##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AclI AflII AscI AspI AviII
AvrII BamHI BbeI BcgI BciVI
BfrI BlnI BsaBI BsiWI BsmBI
BspDI BspLU11I BspMI BssHII ClaI
CpoI DraI DraIII EcoRV FseI
FspI HindIII HpaI KasI KspI
MamI MfeI MluI MunI NarI
NotI NruI PacI PaeR7I PflFI
PmeI PpuMI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI SfoI
SgrAI SpeI Sse8387I SspI StuI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 596 GACGTC GACGT^C
Acc65I 3193 GGTACC G^GTACC
AccIII 121 TCCGGA T^CCGGA
AgeI 4473 ACCGGT A^CCGGT
AocI 2877 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3218 AGCGCT AGC^GCT
AseI 791 ATTAAT AT^TAAT
AsnI 791 ATTAAT AT^TAAT
Asp718I 3193 GGTACC G^GTACC
BclI 853 TGATCA T^GATCA
BglII 991 AGATCT A^GATCT
BlpI 2738 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2738 GCTGAGC GC^T[ACGT]AGC
BsaI 2823 GAGACC GGTCTC(1/5)
BseAI 121 TCCGGA T^CCGGA
BsiEI 500 CGGCCG CG[GA][CT]^CG
BspEI 121 TCCGGA T^CCGGA
BspHI 91 TCATGA T^CATGA
BsrBI 539 GAGCGG CCGCTC(-3/-3)
BstBI 3344 TTCGAA TT^CGAA
Bsu36I 2877 CCTGAGG CC^T[ACGT]AGG
CelII 2738 GCTGAGC GC^T[ACGT]AGC
DrdI 275 GACTGGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 500 CGGCCG C^GGCCG
EclXI 500 CGGCCG C^GGCCG
Eco47III 3218 AGCGCT AGC^GCT
Eco52I 500 CGGCCG C^GGCCG
Eco81I 2877 CCTGAGG CC^T[ACGT]AGG
EcoRI 2016 GAATTC G^AATTC
EcoT22I 3413 ATGCAT ATGCA^T
FbaI 853 TGATCA T^GATCA
KpnI 3193 GGTACC GGTAC^C
MroI 121 TCCGGA T^CCGGA
NdeI 865 CATATG CA^TATG
NheI 179 GCTAGC G^CTAGC
NsiI 3413 ATGCAT ATGCA^T
NspV 3344 TTCGAA TT^CGAA
PinAI 4473 ACCGGT A^CCGGT
PshBI 791 ATTAAT AT^TAAT
RcaI 91 TCATGA T^CATGA
ScaI 364 AGTACT AGT^ACT
SfiI 2033 GGCCCAACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3344 TTCGAA TT^CGAA
SmaI 3916 CCCGGG CCC^GGG
SphI 2248 GCATGC GCATG^C
XmaCI 3916 CCCGGG C^CCGGG
XmaI 3916 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 3057 GTATAC GT^[AC][GT]AC
4398 GTATAC
AccII 376 CGCG CG^CG
3190 CGCG
Alw44I 1680 GTGCAC G^TGCAC
3661 GTGCAC
ApaLI 1680 GTGCAC G^TGCAC
3661 GTGCAC
Asp700I 2931 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3072 GAAGAGCTTC
BalI 1216 TGGCCA TGG^CCA
2957 TGGCCA
BbrPI 2687 CACGTG CAC^GTG
3666 CACGTG
BglI 2034 GCCCAACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3273 GCCATCAAGGC
Bsp1407I 3358 TGTACA T^GTACA
3710 TGTACA
BsrDI 140 CATTGC GCAATG(2/0)
2477 CATTGC
BsrGI 3358 TGTACA T^GTACA
3710 TGTACA
BssSI 1875 CTCGTG CACGAG(-5/-1)
3784 CTCGTG
Bst1107I 3057 GTATAC GTA^TAC
4398 GTATAC
BstEII 827 GGTGACC G^GT[ACGT]ACC
870 GGTTACC
BstPI 827 GGTGACC G^GT[ACGT]ACC
870 GGTTACC
BstUI 376 CGCG CG^CG
3190 CGCG
BstZ17I 3057 GTATAC GTA^TAC
4398 GTATAC
EcoO65I 827 GGTGACC G^GT[ACGT]ACC
870 GGTTACC
MluNI 1216 TGGCCA TGG^CCA
2957 TGGCCA
MscI 1216 TGGCCA TGG^CCA
2957 TGGCCA
MvnI 376 CGCG CG^CG
3190 CGCG
PmaCI 2687 CACGTG CAC^GTG
3666 CACGTG
PmlI 2687 CACGTG CAC^GTG
3666 CACGTG
SacI 163 GAGCTC GAGCT^C
1287 GAGCTC
SexAI 523 ACCTGGT A^CC[AT]GGT
3007 ACCAGGT
SnaBI 3768 TACGTA TAC^GTA
4438 TACGTA
SspBI 3358 TGTACA T^GTACA
3710 TGTACA
XbaI 2456 TCTAGA T^CTAGA
3096 TCTAGA
XcmI 259 CCATCCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
514 CCATCCTGGACCTGG
XmnI 2931 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3072 GAAGAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AhdI AluI AlwI AlwNI ApaI
ApoI AspEI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII