################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII AgeI AscI AseI AsnI AvrII BbeI BbrPI BbvCI BglII BlnI BsaAI BsaBI BsiEI BsiWI BspDI BspHI BsrDI BssHII Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraIII EagI EclXI Eco52I EcoRV EcoT22I FseI HindIII HpaI KasI MamI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshBI PvuI RcaI RsrII SalI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 3612 GGTACC G^GTACC AccI 1316 GTCTAC GT^[AC][GT]AC AccIII 2401 TCCGGA T^CCGGA AclI 1821 AACGTT AA^CGTT AflIII 2970 ACATGT A^C[GA][CT]GT Alw44I 1148 GTGCAC G^TGCAC Aor51HI 435 AGCGCT AGC^GCT ApaI 3550 GGGCCC GGGCC^C ApaLI 1148 GTGCAC G^TGCAC Asp718I 3612 GGTACC G^GTACC AspI 752 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 446 GCACGGCGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2772 TGATCA T^GATCA BlpI 2571 GCTTAGC GC^T[ACGT]AGC Bpu1102I 2571 GCTTAGC GC^T[ACGT]AGC BsaWI 2401 TCCGGA [AT]^CCGG[AT] BseAI 2401 TCCGGA T^CCGGA BsmBI 3094 CGTCTC CGTCTC(1/5) BsmI 2388 GCATTC GAATGC(1/-1) Bsp1407I 2441 TGTACA T^GTACA BspEI 2401 TCCGGA T^CCGGA BspLU11I 2970 ACATGT A^CATGT BspMI 1338 ACCTGC ACCTGC(4/8) BsrGI 2441 TGTACA T^GTACA BstEII 1864 GGTGACC G^GT[ACGT]ACC BstPI 1864 GGTGACC G^GT[ACGT]ACC CelII 2571 GCTTAGC GC^T[ACGT]AGC DrdI 1307 GACTTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 435 AGCGCT AGC^GCT EcoNI 2586 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1864 GGTGACC G^GT[ACGT]ACC EcoRI 3067 GAATTC G^AATTC FbaI 2772 TGATCA T^GATCA KpnI 3612 GGTACC GGTAC^C MroI 2401 TCCGGA T^CCGGA NaeI 969 GCCGGC GCC^GGC NgoMIV 969 GCCGGC G^CCGGC PflFI 752 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2896 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1821 AACGTT AA^CGTT SapI 1054 GCTCTTC GCTCTTC(1/4) SbfI 2313 CCTGCAGG CCTGCA^GG Sse8387I 2313 CCTGCAGG CCTGCA^GG SspBI 2441 TGTACA T^GTACA Tth111I 752 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 458 GACGCC G[GA]^CG[CT]C 1079 GACGCC AflII 1877 CTTAAG C^TTAAG 2982 CTTAAG AhdI 467 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 626 GACAATGAGTC Asp700I 3117 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3778 GAATAAATTC AspEI 467 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 626 GACAATGAGTC AviII 1068 TGCGCA TGC^GCA 1518 TGCGCA BalI 1356 TGGCCA TGG^CCA 1830 TGGCCA BciVI 208 GGATAC GTATCC(6/5) 2966 GGATAC BfrI 1877 CTTAAG C^TTAAG 2982 CTTAAG BsaHI 458 GACGCC G[GA]^CG[CT]C 1079 GACGCC BsgI 3544 CTGCAC GTGCAG(16/14) 2718 GTGCAG Eam1105I 467 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 626 GACAATGAGTC FspI 1068 TGCGCA TGC^GCA 1518 TGCGCA HgaI 458 GACGC GACGC(5/10) 1079 GACGC Hin1I 458 GACGCC G[GA]^CG[CT]C 1079 GACGCC KspI 423 CCGCGG CCGC^GG 2042 CCGCGG MaeII 1822 ACGT A^CGT 3559 ACGT MluNI 1356 TGGCCA TGG^CCA 1830 TGGCCA MscI 1356 TGGCCA TGG^CCA 1830 TGGCCA NspI 2970 ACATGT [GA]CATG^[CT] 2997 ACATGC PaeR7I 77 CTCGAG C^TCGAG 3743 CTCGAG PvuII 949 CAGCTG CAG^CTG 2658 CAGCTG SacII 423 CCGCGG CCGC^GG 2042 CCGCGG ScaI 480 AGTACT AGT^ACT 1482 AGTACT XhoI 77 CTCGAG C^TCGAG 3743 CTCGAG XmnI 3117 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3778 GAATAAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BamHI BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI