################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AgeI AocI ApaI AscI Asp718I AviII BalI BamHI BcgI BfrI BglII BlpI Bpu1102I BsaWI BseAI BsgI BsiWI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI EagI EclXI Eco52I Eco81I EcoNI EcoRI FseI FspI HindIII KpnI KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NgoMIV NheI NotI NruI NspV PacI PflMI PinAI PmeI PshAI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SnaBI Sse8387I SwaI Van91I XbaI XcmI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 2540 GACGTC GACGT^C AccI 185 GTCTAC GT^[AC][GT]AC AhdI 217 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1852 AGCGCT AGC^GCT AspEI 217 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2286 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 1700 GGCGCC GGCGC^C BbrPI 2301 CACGTG CAC^GTG BclI 3332 TGATCA T^GATCA BglI 3555 GCCTTCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 2301 CACGTG [CT]AC^GT[GA] BsaBI 951 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 2936 CGGTCG CG[GA][CT]^CG BsmI 398 GAATGC GAATGC(1/-1) BsrDI 2776 GCAATG GCAATG(2/0) BstEII 2972 GGTGACC G^GT[ACGT]ACC BstPI 2972 GGTGACC G^GT[ACGT]ACC BstYI 1552 GGATCT [GA]^GATC[CT] DraIII 2468 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 537 TGGCCG [CT]^GGCC[GA] Eam1105I 217 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1852 AGCGCT AGC^GCT EcoO65I 2972 GGTGACC G^GT[ACGT]ACC EcoRV 3747 GATATC GAT^ATC FbaI 3332 TGATCA T^GATCA HgaI 1951 GACGC GACGC(5/10) HpaI 2709 GTTAAC GTT^AAC KasI 1700 GGCGCC G^GCGCC MamI 951 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1552 GGATCT [GA]^GATC[CT] MspA1I 2022 CCGCTG C[AC]G^C[GT]G NarI 1700 GGCGCC GG^CGCC PflFI 2286 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2301 CACGTG CAC^GTG PmlI 2301 CACGTG CAC^GTG ScaI 544 AGTACT AGT^ACT SfoI 1700 GGCGCC GGC^GCC SpeI 2713 ACTAGT A^CTAGT SphI 1175 GCATGC GCATG^C Tth111I 2286 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 1552 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AcyI 1700 GGCGCC G[GA]^CG[CT]C 2540 GACGTC Alw44I 1588 GTGCAC G^TGCAC 2815 GTGCAC AlwI 2352 GATCC GGATC(4/5) 1552 GGATC AlwNI 2890 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 3546 CAGAGCCTG ApaLI 1588 GTGCAC G^TGCAC 2815 GTGCAC AseI 2798 ATTAAT AT^TAAT 3728 ATTAAT AsnI 2798 ATTAAT AT^TAAT 3728 ATTAAT AvrII 450 CCTAGG C^CTAGG 2313 CCTAGG BanI 1700 GGCGCC G^G[CT][GA]CC 2865 GGTGCC BanII 882 GAGCTC G[GA]GC[CT]^C 1624 GAGCTC BbvCI 706 CCTCAGC CCTCAGC(-5/-2) 3115 CCTCAGC BciVI 58 GTATCC GTATCC(6/5) 3057 GTATCC BlnI 450 CCTAGG C^CTAGG 2313 CCTAGG BsaHI 1700 GGCGCC G[GA]^CG[CT]C 2540 GACGTC BsmBI 1322 CGTCTC CGTCTC(1/5) 2542 CGTCTC BspMI 20 ACCTGC ACCTGC(4/8) 2889 GCAGGT BstAPI 1170 GCAAGGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2889 GCAGGTGCTGC BstXI 2496 CCACAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3046 CCATCTGTGTGG DsaI 243 CCATGG C^C[GA][CT]GG 3072 CCATGG EcoT22I 3379 ATGCAT ATGCA^T 3641 ATGCAT FauI 2068 CCCGC CCCGC(4/6) 3129 CCCGC HaeII 1700 GGCGCC [GA]GCGC^[CT] 1852 AGCGCT Hin1I 1700 GGCGCC G[GA]^CG[CT]C 2540 GACGTC NcoI 243 CCATGG C^CATGG 3072 CCATGG NdeI 3328 CATATG CA^TATG 3376 CATATG NsiI 3379 ATGCAT ATGCA^T 3641 ATGCAT PaeR7I 1328 CTCGAG C^TCGAG 1627 CTCGAG PpuMI 933 AGGACCT [GA]G^G[AT]CC[CT] 1260 GGGTCCT PshBI 2798 ATTAAT AT^TAAT 3728 ATTAAT SacI 882 GAGCTC GAGCT^C 1624 GAGCTC SmaI 1407 CCCGGG CCC^GGG 3145 CCCGGG XhoI 1328 CTCGAG C^TCGAG 1627 CTCGAG XmaCI 1407 CCCGGG C^CCGGG 3145 CCCGGG XmaI 1407 CCCGGG C^CCGGG 3145 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI ApoI Asp700I AspHI AvaI AvaII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspLU11I BsrGI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI