##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BfrI BlnI BsaBI BsaWI BseAI
BsiWI BsmBI BspDI BspEI BspLU11I
BssSI BstEII BstPI ClaI CpoI
DrdI Eam1105I EcoO65I EcoRV FseI
FspI HpaI MamI MfeI MluI
MroI MunI NaeI NgoMIV NheI
NruI PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SbfI SexAI
SfiI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 1110 GGCGCC G[GA]^CG[CT]C
AflIII 4391 ACGTGT A^C[GA][CT]GT
Aor51HI 4814 AGCGCT AGC^GCT
AvaI 81 CCCGAG C^[CT]CG[GA]G
BamHI 3791 GGATCC G^GATCC
BbeI 1110 GGCGCC GGCGC^C
BbrPI 4543 CACGTG CAC^GTG
BglI 3843 GCCCAGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 154 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 154 GCTAAGC GC^T[ACGT]AGC
BsaHI 1110 GGCGCC G[GA]^CG[CT]C
BsiEI 280 CGGCCG CG[GA][CT]^CG
BsoBI 81 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2046 TGTACA T^GTACA
BspHI 852 TCATGA T^CATGA
BsrGI 2046 TGTACA T^GTACA
BssHII 159 GCGCGC G^CGCGC
CelII 154 GCTAAGC GC^T[ACGT]AGC
DraIII 1910 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 280 CGGCCG C^GGCCG
EclXI 280 CGGCCG C^GGCCG
Eco47III 4814 AGCGCT AGC^GCT
Eco52I 280 CGGCCG C^GGCCG
EcoNI 1365 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2727 ATGCAT ATGCA^T
Hin1I 1110 GGCGCC G[GA]^CG[CT]C
KasI 1110 GGCGCC G^GCGCC
NarI 1110 GGCGCC GG^CGCC
NcoI 2924 CCATGG C^CATGG
NdeI 1595 CATATG CA^TATG
NotI 279 GCGGCCGC GC^GGCCGC
NsiI 2727 ATGCAT ATGCA^T
PmaCI 4543 CACGTG CAC^GTG
PmlI 4543 CACGTG CAC^GTG
PpuMI 136 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 852 TCATGA T^CATGA
ScaI 3097 AGTACT AGT^ACT
SfoI 1110 GGCGCC GGC^GCC
SgrAI 240 CGCCGGTG C[GA]^CCGG[CT]G
SspBI 2046 TGTACA T^GTACA
XbaI 3555 TCTAGA T^CTAGA
XcmI 5194 CCAGTTTCTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 3267 GGTACC G^GTACC
3873 GGTACC
ApaI 57 GGGCCC GGGCC^C
316 GGGCCC
Asp718I 3267 GGTACC G^GTACC
3873 GGTACC
BalI 1176 TGGCCA TGG^CCA
2762 TGGCCA
BciVI 420 GGATAC GTATCC(6/5)
1438 GGATAC
BclI 747 TGATCA T^GATCA
1529 TGATCA
BglII 3459 AGATCT A^GATCT
4011 AGATCT
BsaAI 4390 TACGTG [CT]AC^GT[GA]
4543 CACGTG
BsrFI 241 GCCGGT [GA]^CCGG[CT]
346 ACCGGC
BstAPI 1655 GCAGCATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2123 GCAACTTCTGC
BstBI 1740 TTCGAA TT^CGAA
5181 TTCGAA
Cfr10I 241 GCCGGT [GA]^CCGG[CT]
346 ACCGGC
DraI 763 TTTAAA TTT^AAA
1512 TTTAAA
FbaI 747 TGATCA T^GATCA
1529 TGATCA
HgaI 4890 GACGC GACGC(5/10)
3044 GCGTC
HindIII 2374 AAGCTT A^AGCTT
3158 AAGCTT
KpnI 3267 GGTACC GGTAC^C
3873 GGTACC
KspI 277 CCGCGG CCGC^GG
301 CCGCGG
MluNI 1176 TGGCCA TGG^CCA
2762 TGGCCA
MscI 1176 TGGCCA TGG^CCA
2762 TGGCCA
NspV 1740 TTCGAA TT^CGAA
5181 TTCGAA
SacII 277 CCGCGG CCGC^GG
301 CCGCGG
SfuI 1740 TTCGAA TT^CGAA
5181 TTCGAA
SspI 1060 AATATT AAT^ATT
5247 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaII BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
Bst1107I BstF5I BstNI BstUI BstXI
BstYI BstZ17I Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI