##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccIII AclI AflII AhdI
AocI Aor51HI ApaI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BfrI BlnI BsaAI BsaBI
BseAI BsiWI BspEI BsrDI BssHII
BssSI Bst1107I BstXI BstZ17I Bsu36I
CpoI DraIII Eam1105I Eco47III Eco81I
EcoNI EcoRV FseI FspI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NgoMIV NotI NruI
PacI PaeR7I PflFI PmaCI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 4291 GACGTC GACGT^C
AccI 1508 GTAGAC GT^[AC][GT]AC
AccII 91 CGCG CG^CG
AflIII 2120 ACATGT A^C[GA][CT]GT
AgeI 829 ACCGGT A^CCGGT
Alw44I 3830 GTGCAC G^TGCAC
ApaLI 3830 GTGCAC G^TGCAC
AseI 446 ATTAAT AT^TAAT
AsnI 446 ATTAAT AT^TAAT
AspHI 3830 GTGCAC G[AT]GC[AT]^C
AvaI 3600 CTCGGG C^[CT]CG[GA]G
BanII 4027 GGGCTC G[GA]GC[CT]^C
BcgI 1172 GCACAGGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1412 GTATCC GTATCC(6/5)
BclI 3148 TGATCA T^GATCA
BglII 949 AGATCT A^GATCT
BsaI 1177 GGTCTC GGTCTC(1/5)
BsaWI 829 ACCGGT [AT]^CCGG[AT]
BseRI 3233 CTCCTC GAGGAG(10/8)
BsiHKAI 3830 GTGCAC G[AT]GC[AT]^C
BsoBI 3600 CTCGGG C^[CT]CG[GA]G
BspLU11I 2120 ACATGT A^CATGT
BstBI 2056 TTCGAA TT^CGAA
BstUI 91 CGCG CG^CG
DrdI 189 GACAAGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3570 CCATGG C^C[GA][CT]GG
EcoRI 544 GAATTC G^AATTC
FbaI 3148 TGATCA T^GATCA
HpaI 1493 GTTAAC GTT^AAC
MaeII 4292 ACGT A^CGT
MvnI 91 CGCG CG^CG
NcoI 3570 CCATGG C^CATGG
NdeI 2271 CATATG CA^TATG
NspV 2056 TTCGAA TT^CGAA
PinAI 829 ACCGGT A^CCGGT
PmeI 3961 GTTTAAAC GTTT^AAAC
PshBI 446 ATTAAT AT^TAAT
PvuII 2617 CAGCTG CAG^CTG
SapI 2674 GCTCTTC GCTCTTC(1/4)
SfiI 133 GGCCGTCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2056 TTCGAA TT^CGAA
SphI 3043 GCATGC GCATG^C
StuI 3683 AGGCCT AGG^CCT
XbaI 3781 TCTAGA T^CTAGA
XcmI 1228 CCATAGACCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Asp700I 615 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2302 GAAAGGCTTC
BalI 169 TGGCCA TGG^CCA
4090 TGGCCA
BbsI 653 GAAGAC GAAGAC(2/6)
2059 GAAGAC
BglI 134 GCCGTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
664 GCCTACTCGGC
BpuAI 653 GAAGAC GAAGAC(2/6)
2059 GAAGAC
BsiEI 115 CGGCCG CG[GA][CT]^CG
127 CGGCCG
BsmBI 102 GAGACG CGTCTC(1/5)
3492 GAGACG
BsmI 343 GAATGC GAATGC(1/-1)
2755 GAATGC
BspDI 3943 ATCGAT AT^CGAT
4314 ATCGAT
BspHI 2480 TCATGA T^CATGA
3484 TCATGA
BsrBI 45 CCGCTC CCGCTC(-3/-3)
61 CCGCTC
BsrFI 829 ACCGGT [GA]^CCGG[CT]
2009 GCCGGT
BstAPI 1855 GCACATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3034 GCAGGGGCTGC
BstEII 261 GGTTACC G^GT[ACGT]ACC
3332 GGTCACC
BstPI 261 GGTTACC G^GT[ACGT]ACC
3332 GGTCACC
Cfr10I 829 ACCGGT [GA]^CCGG[CT]
2009 GCCGGT
ClaI 3943 ATCGAT AT^CGAT
4314 ATCGAT
DraI 3836 TTTAAA TTT^AAA
3962 TTTAAA
DraII 82 GGGACCT [GA]G^G[ACGT]CC[CT]
3749 AGGTCCT
EagI 115 CGGCCG C^GGCCG
127 CGGCCG
EclXI 115 CGGCCG C^GGCCG
127 CGGCCG
Eco52I 115 CGGCCG C^GGCCG
127 CGGCCG
EcoO109I 82 GGGACCT [GA]G^G[ACGT]CC[CT]
3749 AGGTCCT
EcoO65I 261 GGTTACC G^GT[ACGT]ACC
3332 GGTCACC
EcoT22I 4409 ATGCAT ATGCA^T
4604 ATGCAT
MluNI 169 TGGCCA TGG^CCA
4090 TGGCCA
MscI 169 TGGCCA TGG^CCA
4090 TGGCCA
NheI 3030 GCTAGC G^CTAGC
3180 GCTAGC
NsiI 4409 ATGCAT ATGCA^T
4604 ATGCAT
PflMI 775 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1769 CCACCCCCTGG
PpuMI 82 GGGACCT [GA]G^G[AT]CC[CT]
3749 AGGTCCT
PstI 2387 CTGCAG CTGCA^G
3723 CTGCAG
RcaI 2480 TCATGA T^CATGA
3484 TCATGA
SexAI 2880 ACCAGGT A^CC[AT]GGT
4064 ACCTGGT
Van91I 775 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1769 CCACCCCCTGG
XmnI 615 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2302 GAAAGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AvaII BanI
BbeI BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII EaeI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII