################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BfrI BlnI BsaBI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspLU11I BssSI BstEII BstPI ClaI CpoI DrdI Eam1105I EcoO65I EcoRV FseI FspI HpaI MamI MfeI MluI MroI MunI NaeI NgoMIV NheI NruI PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SbfI SexAI SfiI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcyI 1110 GGCGCC G[GA]^CG[CT]C AflIII 4391 ACGTGT A^C[GA][CT]GT Aor51HI 4814 AGCGCT AGC^GCT AvaI 81 CCCGAG C^[CT]CG[GA]G BamHI 3791 GGATCC G^GATCC BbeI 1110 GGCGCC GGCGC^C BbrPI 4543 CACGTG CAC^GTG BglI 3843 GCCCAGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 154 GCTAAGC GC^T[ACGT]AGC Bpu1102I 154 GCTAAGC GC^T[ACGT]AGC BsaHI 1110 GGCGCC G[GA]^CG[CT]C BsiEI 280 CGGCCG CG[GA][CT]^CG BsoBI 81 CCCGAG C^[CT]CG[GA]G Bsp1407I 2046 TGTACA T^GTACA BspHI 852 TCATGA T^CATGA BsrGI 2046 TGTACA T^GTACA BssHII 159 GCGCGC G^CGCGC CelII 154 GCTAAGC GC^T[ACGT]AGC DraIII 1910 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 280 CGGCCG C^GGCCG EclXI 280 CGGCCG C^GGCCG Eco47III 4814 AGCGCT AGC^GCT Eco52I 280 CGGCCG C^GGCCG EcoNI 1365 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 2727 ATGCAT ATGCA^T Hin1I 1110 GGCGCC G[GA]^CG[CT]C KasI 1110 GGCGCC G^GCGCC NarI 1110 GGCGCC GG^CGCC NcoI 2924 CCATGG C^CATGG NdeI 1595 CATATG CA^TATG NotI 279 GCGGCCGC GC^GGCCGC NsiI 2727 ATGCAT ATGCA^T PmaCI 4543 CACGTG CAC^GTG PmlI 4543 CACGTG CAC^GTG PpuMI 136 GGGACCC [GA]G^G[AT]CC[CT] RcaI 852 TCATGA T^CATGA ScaI 3097 AGTACT AGT^ACT SfoI 1110 GGCGCC GGC^GCC SgrAI 240 CGCCGGTG C[GA]^CCGG[CT]G SspBI 2046 TGTACA T^GTACA XbaI 3555 TCTAGA T^CTAGA XcmI 5194 CCAGTTTCTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Acc65I 3267 GGTACC G^GTACC 3873 GGTACC ApaI 57 GGGCCC GGGCC^C 316 GGGCCC Asp718I 3267 GGTACC G^GTACC 3873 GGTACC BalI 1176 TGGCCA TGG^CCA 2762 TGGCCA BciVI 420 GGATAC GTATCC(6/5) 1438 GGATAC BclI 747 TGATCA T^GATCA 1529 TGATCA BglII 3459 AGATCT A^GATCT 4011 AGATCT BsaAI 4390 TACGTG [CT]AC^GT[GA] 4543 CACGTG BsrFI 241 GCCGGT [GA]^CCGG[CT] 346 ACCGGC BstAPI 1655 GCAGCATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2123 GCAACTTCTGC BstBI 1740 TTCGAA TT^CGAA 5181 TTCGAA Cfr10I 241 GCCGGT [GA]^CCGG[CT] 346 ACCGGC DraI 763 TTTAAA TTT^AAA 1512 TTTAAA FbaI 747 TGATCA T^GATCA 1529 TGATCA HgaI 4890 GACGC GACGC(5/10) 3044 GCGTC HindIII 2374 AAGCTT A^AGCTT 3158 AAGCTT KpnI 3267 GGTACC GGTAC^C 3873 GGTACC KspI 277 CCGCGG CCGC^GG 301 CCGCGG MluNI 1176 TGGCCA TGG^CCA 2762 TGGCCA MscI 1176 TGGCCA TGG^CCA 2762 TGGCCA NspV 1740 TTCGAA TT^CGAA 5181 TTCGAA SacII 277 CCGCGG CCGC^GG 301 CCGCGG SfuI 1740 TTCGAA TT^CGAA 5181 TTCGAA SspI 1060 AATATT AAT^ATT 5247 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaII BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI Bst1107I BstF5I BstNI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI