##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AgeI AscI AseI AsnI
AvrII BbeI BbrPI BbvCI BglII
BlnI BsaAI BsaBI BsiEI BsiWI
BspDI BspHI BsrDI BssHII Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DraIII EagI EclXI Eco52I EcoRV
EcoT22I FseI HindIII HpaI KasI
MamI MfeI MluI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI PvuI RcaI RsrII
SalI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 3612 GGTACC G^GTACC
AccI 1316 GTCTAC GT^[AC][GT]AC
AccIII 2401 TCCGGA T^CCGGA
AclI 1821 AACGTT AA^CGTT
AflIII 2970 ACATGT A^C[GA][CT]GT
Alw44I 1148 GTGCAC G^TGCAC
Aor51HI 435 AGCGCT AGC^GCT
ApaI 3550 GGGCCC GGGCC^C
ApaLI 1148 GTGCAC G^TGCAC
Asp718I 3612 GGTACC G^GTACC
AspI 752 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 446 GCACGGCGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2772 TGATCA T^GATCA
BlpI 2571 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2571 GCTTAGC GC^T[ACGT]AGC
BsaWI 2401 TCCGGA [AT]^CCGG[AT]
BseAI 2401 TCCGGA T^CCGGA
BsmBI 3094 CGTCTC CGTCTC(1/5)
BsmI 2388 GCATTC GAATGC(1/-1)
Bsp1407I 2441 TGTACA T^GTACA
BspEI 2401 TCCGGA T^CCGGA
BspLU11I 2970 ACATGT A^CATGT
BspMI 1338 ACCTGC ACCTGC(4/8)
BsrGI 2441 TGTACA T^GTACA
BstEII 1864 GGTGACC G^GT[ACGT]ACC
BstPI 1864 GGTGACC G^GT[ACGT]ACC
CelII 2571 GCTTAGC GC^T[ACGT]AGC
DrdI 1307 GACTTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 435 AGCGCT AGC^GCT
EcoNI 2586 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1864 GGTGACC G^GT[ACGT]ACC
EcoRI 3067 GAATTC G^AATTC
FbaI 2772 TGATCA T^GATCA
KpnI 3612 GGTACC GGTAC^C
MroI 2401 TCCGGA T^CCGGA
NaeI 969 GCCGGC GCC^GGC
NgoMIV 969 GCCGGC G^CCGGC
PflFI 752 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2896 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1821 AACGTT AA^CGTT
SapI 1054 GCTCTTC GCTCTTC(1/4)
SbfI 2313 CCTGCAGG CCTGCA^GG
Sse8387I 2313 CCTGCAGG CCTGCA^GG
SspBI 2441 TGTACA T^GTACA
Tth111I 752 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 458 GACGCC G[GA]^CG[CT]C
1079 GACGCC
AflII 1877 CTTAAG C^TTAAG
2982 CTTAAG
AhdI 467 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
626 GACAATGAGTC
Asp700I 3117 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3778 GAATAAATTC
AspEI 467 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
626 GACAATGAGTC
AviII 1068 TGCGCA TGC^GCA
1518 TGCGCA
BalI 1356 TGGCCA TGG^CCA
1830 TGGCCA
BciVI 208 GGATAC GTATCC(6/5)
2966 GGATAC
BfrI 1877 CTTAAG C^TTAAG
2982 CTTAAG
BsaHI 458 GACGCC G[GA]^CG[CT]C
1079 GACGCC
BsgI 3544 CTGCAC GTGCAG(16/14)
2718 GTGCAG
Eam1105I 467 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
626 GACAATGAGTC
FspI 1068 TGCGCA TGC^GCA
1518 TGCGCA
HgaI 458 GACGC GACGC(5/10)
1079 GACGC
Hin1I 458 GACGCC G[GA]^CG[CT]C
1079 GACGCC
KspI 423 CCGCGG CCGC^GG
2042 CCGCGG
MaeII 1822 ACGT A^CGT
3559 ACGT
MluNI 1356 TGGCCA TGG^CCA
1830 TGGCCA
MscI 1356 TGGCCA TGG^CCA
1830 TGGCCA
NspI 2970 ACATGT [GA]CATG^[CT]
2997 ACATGC
PaeR7I 77 CTCGAG C^TCGAG
3743 CTCGAG
PvuII 949 CAGCTG CAG^CTG
2658 CAGCTG
SacII 423 CCGCGG CCGC^GG
2042 CCGCGG
ScaI 480 AGTACT AGT^ACT
1482 AGTACT
XhoI 77 CTCGAG C^TCGAG
3743 CTCGAG
XmnI 3117 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3778 GAATAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BamHI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI