##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
BbrPI BcgI BfrI BsaBI BsiEI
BsiWI BsmI Bsp1407I BsrBI BsrGI
BssSI Bst1107I BstZ17I CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FseI FspI KpnI
KspI MamI MluI NaeI NgoMIV
NotI NruI NsiI PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SexAI SfiI SmaI SnaBI
SpeI SspBI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AatII 4465 GACGTC GACGT^C
AspI 1808 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3331 CCTAGG C^CTAGG
BciVI 585 GGATAC GTATCC(6/5)
BglII 2467 AGATCT A^GATCT
BlnI 3331 CCTAGG C^CTAGG
BsaAI 4560 TACGTG [CT]AC^GT[GA]
BspDI 3260 ATCGAT AT^CGAT
BspLU11I 5037 ACATGT A^CATGT
BssHII 30 GCGCGC G^CGCGC
BstBI 4326 TTCGAA TT^CGAA
BstEII 1303 GGTCACC G^GT[ACGT]ACC
BstPI 1303 GGTCACC G^GT[ACGT]ACC
ClaI 3260 ATCGAT AT^CGAT
DrdI 182 GACCTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1303 GGTCACC G^GT[ACGT]ACC
EcoRI 3035 GAATTC G^AATTC
HgaI 914 GACGC GACGC(5/10)
HpaI 2164 GTTAAC GTT^AAC
MfeI 2015 CAATTG C^AATTG
MslI 3602 CATATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2015 CAATTG C^AATTG
NheI 2189 GCTAGC G^CTAGC
NspV 4326 TTCGAA TT^CGAA
PaeR7I 4517 CTCGAG C^TCGAG
PflFI 1808 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfuI 4326 TTCGAA TT^CGAA
SgrAI 74 CACCGGCG C[GA]^CCGG[CT]G
SspI 2339 AATATT AAT^ATT
Tth111I 1808 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4517 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 333 TCCGGA T^CCGGA
4377 TCCGGA
AflIII 855 ACGTGT A^C[GA][CT]GT
5037 ACATGT
ApaI 2358 GGGCCC GGGCC^C
3570 GGGCCC
AvaI 106 CTCGGG C^[CT]CG[GA]G
4517 CTCGAG
BamHI 957 GGATCC G^GATCC
1144 GGATCC
BbeI 78 GGCGCC GGCGC^C
125 GGCGCC
BclI 3945 TGATCA T^GATCA
5458 TGATCA
BseAI 333 TCCGGA T^CCGGA
4377 TCCGGA
BsmBI 912 GAGACG CGTCTC(1/5)
2685 GAGACG
BsoBI 106 CTCGGG C^[CT]CG[GA]G
4517 CTCGAG
BspEI 333 TCCGGA T^CCGGA
4377 TCCGGA
BspHI 2350 TCATGA T^CATGA
5564 TCATGA
BsrFI 75 ACCGGC [GA]^CCGG[CT]
3103 GCCGGT
BstAPI 2480 GCACTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5010 GCATGCCCTGC
Cfr10I 75 ACCGGC [GA]^CCGG[CT]
3103 GCCGGT
DraIII 2481 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
4023 CACAGTGTG
FbaI 3945 TGATCA T^GATCA
5458 TGATCA
KasI 78 GGCGCC G^GCGCC
125 GGCGCC
MroI 333 TCCGGA T^CCGGA
4377 TCCGGA
NarI 78 GGCGCC GG^CGCC
125 GGCGCC
NdeI 2506 CATATG CA^TATG
5063 CATATG
RcaI 2350 TCATGA T^CATGA
5564 TCATGA
SbfI 1822 CCTGCAGG CCTGCA^GG
1906 CCTGCAGG
SfoI 78 GGCGCC GGC^GCC
125 GGCGCC
SphI 3305 GCATGC GCATG^C
5010 GCATGC
Sse8387I 1822 CCTGCAGG CCTGCA^GG
1906 CCTGCAGG
StuI 636 AGGCCT AGG^CCT
5516 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII XmnI