################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## Acc65I AccI AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AviII BbrPI BcgI BfrI BsaBI BsiEI BsiWI BsmI Bsp1407I BsrBI BsrGI BssSI Bst1107I BstZ17I CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI KpnI KspI MamI MluI NaeI NgoMIV NotI NruI NsiI PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfiI SmaI SnaBI SpeI SspBI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AatII 4465 GACGTC GACGT^C AspI 1808 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3331 CCTAGG C^CTAGG BciVI 585 GGATAC GTATCC(6/5) BglII 2467 AGATCT A^GATCT BlnI 3331 CCTAGG C^CTAGG BsaAI 4560 TACGTG [CT]AC^GT[GA] BspDI 3260 ATCGAT AT^CGAT BspLU11I 5037 ACATGT A^CATGT BssHII 30 GCGCGC G^CGCGC BstBI 4326 TTCGAA TT^CGAA BstEII 1303 GGTCACC G^GT[ACGT]ACC BstPI 1303 GGTCACC G^GT[ACGT]ACC ClaI 3260 ATCGAT AT^CGAT DrdI 182 GACCTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1303 GGTCACC G^GT[ACGT]ACC EcoRI 3035 GAATTC G^AATTC HgaI 914 GACGC GACGC(5/10) HpaI 2164 GTTAAC GTT^AAC MfeI 2015 CAATTG C^AATTG MslI 3602 CATATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 2015 CAATTG C^AATTG NheI 2189 GCTAGC G^CTAGC NspV 4326 TTCGAA TT^CGAA PaeR7I 4517 CTCGAG C^TCGAG PflFI 1808 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC SfuI 4326 TTCGAA TT^CGAA SgrAI 74 CACCGGCG C[GA]^CCGG[CT]G SspI 2339 AATATT AAT^ATT Tth111I 1808 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 4517 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccIII 333 TCCGGA T^CCGGA 4377 TCCGGA AflIII 855 ACGTGT A^C[GA][CT]GT 5037 ACATGT ApaI 2358 GGGCCC GGGCC^C 3570 GGGCCC AvaI 106 CTCGGG C^[CT]CG[GA]G 4517 CTCGAG BamHI 957 GGATCC G^GATCC 1144 GGATCC BbeI 78 GGCGCC GGCGC^C 125 GGCGCC BclI 3945 TGATCA T^GATCA 5458 TGATCA BseAI 333 TCCGGA T^CCGGA 4377 TCCGGA BsmBI 912 GAGACG CGTCTC(1/5) 2685 GAGACG BsoBI 106 CTCGGG C^[CT]CG[GA]G 4517 CTCGAG BspEI 333 TCCGGA T^CCGGA 4377 TCCGGA BspHI 2350 TCATGA T^CATGA 5564 TCATGA BsrFI 75 ACCGGC [GA]^CCGG[CT] 3103 GCCGGT BstAPI 2480 GCACTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5010 GCATGCCCTGC Cfr10I 75 ACCGGC [GA]^CCGG[CT] 3103 GCCGGT DraIII 2481 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG 4023 CACAGTGTG FbaI 3945 TGATCA T^GATCA 5458 TGATCA KasI 78 GGCGCC G^GCGCC 125 GGCGCC MroI 333 TCCGGA T^CCGGA 4377 TCCGGA NarI 78 GGCGCC GG^CGCC 125 GGCGCC NdeI 2506 CATATG CA^TATG 5063 CATATG RcaI 2350 TCATGA T^CATGA 5564 TCATGA SbfI 1822 CCTGCAGG CCTGCA^GG 1906 CCTGCAGG SfoI 78 GGCGCC GGC^GCC 125 GGCGCC SphI 3305 GCATGC GCATG^C 5010 GCATGC Sse8387I 1822 CCTGCAGG CCTGCA^GG 1906 CCTGCAGG StuI 636 AGGCCT AGG^CCT 5516 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII XmnI