##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AclI AcyI AflII AhdI
ApaI AscI AspEI BbeI BclI
BfrI BglII BsaBI BsaHI BsiEI
BsiWI BspDI BssHII ClaI CpoI
EagI Eam1105I EclXI Eco52I FbaI
FseI Hin1I HincII HindII HpaI
KasI MamI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PmeI Psp1406I
PvuI RsrII SalI SbfI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 3704 GGTACC G^GTACC
AccIII 2337 TCCGGA T^CCGGA
AflIII 1863 ACATGT A^C[GA][CT]GT
AgeI 4756 ACCGGT A^CCGGT
AseI 4437 ATTAAT AT^TAAT
AsnI 4437 ATTAAT AT^TAAT
Asp718I 3704 GGTACC G^GTACC
AvrII 1900 CCTAGG C^CTAGG
BbrPI 1085 CACGTG CAC^GTG
BlnI 1900 CCTAGG C^CTAGG
BseAI 2337 TCCGGA T^CCGGA
BsmBI 4706 GAGACG CGTCTC(1/5)
Bsp1407I 4592 TGTACA T^GTACA
BspEI 2337 TCCGGA T^CCGGA
BspLU11I 1863 ACATGT A^CATGT
BsrBI 1488 CCGCTC CCGCTC(-3/-3)
BsrDI 1984 CATTGC GCAATG(2/0)
BsrGI 4592 TGTACA T^GTACA
BssSI 2295 CTCGTG CACGAG(-5/-1)
Bst1107I 2661 GTATAC GTA^TAC
BstBI 1527 TTCGAA TT^CGAA
BstZ17I 2661 GTATAC GTA^TAC
DrdI 3980 GACTTCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 153 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4586 GATATC GAT^ATC
EcoT22I 284 ATGCAT ATGCA^T
HgaI 714 GCGTC GACGC(5/10)
HindIII 3923 AAGCTT A^AGCTT
KpnI 3704 GGTACC GGTAC^C
KspI 37 CCGCGG CCGC^GG
MroI 2337 TCCGGA T^CCGGA
NsiI 284 ATGCAT ATGCA^T
NspV 1527 TTCGAA TT^CGAA
PaeR7I 4833 CTCGAG C^TCGAG
PinAI 4756 ACCGGT A^CCGGT
PmaCI 1085 CACGTG CAC^GTG
PmlI 1085 CACGTG CAC^GTG
PshAI 205 GACTCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4437 ATTAAT AT^TAAT
SacII 37 CCGCGG CCGC^GG
ScaI 3843 AGTACT AGT^ACT
SexAI 3639 ACCTGGT A^CC[AT]GGT
SfuI 1527 TTCGAA TT^CGAA
SspBI 4592 TGTACA T^GTACA
SspI 1722 AATATT AAT^ATT
XhoI 4833 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 38 CGCG CG^CG
85 CGCG
Alw44I 398 GTGCAC G^TGCAC
1082 GTGCAC
AocI 2348 CCTGAGG CC^T[ACGT]AGG
2642 CCTGAGG
Aor51HI 552 AGCGCT AGC^GCT
2015 AGCGCT
ApaLI 398 GTGCAC G^TGCAC
1082 GTGCAC
AspI 299 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4025 GACACAGTC
AviII 1050 TGCGCA TGC^GCA
1584 TGCGCA
BamHI 253 GGATCC G^GATCC
1090 GGATCC
BbvCI 2355 CCTCAGC CCTCAGC(-5/-2)
2984 GCTGAGG
BsaAI 764 CACGTA [CT]AC^GT[GA]
1085 CACGTG
BsmI 4294 GAATGC GAATGC(1/-1)
286 GCATTC
BspHI 234 TCATGA T^CATGA
837 TCATGA
BsrFI 1010 ACCGGC [GA]^CCGG[CT]
4756 ACCGGT
BstAPI 2511 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2574 GCAGTCTTTGC
BstEII 1711 GGTAACC G^GT[ACGT]ACC
1904 GGTCACC
BstPI 1711 GGTAACC G^GT[ACGT]ACC
1904 GGTCACC
BstUI 38 CGCG CG^CG
85 CGCG
BstXI 871 CCACACATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1176 CCACAGGCCTGG
Bsu36I 2348 CCTGAGG CC^T[ACGT]AGG
2642 CCTGAGG
Cfr10I 1010 ACCGGC [GA]^CCGG[CT]
4756 ACCGGT
DraI 911 TTTAAA TTT^AAA
4852 TTTAAA
DraIII 431 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
4211 CACAAGGTG
Eco47III 552 AGCGCT AGC^GCT
2015 AGCGCT
Eco81I 2348 CCTGAGG CC^T[ACGT]AGG
2642 CCTGAGG
EcoO65I 1711 GGTAACC G^GT[ACGT]ACC
1904 GGTCACC
EcoRI 77 GAATTC G^AATTC
2889 GAATTC
FspI 1050 TGCGCA TGC^GCA
1584 TGCGCA
MvnI 38 CGCG CG^CG
85 CGCG
NcoI 103 CCATGG C^CATGG
511 CCATGG
PflFI 299 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4025 GACACAGTC
PflMI 601 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
763 CCACGTACTGG
PpuMI 1222 GGGACCT [GA]G^G[AT]CC[CT]
3636 GGGACCT
RcaI 234 TCATGA T^CATGA
837 TCATGA
SapI 3031 GAAGAGC GCTCTTC(1/4)
377 GCTCTTC
Tth111I 299 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4025 GACACAGTC
Van91I 601 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
763 CCACGTACTGG
XbaI 4093 TCTAGA T^CTAGA
4605 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcgI BciVI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI