926 CCTAGG C^CTAGG BanI 1294 GGCACC G^G[CT][GA]CC BanII 2574 GAGCTC G[GA]GC[CT]^C BciVI 194 GGATAC GTATCC(6/5) BclI 2106 TGATCA T^GATCA BlnI 926 CCTAGG C^CTAGG BsaI 1444 GAGACC GGTCTC(1/5) BsgI 2053 GTGCAG GTGCAG(16/14) BsoBI 2397 CTCGAG C^[CT]CG[GA]G BspDI 1748 ATCGAT AT^CGAT BspHI 1727 TCATGA T^CATGA BspLU11I 1710 ACATGT A^CATGT BsrBI 3237 GAGCGG CCGCTC(-3/-3) BstAPI 715 GCACCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 277 CGCG CG^CG CfoI 276 GCGC GCG^C ClaI 1748 ATCGAT AT^CGAT DraIII 760 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRV 1614 GATATC GAT^ATC EcoT14I 926 CCTAGG C^C[AT][AT]GG EcoT22I 1924 ATGCAT ATGCA^T FbaI 2106 TGATCA T^GATCA HgaI 2556 GACGC GACGC(5/10) HhaI 276 GCGC GCG^C HinP1I 276 GCGC G^CGC MvnI 277 CGCG CG^CG NheI 1142 GCTAGC G^CTAGC NsiI 1924 ATGCAT ATGCA^T PaeR7I 2397 CTCGAG C^TCGAG Psp1406I 2754 AACGTT AA^CGTT RcaI 1727 TCATGA T^CATGA SacI 2574 GAGCTC GAGCT^C SapI 724 GCTCTTC GCTCTTC(1/4) ScaI 4042 AGTACT AGT^ACT SexAI 3601 ACCTGGT A^CC[AT]GGT SphI 1458 GCATGC GCATG^C StyI 926 CCTAGG C^C[AT][AT]GG XbaI 1610 TCTAGA T^CTAGA XhoI 2397 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflIII 208 ACGTGT A^C[GA][CT]GT 1710 ACATGT Asp700I 3154 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3435 GAATGATTTC BbrPI 207 CACGTG CAC^GTG 2123 CACGTG BglII 789 AGATCT A^GATCT 3554 AGATCT BsmI 48 GAATGC GAATGC(1/-1) 3577 GAATGC Bsp1407I 251 TGTACA T^GTACA 1713 TGTACA BsrDI 616 CATTGC GCAATG(2/0) 2581 GCAATG BsrGI 251 TGTACA T^GTACA 1713 TGTACA BstXI 3595 CCACCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3598 CCAACCTGGTGG DsaI 1263 CCGTGG C^C[GA][CT]GG 1305 CCGTGG EaeI 1260 TGGCCG [CT]^GGCC[GA] 2254 TGGCCG EcoRI 1521 GAATTC G^AATTC 1530 GAATTC FauI 246 CCCGC CCCGC(4/6) 1508 CCCGC HincII 582 GTTGAC GT[CT]^[GA]AC 2757 GTTGAC HindII 582 GTTGAC GT[CT]^[GA]AC 2757 GTTGAC PacI 1659 TTAATTAA TTAAT^TAA 2895 TTAATTAA PmaCI 207 CACGTG CAC^GTG 2123 CACGTG PmlI 207 CACGTG CAC^GTG 2123 CACGTG PpuMI 734 AGGTCCT [GA]G^G[AT]CC[CT] 3682 AGGTCCT SnaBI 974 TACGTA TAC^GTA 3033 TACGTA SspBI 251 TGTACA T^GTACA 1713 TGTACA XmnI 3154 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3435 GAATGATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AciI AcsI AfaI AflII AluI AlwI ApoI AseI AsnI AspHI AvaII BbsI BbvI BfaI BfrI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PshBI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII