##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AviII       
BbrPI       BcgI        BfrI        BsaBI       BsiEI       
BsiWI       BsmI        Bsp1407I    BsrBI       BsrGI       
BssSI       Bst1107I    BstZ17I     CpoI        DraI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoRV       EcoT22I     FseI        FspI        KpnI        
KspI        MamI        MluI        NaeI        NgoMIV      
NotI        NruI        NsiI        PacI        PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
ScaI        SexAI       SfiI        SmaI        SnaBI       
SpeI        SspBI       SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AatII        4465  GACGTC               GACGT^C
AspI         1808  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        3331  CCTAGG               C^CTAGG
BciVI         585  GGATAC               GTATCC(6/5)
BglII        2467  AGATCT               A^GATCT
BlnI         3331  CCTAGG               C^CTAGG
BsaAI        4560  TACGTG               [CT]AC^GT[GA]
BspDI        3260  ATCGAT               AT^CGAT
BspLU11I     5037  ACATGT               A^CATGT
BssHII         30  GCGCGC               G^CGCGC
BstBI        4326  TTCGAA               TT^CGAA
BstEII       1303  GGTCACC              G^GT[ACGT]ACC
BstPI        1303  GGTCACC              G^GT[ACGT]ACC
ClaI         3260  ATCGAT               AT^CGAT
DrdI          182  GACCTGGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      1303  GGTCACC              G^GT[ACGT]ACC
EcoRI        3035  GAATTC               G^AATTC
HgaI          914  GACGC                GACGC(5/10)
HpaI         2164  GTTAAC               GTT^AAC
MfeI         2015  CAATTG               C^AATTG
MslI         3602  CATATTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         2015  CAATTG               C^AATTG
NheI         2189  GCTAGC               G^CTAGC
NspV         4326  TTCGAA               TT^CGAA
PaeR7I       4517  CTCGAG               C^TCGAG
PflFI        1808  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SfuI         4326  TTCGAA               TT^CGAA
SgrAI          74  CACCGGCG             C[GA]^CCGG[CT]G
SspI         2339  AATATT               AAT^ATT
Tth111I      1808  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         4517  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccIII        333  TCCGGA               T^CCGGA
             4377  TCCGGA              
AflIII        855  ACGTGT               A^C[GA][CT]GT
             5037  ACATGT              
ApaI         2358  GGGCCC               GGGCC^C
             3570  GGGCCC              
AvaI          106  CTCGGG               C^[CT]CG[GA]G
             4517  CTCGAG              
BamHI         957  GGATCC               G^GATCC
             1144  GGATCC              
BbeI           78  GGCGCC               GGCGC^C
              125  GGCGCC              
BclI         3945  TGATCA               T^GATCA
             5458  TGATCA              
BseAI         333  TCCGGA               T^CCGGA
             4377  TCCGGA              
BsmBI         912  GAGACG               CGTCTC(1/5)
             2685  GAGACG              
BsoBI         106  CTCGGG               C^[CT]CG[GA]G
             4517  CTCGAG              
BspEI         333  TCCGGA               T^CCGGA
             4377  TCCGGA              
BspHI        2350  TCATGA               T^CATGA
             5564  TCATGA              
BsrFI          75  ACCGGC               [GA]^CCGG[CT]
             3103  GCCGGT              
BstAPI       2480  GCACTCGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             5010  GCATGCCCTGC         
Cfr10I         75  ACCGGC               [GA]^CCGG[CT]
             3103  GCCGGT              
DraIII       2481  CACTCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4023  CACAGTGTG           
FbaI         3945  TGATCA               T^GATCA
             5458  TGATCA              
KasI           78  GGCGCC               G^GCGCC
              125  GGCGCC              
MroI          333  TCCGGA               T^CCGGA
             4377  TCCGGA              
NarI           78  GGCGCC               GG^CGCC
              125  GGCGCC              
NdeI         2506  CATATG               CA^TATG
             5063  CATATG              
RcaI         2350  TCATGA               T^CATGA
             5564  TCATGA              
SbfI         1822  CCTGCAGG             CCTGCA^GG
             1906  CCTGCAGG            
SfoI           78  GGCGCC               GGC^GCC
              125  GGCGCC              
SphI         3305  GCATGC               GCATG^C
             5010  GCATGC              
Sse8387I     1822  CCTGCAGG             CCTGCA^GG
             1906  CCTGCAGG            
StuI          636  AGGCCT               AGG^CCT
             5516  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  130 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        ApoI        
Asp700I     AspHI       AvaII       BalI        BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XbaI        XcmI        XhoII       XmnI