################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII AclI AcyI AflII AhdI ApaI AscI AspEI BbeI BclI BfrI BglII BsaBI BsaHI BsiEI BsiWI BspDI BssHII ClaI CpoI EagI Eam1105I EclXI Eco52I FbaI FseI Hin1I HincII HindII HpaI KasI MamI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PmeI Psp1406I PvuI RsrII SalI SbfI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 3704 GGTACC G^GTACC AccIII 2337 TCCGGA T^CCGGA AflIII 1863 ACATGT A^C[GA][CT]GT AgeI 4756 ACCGGT A^CCGGT AseI 4437 ATTAAT AT^TAAT AsnI 4437 ATTAAT AT^TAAT Asp718I 3704 GGTACC G^GTACC AvrII 1900 CCTAGG C^CTAGG BbrPI 1085 CACGTG CAC^GTG BlnI 1900 CCTAGG C^CTAGG BseAI 2337 TCCGGA T^CCGGA BsmBI 4706 GAGACG CGTCTC(1/5) Bsp1407I 4592 TGTACA T^GTACA BspEI 2337 TCCGGA T^CCGGA BspLU11I 1863 ACATGT A^CATGT BsrBI 1488 CCGCTC CCGCTC(-3/-3) BsrDI 1984 CATTGC GCAATG(2/0) BsrGI 4592 TGTACA T^GTACA BssSI 2295 CTCGTG CACGAG(-5/-1) Bst1107I 2661 GTATAC GTA^TAC BstBI 1527 TTCGAA TT^CGAA BstZ17I 2661 GTATAC GTA^TAC DrdI 3980 GACTTCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 153 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 4586 GATATC GAT^ATC EcoT22I 284 ATGCAT ATGCA^T HgaI 714 GCGTC GACGC(5/10) HindIII 3923 AAGCTT A^AGCTT KpnI 3704 GGTACC GGTAC^C KspI 37 CCGCGG CCGC^GG MroI 2337 TCCGGA T^CCGGA NsiI 284 ATGCAT ATGCA^T NspV 1527 TTCGAA TT^CGAA PaeR7I 4833 CTCGAG C^TCGAG PinAI 4756 ACCGGT A^CCGGT PmaCI 1085 CACGTG CAC^GTG PmlI 1085 CACGTG CAC^GTG PshAI 205 GACTCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4437 ATTAAT AT^TAAT SacII 37 CCGCGG CCGC^GG ScaI 3843 AGTACT AGT^ACT SexAI 3639 ACCTGGT A^CC[AT]GGT SfuI 1527 TTCGAA TT^CGAA SspBI 4592 TGTACA T^GTACA SspI 1722 AATATT AAT^ATT XhoI 4833 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccII 38 CGCG CG^CG 85 CGCG Alw44I 398 GTGCAC G^TGCAC 1082 GTGCAC AocI 2348 CCTGAGG CC^T[ACGT]AGG 2642 CCTGAGG Aor51HI 552 AGCGCT AGC^GCT 2015 AGCGCT ApaLI 398 GTGCAC G^TGCAC 1082 GTGCAC AspI 299 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4025 GACACAGTC AviII 1050 TGCGCA TGC^GCA 1584 TGCGCA BamHI 253 GGATCC G^GATCC 1090 GGATCC BbvCI 2355 CCTCAGC CCTCAGC(-5/-2) 2984 GCTGAGG BsaAI 764 CACGTA [CT]AC^GT[GA] 1085 CACGTG BsmI 4294 GAATGC GAATGC(1/-1) 286 GCATTC BspHI 234 TCATGA T^CATGA 837 TCATGA BsrFI 1010 ACCGGC [GA]^CCGG[CT] 4756 ACCGGT BstAPI 2511 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2574 GCAGTCTTTGC BstEII 1711 GGTAACC G^GT[ACGT]ACC 1904 GGTCACC BstPI 1711 GGTAACC G^GT[ACGT]ACC 1904 GGTCACC BstUI 38 CGCG CG^CG 85 CGCG BstXI 871 CCACACATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1176 CCACAGGCCTGG Bsu36I 2348 CCTGAGG CC^T[ACGT]AGG 2642 CCTGAGG Cfr10I 1010 ACCGGC [GA]^CCGG[CT] 4756 ACCGGT DraI 911 TTTAAA TTT^AAA 4852 TTTAAA DraIII 431 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG 4211 CACAAGGTG Eco47III 552 AGCGCT AGC^GCT 2015 AGCGCT Eco81I 2348 CCTGAGG CC^T[ACGT]AGG 2642 CCTGAGG EcoO65I 1711 GGTAACC G^GT[ACGT]ACC 1904 GGTCACC EcoRI 77 GAATTC G^AATTC 2889 GAATTC FspI 1050 TGCGCA TGC^GCA 1584 TGCGCA MvnI 38 CGCG CG^CG 85 CGCG NcoI 103 CCATGG C^CATGG 511 CCATGG PflFI 299 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4025 GACACAGTC PflMI 601 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 763 CCACGTACTGG PpuMI 1222 GGGACCT [GA]G^G[AT]CC[CT] 3636 GGGACCT RcaI 234 TCATGA T^CATGA 837 TCATGA SapI 3031 GAAGAGC GCTCTTC(1/4) 377 GCTCTTC Tth111I 299 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4025 GACACAGTC Van91I 601 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 763 CCACGTACTGG XbaI 4093 TCTAGA T^CTAGA 4605 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcgI BciVI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI