##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AocI ApaI AscI Asp718I
AviII BalI BamHI BcgI BfrI
BglII BlpI Bpu1102I BsaWI BseAI
BsgI BsiWI BspDI BspEI BspHI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco52I Eco81I EcoNI EcoRI
FseI FspI HindIII KpnI KspI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NgoMIV NheI
NotI NruI NspV PacI PflMI
PinAI PmeI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI Sse8387I SwaI Van91I
XbaI XcmI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 2540 GACGTC GACGT^C
AccI 185 GTCTAC GT^[AC][GT]AC
AhdI 217 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1852 AGCGCT AGC^GCT
AspEI 217 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2286 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1700 GGCGCC GGCGC^C
BbrPI 2301 CACGTG CAC^GTG
BclI 3332 TGATCA T^GATCA
BglI 3555 GCCTTCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2301 CACGTG [CT]AC^GT[GA]
BsaBI 951 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2936 CGGTCG CG[GA][CT]^CG
BsmI 398 GAATGC GAATGC(1/-1)
BsrDI 2776 GCAATG GCAATG(2/0)
BstEII 2972 GGTGACC G^GT[ACGT]ACC
BstPI 2972 GGTGACC G^GT[ACGT]ACC
BstYI 1552 GGATCT [GA]^GATC[CT]
DraIII 2468 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 537 TGGCCG [CT]^GGCC[GA]
Eam1105I 217 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1852 AGCGCT AGC^GCT
EcoO65I 2972 GGTGACC G^GT[ACGT]ACC
EcoRV 3747 GATATC GAT^ATC
FbaI 3332 TGATCA T^GATCA
HgaI 1951 GACGC GACGC(5/10)
HpaI 2709 GTTAAC GTT^AAC
KasI 1700 GGCGCC G^GCGCC
MamI 951 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1552 GGATCT [GA]^GATC[CT]
MspA1I 2022 CCGCTG C[AC]G^C[GT]G
NarI 1700 GGCGCC GG^CGCC
PflFI 2286 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2301 CACGTG CAC^GTG
PmlI 2301 CACGTG CAC^GTG
ScaI 544 AGTACT AGT^ACT
SfoI 1700 GGCGCC GGC^GCC
SpeI 2713 ACTAGT A^CTAGT
SphI 1175 GCATGC GCATG^C
Tth111I 2286 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1552 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 1700 GGCGCC G[GA]^CG[CT]C
2540 GACGTC
Alw44I 1588 GTGCAC G^TGCAC
2815 GTGCAC
AlwI 2352 GATCC GGATC(4/5)
1552 GGATC
AlwNI 2890 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
3546 CAGAGCCTG
ApaLI 1588 GTGCAC G^TGCAC
2815 GTGCAC
AseI 2798 ATTAAT AT^TAAT
3728 ATTAAT
AsnI 2798 ATTAAT AT^TAAT
3728 ATTAAT
AvrII 450 CCTAGG C^CTAGG
2313 CCTAGG
BanI 1700 GGCGCC G^G[CT][GA]CC
2865 GGTGCC
BanII 882 GAGCTC G[GA]GC[CT]^C
1624 GAGCTC
BbvCI 706 CCTCAGC CCTCAGC(-5/-2)
3115 CCTCAGC
BciVI 58 GTATCC GTATCC(6/5)
3057 GTATCC
BlnI 450 CCTAGG C^CTAGG
2313 CCTAGG
BsaHI 1700 GGCGCC G[GA]^CG[CT]C
2540 GACGTC
BsmBI 1322 CGTCTC CGTCTC(1/5)
2542 CGTCTC
BspMI 20 ACCTGC ACCTGC(4/8)
2889 GCAGGT
BstAPI 1170 GCAAGGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2889 GCAGGTGCTGC
BstXI 2496 CCACAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3046 CCATCTGTGTGG
DsaI 243 CCATGG C^C[GA][CT]GG
3072 CCATGG
EcoT22I 3379 ATGCAT ATGCA^T
3641 ATGCAT
FauI 2068 CCCGC CCCGC(4/6)
3129 CCCGC
HaeII 1700 GGCGCC [GA]GCGC^[CT]
1852 AGCGCT
Hin1I 1700 GGCGCC G[GA]^CG[CT]C
2540 GACGTC
NcoI 243 CCATGG C^CATGG
3072 CCATGG
NdeI 3328 CATATG CA^TATG
3376 CATATG
NsiI 3379 ATGCAT ATGCA^T
3641 ATGCAT
PaeR7I 1328 CTCGAG C^TCGAG
1627 CTCGAG
PpuMI 933 AGGACCT [GA]G^G[AT]CC[CT]
1260 GGGTCCT
PshBI 2798 ATTAAT AT^TAAT
3728 ATTAAT
SacI 882 GAGCTC GAGCT^C
1624 GAGCTC
SmaI 1407 CCCGGG CCC^GGG
3145 CCCGGG
XhoI 1328 CTCGAG C^TCGAG
1627 CTCGAG
XmaCI 1407 CCCGGG C^CCGGG
3145 CCCGGG
XmaI 1407 CCCGGG C^CCGGG
3145 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
ApoI Asp700I AspHI AvaI AvaII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspLU11I BsrGI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmnI