################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AclI AflII AscI AspI AviII AvrII BamHI BbeI BcgI BciVI BfrI BlnI BsaBI BsiWI BsmBI BspDI BspLU11I BspMI BssHII ClaI CpoI DraI DraIII EcoRV FseI FspI HindIII HpaI KasI KspI MamI MfeI MluI MunI NarI NotI NruI PacI PaeR7I PflFI PmeI PpuMI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SfoI SgrAI SpeI Sse8387I SspI StuI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 596 GACGTC GACGT^C Acc65I 3193 GGTACC G^GTACC AccIII 121 TCCGGA T^CCGGA AgeI 4473 ACCGGT A^CCGGT AocI 2877 CCTGAGG CC^T[ACGT]AGG Aor51HI 3218 AGCGCT AGC^GCT AseI 791 ATTAAT AT^TAAT AsnI 791 ATTAAT AT^TAAT Asp718I 3193 GGTACC G^GTACC BclI 853 TGATCA T^GATCA BglII 991 AGATCT A^GATCT BlpI 2738 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2738 GCTGAGC GC^T[ACGT]AGC BsaI 2823 GAGACC GGTCTC(1/5) BseAI 121 TCCGGA T^CCGGA BsiEI 500 CGGCCG CG[GA][CT]^CG BspEI 121 TCCGGA T^CCGGA BspHI 91 TCATGA T^CATGA BsrBI 539 GAGCGG CCGCTC(-3/-3) BstBI 3344 TTCGAA TT^CGAA Bsu36I 2877 CCTGAGG CC^T[ACGT]AGG CelII 2738 GCTGAGC GC^T[ACGT]AGC DrdI 275 GACTGGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 500 CGGCCG C^GGCCG EclXI 500 CGGCCG C^GGCCG Eco47III 3218 AGCGCT AGC^GCT Eco52I 500 CGGCCG C^GGCCG Eco81I 2877 CCTGAGG CC^T[ACGT]AGG EcoRI 2016 GAATTC G^AATTC EcoT22I 3413 ATGCAT ATGCA^T FbaI 853 TGATCA T^GATCA KpnI 3193 GGTACC GGTAC^C MroI 121 TCCGGA T^CCGGA NdeI 865 CATATG CA^TATG NheI 179 GCTAGC G^CTAGC NsiI 3413 ATGCAT ATGCA^T NspV 3344 TTCGAA TT^CGAA PinAI 4473 ACCGGT A^CCGGT PshBI 791 ATTAAT AT^TAAT RcaI 91 TCATGA T^CATGA ScaI 364 AGTACT AGT^ACT SfiI 2033 GGCCCAACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 3344 TTCGAA TT^CGAA SmaI 3916 CCCGGG CCC^GGG SphI 2248 GCATGC GCATG^C XmaCI 3916 CCCGGG C^CCGGG XmaI 3916 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 3057 GTATAC GT^[AC][GT]AC 4398 GTATAC AccII 376 CGCG CG^CG 3190 CGCG Alw44I 1680 GTGCAC G^TGCAC 3661 GTGCAC ApaLI 1680 GTGCAC G^TGCAC 3661 GTGCAC Asp700I 2931 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3072 GAAGAGCTTC BalI 1216 TGGCCA TGG^CCA 2957 TGGCCA BbrPI 2687 CACGTG CAC^GTG 3666 CACGTG BglI 2034 GCCCAACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3273 GCCATCAAGGC Bsp1407I 3358 TGTACA T^GTACA 3710 TGTACA BsrDI 140 CATTGC GCAATG(2/0) 2477 CATTGC BsrGI 3358 TGTACA T^GTACA 3710 TGTACA BssSI 1875 CTCGTG CACGAG(-5/-1) 3784 CTCGTG Bst1107I 3057 GTATAC GTA^TAC 4398 GTATAC BstEII 827 GGTGACC G^GT[ACGT]ACC 870 GGTTACC BstPI 827 GGTGACC G^GT[ACGT]ACC 870 GGTTACC BstUI 376 CGCG CG^CG 3190 CGCG BstZ17I 3057 GTATAC GTA^TAC 4398 GTATAC EcoO65I 827 GGTGACC G^GT[ACGT]ACC 870 GGTTACC MluNI 1216 TGGCCA TGG^CCA 2957 TGGCCA MscI 1216 TGGCCA TGG^CCA 2957 TGGCCA MvnI 376 CGCG CG^CG 3190 CGCG PmaCI 2687 CACGTG CAC^GTG 3666 CACGTG PmlI 2687 CACGTG CAC^GTG 3666 CACGTG SacI 163 GAGCTC GAGCT^C 1287 GAGCTC SexAI 523 ACCTGGT A^CC[AT]GGT 3007 ACCAGGT SnaBI 3768 TACGTA TAC^GTA 4438 TACGTA SspBI 3358 TGTACA T^GTACA 3710 TGTACA XbaI 2456 TCTAGA T^CTAGA 3096 TCTAGA XcmI 259 CCATCCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 514 CCATCCTGGACCTGG XmnI 2931 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3072 GAAGAGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AhdI AluI AlwI AlwNI ApaI ApoI AspEI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII