##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AccIII AclI AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AviII BamHI BbvCI BcgI
BciVI BclI BsaWI BseAI BsiWI
BspEI BssHII Bst1107I BstAPI BstBI
BstZ17I CpoI Eco47III EcoRV FbaI
FspI HpaI KpnI MfeI MluI
MroI MunI NdeI NruI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 2495 GACGTC GACGT^C
AcsI 3891 GAATTC [GA]^AATT[CT]
AflII 3739 CTTAAG C^TTAAG
AhdI 1705 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 3891 GAATTC [GA]^AATT[CT]
Asp700I 4000 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1705 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2702 CACGTG CAC^GTG
BbsI 3295 GTCTTC GAAGAC(2/6)
BfrI 3739 CTTAAG C^TTAAG
BpuAI 3295 GTCTTC GAAGAC(2/6)
BsaBI 2479 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 408 GAGACG CGTCTC(1/5)
BspDI 1227 ATCGAT AT^CGAT
BspHI 1962 TCATGA T^CATGA
BspLU11I 3110 ACATGT A^CATGT
BsrBI 1275 GAGCGG CCGCTC(-3/-3)
BssSI 904 CACGAG CACGAG(-5/-1)
ClaI 1227 ATCGAT AT^CGAT
DraIII 2232 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2921 GACAAGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1705 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3435 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3891 GAATTC G^AATTC
FseI 248 GGCCGGCC GGCCGG^CC
HindIII 4134 AAGCTT A^AGCTT
MamI 2479 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI 21 GCGGCCGC GC^GGCCGC
PmaCI 2702 CACGTG CAC^GTG
PmlI 2702 CACGTG CAC^GTG
RcaI 1962 TCATGA T^CATGA
XbaI 1836 TCTAGA T^CTAGA
XmnI 4000 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 2944 GTCTAC GT^[AC][GT]AC
4572 GTAGAC
AocI 1617 CCTAAGG CC^T[ACGT]AGG
2785 CCTCAGG
AspI 1649 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4603 GACAGTGTC
AvrII 873 CCTAGG C^CTAGG
3772 CCTAGG
BbeI 713 GGCGCC GGCGC^C
1320 GGCGCC
BglI 258 GCCGCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2872 GCCCACCAGGC
BlnI 873 CCTAGG C^CTAGG
3772 CCTAGG
BsaAI 1421 CACGTA [CT]AC^GT[GA]
2702 CACGTG
BsaI 1101 GAGACC GGTCTC(1/5)
2646 GGTCTC
BsgI 454 CTGCAC GTGCAG(16/14)
2013 CTGCAC
Bsu36I 1617 CCTAAGG CC^T[ACGT]AGG
2785 CCTCAGG
DraI 2995 TTTAAA TTT^AAA
3159 TTTAAA
Eco81I 1617 CCTAAGG CC^T[ACGT]AGG
2785 CCTCAGG
HincII 882 GTCAAC GT[CT]^[GA]AC
2130 GTCAAC
HindII 882 GTCAAC GT[CT]^[GA]AC
2130 GTCAAC
KasI 713 GGCGCC G^GCGCC
1320 GGCGCC
NarI 713 GGCGCC GG^CGCC
1320 GGCGCC
PflFI 1649 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4603 GACAGTGTC
SacI 1958 GAGCTC GAGCT^C
3045 GAGCTC
SapI 1262 GAAGAGC GCTCTTC(1/4)
4126 GAAGAGC
SfoI 713 GGCGCC GGC^GCC
1320 GGCGCC
Tth111I 1649 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4603 GACAGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglII BlpI
BmyI BpmI Bpu1102I BsaHI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrDI BsrFI BsrGI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoO65I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NheI NlaIII NlaIV NsiI NspI
PaeR7I PflMI PleI PpuMI PshAI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoI
XhoII XmaCI XmaI